CF835137

Overview
NameCF835137
Unique NameCF835137
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length696
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF835137 vs. ExPASy Swiss-Prot
Match: C76B1_HELTU (7-ethoxycoumarin O-deethylase OS=Helianthus tuberosus GN=CYP76B1 PE=1 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 1.829e-50
Identity = 102/216 (47.22%), Postives = 151/216 (69.91%), Query Frame = 2
Query:   56 LVFTLVWVMALSFVSRGRHKQLPPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGQVTTVVVSSPSMAKAILQNHDLSFCDRMVPEAILSLPFPHHEFSLAWLPVSTMWKNLRKISNMHIFTSQKLDANQELRRKKIKDLLAHVEDNCRAGKAIDVGQAAFNTSLNLLSNTIFSIDLVNPNE---REFKDTVSGILEEAGKPNLSDHFP 694
            L + L+WV+ +     G+ K LPPGP   P+IGNL  LGA PH+SLAKLAKIHGPIMSL+LGQ+TT+V+SS + A+ +L+  DL+F  R VP+A+ +  + H   S+++L V T W+ LR+I + +IF++  L+A Q LR KK+++L+A+      + + + +G+AAF TSLNLLSNTIFS DL +P E   +EF++ ++ I+ ++ K NL D FP
Sbjct:   12 LSYILIWVLGV-----GKPKNLPPGPTRLPIIGNLHLLGALPHQSLAKLAKIHGPIMSLQLGQITTLVISSATAAEEVLKKQDLAFSTRNVPDAVRA--YNHERHSISFLHVCTEWRTLRRIVSSNIFSNSSLEAKQHLRSKKVEELIAYCRKAALSNENVHIGRAAFRTSLNLLSNTIFSKDLTDPYEDSGKEFREVITNIMVDSAKTNLVDVFP 220          
BLAST of CF835137 vs. ExPASy Swiss-Prot
Match: C76C4_ARATH (Cytochrome P450 76C4 OS=Arabidopsis thaliana GN=CYP76C4 PE=2 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 4.515e-41
Identity = 87/213 (40.85%), Postives = 139/213 (65.26%), Query Frame = 2
Query:   68 LVWVMALSFVSRGRHKQLPPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGQVTTVVVSSPSMAKAILQNHDLSFCDRMVPEAILSLPFPHHEFSLAWLPVST-MWKNLRKISNMHIFTSQKLDANQELRRKKIKDLLAHVEDNCRAGKAIDVGQAAFNTSLNLLSNTIFSIDLVNPNER---EFKDTVSGILEEAGKPNLSDHFP 694
            L+   A S  S GR   LPPGP   P+IGN+ ++G  PH S A LAKI+GPIMSL+ G + +VV++SP  A+ +L+ HD     R   ++I    F H E S+ WLP S+  W+ LRK+S   +F+ Q+ +A + LR KK+++L++ + ++    +A+D+ +A++ T LN++SN +FS+DL + + +   EF+DTV G +E AGKP+ +++FP
Sbjct:   21 LISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRC--FGHEEVSVIWLPPSSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFP 231          
BLAST of CF835137 vs. ExPASy Swiss-Prot
Match: C76C3_ARATH (Cytochrome P450 76C3 OS=Arabidopsis thaliana GN=CYP76C3 PE=2 SV=2)

HSP 1 Score: 166.777 bits (421), Expect = 1.006e-40
Identity = 88/221 (39.82%), Postives = 134/221 (60.63%), Query Frame = 2
Query:   53 PLVFTLVWVMALSFVSRGRHKQ----LPPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGQVTTVVVSSPSMAKAILQNHDLSFCDRMVPEAILSLPFPHHEFSLAWLPVSTMWKNLRKISNMHIFTSQKLDANQELRRKKIKDLLAHVEDNCRAGKAIDVGQAAFNTSLNLLSNTIFSIDLV----NPNEREFKDTVSGILEEAGKPNLSDHF 691
            PL F L         ++ R       LPPGP   P++GN+ +LG  PH+SLA  +K +GPIMSL+LG++T VV+SSP  AK  L+ HD     R   +A+ +  F HH+ S+ W+P S  W+ L+K    ++ + Q LDA Q LR +K+++L++ V +    G+AID+ +A+F TS N++SN +FS+DL     N +  EF +TV  + + AG PN+ D+F
Sbjct:   12 PLYFLLTLFFFFFATAKTRRSSSTGTLPPGPPILPLVGNIFQLGFNPHRSLAAFSKTYGPIMSLKLGRLTAVVISSPEAAKEALRTHDHVMSARTFNDALRA--FDHHKHSIVWIPPSARWRFLKKTITKYLLSPQNLDAIQSLRMRKVEELVSLVNEFRERGEAIDLARASFVTSFNIISNALFSVDLATYDSNSSSYEFHNTVVHLTDIAGIPNVGDYF 230          
BLAST of CF835137 vs. ExPASy Swiss-Prot
Match: C76C2_ARATH (Cytochrome P450 76C2 OS=Arabidopsis thaliana GN=CYP76C2 PE=2 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 2.319e-37
Identity = 77/195 (39.49%), Postives = 126/195 (64.62%), Query Frame = 2
Query:  122 PPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGQVTTVVVSSPSMAKAILQNHDLSFCDRMVPEAILSLPFPHHEFSLAWL-PVSTMWKNLRKISNMHIFTSQKLDANQELRRKKIKDLLAHVEDNCRAGKAIDVGQAAFNTSLNLLSNTIFSIDLVNPNERE---FKDTVSGILEEAGKPNLSDHFP 694
            PPGP   P+IGN+  +G  PH S A L+K +GPIMSL+ G + TVVV+SP  A+ +L+ +D     R    +I S+   H + S+ WL P S+ W+ LRK+S   +F+ Q+++A + LR  K+K+L++ + ++    +A+D+ +A F T+LN++SN +FS+DL N +  +   F+DTV G++E  G P+ ++ FP
Sbjct:   39 PPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSRTPTNSIRSI--NHDKVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFP 231          
BLAST of CF835137 vs. ExPASy Swiss-Prot
Match: C76C1_ARATH (Cytochrome P450 76C1 OS=Arabidopsis thaliana GN=CYP76C1 PE=2 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 3.029e-37
Identity = 81/220 (36.82%), Postives = 138/220 (62.73%), Query Frame = 2
Query:   50 CPLVFTLVWVMALSFVSRGRHKQLPPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGQVTTVVVSSPSMAKAILQNHDLSFCDRMVPEAILSLPFPHHEFSLAWLPVST-MWKNLRKISNMHIFTSQKLDANQELRRKKIKDLLAHVEDNCRAGKAIDVGQAAFNTSLNLLSNTIFSIDLVNPNERE----FKDTVSGILEEAGKPNLSDHFP 694
            C L+FT         +SRG    LPPGP   P+IGN+  +G  PH+S A+L+K +GP+MSL+LG + TVV++SP  A+ +L+ HD     R    A+ S+   H + SL WLP S+  W+ LR++S   + + Q+++A + LR  K+K+L++ + ++    +++D+ + AF T+LN++SN +FS+DL + N +      +DTV  +++ AG P+ +++FP
Sbjct:   19 CFLIFTTT---RSGRISRGA-TALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAVRSI--NHQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFP 232          
BLAST of CF835137 vs. ExPASy Swiss-Prot
Match: C80B2_ESCCA ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 1.901e-31
Identity = 74/211 (35.07%), Postives = 120/211 (56.87%), Query Frame = 2
Query:   77 VMALSFVSRGRHKQLPPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGQVTTVVVSSPSMAKAILQNHDLSFCDRMVPEAILSLPFPHHEFSLAWLPVSTMWKNLRKISNMHIFTSQKLDANQELRRKKIKDLLAHVEDNCRAGKAIDVGQAAFNTSLNLLSNTIFSIDLV-----NPNEREFKDTVSGILEEAGKPNLSDHFP 694
            ++ L F S G HK LPPGP+P+P++GNLL+LG KPH   A+LA+ +G I +L++G  T VV S+ S A  IL+ HD     R V ++       H E S+ W   +  WKNLRK+    +FT + +++   +R KK ++++ ++    + G+ + + +  F T +N+  N IFS ++      N    EFK+ +  +LE     N +D+FP
Sbjct:   16 ILYLLFGSSG-HKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVK--GHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMK--KQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFP 221          
BLAST of CF835137 vs. ExPASy Swiss-Prot
Match: C80B1_ESCCA ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica GN=CYP80B1 PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 3.243e-31
Identity = 75/211 (35.55%), Postives = 121/211 (57.35%), Query Frame = 2
Query:   77 VMALSFVSRGRHKQLPPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGQVTTVVVSSPSMAKAILQNHDLSFCDRMVPEAILSLPFPHHEFSLAWLPVSTMWKNLRKISNMHIFTSQKLDANQELRRKKIKDLLAHVEDNCRAGKAIDVGQAAFNTSLNLLSNTIFS---IDLVNPN--EREFKDTVSGILEEAGKPNLSDHFP 694
            ++ L F   G HK LPPGP+P+P++GNLL+LG KPH   A+LA+ +G I +L++G  T VV S+ S A  IL+ HD     R V ++       H E S+ W   +  WKNLRK+    +FT + +++   +R KK ++++ ++    + G+ + + +  F T +N+  N IFS    +L +PN    EFK+ +  +LE     N +D+FP
Sbjct:   15 ILYLLFGGSG-HKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVK--GHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMK--KQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFP 220          
BLAST of CF835137 vs. ExPASy Swiss-Prot
Match: C71BC_ARATH (Cytochrome P450 71B12 OS=Arabidopsis thaliana GN=CYP71B12 PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 2.841e-27
Identity = 63/217 (29.03%), Postives = 118/217 (54.38%), Query Frame = 2
Query:   47 YCPLVFTLVWVMALSFVSRGRHKQLPPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGQVTTVVVSSPSMAKAILQNHDLSFCDRMVPEAILSLPFPHHEFSLAWLPVSTMWKNLRKISNMHIFTSQKLDANQELRRKKIKDLLAHVEDNCRAGKAIDVGQAAFNTSLNLLSNTIFSIDLVNPN-EREFKDTVSGILEEAGKPNLSDHFP 694
            Y  + F     M +  + R   K LPPGP   P+IGNL +LG+KPH+S+ KL++ +GP+MSL+ G V+TVV S+P   K +L+  D+  C R  P         ++   L + P S  W+ +RK++ + ++T++++ + Q  R++++  L+  ++      K +++ +     S +++    F I+L     E  +++ + G +E  G    +D+FP
Sbjct:    5 YIIVAFVFFSSMIIVRIIRKTKKNLPPGPPRLPIIGNLHQLGSKPHRSMFKLSETYGPLMSLKFGSVSTVVASTPETVKEVLKTFDVECCSR--PNMTYPARVTYNLKDLCFSPYSKYWREVRKMTVVELYTAKRVQSFQHTRKEEVAALVDFIKQAASLEKPVNLNKKLMKLSGSVICRVAFGINLQGSKLENTYEEVIQGTVELVGSFAAADYFP 219          
BLAST of CF835137 vs. ExPASy Swiss-Prot
Match: C76A2_SOLME (Cytochrome P450 76A2 OS=Solanum melongena GN=CYP76A2 PE=2 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 8.267e-27
Identity = 65/199 (32.66%), Postives = 113/199 (56.78%), Query Frame = 2
Query:  116 QLPPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGQVTTVVVSSPSMAKAILQNHDLSFCDRMVPEAILSLPFPHHEFSLAWLPVSTMWKNLRKISNMHIFTSQKLDANQELRRKKIKDLLAHVE---DNCRAGKAIDVGQAAFNTSLNLLSNTIFS---IDLVNPNEREFKDTVSGILEEAGKPNLSDHFP 694
            + PPGP   P+ GN+ ELG +P+K +A L + +GP++ L+LG   T+VV +   ++ + +NHD+SF +R++P+  ++    +++ SLA  P    W+  R+I  + +F  +K+   + +RRK + ++L  +E   ++   G  I+V +  F  S N+L N I S    DL +    EF   +  I E +G  N+SD FP
Sbjct:   34 KFPPGPPGLPIFGNMFELGTEPYKKMAVLRQKYGPVLWLKLGSTYTMVVQTAQASEELFKNHDISFANRVIPD--VNQAHSYYQGSLAIAPYGPFWRFQRRICTIEMFVHKKISETEPVRRKCVDNMLKWIEKEANSAEKGSGIEVTRFVFLASFNMLGNLILSKDLADLESEEASEFFIAMKRINEWSGIANVSDIFP 230          
BLAST of CF835137 vs. ExPASy Swiss-Prot
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 2.405e-26
Identity = 69/225 (30.67%), Postives = 121/225 (53.78%), Query Frame = 2
Query:   56 LVFTLVWVMALSFVSRGRHKQ-----LPPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGQVTTVVVSSPSMAKAILQNHDLSFCDRMVPEAILSLPFPHHEFSLAWLPVSTMWKNLRKISNMHIFTSQKLDANQELRRKKIKDLLAHVEDNCRAGKAIDVGQAAFNTSLNLLSNTIFSIDL-------VNPNEREFKDTVSGILEEAGKPNLSDHFP 694
            +++T+++   L F+ R   ++     LPPGP+P+P+IGNL+ LG KPH+S A +A+ +GP+M L++G V  VV +S S+A   L+ HD +F  R        + + + +  L + P    W+ LRKI ++H+F+++ LD  + +R+ ++K L   +       K + +GQ     + N L+  +    +       V+P   EFK  V  ++  AG  N+ D  P
Sbjct:    7 ILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAMAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQD--LVFAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALAS--AGQKPVKLGQLLNVCTTNALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIP 227          
The following BLAST results are available for this feature:
BLAST of CF835137 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 118
Match NameE-valueIdentityDescription
C76B1_HELTU1.829e-5047.227-ethoxycoumarin O-deethylase OS=Helianthus tubero... [more]
C76C4_ARATH4.515e-4140.85Cytochrome P450 76C4 OS=Arabidopsis thaliana GN=CY... [more]
C76C3_ARATH1.006e-4039.82Cytochrome P450 76C3 OS=Arabidopsis thaliana GN=CY... [more]
C76C2_ARATH2.319e-3739.49Cytochrome P450 76C2 OS=Arabidopsis thaliana GN=CY... [more]
C76C1_ARATH3.029e-3736.82Cytochrome P450 76C1 OS=Arabidopsis thaliana GN=CY... [more]
C80B2_ESCCA1.901e-3135.07(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS... [more]
C80B1_ESCCA3.243e-3135.55(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (F... [more]
C71BC_ARATH2.841e-2729.03Cytochrome P450 71B12 OS=Arabidopsis thaliana GN=C... [more]
C76A2_SOLME8.267e-2732.66Cytochrome P450 76A2 OS=Solanum melongena GN=CYP76... [more]
F3PH_PETHY2.405e-2630.67Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=C... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS02_06L18_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06L18, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835137 ID=CF835137; Name=CF835137; organism=Citrus sinensis; type=EST; length=696bp
CAAAATATCAACTTTTATTCAAAGATGGATTTGTTAATTAACTGCATACT
GTCCGCTTGTATTCACATTGGTTTGGGTGATGGCTTTAAGTTTCGTTTCC
AGAGGACGACACAAACAGCTTCCACCGGGACCGAGGCCTTATCCAGTGAT
CGGAAACCTTCTGGAGCTAGGTGCTAAACCTCACAAGTCACTGGCCAAGC
TTGCCAAGATTCATGGGCCCATAATGAGTCTAAGACTTGGCCAGGTGACC
ACAGTAGTGGTTTCTTCTCCAAGCATGGCCAAAGCCATCCTTCAAAATCA
TGATTTATCATTCTGTGATCGAATGGTCCCTGAAGCAATCTTGTCACTGC
CCTTCCCGCACCATGAATTCAGTTTGGCCTGGCTTCCAGTTTCAACAATG
TGGAAAAATCTTCGAAAAATAAGCAACATGCATATTTTCACTAGTCAGAA
ACTCGATGCCAATCAAGAGCTCCGGCGCAAGAAAATCAAAGACCTTCTTG
CTCATGTTGAAGACAATTGCCGTGCGGGTAAAGCAATAGATGTTGGCCAA
GCTGCTTTCAATACTTCACTTAATTTATTATCCAACACAATTTTTTCTAT
TGATTTGGTTAATCCCAATGAGCGAGAGTTTAAGGATACAGTTTCTGGTA
TCTTGGAAGAAGCAGGAAAGCCTAACTTGTCCGACCATTTTCCTCT
back to top