CK934604

Overview
NameCK934604
Unique NameCK934604
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length876
Libraries
Library NameType
Developing fruit peel at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK934604 vs. ExPASy Swiss-Prot
Match: GUN25_ARATH (Endoglucanase 25 OS=Arabidopsis thaliana GN=KOR PE=1 SV=1)

HSP 1 Score: 362.844 bits (930), Expect = 1.401e-99
Identity = 174/221 (78.73%), Postives = 189/221 (85.52%), Query Frame = 2
Query:  212 MFGRDPWGGPLEINXXXXXXXXXXXXNLQDFDRAALSRPLDETQQSWLLGPTEKKKKKYVDLGCIIVSRKIFVWTVGTLLVSALLAGFITLIVKTVPRHHHSHARPDNYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCLKDGKSASSSISKDLVGGFYDAGDAIKFNFPQSFTITMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLKTLHHSADS 874
            M+GRDPWGGPLEIN            NL D DRAALSRPLDETQQSWLLGPTE+KKKKYVDLGCIIVSRKIFVWTVGTL+ +ALLAGFITLIVKTVPRHH     PDNYT+ALHKAL FFNAQ+SGKLPKHNNVSWRGNS L+DGK  + S  KDLVGG+YDAGDAIKFNFP ++ +TMLSWSVIEYSAKYEAAGEL HVKE+IKWGTDYFLKT + +ADS
Sbjct:    1 MYGRDPWGGPLEINTADSATDDDRSRNLNDLDRAALSRPLDETQQSWLLGPTEQKKKKYVDLGCIIVSRKIFVWTVGTLVAAALLAGFITLIVKTVPRHHPKTPPPDNYTIALHKALKFFNAQKSGKLPKHNNVSWRGNSGLQDGKGETGSFYKDLVGGYYDAGDAIKFNFPMAYAMTMLSWSVIEYSAKYEAAGELTHVKELIKWGTDYFLKTFNSTADS 221          
BLAST of CK934604 vs. ExPASy Swiss-Prot
Match: GUN21_ARATH (Endoglucanase 21 OS=Arabidopsis thaliana GN=KOR3 PE=2 SV=1)

HSP 1 Score: 331.643 bits (849), Expect = 3.458e-90
Identity = 159/222 (71.62%), Postives = 181/222 (81.53%), Query Frame = 2
Query:  212 MFGRDPWGGPLEINXXXXXXXXXXXXNLQDFDRAALSRPLDETQQSWLLGPTEKKKKKYVDLGCIIVSRKIFVWTVGTLLVSALLAGFITLIVKTVPRHHHSHARPDNYTLALHKALMFFNAQRSGKLPKH-NNVSWRGNSCLKDGKSASSSISKDLVGGFYDAGDAIKFNFPQSFTITMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLKTLHHSADS 874
            M+GRDPWGGPLEIN            NLQD DRA  SRPLDETQQSWLLGP   KKKKYVDLGCI+VSRKIF+WT+GT++V+ALL+GFITLIVKT+P HHH    PDNYT+AL  AL FFNAQ+SGKLPK+  NVSWR +SCL+DGK       KDLVGG+YDAGD+IKFNFP S+ +TMLSWSVIEYSAKY+AAGEL HVKE+IKWGTDYFLKT + SAD+
Sbjct:    1 MYGRDPWGGPLEINAADSMTDDDRSRNLQDLDRATPSRPLDETQQSWLLGPKVLKKKKYVDLGCILVSRKIFLWTLGTIVVTALLSGFITLIVKTLPHHHHKEPPPDNYTIALRTALKFFNAQQSGKLPKNIYNVSWRHDSCLQDGKGDPGQCYKDLVGGYYDAGDSIKFNFPMSYAMTMLSWSVIEYSAKYQAAGELEHVKELIKWGTDYFLKTFNSSADN 222          
BLAST of CK934604 vs. ExPASy Swiss-Prot
Match: GUN9_ORYSJ (Endoglucanase 9 OS=Oryza sativa subsp. japonica GN=GLU1 PE=2 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 2.484e-80
Identity = 145/226 (64.16%), Postives = 175/226 (77.43%), Query Frame = 2
Query:  206 MSMFGRDPWGGPLEINXXXXXXXXXXXXNLQDFDRAALSRPLDETQQSWLL---GPTEKKKKKYVDLGCIIVSRKIFVWTVGTLLVSALLAGFITLIVKTVPRHHHSHARPDNYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCLKDGKSASSSISKDLVGGFYDAGDAIKFNFPQSFTITMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLKTLHHSADS 874
            MSM+GRDPWGGPLEI             NL D DR ALSR LDETQQSWLL   G   +KKKKYVDLGC++VSRK+FVWTVG LL +A+ AG +  I K +PRHH     PD++T+AL KALMFFNAQ+SGKLPK+NNV WRGNSC+KDG S   ++ + LVGG+YDAGDA+KFNFP +F++T+LSWSVIEYSAKYEA GEL H+++ IKWG DYFLKT + +AD+
Sbjct:    1 MSMYGRDPWGGPLEI-CHDSATDDDRSRNL-DLDRGALSRTLDETQQSWLLAGPGDQGRKKKKYVDLGCLVVSRKLFVWTVGVLLAAAVFAGLVAGIAKAIPRHHRPPPPPDDFTVALRKALMFFNAQKSGKLPKNNNVHWRGNSCMKDGLS-DPAVGRSLVGGYYDAGDAVKFNFPAAFSMTLLSWSVIEYSAKYEAVGELGHIRDTIKWGADYFLKTFNSTADT 223          
BLAST of CK934604 vs. ExPASy Swiss-Prot
Match: GUN10_ORYSJ (Endoglucanase 10 OS=Oryza sativa subsp. japonica GN=GLU2 PE=2 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 1.610e-79
Identity = 149/225 (66.22%), Postives = 171/225 (76.00%), Query Frame = 2
Query:  212 MFGRDPWGGPLEINXXXXXXXXXXXXNLQDFDRAALSRPLDETQQSWLL-GPTE---KKKKKYVDLGCIIVSRKIFVWTVGTLLVSALLAGFITLIVKTVPRHHHSHARPDNYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCLKDGKSASSSISKDLVGGFYDAGDAIKFNFPQSFTITMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLKTLHHSADS 874
            MFGRDPWGGPLEI+              +D DR AL R LDETQQSWLL GP +   KKKKKYVDLGC+++ RKIF+WTVGT+L   L  GF+ +IVK VP        PD YT ALHKALMFFNAQRSG LPKHN VSWRGNSC+KDG S  S++ K LVGGFYDAGDAIKFN+P ++++TMLSWSVIEY AKYEA GEL+HVKE+IKWGTDY LKT + SAD+
Sbjct:    1 MFGRDPWGGPLEISNADSATDDDRS---RDLDRGALMRQLDETQQSWLLAGPGDQAGKKKKKYVDLGCMVLDRKIFMWTVGTILGVGLFIGFVMMIVKLVPHKRPPPPPPDQYTQALHKALMFFNAQRSGPLPKHNGVSWRGNSCMKDGLS-DSTVRKSLVGGFYDAGDAIKFNYPMAWSMTMLSWSVIEYKAKYEAIGELDHVKELIKWGTDYLLKTFNSSADT 221          
BLAST of CK934604 vs. ExPASy Swiss-Prot
Match: GUN12_ORYSJ (Endoglucanase 12 OS=Oryza sativa subsp. japonica GN=GLU3 PE=2 SV=2)

HSP 1 Score: 239.195 bits (609), Expect = 2.336e-62
Identity = 123/225 (54.67%), Postives = 153/225 (68.00%), Query Frame = 2
Query:  212 MFGRDPWGGPLEINXXXXXXXXXXXXNLQDFDRAALS---RPLDETQQSWLLGPTEKKKK-KYVDLGCIIVSRKIFVWTVGTLLVSALLAGFITLIVKTVPRHHHSHARPDNYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCLKDGKSASSSISKDLVGGFYDAGDAIKFNFPQSFTITMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLKTLHHSADS 874
            M+  + WGG  EI                D DR ALS     LDETQQSWLLGP E KKK KYVDLGC++V RK+  W + TLL + +L G   +I K++P+       PD YT ALHKAL+FFNAQ+SG+LPK+N + WRGNS L DG S  + +   LVGG+YDAGD IKF+FP +F++TMLSWSVIEYSAKY+A GE +HV+E+IKWGTDY L T + SA +
Sbjct:    1 MYSANHWGGSFEIAADGAAEDDHSRN--MDLDRGALSARQHQLDETQQSWLLGPPEAKKKDKYVDLGCVVVKRKLLWWVLWTLLAAFILIGLPVIIAKSIPKKKPHAPPPDQYTDALHKALLFFNAQKSGRLPKNNGIKWRGNSGLSDG-SDLTDVKGGLVGGYYDAGDNIKFHFPLAFSMTMLSWSVIEYSAKYKAVGEYDHVRELIKWGTDYLLLTFNSSAST 222          
BLAST of CK934604 vs. ExPASy Swiss-Prot
Match: GUN7_ARATH (Endoglucanase 7 OS=Arabidopsis thaliana GN=KOR2 PE=2 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 1.015e-57
Identity = 113/221 (51.13%), Postives = 150/221 (67.87%), Query Frame = 2
Query:  230 WGGPLEI--NXXXXXXXXXXXXNLQDFDRAALSRP---LDETQQSWLLGPTE---KKKKKYVDLGCIIVSRKIFVWTVGTLLVSALLAGFITLIVKTVPRHHHSHARPDNYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCLKDGKSASSSISKDLVGGFYDAGDAIKFNFPQSFTITMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLKTLHHSA 868
            WGG L+   +            N  ++DR A+      LDETQQ WLL P +   KKKKKYV+LGC+ VSR +F+WTVG++ V  L+     +IVK++PRH  +   PDNYTLALHKAL FF+AQ+SGKLPK N VSWRG+S  KDG      +   LVGG+YD G  +KF+FP +F++TMLSWS+IEYS KY+A  E +H+++++KWGTDY L T ++SA
Sbjct:    7 WGGSLDAVDSDRIAAEEEERLRNTTEWDRGAIHSQRSELDETQQGWLLAPQDNWRKKKKKYVNLGCVSVSRTVFLWTVGSIAVLFLVVALPIIIVKSLPRHKSAPPPPDNYTLALHKALQFFDAQKSGKLPKKNKVSWRGDSGTKDG---LPDVVGGLVGGYYDGGSNVKFHFPMAFSMTMLSWSLIEYSHKYKAIDEYDHMRDVLKWGTDYLLLTFNNSA 224          
BLAST of CK934604 vs. ExPASy Swiss-Prot
Match: GUN7_ORYSJ (Endoglucanase 7 OS=Oryza sativa subsp. japonica GN=GLU10 PE=2 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 3.079e-30
Identity = 68/135 (50.37%), Postives = 86/135 (63.70%), Query Frame = 2
Query:  446 LLVSALLAGFITLIVKTVPRHHHSHARPDNYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCLKDGKSASSSISKDLVGGFYDAGDAIKFNFPQSFTITMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLK 850
            +LV+ALL   + L          +  +  NY  AL K+L++F AQRSG+LP +  V+WR +S L DG         DLVGG+YDAGD +KF  P +FT+TMLSWS+IEY    EAAGEL H  E IKWGTDYF+K
Sbjct:    7 VLVAALLLQLLLLAAAGGAGAAATERKAHNYEDALRKSLLYFEAQRSGRLPHNQRVAWRDHSGLTDGLEQGV----DLVGGYYDAGDHVKFGLPMAFTVTMLSWSMIEYGDDVEAAGELGHALEAIKWGTDYFIK 137          
BLAST of CK934604 vs. ExPASy Swiss-Prot
Match: GUN16_ORYSJ (Endoglucanase 16 OS=Oryza sativa subsp. japonica GN=Os06g0247900 PE=2 SV=2)

HSP 1 Score: 129.028 bits (323), Expect = 3.404e-29
Identity = 67/138 (48.55%), Postives = 84/138 (60.87%), Query Frame = 2
Query:  437 VGTLLVSALLAGFITLIVKTVPRHHHSHARPDNYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCLKDGKSASSSISKDLVGGFYDAGDAIKFNFPQSFTITMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLK 850
            VG ++  ALL G          RH        +Y  AL K+L++F AQRSG+LP    V+WR +S L DG         DLVGG+YDAGD +KF  P +FT+TMLSWS++EY A   AAGEL H  + IKWGTDYF+K
Sbjct:    6 VGDVVAVALLLGAAAAAAAAAARH--------DYEEALRKSLLYFEAQRSGRLPHGQRVAWRDHSGLTDGLEQGV----DLVGGYYDAGDHVKFGLPMAFTVTMLSWSLLEYGADVAAAGELAHALDAIKWGTDYFIK 131          
BLAST of CK934604 vs. ExPASy Swiss-Prot
Match: GUN5_ORYSJ (Endoglucanase 5 OS=Oryza sativa subsp. japonica GN=Os02g0151300 PE=2 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 9.904e-29
Identity = 62/114 (54.39%), Postives = 76/114 (66.67%), Query Frame = 2
Query:  521 ARPDNYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCLKDGKSASSSISKDLVGGFYDAGDAIKFNFPQSFTITMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLKTLHH 862
            A   +Y  AL K+L++F AQRSG+LP +  V WRG+S L DG         DLVGG+YDAGD +KF  P +FT+TMLSWSV+EY  +  AAGEL H    IKWGTDYF+K   H
Sbjct:   25 AAAHDYGEALSKSLLYFEAQRSGRLPYNQRVRWRGHSGLTDGLEQGV----DLVGGYYDAGDHVKFGLPMAFTVTMLSWSVLEYGEEIAAAGELGHALHAIKWGTDYFIKAHTH 134          
BLAST of CK934604 vs. ExPASy Swiss-Prot
Match: GUN2_ORYSJ (Endoglucanase 2 OS=Oryza sativa subsp. japonica GN=GLU5 PE=1 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 1.293e-28
Identity = 59/106 (55.66%), Postives = 75/106 (70.75%), Query Frame = 2
Query:  533 NYTLALHKALMFFNAQRSGKLPKHNNVSWRGNSCLKDGKSASSSISKDLVGGFYDAGDAIKFNFPQSFTITMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLK 850
            +Y +AL K++++F AQRSG LP    ++WR NS L DGK+       DLVGG+YDAGD +KF  P +FT+TM++WSVIEY  +  AAGEL H  E IKWGTDYF K
Sbjct:   40 DYGMALSKSILYFEAQRSGVLPGSQRIAWRANSGLADGKANGV----DLVGGYYDAGDNVKFGLPMAFTVTMMAWSVIEYGEEMAAAGELGHAVEAIKWGTDYFAK 141          
The following BLAST results are available for this feature:
BLAST of CK934604 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 62
Match NameE-valueIdentityDescription
GUN25_ARATH1.401e-9978.73Endoglucanase 25 OS=Arabidopsis thaliana GN=KOR PE... [more]
GUN21_ARATH3.458e-9071.62Endoglucanase 21 OS=Arabidopsis thaliana GN=KOR3 P... [more]
GUN9_ORYSJ2.484e-8064.16Endoglucanase 9 OS=Oryza sativa subsp. japonica GN... [more]
GUN10_ORYSJ1.610e-7966.22Endoglucanase 10 OS=Oryza sativa subsp. japonica G... [more]
GUN12_ORYSJ2.336e-6254.67Endoglucanase 12 OS=Oryza sativa subsp. japonica G... [more]
GUN7_ARATH1.015e-5751.13Endoglucanase 7 OS=Arabidopsis thaliana GN=KOR2 PE... [more]
GUN7_ORYSJ3.079e-3050.37Endoglucanase 7 OS=Oryza sativa subsp. japonica GN... [more]
GUN16_ORYSJ3.404e-2948.55Endoglucanase 16 OS=Oryza sativa subsp. japonica G... [more]
GUN5_ORYSJ9.904e-2954.39Endoglucanase 5 OS=Oryza sativa subsp. japonica GN... [more]
GUN2_ORYSJ1.293e-2855.66Endoglucanase 2 OS=Oryza sativa subsp. japonica GN... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004229_A05 Developing fruit peel at 38 DAFB Citrus sinensis cDNA clone P38DAB10002_IVF_A05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK934604 ID=CK934604; Name=CK934604; organism=Citrus sinensis; type=EST; length=876bp
GGGATGGAAAAATTCAGCAAGTAATTTGACAGNNNGATCAGAGCACCACC
GTCACCACCAAATCACACCACACAACTCTACAAAACCAGACCGGAGCATT
CACAGTACAAACTTTCCATCTTAATTCTTGAGAGAGAAAGAGATCTAACA
TATCTTTAGCTGAGGGGAGAGTGAGTGAGTGTGAGATTTGATTTGGTAAC
AAACAATGAGTATGTTTGGAAGAGATCCGTGGGGTGGCCCACTGGAGATC
AACGCGGCGGACTCAGCCACTGACGACGATCGGAGCAGGAACCTTCAGGA
TTTCGACAGGGCAGCTCTGTCTCGGCCCTTGGATGAGACCCAGCAGAGCT
GGCTTCTGGGTCCCACCGAAAAGAAGAAGAAGAAGTACGTAGATCTGGGT
TGCATCATCGTCAGCCGTAAGATCTTCGTCTGGACTGTCGGCACTCTTCT
CGTCTCCGCACTCTTAGCCGGCTTCATCACTCTTATTGTCAAAACGGTGC
CGCGTCATCACCACTCTCATGCTCGCCCTGATAATTACACTCTCGCTTTG
CACAAGGCCCTCATGTTCTTCAATGCCCAACGATCTGGCAAATTACCCAA
GCATAATAACGTTTCTTGGAGGGGAAATTCTTGTCTGAAGGATGGCAAAT
CGGCTTCCTCTAGTATTTCCAAGGATCTGGTTGGGGGTTTTTACGATGCT
GGGGATGCCATTAAGTTTAATTTTCCTCAATCTTTTACTATTACCATGCT
CAGCTGGAGTGTGATTGAGTACAGTGCTAAGTACGAAGCTGCTGGTGAGC
TTAACCATGTTAAGGAAATTATCAAGTGGGGTACTGATTACTTTCTCAAG
ACCCTTCATCACTCTGCTGATTCCAT
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