CF834419

Overview
NameCF834419
Unique NameCF834419
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length803
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF834419 vs. ExPASy Swiss-Prot
Match: ALA2_PANMI (Alanine aminotransferase 2 OS=Panicum miliaceum PE=1 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 2.849e-56
Identity = 111/221 (50.23%), Postives = 155/221 (70.14%), Query Frame = 1
Query:  169 ESMNENVKKVQYAVRGELYLRASELQKEGK---------KIIFTNVGNPHALGQRPLTFPRQVVALCQAPFLLDDPNVGIVFPADAIARAKHYLSLTSG-GLGAYSDSRGIPGVRKEVAEFIERRDGYPSDPELIFLTDGASKGVMQTLNCVIRGEGDGVLVPVPQYPLYSATISLLGGSLVPYYLEETANWGLDLNDLQQSVAQARSKGITVRAMVIINP 801
            E++N  V K +YAVRGE+ + A  LQ++ +         +I++ N+GNP +LGQ+P+TF R+V+ALC  P LL+      +F ADAI+RAK  LS   G   GAYS S+GI G+R  +A  I  RDG+P++ + IF+TDGAS GV   +  +IR E DG+L P+PQYPLYSA+I+L GG+LVPYYL+E   WGL+++DL++ +  ARSKGI VRA+V+INP
Sbjct:    8 ENLNPKVLKCEYAVRGEIVIHAQHLQQQLQTQPGSLPFDEILYCNIGNPQSLGQQPVTFFREVLALCDHPCLLEKEETKSLFSADAISRAKQILSTIPGRATGAYSHSQGIKGLRDAIAAGIASRDGFPANADDIFVTDGASPGVHMMMQLLIRNEKDGILCPIPQYPLYSASIALHGGTLVPYYLDEKTGWGLEISDLKKQLEDARSKGIDVRALVVINP 228          
BLAST of CF834419 vs. ExPASy Swiss-Prot
Match: ALA2_HORVU (Alanine aminotransferase 2 OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 6.347e-56
Identity = 109/221 (49.32%), Postives = 156/221 (70.59%), Query Frame = 1
Query:  169 ESMNENVKKVQYAVRGELYLRASELQKEGK---------KIIFTNVGNPHALGQRPLTFPRQVVALCQAPFLLDDPNVGIVFPADAIARAKHYLSLTSG-GLGAYSDSRGIPGVRKEVAEFIERRDGYPSDPELIFLTDGASKGVMQTLNCVIRGEGDGVLVPVPQYPLYSATISLLGGSLVPYYLEETANWGLDLNDLQQSVAQARSKGITVRAMVIINP 801
            +++N  V K +YAVRGE+ + A  LQ++ K         +I++ N+GNP +LGQ+P+TF R+V+ALC  P LL    +  +F AD+I+RAK  L++  G   GAYS S+GI G+R  +A  I  RDG+P++ + IFLTDGAS GV   +  +IR E DG+LVP+PQYPLYSA+I+L GG+LVPYYL E+  WGL+ +D+++ +  ARS+GI VRA+V+INP
Sbjct:    8 DNLNPKVLKCEYAVRGEIVIHAQRLQEQLKTQPGSLPFDEILYCNIGNPQSLGQQPVTFFREVLALCDHPDLLQREEIKTLFSADSISRAKQILAMIPGRATGAYSHSQGIKGLRDAIASGIASRDGFPANADDIFLTDGASPGVHLMMQLLIRNEKDGILVPIPQYPLYSASIALHGGALVPYYLNESTGWGLETSDVKKQLEDARSRGINVRALVVINP 228          
BLAST of CF834419 vs. ExPASy Swiss-Prot
Match: ALAM_DICDI (Probable alanine aminotransferase, mitochondrial OS=Dictyostelium discoideum GN=gpt PE=3 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 4.114e-55
Identity = 116/229 (50.66%), Postives = 154/229 (67.25%), Query Frame = 1
Query:  154 KALDYESMNENVKKVQYAVRGELYLRAS----ELQK---EGKK------IIFTNVGNPHALGQRPLTFPRQVVALCQAPFLLDDPNVGIVFPADAIARAKHYLSLTSGGLGAYSDSRGIPGVRKEVAEFIERRDGYPSDPELIFLTDGASKGVMQTLNCVIRGEGDGVLVPVPQYPLYSATISLLGGSLVPYYLEETANWGLDLNDLQQSVAQARSKGITVRAMVIINP 801
            K++  +++ +NV+  QYAVRGEL +RA     +LQK   EG K      I++ N+GNP  L Q+PLT+ RQVV+L + P LLD+P V  ++PAD I+RAK  L   +   GAYS+S+GI  V + VA+FIERRDG+ SDP  IFLTDGAS GV + L  +I+   DG+L+P+PQYPLYSATI L  GS + Y L E   W L+++ L+ S   A SKGI  RA+VIINP
Sbjct:   52 KSMTIDNICQNVRNAQYAVRGELVIRAEAISHQLQKQKTEGTKTLPFEEIVYCNIGNPQQLKQKPLTYFRQVVSLVECPDLLDNPYVEKIYPADVISRAKEILGSINNTTGAYSNSQGIGLVLRSVADFIERRDGHKSDPSEIFLTDGASVGVQRILKLLIKDRSDGILIPIPQYPLYSATIELYNGSQLGYLLNEEKGWSLEISQLEHSYNDAVSKGINPRALVIINP 280          
BLAST of CF834419 vs. ExPASy Swiss-Prot
Match: ALAT2_DANRE (Alanine aminotransferase 2-like OS=Danio rerio GN=gpt2l PE=2 SV=2)

HSP 1 Score: 191.43 bits (485), Expect = 4.871e-48
Identity = 111/229 (48.47%), Postives = 149/229 (65.07%), Query Frame = 1
Query:  154 KALDYESMNENVKKVQYAVRGELYLRASELQKEGKK--------IIFTNVGNPHALGQRPLTFPRQVVALCQAPFLLDDPNVGIVFPADAIARAKHYLSLTSGG-LGAYSDSRGIPGVRKEVAEFIERRDG-YPSDPELIFLTDGASKGVMQTLNCVIRGEG---DGVLVPVPQYPLYSATISLLGGSLVPYYLEETANWGLDLNDLQQSVAQARSKGITVRAMVIINP 801
            K L  ++MN NVKKV YAVRG +  RA +++KE K+        +I  N+G+ HA+GQRP+TF RQV+ALC  P LLDD      FP DA  RA+  L    G  +GAY+ S+GI  VR++VA++IERRDG  PSDP+ I+LT GAS G++  L  +  GEG    GV++ +PQYPLYSA+I+ LG   + YYL E   W LD+++LQ+S+ QA  K    R + IINP
Sbjct:   72 KVLTVDTMNANVKKVDYAVRGPIVQRAVQIEKELKEGVKKPFDEVIKANIGDAHAMGQRPITFFRQVMALCTYPQLLDDNK----FPEDAKNRARRILQSCGGNSIGAYTTSQGIDCVRQDVAKYIERRDGGIPSDPDNIYLTTGASDGIVTILKLLTAGEGLTRTGVMISIPQYPLYSASIAELGAVQINYYLNEEKCWSLDISELQRSL-QAARKHCNPRVLCIINP 295          
BLAST of CF834419 vs. ExPASy Swiss-Prot
Match: ALAT_SCHPO (Putative alanine aminotransferase OS=Schizosaccharomyces pombe GN=SPBC582.08 PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 2.418e-47
Identity = 102/225 (45.33%), Postives = 135/225 (60.00%), Query Frame = 1
Query:  163 DYESMNENVKKVQYAVRGELYLRASELQKE---------GKKIIFTNVGNPHALGQRPLTFPRQVVALCQAPFLLDDPN---VGIVFPADAIARAKHYLSLTSGGLGAYSDSRGIPGVRKEVAEFIERRDGYPSDPELIFLTDGASKGVMQTLNCVIRGEGDGVLVPVPQYPLYSATISLLGGSLVPYYLEETANWGLDLNDLQQSVAQARSKGITVRAMVIINP 801
            D  S+N+ V K  YAVRG L + A E+Q +           +I++ N+GNP  +GQ P+TF RQV++LCQ P LLD         +FP D + R+K  L   SG LGAYS S+GIP VR+ VA+FI  RDG+  +P  I+LT GAS      +  +I    DGV+VP PQYPLY A I L+ GS+V Y L E  NW +D +  ++S  +A  KGI VR  V+INP
Sbjct:   29 DLNSLNQQVFKANYAVRGALAILADEIQDDLLENPSSYPFSEIVYANIGNPQQMGQSPITFVRQVLSLCQYPTLLDHAEEKWFQNLFPTDVVQRSKMLLK-ESGSLGAYSASQGIPLVRRHVADFIRARDGFDCEPSDIYLTSGASHAARLIMTLIIARPTDGVMVPAPQYPLYGAQIDLMSGSMVSYSLSEENNWDIDFDQFKKSFDEASKKGINVRLCVVINP 252          
BLAST of CF834419 vs. ExPASy Swiss-Prot
Match: ALAT1_HUMAN (Alanine aminotransferase 1 OS=Homo sapiens GN=GPT PE=1 SV=3)

HSP 1 Score: 184.496 bits (467), Expect = 5.955e-46
Identity = 106/229 (46.29%), Postives = 145/229 (63.32%), Query Frame = 1
Query:  154 KALDYESMNENVKKVQYAVRGELYLRASELQKEGKK--------IIFTNVGNPHALGQRPLTFPRQVVALCQAPFLLDDPNVGIVFPADAIARAKHYLSLTSG-GLGAYSDSRGIPGVRKEVAEFIERRDG-YPSDPELIFLTDGASKGVMQTLNCVIRGEGD---GVLVPVPQYPLYSATISLLGGSLVPYYLEETANWGLDLNDLQQSVAQARSKGITVRAMVIINP 801
            K L  + MN  V++V+YAVRG +  RA EL++E ++        +I  N+G+  A+GQRP+TF RQV+ALC  P LL  PN    FP DA  RA+  L    G  LGAYS S GI  +R++VA +IERRDG  P+DP  +FL+ GAS  ++  L  ++ GEG    GVL+P+PQYPLYSAT++ LG   V YYL+E   W LD+ +L +++ QAR      RA+ +INP
Sbjct:   19 KVLTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFLRQVLALCVNPDLLSSPN----FPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDVARYIERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAWALDVAELHRALGQARDH-CRPRALCVINP 242          
BLAST of CF834419 vs. ExPASy Swiss-Prot
Match: ALAT2_MOUSE (Alanine aminotransferase 2 OS=Mus musculus GN=Gpt2 PE=2 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 6.584e-45
Identity = 101/228 (44.30%), Postives = 146/228 (64.04%), Query Frame = 1
Query:  154 KALDYESMNENVKKVQYAVRGELYLRASELQKEGKK--------IIFTNVGNPHALGQRPLTFPRQVVALCQAPFLLDDPNVGIVFPADAIARAKHYLSLTSGG-LGAYSDSRGIPGVRKEVAEFIERRDGYPSDPELIFLTDGASKGVMQTLNCVIRGEG---DGVLVPVPQYPLYSATISLLGGSLVPYYLEETANWGLDLNDLQQSVAQARSKGITVRAMVIINP 801
            + L  ESMN  VK V+YAVRG + L+A E++ E ++        +I  N+G+ HA+GQ+P+TF RQV+ALC  P LL+ P+    FP DA  RA+  L    G  LG+YS S+G+  +R++VA FI RRDG P+DP+ I+LT GAS G+   L  ++ G G    GV++P+PQYPLYSA IS L    V YYL+E   W L++++L++++ QA+      + + IINP
Sbjct:   46 RILTLESMNPQVKAVEYAVRGPIVLKAGEIEMELQRGIKKPFTEVIRANIGDAHAMGQQPITFLRQVMALCTYPNLLNSPS----FPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAFITRRDGVPADPDNIYLTTGASDGISTILKLLVSGGGKSRTGVMIPIPQYPLYSAVISELDAVQVNYYLDEENCWALNVDELRRALRQAKDH-CDPKVLCIINP 268          
BLAST of CF834419 vs. ExPASy Swiss-Prot
Match: ALAT1_RAT (Alanine aminotransferase 1 OS=Rattus norvegicus GN=Gpt PE=1 SV=2)

HSP 1 Score: 179.104 bits (453), Expect = 2.502e-44
Identity = 103/229 (44.98%), Postives = 146/229 (63.76%), Query Frame = 1
Query:  154 KALDYESMNENVKKVQYAVRGELYLRASELQKEGKK--------IIFTNVGNPHALGQRPLTFPRQVVALCQAPFLLDDPNVGIVFPADAIARAKHYLSLTSG-GLGAYSDSRGIPGVRKEVAEFIERRDG-YPSDPELIFLTDGASKGVMQTLNCVIRGEG---DGVLVPVPQYPLYSATISLLGGSLVPYYLEETANWGLDLNDLQQSVAQARSKGITVRAMVIINP 801
            K L  ++MN  V++V+YAVRG +  RA EL++E ++        +I  N+G+  A+GQRP+TF RQV+ALC  P LL  P+    FP DA  RA+  L    G  LGAYS S GI  +R++VA++IERRDG  P+DP  IFL+ GAS  ++  L  ++ GEG    GVL+P+PQYPLYSA ++ L    V YYL+E   W LD+ +L++++ QAR +    R + +INP
Sbjct:   19 KVLTLDTMNPCVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFFRQVLALCVYPNLLSSPD----FPEDAKRRAERILQACGGHSLGAYSISSGIQPIREDVAQYIERRDGGIPADPNNIFLSTGASDAIVTMLKLLVSGEGRARTGVLIPIPQYPLYSAALAELDAVQVDYYLDEERAWALDIAELRRALCQARDR-CCPRVLCVINP 242          
BLAST of CF834419 vs. ExPASy Swiss-Prot
Match: ALAT1_MOUSE (Alanine aminotransferase 1 OS=Mus musculus GN=Gpt PE=2 SV=3)

HSP 1 Score: 179.104 bits (453), Expect = 2.502e-44
Identity = 103/229 (44.98%), Postives = 146/229 (63.76%), Query Frame = 1
Query:  154 KALDYESMNENVKKVQYAVRGELYLRASELQKEGKK--------IIFTNVGNPHALGQRPLTFPRQVVALCQAPFLLDDPNVGIVFPADAIARAKHYLSLTSG-GLGAYSDSRGIPGVRKEVAEFIERRDG-YPSDPELIFLTDGASKGVMQTLNCVIRGEG---DGVLVPVPQYPLYSATISLLGGSLVPYYLEETANWGLDLNDLQQSVAQARSKGITVRAMVIINP 801
            K L  ++MN  V++V+YAVRG +  RA EL++E ++        +I  N+G+  A+GQRP+TF RQV+ALC  P LL  P+    FP DA  RA+  L    G  LGAYS S GI  +R++VA++IERRDG  P+DP  IFL+ GAS  ++  L  ++ GEG    GVL+P+PQYPLYSA ++ L    V YYL+E   W LD+ +L++++ QAR +    R + +INP
Sbjct:   19 KVLTLDTMNPCVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFFRQVLALCVYPNLLSSPD----FPEDAKRRAERILQACGGHSLGAYSISSGIQPIREDVAQYIERRDGGIPADPNNIFLSTGASDAIVTMLKLLVAGEGRARTGVLIPIPQYPLYSAALAELDAVQVDYYLDEERAWALDIAELRRALCQARDR-CCPRVLCVINP 242          
BLAST of CF834419 vs. ExPASy Swiss-Prot
Match: ALAT1_BOVIN (Alanine aminotransferase 1 OS=Bos taurus GN=GPT PE=1 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 2.502e-44
Identity = 103/229 (44.98%), Postives = 145/229 (63.32%), Query Frame = 1
Query:  154 KALDYESMNENVKKVQYAVRGELYLRASELQKEGKK--------IIFTNVGNPHALGQRPLTFPRQVVALCQAPFLLDDPNVGIVFPADAIARAKHYLSLTSG-GLGAYSDSRGIPGVRKEVAEFIERRDG-YPSDPELIFLTDGASKGVMQTLNCVIRGEG---DGVLVPVPQYPLYSATISLLGGSLVPYYLEETANWGLDLNDLQQSVAQARSKGITVRAMVIINP 801
            K L  +SMN  V++V+YAVRG +  RA EL++E ++        +I  N+G+  A+GQ P+TFPRQV+ALC  P LL+ P+    FP DA  RA+  L    G  LGAYS S G+  +R++VA +IERRDG  P+DP  IFL+ GAS  ++  L  ++ GEG    GVL+P+PQYPLYSA ++      V YYL+E   W LD+ +L++++ QAR      RA+ +INP
Sbjct:   19 KVLTLDSMNPYVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQIPITFPRQVLALCVHPDLLNSPD----FPDDAKRRAERILQACGGHSLGAYSISAGVQMIREDVARYIERRDGGIPADPNNIFLSTGASDAIVTVLKLLVTGEGRTRTGVLIPIPQYPLYSAALAEFNAVQVDYYLDEERAWALDVAELRRALRQARDH-CRPRALCVINP 242          
The following BLAST results are available for this feature:
BLAST of CF834419 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 21
Match NameE-valueIdentityDescription
ALA2_PANMI2.849e-5650.23Alanine aminotransferase 2 OS=Panicum miliaceum PE... [more]
ALA2_HORVU6.347e-5649.32Alanine aminotransferase 2 OS=Hordeum vulgare PE=2... [more]
ALAM_DICDI4.114e-5550.66Probable alanine aminotransferase, mitochondrial O... [more]
ALAT2_DANRE4.871e-4848.47Alanine aminotransferase 2-like OS=Danio rerio GN=... [more]
ALAT_SCHPO2.418e-4745.33Putative alanine aminotransferase OS=Schizosacchar... [more]
ALAT1_HUMAN5.955e-4646.29Alanine aminotransferase 1 OS=Homo sapiens GN=GPT ... [more]
ALAT2_MOUSE6.584e-4544.30Alanine aminotransferase 2 OS=Mus musculus GN=Gpt2... [more]
ALAT1_RAT2.502e-4444.98Alanine aminotransferase 1 OS=Rattus norvegicus GN... [more]
ALAT1_MOUSE2.502e-4444.98Alanine aminotransferase 1 OS=Mus musculus GN=Gpt ... [more]
ALAT1_BOVIN2.502e-4444.98Alanine aminotransferase 1 OS=Bos taurus GN=GPT PE... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_05H24_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa05H24, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834419 ID=CF834419; Name=CF834419; organism=Citrus sinensis; type=EST; length=803bp
CCTTTTGATCAAATCCTAATATCAGCTTAGCAGGCGCTTGCATTTTTCTG
TGCACGTCAGTGTAATTGTAAACATTCAATTGGCTCGAGCCGAAGTTAGC
CCACAAGCACTCTCTCAGTTAAGATCCCAGGCGTTTAGTACATCATGGCA
CCAAAGGCATTGGACTATGAGTCTATGAATGAAAACGTGAAGAAGGTTCA
ATATGCTGTTAGAGGAGAGTTGTATCTTCGAGCTTCTGAGCTTCAGAAGG
AAGGGAAGAAGATTATTTTTACAAATGTTGGCAACCCTCATGCTCTAGGA
CAGAGGCCATTGACCTTCCCTCGGCAGGTGGTTGCTCTTTGCCAGGCTCC
ATTTTTGCTAGATGATCCTAATGTAGGAATTGTCTTCCCTGCTGATGCAA
TTGCAAGAGCTAAACATTATCTTTCCCTGACTTCAGGGGGTCTAGGTGCT
TACAGTGACTCCAGAGGTATTCCAGGTGTAAGGAAGGAAGTAGCAGAGTT
CATTGAAAGGCGCGATGGTTATCCAAGTGACCCAGAACTCATATTTCTCA
CTGATGGTGCCAGCAAAGGAGTTATGCAGACTTTAAATTGTGTCATCCGT
GGTGAAGGGGATGGGGTTTTGGTTCCAGTTCCACAGTACCCACTGTACTC
AGCTACCATATCTCTTTTAGGTGGTTCTCTTGTTCCATACTACTTGGAAG
AGACAGCAAATTGGGGTCTTGATCTTAATGACCTTCAGCAGTCAGTTGCA
CAGGCTCGTTCTAAAGGAATTACGGTTAGAGCAATGGTGATTATTAATCC
TGG
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