CK933305

Overview
NameCK933305
Unique NameCK933305
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length859
Libraries
Library NameType
Developing fruit juice sac at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK933305 vs. ExPASy Swiss-Prot
Match: CAMT_POPTM (Caffeoyl-CoA O-methyltransferase OS=Populus tremuloides PE=2 SV=1)

HSP 1 Score: 461.07 bits (1185), Expect = 3.671e-129
Identity = 221/247 (89.47%), Postives = 239/247 (96.76%), Query Frame = 2
Query:   65 MASNAEDQQNQQGGHKEVGHKSLLQSDALYQYILETSVYPREAESMKELRELTAKHPWNVMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVAHKIDFREGPALPVLDLLIQDEKNHGSFDFIFVDADKDNYLNYHKRLVELVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGVTICRRIK 805
            MA+N E+QQ+Q G H+EVGHKSLLQSDALYQYILETSVYPRE E MKELRE+TAKHPWN+MTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALA+P+DGKILAMDINRENYELGLPVIQKAGVAHKIDF+EGPALPVLD +I+D K HGSFDFIFVDADKDNY+NYHKRL+ELVKVGG+IGYDNTLWNGSVVAPPDAP+RKYVRYYRDFVLELNKALAADPRIEICMLPVGDG+T+CRRI+
Sbjct:    1 MATNGEEQQSQAGRHQEVGHKSLLQSDALYQYILETSVYPREPECMKELREVTAKHPWNIMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINRENYELGLPVIQKAGVAHKIDFKEGPALPVLDQMIEDGKYHGSFDFIFVDADKDNYINYHKRLIELVKVGGLIGYDNTLWNGSVVAPPDAPMRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGITLCRRIQ 247          
BLAST of CK933305 vs. ExPASy Swiss-Prot
Match: CAMT1_POPTR (Caffeoyl-CoA O-methyltransferase 1 OS=Populus trichocarpa GN=CCOAOMT1 PE=2 SV=1)

HSP 1 Score: 460.685 bits (1184), Expect = 4.794e-129
Identity = 221/247 (89.47%), Postives = 239/247 (96.76%), Query Frame = 2
Query:   65 MASNAEDQQNQQGGHKEVGHKSLLQSDALYQYILETSVYPREAESMKELRELTAKHPWNVMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVAHKIDFREGPALPVLDLLIQDEKNHGSFDFIFVDADKDNYLNYHKRLVELVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGVTICRRIK 805
            MA+N E+QQ+Q G H+EVGHKSLLQSDALYQYILETSVYPRE E MKELRE+TAKHPWN+MTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALA+P+DGKILAMDINRENYELGLPVIQKAGVAHKIDF+EGPALPVLD +I+D K HGSFDFIFVDADKDNY+NYHKRL+ELVKVGG+IGYDNTLWNGSVVAPPDAP+RKYVRYYRDFVLELNKALAADPRIEICMLPVGDG+T+CRRI+
Sbjct:    1 MATNGEEQQSQAGRHQEVGHKSLLQSDALYQYILETSVYPREPECMKELREVTAKHPWNIMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINRENYELGLPVIQKAGVAHKIDFKEGPALPVLDQMIEDGKCHGSFDFIFVDADKDNYINYHKRLIELVKVGGLIGYDNTLWNGSVVAPPDAPMRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGITLCRRIQ 247          
BLAST of CK933305 vs. ExPASy Swiss-Prot
Match: CAMT2_EUCGL (Caffeoyl-CoA O-methyltransferase 2 OS=Eucalyptus globulus GN=CCOAOMT2 PE=2 SV=1)

HSP 1 Score: 456.062 bits (1172), Expect = 1.181e-127
Identity = 216/246 (87.80%), Postives = 236/246 (95.93%), Query Frame = 2
Query:   65 MASNAEDQQNQQGGHKEVGHKSLLQSDALYQYILETSVYPREAESMKELRELTAKHPWNVMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVAHKIDFREGPALPVLDLLIQDEKNHGSFDFIFVDADKDNYLNYHKRLVELVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGVTICRRI 802
            MA+NAE QQ Q   H EVGHKSLLQSDALYQYILETSVYPRE ESMKELRE+TAKHPWN+MTTSADEGQFLNMLLKL+NAKNTMEIGVYTGYSLLATALALPDDGKILAMDINREN+E+GLPVI+KAG+AHKIDFREGPALP+LD L+QDEKNHG++DFIFVDADKDNY+NYHKRL++LVKVGG+IGYDNTLWNGSVVAP DAPLRKYVRYYRDFVLELNKALA DPR+EICMLPVGDG+T+CRR+
Sbjct:    1 MAANAEPQQTQPAKHSEVGHKSLLQSDALYQYILETSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNMLLKLINAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENFEIGLPVIEKAGLAHKIDFREGPALPLLDQLVQDEKNHGTYDFIFVDADKDNYINYHKRLIDLVKVGGLIGYDNTLWNGSVVAPADAPLRKYVRYYRDFVLELNKALAVDPRVEICMLPVGDGITLCRRV 246          
BLAST of CK933305 vs. ExPASy Swiss-Prot
Match: CAMT_MEDSA (Caffeoyl-CoA O-methyltransferase OS=Medicago sativa GN=CCOMT PE=1 SV=1)

HSP 1 Score: 454.907 bits (1169), Expect = 2.631e-127
Identity = 217/247 (87.85%), Postives = 238/247 (96.36%), Query Frame = 2
Query:   65 MASNAEDQQNQQGGHKEVGHKSLLQSDALYQYILETSVYPREAESMKELRELTAKHPWNVMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVAHKIDFREGPALPVLDLLIQDEKNHGSFDFIFVDADKDNYLNYHKRLVELVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGVTICRRIK 805
            MA+N + +Q + G H+EVGHKSLLQSDALYQYILETSV+PRE E+MKELRE+TAKHPWN+MTTSADEGQFL+MLLKL+NAKNTMEIGVYTGYSLLATALA+P+DGKILAMDIN+ENYELGLPVI+KAGV HKIDFREGPALPVLD +I+DEKNHGS+DFIFVDADKDNYLNYHKRL++LVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALA DPRIEICMLPVGDG+TICRRIK
Sbjct:    1 MATNEDQKQTESGRHQEVGHKSLLQSDALYQYILETSVFPREHEAMKELREVTAKHPWNIMTTSADEGQFLSMLLKLINAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINKENYELGLPVIKKAGVDHKIDFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAVDPRIEICMLPVGDGITICRRIK 247          
BLAST of CK933305 vs. ExPASy Swiss-Prot
Match: CAMT6_TOBAC (Caffeoyl-CoA O-methyltransferase 6 OS=Nicotiana tabacum GN=CCOAOMT6 PE=2 SV=1)

HSP 1 Score: 453.366 bits (1165), Expect = 7.654e-127
Identity = 218/246 (88.62%), Postives = 235/246 (95.53%), Query Frame = 2
Query:   65 MASNAEDQQNQQGGHKEVGHKSLLQSDALYQYILETSVYPREAESMKELRELTAKHPWNVMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVAHKIDFREGPALPVLDLLIQDEKNHGSFDFIFVDADKDNYLNYHKRLVELVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGVTICRRI 802
            MA N   Q+NQ   H+EVGHKSLLQSDALYQYILETSVYPRE E MKELRELTAKHPWN+MTTSADEGQFL+MLLKL+ AKNTMEIGVYTGYSLLATALALPDDGKILAMDIN+ENYELGLPVIQKAGVAHKIDFREGPALPVLDL+I+D+ NHG++DFIFVDADKDNY+NYHKR++ELVKVGGVIGYDNTLWNGSVVAPPDAP+RKYVRYYRDFVLELNKALAADPRIEICMLPVGDG+T+CRRI
Sbjct:    1 MAENGAAQENQVTKHQEVGHKSLLQSDALYQYILETSVYPREPEPMKELRELTAKHPWNLMTTSADEGQFLSMLLKLIIAKNTMEIGVYTGYSLLATALALPDDGKILAMDINKENYELGLPVIQKAGVAHKIDFREGPALPVLDLMIEDKNNHGTYDFIFVDADKDNYINYHKRIIELVKVGGVIGYDNTLWNGSVVAPPDAPMRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGITLCRRI 246          
BLAST of CK933305 vs. ExPASy Swiss-Prot
Match: CAMT4_ARATH (Probable caffeoyl-CoA O-methyltransferase At4g34050 OS=Arabidopsis thaliana GN=At4g34050 PE=2 SV=1)

HSP 1 Score: 451.825 bits (1161), Expect = 2.227e-126
Identity = 216/246 (87.80%), Postives = 237/246 (96.34%), Query Frame = 2
Query:   68 ASNAEDQ-QNQQGGHKEVGHKSLLQSDALYQYILETSVYPREAESMKELRELTAKHPWNVMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVAHKIDFREGPALPVLDLLIQDEKNHGSFDFIFVDADKDNYLNYHKRLVELVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGVTICRRI 802
            ++N EDQ Q+Q   H+EVGHKSLLQSD LYQYILETSVYPRE ESMKELRE+TAKHPWN+MTTSADEGQFLNML+KLVNAKNTMEIGVYTGYSLLATALALP+DGKILAMD+NRENYELGLP+I+KAGVAHKIDFREGPALPVLD ++ DEKNHG++DFIFVDADKDNY+NYHKRL++LVK+GGVIGYDNTLWNGSVVAPPDAP+RKYVRYYRDFVLELNKALAADPRIEICMLPVGDG+TICRRI
Sbjct:   13 STNGEDQKQSQNLRHQEVGHKSLLQSDDLYQYILETSVYPREPESMKELREVTAKHPWNIMTTSADEGQFLNMLIKLVNAKNTMEIGVYTGYSLLATALALPEDGKILAMDVNRENYELGLPIIEKAGVAHKIDFREGPALPVLDEIVADEKNHGTYDFIFVDADKDNYINYHKRLIDLVKIGGVIGYDNTLWNGSVVAPPDAPMRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGITICRRI 258          
BLAST of CK933305 vs. ExPASy Swiss-Prot
Match: CAMT2_POPTR (Caffeoyl-CoA O-methyltransferase 2 OS=Populus trichocarpa GN=CCOAOMT2 PE=2 SV=1)

HSP 1 Score: 451.44 bits (1160), Expect = 2.909e-126
Identity = 215/247 (87.04%), Postives = 236/247 (95.55%), Query Frame = 2
Query:   65 MASNAEDQQNQQGGHKEVGHKSLLQSDALYQYILETSVYPREAESMKELRELTAKHPWNVMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVAHKIDFREGPALPVLDLLIQDEKNHGSFDFIFVDADKDNYLNYHKRLVELVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGVTICRRIK 805
            MA+N E+QQ Q G H+EVGHKSLLQSDALYQYILETSVYPRE E MKELRELTAKHPWN+MTTSADEGQFLNMLLKL+NAKNTMEIGV+TGYSLLATALA+P+DGKILAMDINRENYELGLPVIQKAG+ HKI+F+EGPALPVLD +I+D K HG++DFIFVDADKDNY+NYHKRL+ELVKVGG+IGYDNTLWNGSVVAP DAP+RKYVRYYRDFVLELNKALAADPRIEICMLPVGDG+T+CRRIK
Sbjct:    1 MAANGEEQQTQAGRHQEVGHKSLLQSDALYQYILETSVYPREPECMKELRELTAKHPWNIMTTSADEGQFLNMLLKLINAKNTMEIGVFTGYSLLATALAIPEDGKILAMDINRENYELGLPVIQKAGLEHKIEFKEGPALPVLDQMIEDGKYHGTYDFIFVDADKDNYINYHKRLIELVKVGGLIGYDNTLWNGSVVAPADAPMRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGITLCRRIK 247          
BLAST of CK933305 vs. ExPASy Swiss-Prot
Match: CAMT_VITVI (Caffeoyl-CoA O-methyltransferase OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 449.899 bits (1156), Expect = 8.463e-126
Identity = 217/238 (91.18%), Postives = 232/238 (97.48%), Query Frame = 2
Query:   92 NQQGG-HKEVGHKSLLQSDALYQYILETSVYPREAESMKELRELTAKHPWNVMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVAHKIDFREGPALPVLDLLIQDEKNHGSFDFIFVDADKDNYLNYHKRLVELVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGVTICRRI 802
            NQ+ G H+EVGHKSLLQSDALYQYILETSVYPRE ESMKELRELTA+HPWN+MTTSADEGQFLNMLLKL+NAKNTMEIGVYTGYSLLATALALPDDGKILAMDIN+ENYELGLPVIQKAGVAHKIDF+EGPALPVLD +I+D K HGSFDFIFVDADKDNYLNYHKRL++LVKVGG+IGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDG+T+CRR+
Sbjct:    4 NQEAGRHQEVGHKSLLQSDALYQYILETSVYPREPESMKELRELTAQHPWNIMTTSADEGQFLNMLLKLINAKNTMEIGVYTGYSLLATALALPDDGKILAMDINKENYELGLPVIQKAGVAHKIDFKEGPALPVLDQMIEDGKYHGSFDFIFVDADKDNYLNYHKRLIDLVKVGGIIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGITLCRRL 241          
BLAST of CK933305 vs. ExPASy Swiss-Prot
Match: CAMT_EUCGU (Caffeoyl-CoA O-methyltransferase OS=Eucalyptus gunnii PE=2 SV=1)

HSP 1 Score: 449.129 bits (1154), Expect = 1.444e-125
Identity = 220/247 (89.07%), Postives = 234/247 (94.74%), Query Frame = 2
Query:   65 MASNAEDQQNQQGGHKEVGHKSLLQSDALYQYILETSVYPREAESMKELRELTAKHPWNVMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVAHKIDFREGPALPVLDLLIQDEKNHGSFDFIFVDADKDNYLNYHKRLVELVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGVTICRRIK 805
            MA+  E+ Q Q G H+EVGHKSLLQSDALYQYILETSVYPRE E MKELRE+TAKHPWN+MTTSADEGQFLNMLLKL+NAKNTMEIGV+TGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVA KIDFREGPALP+LD LI+D K  GSFDFIFVDADKDNYLNYHKRL+ELVKVGG+IGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDG+T+CRRI+
Sbjct:    1 MATAGEESQTQAGRHQEVGHKSLLQSDALYQYILETSVYPREPEPMKELREITAKHPWNIMTTSADEGQFLNMLLKLINAKNTMEIGVFTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVADKIDFREGPALPILDQLIEDGK-QGSFDFIFVDADKDNYLNYHKRLIELVKVGGLIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGITLCRRIQ 246          
BLAST of CK933305 vs. ExPASy Swiss-Prot
Match: CAMT1_EUCGL (Caffeoyl-CoA O-methyltransferase 1 OS=Eucalyptus globulus GN=CCOMT PE=2 SV=1)

HSP 1 Score: 446.817 bits (1148), Expect = 7.164e-125
Identity = 219/246 (89.02%), Postives = 233/246 (94.72%), Query Frame = 2
Query:   65 MASNAEDQQNQQGGHKEVGHKSLLQSDALYQYILETSVYPREAESMKELRELTAKHPWNVMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVAHKIDFREGPALPVLDLLIQDEKNHGSFDFIFVDADKDNYLNYHKRLVELVKVGGVIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGVTICRRI 802
            MA+  E+ Q Q G H+EVGHKSLLQSDALYQ+ILETSVYPRE E MKELRE+TAKHPWN+MTTSADEGQFLNMLLKL+NAKNTMEIGV+TGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVA KIDFREGPALP+LD LI+D K  GSFDFIFVDADKDNYLNYHKRL+ELVKVGG+IGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDG+T+CRRI
Sbjct:    1 MATAGEESQTQAGRHQEVGHKSLLQSDALYQHILETSVYPREPEPMKELREITAKHPWNIMTTSADEGQFLNMLLKLINAKNTMEIGVFTGYSLLATALALPDDGKILAMDINRENYELGLPVIQKAGVADKIDFREGPALPILDQLIEDGK-QGSFDFIFVDADKDNYLNYHKRLIELVKVGGLIGYDNTLWNGSVVAPPDAPLRKYVRYYRDFVLELNKALAADPRIEICMLPVGDGITLCRRI 245          
The following BLAST results are available for this feature:
BLAST of CK933305 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33
Match NameE-valueIdentityDescription
CAMT_POPTM3.671e-12989.47Caffeoyl-CoA O-methyltransferase OS=Populus tremul... [more]
CAMT1_POPTR4.794e-12989.47Caffeoyl-CoA O-methyltransferase 1 OS=Populus tric... [more]
CAMT2_EUCGL1.181e-12787.80Caffeoyl-CoA O-methyltransferase 2 OS=Eucalyptus g... [more]
CAMT_MEDSA2.631e-12787.85Caffeoyl-CoA O-methyltransferase OS=Medicago sativ... [more]
CAMT6_TOBAC7.654e-12788.62Caffeoyl-CoA O-methyltransferase 6 OS=Nicotiana ta... [more]
CAMT4_ARATH2.227e-12687.80Probable caffeoyl-CoA O-methyltransferase At4g3405... [more]
CAMT2_POPTR2.909e-12687.04Caffeoyl-CoA O-methyltransferase 2 OS=Populus tric... [more]
CAMT_VITVI8.463e-12691.18Caffeoyl-CoA O-methyltransferase OS=Vitis vinifera... [more]
CAMT_EUCGU1.444e-12589.07Caffeoyl-CoA O-methyltransferase OS=Eucalyptus gun... [more]
CAMT1_EUCGL7.164e-12589.02Caffeoyl-CoA O-methyltransferase 1 OS=Eucalyptus g... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004287_B11 Developing fruit juice sac at 38 DAFB Citrus sinensis cDNA clone JSJune0002_IIF_B11 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK933305 ID=CK933305; Name=CK933305; organism=Citrus sinensis; type=EST; length=859bp
GCGGGGAACTNNNGTTACTCTTTAAAACTCCANNNTCCAACATCAAAAGA
AAAGACTTGCGTCAATGGCTTCCAACGCAGAAGATCAACAAAACCAACAA
GGCGGACACAAGGAAGTTGGCCACAAGAGCCTGCTCCAATCCGATGCCCT
CTACCAGTATATTCTTGAAACCAGTGTGTACCCAAGAGAGGCTGAATCAA
TGAAGGAGCTTAGAGAATTGACTGCCAAACATCCATGGAACGTTATGACA
ACGTCGGCAGACGAAGGGCAGTTCTTGAACATGCTGTTGAAGCTGGTCAA
TGCAAAGAACACAATGGAGATTGGAGTCTACACAGGCTACTCTCTTTTGG
CCACTGCCCTTGCCCTTCCTGATGACGGAAAGATTTTGGCCATGGACATC
AACAGAGAAAATTATGAATTGGGGCTGCCAGTTATCCAAAAAGCTGGTGT
TGCTCACAAGATTGACTTCAGAGAAGGCCCTGCCCTGCCTGTTCTTGACC
TACTGATCCAAGATGAGAAGAACCATGGGTCCTTTGACTTCATATTTGTG
GACGCTGATAAGGACAACTACCTAAACTACCACAAGAGGTTGGTTGAGTT
GGTGAAGGTTGGAGGGGTGATCGGGTACGACAACACCCTATGGAACGGAT
CCGTCGTGGCGCCACCTGATGCTCCCCTTAGGAAGTACGTTAGGTATTAC
CGAGACTTCGTCTTGGAGCTCAACAAGGCCCTTGCTGCTGACCCCAGGAT
TGAAATCTGCATGCTCCCCGTTGGCGATGGAGTCACTATCTGCCGTCGGA
TCAAGTGAAATGCCTAAACCAATTCTCTGNNNGGGTCCGCCGCAGATGAC
CGGTACCTC
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