EY692791

Overview
NameEY692791
Unique NameEY692791
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length930
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY692791 vs. ExPASy Swiss-Prot
Match: PSB4_ARATH (Proteasome subunit beta type-4 OS=Arabidopsis thaliana GN=PBG1 PE=1 SV=2)

HSP 1 Score: 218.009 bits (554), Expect = 1.516e-59
Identity = 101/128 (78.91%), Postives = 117/128 (91.41%), Query Frame = 3
Query:  144 QRTQLPYVTGTSVVALKYKDGILMAADMGGSYGSTIRYKSVERIKPIGKHSLLGASGEISDFQELSRNLDELILYDNMWDDGNSLGPKELHSYLTRVMYNMRHKLDPLWNSLVLGGVQNGHEYLGVVS 527
            QRT  PYVTGTS+VA+KYKDG+LMA+DMGGSYGST+RYK++ER+K IGKHSLLGASGEISDFQE+ R LDEL L DNMWDDGNSLGPKE+H+YLTRVMYN R+K +PLWN+LVLGGV+NG  YLG+VS
Sbjct:   22 QRTLYPYVTGTSIVAIKYKDGVLMASDMGGSYGSTLRYKNIERVKAIGKHSLLGASGEISDFQEILRYLDELTLNDNMWDDGNSLGPKEIHNYLTRVMYNRRNKFNPLWNTLVLGGVKNGKSYLGMVS 149          

HSP 2 Score: 33.8834 bits (76), Expect = 1.516e-59
Identity = 14/16 (87.50%), Postives = 15/16 (93.75%), Query Frame = 2
Query:  527 MIGVNFEDSHVATGFG 574
            MIGV+FED HVATGFG
Sbjct:  150 MIGVSFEDDHVATGFG 165          
BLAST of EY692791 vs. ExPASy Swiss-Prot
Match: PSB4_BOVIN (Proteasome subunit beta type-4 OS=Bos taurus GN=PSMB4 PE=1 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 2.105e-32
Identity = 62/129 (48.06%), Postives = 95/129 (73.64%), Query Frame = 3
Query:  138 PIQRTQLPYVTGTSVVALKYKDGILMAADMGGSYGSTIRYKSVERIKPIGKHSLLGASGEISDFQELSRNLDELILYDNMWDDGNSLGPKELHSYLTRVMYNMRHKLDPLWNSLVLGGVQNGHEYLGVV 524
            PI RTQ P VTGTSV+ LK++ G+++AADM GSYGS  R++++ RI  +   ++LGASG+ +DFQ L + L ++++ + +  DG+S  PK +HS+LTR MY+ R K++PLWN++V+GG  +G  +LG V
Sbjct:   42 PITRTQNPMVTGTSVLGLKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPKAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYV 170          

HSP 2 Score: 21.9422 bits (45), Expect = 2.105e-32
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = 2
Query:  527 MIGVNFEDSHVATGFGITL 583
            M+GV +E   +ATG+G  L
Sbjct:  172 MLGVAYEAPSLATGYGAYL 190          
BLAST of EY692791 vs. ExPASy Swiss-Prot
Match: PSB4_XENLA (Proteasome subunit beta type-4 (Fragment) OS=Xenopus laevis GN=psmb4 PE=2 SV=2)

HSP 1 Score: 139.043 bits (349), Expect = 2.746e-32
Identity = 59/129 (45.74%), Postives = 97/129 (75.19%), Query Frame = 3
Query:  138 PIQRTQLPYVTGTSVVALKYKDGILMAADMGGSYGSTIRYKSVERIKPIGKHSLLGASGEISDFQELSRNLDELILYDNMWDDGNSLGPKELHSYLTRVMYNMRHKLDPLWNSLVLGGVQNGHEYLGVV 524
            P++ T  P VTGTSV+ +K+  G+++AADM GSYGS  R++++ RI  + ++++LGASG+ +D+Q L + +D++++ + +  DG++  PK +HS+LTRVMYN R K++PLWN++V+GG  NG  +LG V
Sbjct:   20 PVRHTLNPMVTGTSVLGVKFDGGVIIAADMLGSYGSLARFRNISRIMKVNENTILGASGDYADYQYLKQVIDQMVIDEELVGDGHNYSPKAIHSWLTRVMYNRRSKMNPLWNTVVIGGFYNGESFLGYV 148          

HSP 2 Score: 21.557 bits (44), Expect = 2.746e-32
Identity = 9/18 (50.00%), Postives = 12/18 (66.67%), Query Frame = 2
Query:  530 IGVNFEDSHVATGFGITL 583
            +GV +E   +ATGFG  L
Sbjct:  151 LGVAYEAPTIATGFGAYL 168          
BLAST of EY692791 vs. ExPASy Swiss-Prot
Match: PSB4_HUMAN (Proteasome subunit beta type-4 OS=Homo sapiens GN=PSMB4 PE=1 SV=4)

HSP 1 Score: 136.732 bits (343), Expect = 1.027e-31
Identity = 60/129 (46.51%), Postives = 95/129 (73.64%), Query Frame = 3
Query:  138 PIQRTQLPYVTGTSVVALKYKDGILMAADMGGSYGSTIRYKSVERIKPIGKHSLLGASGEISDFQELSRNLDELILYDNMWDDGNSLGPKELHSYLTRVMYNMRHKLDPLWNSLVLGGVQNGHEYLGVV 524
            PI RTQ P VTGTSV+ +K++ G+++AADM GSYGS  R++++ RI  +   ++LGASG+ +DFQ L + L ++++ + +  DG+S  P+ +HS+LTR MY+ R K++PLWN++V+GG  +G  +LG V
Sbjct:   42 PITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYV 170          

HSP 2 Score: 21.9422 bits (45), Expect = 1.027e-31
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = 2
Query:  527 MIGVNFEDSHVATGFGITL 583
            M+GV +E   +ATG+G  L
Sbjct:  172 MLGVAYEAPSLATGYGAYL 190          
BLAST of EY692791 vs. ExPASy Swiss-Prot
Match: PSB4_MOUSE (Proteasome subunit beta type-4 OS=Mus musculus GN=Psmb4 PE=1 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.267e-31
Identity = 60/129 (46.51%), Postives = 94/129 (72.87%), Query Frame = 3
Query:  138 PIQRTQLPYVTGTSVVALKYKDGILMAADMGGSYGSTIRYKSVERIKPIGKHSLLGASGEISDFQELSRNLDELILYDNMWDDGNSLGPKELHSYLTRVMYNMRHKLDPLWNSLVLGGVQNGHEYLGVV 524
            PI RTQ P VTGTSV+ +K+  G+++AADM GSYGS  R++++ RI  +   ++LGASG+ +DFQ L + L ++++ + +  DG+S  P+ +HS+LTR MY+ R K++PLWN++V+GG  +G  +LG V
Sbjct:   42 PITRTQNPMVTGTSVLGVKFDGGVVIAADMLGSYGSLARFRNISRIMRVNDSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYV 170          

HSP 2 Score: 21.9422 bits (45), Expect = 2.267e-31
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = 2
Query:  527 MIGVNFEDSHVATGFGITL 583
            M+GV +E   +ATG+G  L
Sbjct:  172 MLGVAYEAPSLATGYGAYL 190          
BLAST of EY692791 vs. ExPASy Swiss-Prot
Match: PSB4_RAT (Proteasome subunit beta type-4 OS=Rattus norvegicus GN=Psmb4 PE=1 SV=2)

HSP 1 Score: 135.191 bits (339), Expect = 2.953e-31
Identity = 61/132 (46.21%), Postives = 94/132 (71.21%), Query Frame = 3
Query:  129 PNDPIQRTQLPYVTGTSVVALKYKDGILMAADMGGSYGSTIRYKSVERIKPIGKHSLLGASGEISDFQELSRNLDELILYDNMWDDGNSLGPKELHSYLTRVMYNMRHKLDPLWNSLVLGGVQNGHEYLGVV 524
            P  PI RTQ P VTGTSV+ +K+  G+++AADM GSYGS  R++++ RI  +   ++LGASG+ +DFQ L + L ++++ + ++ DG+S  P+ +HS+LTR MY+ R K++PLWN+ V+GG   G  +LG V
Sbjct:   38 PARPITRTQNPMVTGTSVLGVKFDCGVVIAADMLGSYGSLARFRNISRIMRVNDSTMLGASGDYADFQYLKQVLGQMVIDEELFGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTKVIGGYAGGESFLGYV 169          

HSP 2 Score: 21.9422 bits (45), Expect = 2.953e-31
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = 2
Query:  527 MIGVNFEDSHVATGFGITL 583
            M+GV +E   +ATG+G  L
Sbjct:  171 MLGVAYEAPSLATGYGAYL 189          
BLAST of EY692791 vs. ExPASy Swiss-Prot
Match: PSB4_DICDI (Proteasome subunit beta type-4 OS=Dictyostelium discoideum GN=psmB4-1 PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 4.393e-30
Identity = 62/127 (48.82%), Postives = 91/127 (71.65%), Query Frame = 3
Query:  144 QRTQLPYVTGTSVVALKYKDGILMAADMGGSYGSTIRYKSVERIKPIGKHSLLGASGEISDFQELSRNLDELILYDNMWDDGNSLGPKELHSYLTRVMYNMRHKLDPLWNSLVLGGVQNGHEYLGVV 524
            Q+T  P VTGTSV+A+KY  G++M +DM  SYGS  R+ S ER+K  G ++++GASGE SDFQ ++  L++L+  D+  DDG+ L  +E+ +YL RV+YN R++ +PLWN+LV+ G Q G  +LG V
Sbjct:   35 QKTLDPIVTGTSVIAIKYDKGVVMGSDMLLSYGSLARFNSTERMKKFGGYTIVGASGEYSDFQSITNTLNDLVTDDHCMDDGSKLSSEEIWNYLARVLYNQRNRGNPLWNTLVVMGYQGGKSFLGKV 161          
BLAST of EY692791 vs. ExPASy Swiss-Prot
Match: PSB4_SCHPO (Probable proteasome subunit beta type-4 OS=Schizosaccharomyces pombe GN=SPBC577.10 PE=2 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 3.148e-28
Identity = 57/119 (47.90%), Postives = 87/119 (73.11%), Query Frame = 3
Query:  138 PIQRTQLPYVTGTSVVALKYKDGILMAADMGGSYGSTIRYKSVERIKPIGKHSLLGASGEISDFQELSRNLDELILYDNMWDDGNSLGPKELHSYLTRVMYNMRHKLDPLWNSLVLGGV 494
            PIQRT  P VTG+SV+ALK+ DG+++AAD   SYGS  R+  VER+  +G ++++GA G+ISD+Q++ R L++L + +  + DG +L P  +H YL++V+Y  R+KLDP WN L++ GV
Sbjct:   32 PIQRTVQPIVTGSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQIQRLLEKLEIKEGNYGDGYALQPSYIHEYLSKVLYARRNKLDPYWNQLIVAGV 150          
BLAST of EY692791 vs. ExPASy Swiss-Prot
Match: PSB4_YEAST (Proteasome component PRE4 OS=Saccharomyces cerevisiae GN=PRE4 PE=1 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 4.546e-27
Identity = 61/135 (45.19%), Postives = 93/135 (68.89%), Query Frame = 3
Query:  138 PIQRTQLPYVTGTSVVALKYKDGILMAADMGGSYGSTIRYKSVERIKPIGKHSLLGASGEISDFQELSRNLDELI---LYDN-MWDDGNSLGPKELHSYLTRVMYNMRHKLDPLWNSLVLGGVQ-NGHEYLGVVS 527
            P+  TQ P VTGTSV+++KY +G+++AAD  GSYGS +R+  VER+ P+G ++++G SG+ISD Q + R L +L+    YDN + D   +L P  +  YL  VMY  R K++PLWN++++ GVQ NG ++L  V+
Sbjct:   30 PMVNTQQPIVTGTSVISMKYDNGVIIAADNLGSYGSLLRFNGVERLIPVGDNTVVGISGDISDMQHIERLLKDLVTENAYDNPLADAEEALEPSYIFEYLATVMYQRRSKMNPLWNAIIVAGVQSNGDQFLRYVN 164          
BLAST of EY692791 vs. ExPASy Swiss-Prot
Match: PSB4_PIG (Proteasome subunit beta type-4 (Fragment) OS=Sus scrofa GN=PSMB4 PE=2 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 5.937e-27
Identity = 54/113 (47.79%), Postives = 83/113 (73.45%), Query Frame = 3
Query:  138 PIQRTQLPYVTGTSVVALKYKDGILMAADMGGSYGSTIRYKSVERIKPIGKHSLLGASGEISDFQELSRNLDELILYDNMWDDGNSLGPKELHSYLTRVMYNMRHKLDPLWNS 476
            PI RTQ P VTGTSV+ +K++ G+++AADM GSYGS  R++++ RI  +   ++LGASG+ +DFQ L + L ++++ + +  DG+S  PK +HS+LTR MYN R K++PLW +
Sbjct:   42 PITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPKAIHSWLTRAMYNRRFKMNPLWTT 154          
The following BLAST results are available for this feature:
BLAST of EY692791 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12
Match NameE-valueIdentityDescription
PSB4_ARATH1.516e-5978.91Proteasome subunit beta type-4 OS=Arabidopsis thal... [more]
PSB4_BOVIN2.105e-3248.06Proteasome subunit beta type-4 OS=Bos taurus GN=PS... [more]
PSB4_XENLA2.746e-3245.74Proteasome subunit beta type-4 (Fragment) OS=Xenop... [more]
PSB4_HUMAN1.027e-3146.51Proteasome subunit beta type-4 OS=Homo sapiens GN=... [more]
PSB4_MOUSE2.267e-3146.51Proteasome subunit beta type-4 OS=Mus musculus GN=... [more]
PSB4_RAT2.953e-3146.21Proteasome subunit beta type-4 OS=Rattus norvegicu... [more]
PSB4_DICDI4.393e-3048.82Proteasome subunit beta type-4 OS=Dictyostelium di... [more]
PSB4_SCHPO3.148e-2847.90Probable proteasome subunit beta type-4 OS=Schizos... [more]
PSB4_YEAST4.546e-2745.19Proteasome component PRE4 OS=Saccharomyces cerevis... [more]
PSB4_PIG5.937e-2747.79Proteasome subunit beta type-4 (Fragment) OS=Sus s... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-004-E02-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY692791 ID=EY692791; Name=EY692791; organism=Citrus sinensis; type=EST; length=930bp
CCCACGCGTCCGCTCAGAAGTTAGATCGTGAAGCCTGAGCTGATCAGCTT
TGCTATTTACTGAGAAAGTGAATCAAATCTCAAAACGAATTAATGGAATC
GATCCAAGCCAAACAAGGACTTGTCGGCCCTAACGACCCCATTCAAAGAA
CCCAGTTACCATATGTGACTGGTACATCTGTTGTTGCGCTGAAGTACAAA
GATGGCATATTAATGGCTGCTGACATGGGAGGTTCCTATGGGTCAACAAT
CCGATACAAGAGTGTGGAACGGATTAAACCTATTGGAAAGCATTCTCTAC
TGGGTGCCAGTGGAGAAATAAGTGATTTTCAGGAGCTTTCGCGTAATCTT
GATGAGCTCATACTTTATGACAATATGTGGGATGATGGGAACTCTTTGGG
GCCAAAAGAGTTGCACAGCTATTTGACCAGGGTCATGTATAACATGCGTC
ACAAGTTAGATCCATTGTGGAACTCTCTTGTGCTTGGTGGAGTGCAAAAT
GGACACGAATATCTTGGCGTGGTATCATGATAGGAGTGAACTTTGAGGAC
AGCCATGTTGCAACTGGTTTTGGGATCACTTTGCAAGGCAATTTTCCTGA
TGAATGCATGAAAACTTGATTATGAATATTGCCTAAATCGCTGAAAAGTG
CATGCAGAGCACCTGATCTTGAAGATCTGCCGTCAACAGCTGCTATACAA
AGATCACTGGGAAGGGCACAATTTCCAATCCTCGCATGAATACTTTTGGG
ATTACTGAATTCAAATCAAAAGGGTGCTTAGGACATGGAATGGACATGAA
AACTTCTAAAGTTTTTTCTGGGGACTTGGAGAGAACTTGTTCTGCTTTTC
CCTGATATTGGGAATAAGAACAAAGGTTATAAATCTCGGCTTATTTGTTT
AACTTTACTTAAGGCCACTGAACAAGTAAA
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