CK934143

Overview
NameCK934143
Unique NameCK934143
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length838
Libraries
Library NameType
Developing fruit peel at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK934143 vs. ExPASy Swiss-Prot
Match: CHI1_TOBAC (Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1 SV=2)

HSP 1 Score: 296.975 bits (759), Expect = 8.882e-80
Identity = 137/224 (61.16%), Postives = 168/224 (75.00%), Query Frame = 2
Query:  179 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCR--GKYYGRGPIQLSRNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGQGGNAAVRNRIGFFTTF 838
            +LG IIS  MFD +L++RND  C  +GFY+Y+AFI AA++FPGFG SG+ T RKREIAAFFAQT HETTGGW  AP G YAWGYC++ E   P DYC P+  +PC    KY+GRGPIQ+S NYNY  C           NNPDL+ATDPV+SFKSA+WFWMT Q PKPSCH+VII  W+PSA D  A RLPG+G+ TNIINGG+ECG+G ++ V++RIGF+  +
Sbjct:   79 DLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPC--GRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGRWQPSAGDRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRY 300          
BLAST of CK934143 vs. ExPASy Swiss-Prot
Match: CHI2_TOBAC (Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1 SV=1)

HSP 1 Score: 295.049 bits (754), Expect = 3.375e-79
Identity = 136/224 (60.71%), Postives = 168/224 (75.00%), Query Frame = 2
Query:  179 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCR--GKYYGRGPIQLSRNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGQGGNAAVRNRIGFFTTF 838
            +LG IIS  MFD +L++RND  C  +GFY+Y+AFI AA++FPGFG SG+ T RKREIAAFFAQT HETTGGW  AP G YAWGYC++ E   P DYC P+  +PC    KY+GRGPIQ+S NYNY  C           NNPDL+ATDPV+SFKSA+WFWMT Q PKPSCH+VII  W+PS+ D  A RLPG+G+ TNIINGG+ECG+G ++ V++RIGF+  +
Sbjct:   74 DLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPC--GRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGRWQPSSADRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRY 295          
BLAST of CK934143 vs. ExPASy Swiss-Prot
Match: CHI2_BRANA (Endochitinase CH25 OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 5.757e-79
Identity = 135/224 (60.27%), Postives = 167/224 (74.55%), Query Frame = 2
Query:  179 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSRNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGQGGNAAVRNRIGFFTTF 838
            +L  IISR  FDD+L++RND  CPARGFYTYDAFI AA++FPGFG +G+   RK+EIAAFF QT HETTGGW  AP G Y+WGYCF  E +P S+YC P+  +PC     YYGRGP+QLS NYNY +C           NNPDL++ DPV++FK+AIWFWMT Q PKPSCH VI+ +W+PS  D  AGR+PGYG+ TNIINGG+ECG+G +A V +RIGF+  +
Sbjct:   70 DLSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFPGFGTTGDTATRKKEIAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPSSNYCSPSAEWPCASGKSYYGRGPMQLSWNYNYGQC--GRAIGSDLLNNPDLVSNDPVIAFKAAIWFWMTPQSPKPSCHAVIVGQWQPSDADRAAGRVPGYGVITNIINGGLECGRGQDARVADRIGFYQRY 291          
BLAST of CK934143 vs. ExPASy Swiss-Prot
Match: CHIT_CARPA (Endochitinase OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 1.675e-78
Identity = 135/221 (61.09%), Postives = 165/221 (74.66%), Query Frame = 2
Query:  188 KIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYC--DPNYPCR-GK-YYGRGPIQLSRNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGQGGNAAVRNRIGFFTTF 838
            KIISR MFD +L++RN+  CPA+GFYTYDAF+ AA++FP FG +G+  +RKRE+AAF  QT HETTGGWP AP G YAWGYCF+ E +P S+YC   P YPC  GK YYGRGP+QLS NYNY  C            NPDL+ATD VLSFK+A+WFWMT Q PKPSCH+V+   W+PSA D  AGRLPGYG+ TN++NGG+ECG+G N  V +R+GFF  +
Sbjct:    4 KIISRSMFDQMLKHRNNPACPAKGFYTYDAFLAAAKSFPSFGTTGSTDVRKRELAAFLGQTSHETTGGWPSAPDGPYAWGYCFLKERNPSSNYCAPSPRYPCAPGKSYYGRGPLQLSWNYNYGPC--GEALRVNLLGNPDLVATDRVLSFKTALWFWMTPQAPKPSCHDVLTGRWQPSAADTAAGRLPGYGVLTNLLNGGLECGKGPNPQVADRLGFFRRY 222          
BLAST of CK934143 vs. ExPASy Swiss-Prot
Match: CHIB_ARATH (Basic endochitinase B OS=Arabidopsis thaliana GN=CHI-B PE=1 SV=3)

HSP 1 Score: 292.352 bits (747), Expect = 2.188e-78
Identity = 135/224 (60.27%), Postives = 166/224 (74.11%), Query Frame = 2
Query:  179 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSRNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGQGGNAAVRNRIGFFTTF 838
            +L  IIS   FDD+L++RND  CPARGFYTY+AFI AA++FPGFG +G+   RK+E+AAFF QT HETTGGW  AP G Y+WGYCF  E +P SDYC+P+  +PC    +YYGRGP+QLS NYNY  C           NNPDL+A D V++FK+AIWFWMTAQPPKPSCH VI  +W+PS  D  AGRLPGYG+ TNIINGG+ECG+G +  V +RIGF+  +
Sbjct:   85 DLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCASGKRYYGRGPMQLSWNYNYGLC--GRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHAVIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRY 306          
BLAST of CK934143 vs. ExPASy Swiss-Prot
Match: CHI4_SOLTU (Endochitinase 4 (Fragment) OS=Solanum tuberosum GN=CHTB4 PE=2 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 8.314e-78
Identity = 135/225 (60.00%), Postives = 167/225 (74.22%), Query Frame = 2
Query:  179 ELGKIISREMFDDLLEYRNDERCPARG-FYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSRNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGQGGNAAVRNRIGFFTTF 838
            ++G +IS  MFD +L++RND  C  +  FY+Y+AFI AA++F GFG +G+ T RKREIAAFFAQT HETTGGWP AP G YAWGYCF+ E   P DYC P+  +PC    KY+GRGPIQ+S NYNY  C           NNPDL+ATDPV+SFKSAIWFWMT Q PKPSCH+VII  W+PSA D  A RLPG+G+ TNIINGG+ECG+G ++ V++RIGF+  +
Sbjct:   77 DIGSVISNSMFDQMLKHRNDNACQGKNNFYSYNAFINAARSFGGFGTTGDTTARKREIAAFFAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLL--NNPDLVATDPVISFKSAIWFWMTPQSPKPSCHDVIIGRWQPSAADRAANRLPGFGVITNIINGGLECGRGSDSRVQDRIGFYRRY 299          
BLAST of CK934143 vs. ExPASy Swiss-Prot
Match: CHI1_GOSHI (Endochitinase 1 OS=Gossypium hirsutum PE=3 SV=1)

HSP 1 Score: 287.73 bits (735), Expect = 5.389e-77
Identity = 140/225 (62.22%), Postives = 167/225 (74.22%), Query Frame = 2
Query:  182 LGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGG--WPDAPGGEYAWGYCFISEVSPPSDYC--DPNYPCR-GK-YYGRGPIQLSRNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGQGGNAAVRNRIGFFTTF 838
            L  +ISRE F+ +L +RND  CPARGFYTYDAFI AA++FP F  +G++  RKREIAAF AQT HETTGG  W  AP G YAWGYC+  E++PPS YC  DPNYPC  GK Y+GRGP+QLS NYNY +C           NNPDLL++DP +SFKSA WFWMT Q PKPSCH VII  W PS++D  AGR+PGYG+ TNIINGG+ECG+G NA V +RIGF+  +
Sbjct:   74 LTNLISRETFNQMLLHRNDGACPARGFYTYDAFIAAARSFPAFATTGDQATRKREIAAFLAQTSHETTGGAGWA-APDGPYAWGYCYNRELNPPSSYCASDPNYPCAPGKQYFGRGPMQLSWNYNYGQCGRAIGVDLL--NNPDLLSSDPTISFKSAFWFWMTPQSPKPSCHNVIIGAWSPSSSDRAAGRVPGYGVITNIINGGLECGKGWNAQVEDRIGFYKRY 295          
BLAST of CK934143 vs. ExPASy Swiss-Prot
Match: CHIT_SOLTU (Endochitinase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 2.048e-76
Identity = 132/224 (58.93%), Postives = 163/224 (72.77%), Query Frame = 2
Query:  179 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCR--GKYYGRGPIQLSRNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGQGGNAAVRNRIGFFTTF 838
            +LG  IS  MFD +L++RN+  C  + FY+Y+AFI AA++FPGFG SG+   RKREIAAFFAQT HETTGGW  AP G YAWGYCF+ E   P DYC P+  +PC    KY+GRGPIQ+S NYNY  C           NNPDL+ATDPV+SFK+A+WFWMT Q PKPSCH+VII  W PS+ D  A RLPG+G+ TNIINGG+ECG+G +  V++RIGF+  +
Sbjct:   78 DLGSAISNSMFDQMLKHRNENSCQGKNFYSYNAFINAARSFPGFGTSGDINARKREIAAFFAQTSHETTGGWASAPDGPYAWGYCFLRERGNPGDYCPPSSQWPCAPGRKYFGRGPIQISHNYNYGPC--GRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHDVIIGRWNPSSADRAANRLPGFGVITNIINGGLECGRGTDNRVQDRIGFYRRY 299          
BLAST of CK934143 vs. ExPASy Swiss-Prot
Match: CHIB_VITVI (Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE=2 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 2.048e-76
Identity = 137/247 (55.47%), Postives = 170/247 (68.83%), Query Frame = 2
Query:  110 SAQNCGSGVVYXXXXXXXXXXXXELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSRNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGQGGNAAVRNRIGFFTTF 838
            +A  CGSG               ++G++I+R MF+D+L++RN+  CP +GFYTYDAFI AA+AFPGFG +G+ T RKREIAAF AQT HETTGGW  AP G YAWGYC++ E   P  YC P+  +PC    KYYGRGPIQ+S NYNY +            NNPDL+ATD V+SFK+A WFWMT Q PKPSCH VI   W PS  D +AGRLPG+G+ TNIINGG+ECG+G    V++RIGF+  +
Sbjct:   47 TADYCGSGC------QSQCSSTGDIGQLITRSMFNDMLKHRNEGSCPGKGFYTYDAFIAAAKAFPGFGTTGDTTTRKREIAAFLAQTSHETTGGWASAPDGPYAWGYCYLREQGSPGAYCVPSAQWPCAAGRKYYGRGPIQISYNYNYGQA--GKAIGVDLVNNPDLVATDAVISFKTAFWFWMTPQSPKPSCHNVITGGWTPSGADRSAGRLPGFGVITNIINGGVECGKGVVPQVQDRIGFYKRY 285          
BLAST of CK934143 vs. ExPASy Swiss-Prot
Match: CHI1_SOLTU (Endochitinase 1 (Fragment) OS=Solanum tuberosum GN=CHTB1 PE=2 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 2.048e-76
Identity = 134/225 (59.56%), Postives = 163/225 (72.44%), Query Frame = 2
Query:  179 ELGKIISREMFDDLLEYRNDERCPARG-FYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSRNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGQGGNAAVRNRIGFFTTF 838
            +LG +IS  MFD +L +RND  C  +G FY+Y+AFI AA +FPGFG +G+ T RKREIAAFFAQT HETTGGWP AP G YAWGYCF+ E   P DYC P+  +PC    KY+GRGPIQ+S NYNY  C           NNPDL+ATD V+SFKSAIWFWMT Q PKPSCH+VI   W+PS  D  A R+PG+G+ TNIINGG+ECG G ++ V++RIGF+  +
Sbjct:   67 DLGGVISNSMFDQMLNHRNDNACQGKGNFYSYNAFISAAGSFPGFGTTGDITARKREIAAFFAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLL--NNPDLVATDSVISFKSAIWFWMTPQSPKPSCHDVITGRWQPSGVDQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIGFYRRY 289          
The following BLAST results are available for this feature:
BLAST of CK934143 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 56
Match NameE-valueIdentityDescription
CHI1_TOBAC8.882e-8061.16Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1... [more]
CHI2_TOBAC3.375e-7960.71Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1... [more]
CHI2_BRANA5.757e-7960.27Endochitinase CH25 OS=Brassica napus PE=2 SV=1[more]
CHIT_CARPA1.675e-7861.09Endochitinase OS=Carica papaya PE=1 SV=1[more]
CHIB_ARATH2.188e-7860.27Basic endochitinase B OS=Arabidopsis thaliana GN=C... [more]
CHI4_SOLTU8.314e-7860.00Endochitinase 4 (Fragment) OS=Solanum tuberosum GN... [more]
CHI1_GOSHI5.389e-7762.22Endochitinase 1 OS=Gossypium hirsutum PE=3 SV=1[more]
CHIT_SOLTU2.048e-7658.93Endochitinase OS=Solanum tuberosum PE=2 SV=1[more]
CHIB_VITVI2.048e-7655.47Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE... [more]
CHI1_SOLTU2.048e-7659.56Endochitinase 1 (Fragment) OS=Solanum tuberosum GN... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004540_H05 Developing fruit peel at 38 DAFB Citrus sinensis cDNA clone P38DAB10001_IF_H05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK934143 ID=CK934143; Name=CK934143; organism=Citrus sinensis; type=EST; length=838bp
GGGGATCACCAAGCAGAGGATAATTGATATCATAACTGTNNNTTTCAAAA
TGAGGCTTATTGGGTCCTTACTAATTTTCTCTCTAGTTTTATCTTTTGTA
CTAGGAGGCTCAGCACAGAACTGTGGAAGCGGCGTTGTGTACGGTGGACG
TGATACCGGCCATGGGACGCATGGCGGTGAACTGGGCAAGATCATCTCAA
GGGAGATGTTTGATGACTTGCTTGAGTATAGAAATGATGAACGATGCCCT
GCCAGAGGCTTCTACACTTATGATGCTTTTATAGAGGCAGCCCAAGCTTT
TCCAGGCTTTGGTAACTCTGGAAATGAAACCATGCGTAAAAGAGAAATCG
CTGCCTTTTTTGCCCAAACTGGCCATGAAACTACTGGGGGATGGCCCGAT
GCACCTGGTGGCGAATATGCTTGGGGATATTGCTTTATAAGCGAAGTTAG
CCCTCCGTCCGACTACTGTGATCCTAACTATCCATGTCGAGGGAAGTACT
ATGGCCGAGGTCCAATTCAACTCTCTCGGAATTACAATTACTTACGGTGC
GGAGAAGGCCTAGGGCTAGGAGAGGAGTTGTTGAACAATCCAGACCTTCT
TGCTACTGATCCAGTACTATCATTCAAGTCAGCAATCTGGTTCTGGATGA
CTGCACAGCCACCAAAGCCATCGTGCCACGAAGTCATTATCGACGAATGG
AAACCATCAGCAAATGACGTAAACGCCGGCCGGCTTCCGGGATACGGTCT
AACCACAAATATAATCAACGGTGGAATTGAATGTGGCCAAGGTGGGAATG
CCGCTGTACGTAACCGTATTGGGTTCTTTACCACTTTC
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