EY711423

Overview
NameEY711423
Unique NameEY711423
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length884
Libraries
Library NameType
Sweet orange fruit, development stadium (3 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY711423 vs. ExPASy Swiss-Prot
Match: PMM_SOYBN (Phosphomannomutase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 458.373 bits (1178), Expect = 2.491e-128
Identity = 220/245 (89.80%), Postives = 236/245 (96.33%), Query Frame = 3
Query:   18 MAARKQGLLALFDVDGTLTAPRKAATPQMLEFMRELRKVVTVGVVGGSDLSKISEQLGKTVIDEYDYVFSENGLVAHKDGKLIGTQSLKSFLGGEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNISPIGRNCSQEERDEFERYDKIHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLDDFNEIHFFGDKTYKGGNDHEIFESERTVGHTVTSPEDTMEKCKALFL 752
            MAAR+ GL+ALFDVDGTLTAPRK  TP+ML FM+ELRKVVTVGVVGGSDL KISEQLG TV ++YDYVFSENGLVAHK+GKLIGTQSLKSFLG EKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLN+SPIGRNCSQEERDEFE+YDK+HNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLD FNEIHFFGDKTYKGGNDHEI+ESERTVGHTVTSP+DT+++CK+LFL
Sbjct:    1 MAARRPGLIALFDVDGTLTAPRKVVTPEMLTFMQELRKVVTVGVVGGSDLIKISEQLGSTVTNDYDYVFSENGLVAHKEGKLIGTQSLKSFLGEEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNVSPIGRNCSQEERDEFEKYDKVHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLDGFNEIHFFGDKTYKGGNDHEIYESERTVGHTVTSPDDTVKQCKSLFL 245          
BLAST of EY711423 vs. ExPASy Swiss-Prot
Match: PMM_TOBAC (Phosphomannomutase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 449.514 bits (1155), Expect = 1.157e-125
Identity = 217/247 (87.85%), Postives = 232/247 (93.93%), Query Frame = 3
Query:   18 MAARKQGLLALFDVDGTLTAPRKAATPQMLEFMRELRKVVTVGVVGGSDLSKISEQLGKTVIDEYDYVFSENGLVAHKDGKLIGTQSLKSFLGGEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNISPIGRNCSQEERDEFERYDKIHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLDDFNEIHFFGDKTYKGGNDHEIFESERTVGHTVTSPEDTMEKCKALFLAK 758
            MAARK GL+ALFDVDGTLTAPRK  TP+ML+FM+ELRKVVTVGVVGGSDL KISEQLGKTV  +YDY FSENGLVAHKDGKLIGTQSLKSFLG EKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLN+SPIGR+CSQEERDEFE+YDK+H IR  MVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYL++FNEIHFFGDKTYKGGNDHEI+ESERTVGHTVTSPEDT+++C   FL K
Sbjct:    1 MAARKPGLIALFDVDGTLTAPRKEVTPEMLKFMKELRKVVTVGVVGGSDLVKISEQLGKTVTTDYDYCFSENGLVAHKDGKLIGTQSLKSFLGDEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNVSPIGRDCSQEERDEFEKYDKVHKIRQTMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLEEFNEIHFFGDKTYKGGNDHEIYESERTVGHTVTSPEDTVKQCSEQFLGK 247          
BLAST of EY711423 vs. ExPASy Swiss-Prot
Match: PMM_SOLLC (Phosphomannomutase OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 445.277 bits (1144), Expect = 2.183e-124
Identity = 215/247 (87.04%), Postives = 231/247 (93.52%), Query Frame = 3
Query:   18 MAARKQGLLALFDVDGTLTAPRKAATPQMLEFMRELRKVVTVGVVGGSDLSKISEQLGKTVIDEYDYVFSENGLVAHKDGKLIGTQSLKSFLGGEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNISPIGRNCSQEERDEFERYDKIHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLDDFNEIHFFGDKTYKGGNDHEIFESERTVGHTVTSPEDTMEKCKALFLAK 758
            MAARK GL+ALFDVDGTLTAPRK +TPQML+FM+ELRKVVTVGVVGGSDL KISEQLG TV ++YDYVFSENGLVAHKDGKLIG QSLKS LG EKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLN+SPIGRNCSQEERDEFE+YDK+  IR  MVSVLREKFAH NLTFSIGGQISFDVFPQGWDKTYCLRYL++FNEIHFFGDKTYKGGNDHEI+ESERTVGHTVTSPE+T+++C  LFL K
Sbjct:    1 MAARKAGLIALFDVDGTLTAPRKESTPQMLKFMQELRKVVTVGVVGGSDLVKISEQLGNTVTNDYDYVFSENGLVAHKDGKLIGKQSLKSHLGDEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNVSPIGRNCSQEERDEFEKYDKVQKIRETMVSVLREKFAHFNLTFSIGGQISFDVFPQGWDKTYCLRYLEEFNEIHFFGDKTYKGGNDHEIYESERTVGHTVTSPEETLKQCSVLFLGK 247          
BLAST of EY711423 vs. ExPASy Swiss-Prot
Match: PMM_WHEAT (Phosphomannomutase OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 442.195 bits (1136), Expect = 1.848e-123
Identity = 210/247 (85.02%), Postives = 236/247 (95.55%), Query Frame = 3
Query:   21 AARKQ-GLLALFDVDGTLTAPRKAATPQMLEFMRELRKVVTVGVVGGSDLSKISEQLGKTVIDEYDYVFSENGLVAHKDGKLIGTQSLKSFLGGEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNISPIGRNCSQEERDEFERYDKIHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLDDFNEIHFFGDKTYKGGNDHEIFESERTVGHTVTSPEDTMEKCKALFLAK 758
            AARK  G+LALFDVDGTLTAPRK  TP+MLEFM+ LR+ VTVGVVGGSDL KISEQLGK+VI +YDYVFSENGLVAHKDGKLIGTQSLK++LG ++LKEFINFTLHYIADLDIPIKRGTFIEFRSGM+N+SPIGRNCSQEERD+FE+YDK+HN+RPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYL++F EIHFFGDKTYKGGNDHEIFES+RTVGHTVTSP DT+++CK++FL++
Sbjct:    3 AARKDAGVLALFDVDGTLTAPRKEVTPEMLEFMKRLRENVTVGVVGGSDLVKISEQLGKSVITDYDYVFSENGLVAHKDGKLIGTQSLKTYLGDDQLKEFINFTLHYIADLDIPIKRGTFIEFRSGMINVSPIGRNCSQEERDDFEKYDKVHNVRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLEEFKEIHFFGDKTYKGGNDHEIFESDRTVGHTVTSPNDTVQQCKSIFLSE 249          
BLAST of EY711423 vs. ExPASy Swiss-Prot
Match: PMM_ORYSJ (Phosphomannomutase OS=Oryza sativa subsp. japonica GN=PMM PE=2 SV=2)

HSP 1 Score: 440.269 bits (1131), Expect = 7.021e-123
Identity = 210/248 (84.68%), Postives = 236/248 (95.16%), Query Frame = 3
Query:   18 MAARKQ-GLLALFDVDGTLTAPRKAATPQMLEFMRELRKVVTVGVVGGSDLSKISEQLGKTVIDEYDYVFSENGLVAHKDGKLIGTQSLKSFLGGEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNISPIGRNCSQEERDEFERYDKIHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLDDFNEIHFFGDKTYKGGNDHEIFESERTVGHTVTSPEDTMEKCKALFLAK 758
            MAARK  G+LALFDVDGTLTAPRK  TP+ML+FM++LR+ VTVGVVGGSDL KISEQLGK+V  +YDY FSENGLVAHK+G+LIGTQSLKSFLG ++LKEFINFTLHYIADLDIPIKRGTFIEFRSGMLN+SPIGRNCSQEERDEFE+YDK+HNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYL++F EIHFFGDKTYKGGND+EIFES+RT+GHTVTSP+DT E+C++LF++K
Sbjct:    1 MAARKNAGVLALFDVDGTLTAPRKVVTPEMLQFMKQLREHVTVGVVGGSDLVKISEQLGKSVTTDYDYCFSENGLVAHKNGELIGTQSLKSFLGDDQLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNVSPIGRNCSQEERDEFEKYDKVHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLEEFQEIHFFGDKTYKGGNDYEIFESDRTIGHTVTSPDDTAEQCRSLFMSK 248          
BLAST of EY711423 vs. ExPASy Swiss-Prot
Match: PMM_ORYSI (Phosphomannomutase OS=Oryza sativa subsp. indica GN=PMM PE=3 SV=1)

HSP 1 Score: 440.269 bits (1131), Expect = 7.021e-123
Identity = 210/248 (84.68%), Postives = 236/248 (95.16%), Query Frame = 3
Query:   18 MAARKQ-GLLALFDVDGTLTAPRKAATPQMLEFMRELRKVVTVGVVGGSDLSKISEQLGKTVIDEYDYVFSENGLVAHKDGKLIGTQSLKSFLGGEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNISPIGRNCSQEERDEFERYDKIHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLDDFNEIHFFGDKTYKGGNDHEIFESERTVGHTVTSPEDTMEKCKALFLAK 758
            MAARK  G+LALFDVDGTLTAPRK  TP+ML+FM++LR+ VTVGVVGGSDL KISEQLGK+V  +YDY FSENGLVAHK+G+LIGTQSLKSFLG ++LKEFINFTLHYIADLDIPIKRGTFIEFRSGMLN+SPIGRNCSQEERDEFE+YDK+HNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYL++F EIHFFGDKTYKGGND+EIFES+RT+GHTVTSP+DT E+C++LF++K
Sbjct:    1 MAARKNAGVLALFDVDGTLTAPRKVVTPEMLQFMKQLREHVTVGVVGGSDLVKISEQLGKSVTTDYDYCFSENGLVAHKNGELIGTQSLKSFLGDDQLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNVSPIGRNCSQEERDEFEKYDKVHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLEEFQEIHFFGDKTYKGGNDYEIFESDRTIGHTVTSPDDTAEQCRSLFMSK 248          
BLAST of EY711423 vs. ExPASy Swiss-Prot
Match: PMM_ARATH (Phosphomannomutase OS=Arabidopsis thaliana GN=PMM PE=1 SV=1)

HSP 1 Score: 426.787 bits (1096), Expect = 8.033e-119
Identity = 201/246 (81.71%), Postives = 231/246 (93.90%), Query Frame = 3
Query:   18 MAARKQGLLALFDVDGTLTAPRKAATPQMLEFMRELRKVVTVGVVGGSDLSKISEQLGKTVIDEYDYVFSENGLVAHKDGKLIGTQSLKSFLGGEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNISPIGRNCSQEERDEFERYDKIHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYLDDFNEIHFFGDKTYKGGNDHEIFESERTVGHTVTSPEDTMEKCKALFLA 755
            MAA+  G++ALFDVDGTLTAPRK ATP++L+F+RELRKVVT+GVVGGSDLSKISEQLGKTV ++YDY FSENGLVAHKDGK IG QSLK  LG +KLKE INFTLHYIADLDIPIKRGTFIEFR+GMLN+SPIGRNCSQEERDEFERYDK+ NIRPKMV+ LRE+FAHLNLTFSIGGQISFDVFP+GWDKTYCL+YL+DF+EIHFFGDKTY+GGND+EI+ES +T+GH+VTSP+DT+ KCKALF++
Sbjct:    1 MAAKIPGVIALFDVDGTLTAPRKEATPELLDFIRELRKVVTIGVVGGSDLSKISEQLGKTVTNDYDYCFSENGLVAHKDGKSIGIQSLKLHLGDDKLKELINFTLHYIADLDIPIKRGTFIEFRNGMLNVSPIGRNCSQEERDEFERYDKVQNIRPKMVAELRERFAHLNLTFSIGGQISFDVFPKGWDKTYCLQYLEDFSEIHFFGDKTYEGGNDYEIYESPKTIGHSVTSPDDTVAKCKALFMS 246          
BLAST of EY711423 vs. ExPASy Swiss-Prot
Match: PMM1_DICDI (Phosphomannomutase 1 OS=Dictyostelium discoideum GN=pmmA PE=3 SV=1)

HSP 1 Score: 316.235 bits (809), Expect = 1.529e-85
Identity = 154/239 (64.44%), Postives = 186/239 (77.82%), Query Frame = 3
Query:   42 LALFDVDGTLTAPRKAATPQMLEFMRELRKVVTVGVVGGSDLSKISEQLGKTVIDEYDYVFSENGLVAHKDGKLIGTQSLKSFLGGEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNISPIGRNCSQEERDEFERYDKIHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYL--DDFNEIHFFGDKTYKGGNDHEIFESER-TVGHTVTSPEDTMEKCKALF 749
            + LFDVDGTLT PR   TP+M +F+  LR  V +GVVGGSD++KI EQLG+  I+E+ Y+F+ENGL++ KDG L+ TQ +K FLG E +K+FINF LHYIADLDIPIKRGTF+EFR+GM+NISPIGRNCSQ+ER+EFE+YD  H IRP MVS+L+EKF  L L +SIGGQISFDVFP GWDKTYCLR+L  D +  I+FFGDKT+ GGND+EI    R T   TVTSP DT      LF
Sbjct:    8 ICLFDVDGTLTKPRNVITPEMKDFLAGLRTKVELGVVGGSDINKIKEQLGENCINEFHYLFAENGLLSFKDGSLLATQDIKKFLGEENIKKFINFVLHYIADLDIPIKRGTFVEFRNGMINISPIGRNCSQQEREEFEKYDLEHKIRPTMVSILKEKFQSLGLQYSIGGQISFDVFPIGWDKTYCLRHLPEDKYKTIYFFGDKTFVGGNDYEIANDPRITKSFTVTSPTDTKNFLSELF 246          
BLAST of EY711423 vs. ExPASy Swiss-Prot
Match: PMM_SCHPO (Phosphomannomutase OS=Schizosaccharomyces pombe GN=pmm1 PE=2 SV=1)

HSP 1 Score: 315.464 bits (807), Expect = 2.608e-85
Identity = 156/254 (61.42%), Postives = 197/254 (77.56%), Query Frame = 3
Query:    6 ALAPMAARK-QGLLALFDVDGTLTAPRKAATPQMLEFMRELRKVVTVGVVGGSDLSKISEQL---GKTVIDEYDYVFSENGLVAHKDGKLIGTQSLKSFLGGEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNISPIGRNCSQEERDEFERYDKIHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYL--DDFNEIHFFGDKTYKGGNDHEIFESERTVGHTVTSPEDTMEKCKALF 749
            A+ P+  RK    L LFDVDGTLT  R + +P+MLE ++ LRKVV +G VGGSDLSK  EQL   G+ VID +DY F+ENGL A++ G+ + +QS  ++LG EK ++ +NF LHYIADLDIP+KRGTFIEFR+GM+NISP+GRN + EER+EFER+DK   IR  MV VLREKF    LTFSIGGQISFDVFP GWDKTYCL+++  + F+ IHFFGDKTYKGGND+EIF   RT+GH+VT+P+DT+ + K +F
Sbjct:    2 AVIPIEERKFPKTLVLFDVDGTLTPARLSVSPEMLETLQNLRKVVAIGFVGGSDLSKQQEQLSVNGENVIDSFDYAFAENGLTAYRYGQQLASQSFIAWLGEEKYQKLVNFCLHYIADLDIPVKRGTFIEFRNGMINISPVGRNANTEERNEFERFDKGRKIRATMVDVLREKFKDYGLTFSIGGQISFDVFPAGWDKTYCLQHVEKEGFDTIHFFGDKTYKGGNDYEIFVDPRTIGHSVTNPDDTIAELKKIF 255          
BLAST of EY711423 vs. ExPASy Swiss-Prot
Match: PMM2_MACFA (Phosphomannomutase 2 OS=Macaca fascicularis GN=PMM2 PE=2 SV=1)

HSP 1 Score: 302.753 bits (774), Expect = 1.750e-81
Identity = 147/238 (61.76%), Postives = 185/238 (77.73%), Query Frame = 3
Query:   42 LALFDVDGTLTAPRKAATPQMLEFMRELRKVVTVGVVGGSDLSKISEQLGKTVIDEYDYVFSENGLVAHKDGKLIGTQSLKSFLGGEKLKEFINFTLHYIADLDIPIKRGTFIEFRSGMLNISPIGRNCSQEERDEFERYDKIHNIRPKMVSVLREKFAHLNLTFSIGGQISFDVFPQGWDKTYCLRYL--DDFNEIHFFGDKTYKGGNDHEIFESERTVGHTVTSPEDTMEKCKALF 749
            L LFDVDGTLTAPR+  T +M +F+++LR+ + +GVVGGSD  K+ EQLG  V+++YDYVF ENGLVA+KDGKL+  Q+++S LG   +++ IN+ L YIA + +P KRGTFIEFR+GMLN+SPIGR+CSQEER EF   DK  NIR K V+ LR++FA   LTFSIGGQISFDVFP GWDK YCLR++  D +  I+FFGDKT  GGNDHEIF   RTVG++VT+PEDT   C+ LF
Sbjct:    8 LCLFDVDGTLTAPRQKITKEMDDFLQKLRQKIKIGVVGGSDFEKVQEQLGNDVVEKYDYVFPENGLVAYKDGKLLCKQNIQSHLGEALIQDLINYCLSYIAKVKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFYELDKKENIRQKFVADLRKEFAGKGLTFSIGGQISFDVFPDGWDKRYCLRHVENDGYKTIYFFGDKTMPGGNDHEIFTDPRTVGYSVTAPEDTRRICEELF 245          
The following BLAST results are available for this feature:
BLAST of EY711423 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 22
Match NameE-valueIdentityDescription
PMM_SOYBN2.491e-12889.80Phosphomannomutase OS=Glycine max PE=2 SV=1[more]
PMM_TOBAC1.157e-12587.85Phosphomannomutase OS=Nicotiana tabacum PE=2 SV=1[more]
PMM_SOLLC2.183e-12487.04Phosphomannomutase OS=Solanum lycopersicum PE=2 SV... [more]
PMM_WHEAT1.848e-12385.02Phosphomannomutase OS=Triticum aestivum PE=2 SV=1[more]
PMM_ORYSJ7.021e-12384.68Phosphomannomutase OS=Oryza sativa subsp. japonica... [more]
PMM_ORYSI7.021e-12384.68Phosphomannomutase OS=Oryza sativa subsp. indica G... [more]
PMM_ARATH8.033e-11981.71Phosphomannomutase OS=Arabidopsis thaliana GN=PMM ... [more]
PMM1_DICDI1.529e-8564.44Phosphomannomutase 1 OS=Dictyostelium discoideum G... [more]
PMM_SCHPO2.608e-8561.42Phosphomannomutase OS=Schizosaccharomyces pombe GN... [more]
PMM2_MACFA1.750e-8161.76Phosphomannomutase 2 OS=Macaca fascicularis GN=PMM... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-702-007-E09-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY711423 ID=EY711423; Name=EY711423; organism=Citrus sinensis; type=EST; length=884bp
CGGACGCGTTGGCTCCAATGGCTGCTAGGAAGCAAGGTTTATTAGCTCTC
TTTGATGTTGACGGAACTCTCACGGCTCCGAGAAAGGCGGCAACACCACA
AATGTTAGAGTTCATGCGGGAACTGAGAAAGGTTGTTACAGTTGGAGTCG
TCGGTGGATCTGATCTTTCTAAGATATCAGAGCAGCTTGGGAAGACAGTT
ATTGATGAATACGATTATGTGTTTTCTGAGAATGGTCTTGTGGCTCATAA
GGATGGGAAATTAATTGGCACACAGAGCTTGAAATCATTTCTTGGAGGCG
AAAAGCTCAAGGAATTCATAAATTTTACTCTTCATTACATTGCTGACTTG
GACATTCCAATTAAAAGGGGAACTTTCATTGAATTCCGAAGTGGGATGCT
TAATATTTCACCAATTGGGCGAAACTGCAGCCAAGAAGAAAGGGACGAGT
TTGAGAGGTATGACAAGATTCACAATATACGGCCAAAAATGGTATCTGTG
CTTCGGGAGAAGTTTGCTCACCTTAACTTGACATTTTCAATTGGAGGACA
GATAAGCTTTGATGTGTTTCCCCAGGGATGGGACAAGACATACTGCTTGA
GATACCTTGATGACTTCAATGAAATTCACTTTTTTGGGGACAAAACTTAC
AAGGGAGGGAATGATCATGAAATTTTTGAATCCGAGCGAACAGTGGGTCA
CACAGTTACCAGCCCTGAGGATACTATGGAAAAGTGTAAGGCTCTCTTCC
TGGCTAAACCGTGAATTTGGGAGAAGTATAGTAAGTCTACAACACGAGAG
AATAATTCGTATTTCTATGCGAGCAATTCATGTATTTTATTTGATCATTG
AGAAGGCATTGCCANTGGGATCGTTGTTTTGATG
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