EY692487

Overview
NameEY692487
Unique NameEY692487
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length912
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY692487 vs. ExPASy Swiss-Prot
Match: FTSH2_ARATH (Cell division protease ftsH homolog 2, chloroplastic OS=Arabidopsis thaliana GN=FTSH2 PE=1 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 1.728e-63
Identity = 158/285 (55.44%), Postives = 179/285 (62.81%), Query Frame = 3
Query:   57 MAASSACLVGNILTTHKT-KTNLSKDFHGRHLLFNSSFRSLEKKPKVAVVKASLS-QKQHEGRRGFLKKLLGNVGVGTALLGSGKAYADEQGVSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQSK----LNSRWNQYWCDI**XCPE**GKTDFMEVVESLRSPRDY-SYWSSYSQGVXSRGPSENWETLL 890
            MAASSACLVGN L+ + T K  LSK F GR     +SF S+ +  KV VVKASL  +K+ EGRR FLK LLGN GVG  L+ SGKA ADEQGVSSSRMSYSRFLEYLDKDRV KVDL+ENGTIAIVEA+SPELGNRV+RVRVQLPGLSQELLQK R KNIDFAAHNAQED GS+LFNLIGN                                   QSK    +       + D+         K DFMEVVE L+ P  + +  +   +GV   GP    +TLL
Sbjct:    1 MAASSACLVGNGLSVNTTTKQRLSKHFSGRQ----TSFSSVIRTSKVNVVKASLDGKKKQEGRRDFLKILLGNAGVG--LVASGKANADEQGVSSSRMSYSRFLEYLDKDRVNKVDLYENGTIAIVEAVSPELGNRVERVRVQLPGLSQELLQKLRAKNIDFAAHNAQEDQGSVLFNLIGNLAFPALLIGGLFLLSRRSGGGMGGPGGPGNPLQFGQSKAKFQMEPNTGVTFDDV---AGVDEAKQDFMEVVEFLKKPERFTAVGAKIPKGVLLIGPPGTGKTLL 276          
BLAST of EY692487 vs. ExPASy Swiss-Prot
Match: FTSH2_ORYSJ (Cell division protease ftsH homolog 2, chloroplastic OS=Oryza sativa subsp. japonica GN=FTSH2 PE=3 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 4.088e-61
Identity = 110/132 (83.33%), Postives = 117/132 (88.64%), Query Frame = 3
Query:  201 VKASLSQKQHEGRRGFLKKLLGNVGVGT-ALLGSGKAYADEQGVSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIGN 593
            V ASL  K ++ RR FLK  LGN+GVG   LLG+ +A A+EQGVSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQK REKNIDFAAH+ QEDSGSLLFNLIGN
Sbjct:   31 VTASLEHKTNDARRKFLKLALGNLGVGLPTLLGAKRALAEEQGVSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKLREKNIDFAAHSNQEDSGSLLFNLIGN 162          

HSP 2 Score: 50.447 bits (119), Expect = 4.088e-61
Identity = 35/64 (54.69%), Postives = 40/64 (62.50%), Query Frame = 1
Query:  727 NTGVTFDXCARSDEAKQISWKWWSPLEARE-ITAIGARIPKVXXLVVPQKTGR-LSCQAIAGEA 912
            NTGVTFD  A  DEAKQ   +    L+  E  TA+GARIPK   LV P  TG+ L  +AIAGEA
Sbjct:  208 NTGVTFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLVGPPGTGKTLLAKAIAGEA 271          

HSP 3 Score: 20.7866 bits (42), Expect = 4.088e-61
Identity = 9/16 (56.25%), Postives = 10/16 (62.50%), Query Frame = 2
Query:  680 FPPCLWSVKAKFQMEP 727
            FP      +AKFQMEP
Sbjct:  192 FPLGFGQSRAKFQMEP 207          
BLAST of EY692487 vs. ExPASy Swiss-Prot
Match: FTSH8_ARATH (Cell division protease ftsH homolog 8, chloroplastic OS=Arabidopsis thaliana GN=FTSH8 PE=1 SV=1)

HSP 1 Score: 230.72 bits (587), Expect = 8.876e-60
Identity = 146/285 (51.23%), Postives = 167/285 (58.60%), Query Frame = 3
Query:   57 MAASSACLVGNILTTHKTKTNLSKDFHGRHLLFNSSFRSLEKKPKVAVVKASLSQKQHEGRRGFLKKLLGNVGVGTALLGSGKAYADEQG--VSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQSK----LNSRWNQYWCDI**XCPE**GKTDFMEVVESLRSPRDY-SYWSSYSQGVXSRGPSENWETLL 890
            MAASSACL+GN L+ + TK    K               L++  KV VVKASL +K+HEGRRGF K LLGN   G  LL SG A ADEQG  VSSSRMSYSRFLEYLDK RV+KVDL+ENGTIAIVEA+SPELGNR+QRVRVQLPGLSQELLQK R KNIDFAAHNAQED GS + NLIGN                                   QSK    +       + D+         K DFMEVVE L+ P  + +  +   +GV   GP    +TLL
Sbjct:    1 MAASSACLLGNGLSVYTTKQRFQK-------------LGLDRTSKVTVVKASLDEKKHEGRRGFFKLLLGNAAAGVGLLASGNANADEQGQGVSSSRMSYSRFLEYLDKGRVEKVDLYENGTIAIVEAVSPELGNRIQRVRVQLPGLSQELLQKLRAKNIDFAAHNAQEDQGSPILNLIGNLAFPVILIGGLFLLSRRSSGGMGGPGGPGFPLQIGQSKAKFQMEPNTGVTFDDV---AGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLVGPPGTGKTLL 269          
BLAST of EY692487 vs. ExPASy Swiss-Prot
Match: FTSH6_ORYSJ (Cell division protease ftsH homolog 6, chloroplastic OS=Oryza sativa subsp. japonica GN=FTSH6 PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 2.632e-30
Identity = 62/145 (42.76%), Postives = 87/145 (60.00%), Query Frame = 3
Query:  168 RSLEKKPKVAVVKASLSQKQHEGRRGFLKKLLGNVGVGTALLGSGKAYADE---QGVSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIGN 593
            R+     K+    +S S+     RR  L+     +G+G  L  +  A A+    + V+S+RMSYSRFLEYLD   VKKVD FENGT+A+ E       +RV RV+VQLPGL  EL++K R+K +DFAAH  +  +G +L +L+ N
Sbjct:   30 RTTPPPAKITPPSSSSSEAAGLSRRRLLQS--AGLGLGLGLTAARPARAEATAPEEVTSNRMSYSRFLEYLDAGAVKKVDFFENGTVAVAEVDDAAALSRVHRVKVQLPGLPAELVRKLRDKGVDFAAHPVEPSAGVMLLDLLVN 172          

HSP 2 Score: 45.4394 bits (106), Expect = 2.632e-30
Identity = 33/63 (52.38%), Postives = 38/63 (60.32%), Query Frame = 1
Query:  730 TGVTFDXCARSDEAKQISWKWWSPLEARE-ITAIGARIPKVXXLVVPQKTGR-LSCQAIAGEA 912
            TGVTFD  A  DEAKQ   +    L+  E  TA+GAR PK   LV P  TG+ L  +AIAGEA
Sbjct:  216 TGVTFDDVAGVDEAKQDFQEIVQFLKFPEKFTAVGARTPKGVLLVGPPGTGKTLLAKAIAGEA 278          

HSP 3 Score: 20.7866 bits (42), Expect = 2.632e-30
Identity = 8/8 (100.00%), Postives = 8/8 (100.00%), Query Frame = 2
Query:  704 KAKFQMEP 727
            KAKFQMEP
Sbjct:  207 KAKFQMEP 214          
BLAST of EY692487 vs. ExPASy Swiss-Prot
Match: FTSH6_ARATH (Cell division protease ftsH homolog 6, chloroplastic OS=Arabidopsis thaliana GN=FTSH6 PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.637e-21
Identity = 89/288 (30.90%), Postives = 129/288 (44.79%), Query Frame = 3
Query:   54 KMAASSACL---VGNILTTHKTKTNLSKDFHGRHLLFNSSFRSLEKKPKVAVVKASLSQKQHEGRRGFLKKLLGNVG----VGTALLGSGKAYADEQ-GVSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQSKLNSRWNQYWCDI**XCPE**GKTDFMEVVESLRSPRDYS-YWSSYSQGVXSRGPSENWETLL 890
            KMA+SS+ L   + NI T  K      K              SL K               H+  +  L  L   +G    +GT L    KA  +     +S+RMSYSRFL++L ++ VKKVDL ENGT+AIVE  +P +G ++QRVRV LPGL  +L+++ +EKN+DFAAH    + G+ L N +GN                                   + ++       + D+         K DF E+VE L++P  +S   +   +GV   GP    +TLL
Sbjct:    2 KMASSSSALSFPLSNIPTCSKKSQQFQKP------------ASLSKSSHTHKPSLKTQILHHKFTKRNLLSLTTALGFTSALGTVLAHPAKAEPEAPIEATSNRMSYSRFLQHLKENEVKKVDLIENGTVAIVEISNPVVG-KIQRVRVNLPGLPVDLVREMKEKNVDFAAHPMNVNWGAFLLNFLGNLGFPLILLVSLLLTSSSRRNPAGPNLPFGLGRSKAKFQMEPNTGITFEDV---AGVDEAKQDFEEIVEFLKTPEKFSALGAKIPKGVLLTGPPGTGKTLL 273          
BLAST of EY692487 vs. ExPASy Swiss-Prot
Match: FTSH_PORPU (Cell division protease ftsH homolog OS=Porphyra purpurea GN=ftsH PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.809e-20
Identity = 52/93 (55.91%), Postives = 66/93 (70.97%), Query Frame = 3
Query:  327 VSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDS------GSLLFNLI 587
            ++SSRM+Y RFLEYLD   VK+VDL+EN   AIVEA+ PELGNRVQR+RV+LP  + EL+ K R+ N+D  AH  +  S      G+LLF LI
Sbjct:   38 IASSRMTYGRFLEYLDMGWVKRVDLYENNHTAIVEAVGPELGNRVQRIRVELPASAPELITKLRKANVDLDAHPPKSTSAVWGLLGNLLFPLI 130          
BLAST of EY692487 vs. ExPASy Swiss-Prot
Match: FTSH_GUITH (Cell division protease ftsH homolog OS=Guillardia theta GN=ftsH PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 2.363e-20
Identity = 51/91 (56.04%), Postives = 66/91 (72.53%), Query Frame = 3
Query:  321 QGVSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAH--NAQEDSGSLLFNLI 587
            + ++SSRM+Y RFLEYLD   VKKVDL++ G  AIVEAI PELGNR+QR+RV+LP  + EL+ K R+ N+D  AH  N    + SL+ NLI
Sbjct:   36 KNIASSRMTYGRFLEYLDLGWVKKVDLYDEGHTAIVEAIGPELGNRIQRIRVELPATAPELITKLRKANVDLDAHATNDSTPAWSLIGNLI 126          
BLAST of EY692487 vs. ExPASy Swiss-Prot
Match: FSTH_PORYE (Cell division protease ftsH homolog OS=Porphyra yezoensis GN=ftsH PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 2.363e-20
Identity = 50/89 (56.18%), Postives = 66/89 (74.16%), Query Frame = 3
Query:  327 VSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIGN 593
            ++SSRM+Y RFLEYLD   VK+VDL+EN   AIVEA+ PELGNRVQR+RV+LP  + EL+ K R+ N+D  AH  +  S S ++ L+GN
Sbjct:   38 IASSRMTYGRFLEYLDMGWVKRVDLYENNHTAIVEAVGPELGNRVQRIRVELPASAPELITKLRKANVDLDAHPPK--STSAVWGLLGN 124          
BLAST of EY692487 vs. ExPASy Swiss-Prot
Match: FTSH_ODOSI (Cell division protease ftsH homolog OS=Odontella sinensis GN=ftsH PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 9.293e-17
Identity = 44/89 (49.44%), Postives = 64/89 (71.91%), Query Frame = 3
Query:  327 VSSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSGSLLFNLIGN 593
            V SS+M+Y RFLEYL+   V +VDL++N   AIV+A SPELGNR Q +RV++P  + +L+QK +E NIDF AH A++   ++  N++ N
Sbjct:   50 VISSKMTYGRFLEYLEMGWVNQVDLYDNSRNAIVQASSPELGNRPQTIRVEIPVGASQLIQKLKEYNIDFDAHPAEQK--NIFVNILSN 136          
BLAST of EY692487 vs. ExPASy Swiss-Prot
Match: FTSH_CYACA (Cell division protease ftsH homolog OS=Cyanidium caldarium GN=ftsH PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 5.099e-15
Identity = 44/88 (50.00%), Postives = 59/88 (67.05%), Query Frame = 3
Query:  330 SSSRMSYSRFLEYLDKDRVKKVDLFENGTIAIVEAISPELGNRVQRVRVQLPGLSQELLQKFREKNIDFAAHNAQEDSG--SLLFNLI 587
            SSSRM+Y RFLEY+D + VKKVDL++N   AIV+ I+P++    Q VRV+LP  S EL+ K + K IDF AH +  +    S L NL+
Sbjct:   37 SSSRMTYGRFLEYIDMNWVKKVDLYDNARTAIVDIINPDIKGEEQLVRVELPTFSSELVSKLKNKLIDFDAHPSSSNVNLVSWLSNLL 124          
The following BLAST results are available for this feature:
BLAST of EY692487 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11
Match NameE-valueIdentityDescription
FTSH2_ARATH1.728e-6355.44Cell division protease ftsH homolog 2, chloroplast... [more]
FTSH2_ORYSJ4.088e-6183.33Cell division protease ftsH homolog 2, chloroplast... [more]
FTSH8_ARATH8.876e-6051.23Cell division protease ftsH homolog 8, chloroplast... [more]
FTSH6_ORYSJ2.632e-3042.76Cell division protease ftsH homolog 6, chloroplast... [more]
FTSH6_ARATH1.637e-2130.90Cell division protease ftsH homolog 6, chloroplast... [more]
FTSH_PORPU1.809e-2055.91Cell division protease ftsH homolog OS=Porphyra pu... [more]
FTSH_GUITH2.363e-2056.04Cell division protease ftsH homolog OS=Guillardia ... [more]
FSTH_PORYE2.363e-2056.18Cell division protease ftsH homolog OS=Porphyra ye... [more]
FTSH_ODOSI9.293e-1749.44Cell division protease ftsH homolog OS=Odontella s... [more]
FTSH_CYACA5.099e-1550.00Cell division protease ftsH homolog OS=Cyanidium c... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-001-B10-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY692487 ID=EY692487; Name=EY692487; organism=Citrus sinensis; type=EST; length=912bp
CCACGCGTCCGCGGACGCGTGGGTCAAATTCTGTTCCAACAATTCTTTCT
TTCAAGATGGCAGCTTCATCAGCTTGCCTTGTAGGGAATATTTTGACAAC
ACACAAGACTAAAACAAATTTGAGCAAGGACTTCCATGGTAGGCATCTTC
TGTTTAACTCGAGTTTTCGATCATTGGAAAAGAAGCCAAAAGTAGCCGTT
GTAAAGGCATCTTTGAGCCAGAAGCAGCATGAAGGAAGAAGAGGGTTTCT
TAAGAAGCTGCTTGGAAATGTTGGAGTTGGTACTGCTTTGTTGGGGAGTG
GGAAAGCCTATGCCGATGAACAGGGGGTTTCTTCCTCTAGGATGTCTTAC
TCTAGGTTCTTGGAGTATCTTGATAAGGACAGGGTGAAGAAGGTTGATTT
GTTTGAGAATGGTACCATAGCTATTGTGGAGGCTATTTCCCCTGAGTTGG
GTAACCGGGTGCAACGAGTTCGCGTTCAACTTCCAGGTCTAAGTCAGGAG
CTTCTCCAGAAATTCAGGGAGAAGAACATTGATTTTGCTGCCCATAATGC
TCAAGAGGACTCAGGATCATTGTTGTTTAACTTGATTGGGAATCTGGCTT
TCCCACTTATCTTAATTGGAGGGCTGTTCCTTCTCTCAAGGCGTTCATCT
GGTGGAATGGGCGGTCCTGGTGGGCCTGGTTTCCCCCTTGCCTTTGGTCA
GTCAAAGCTAAATTCCAGATGGAACCAATACTGGTGTGACATTTGATGAN
TGTGCCCGGAGTGATGAGGCAAAACAGATTTCATGGAAGTGGTGGAGTCC
CTTAGAAGCCCGAGAGATTACAGCTATTGGAGCTCGTATTCCCAAGGTGT
TNNCTCTCGTGGTCCCTCAGAAAACTGGGAGACTCTCTTGCCAGGCAATT
GCTGGTGAAGCC
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