EY744464

Overview
NameEY744464
Unique NameEY744464
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1062
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY744464 vs. ExPASy Swiss-Prot
Match: E13B_WHEAT (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum GN=GLC1 PE=2 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 1.047e-33
Identity = 66/117 (56.41%), Postives = 84/117 (71.79%), Query Frame = 1
Query:   34 PSTGRFRKGYDRLIFARILEFHRQTKSPFMVNPYPYFGFKPQTLNYALFKPNAGVFDPATGKNYTNMFDAQLDAVYSAMKKVGYEDVDIGVGETGRQKAAQGNHAESNLAHALP*NE 384
            PS   FR GY+  +F  +L+FHR T SPFMVNPYPYF ++P+TLNYALF+PN+G++DPAT  NYT+M DAQ+DA+Y+AMKK+GY DVDI VGE G    A+       +  A   NE
Sbjct:  173 PSNASFRAGYNTKLFPAMLQFHRDTGSPFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAMKKLGYGDVDIAVGEAGWPTQAEPGQIGVGVQEARDFNE 289          
BLAST of EY744464 vs. ExPASy Swiss-Prot
Match: E137_ARATH (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 112.464 bits (280), Expect = 4.409e-24
Identity = 57/123 (46.34%), Postives = 80/123 (65.04%), Query Frame = 1
Query:   13 ILSTSEPPSTGRFRKGYDRLIFARILEFHRQTKSPFMVNPYPYFGF----KPQTLNYALFKPNAGVFDPATGKNYTNMFDAQLDAVYSAMKKVGYEDVDIGVGETGRQKAAQGNHAESNLAHA 369
            +L +S+PPS+G F  GY   +   IL+F   T SPF +NPYP+F +    +P+TL + LF+PNAG  D  TG  YTNMFDAQ+DAV+SA+K +G+E V+I V ETG       N   +++ +A
Sbjct:  162 VLGSSDPPSSGSFAAGYQTGLKG-ILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNA 283          
BLAST of EY744464 vs. ExPASy Swiss-Prot
Match: E1314_ARATH (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana GN=At2g27500/F10A12.18 PE=1 SV=2)

HSP 1 Score: 102.064 bits (253), Expect = 5.958e-21
Identity = 48/106 (45.28%), Postives = 73/106 (68.87%), Query Frame = 1
Query:   13 ILSTSEPPSTGRFRKGYDRLIFARILEFHRQTKSPFMVNPYPYFGFKPQT----LNYALFKPNAGVFDPATGKNYTNMFDAQLDAVYSAMKKVGYEDVDIGVGETG 318
            ILSTS PPS+G F++ + + +   +L+FH Q +SPF++N YP+F +K       L Y LF+PN G+ DP T  +Y NM  AQ+DA+YSA+K +G+ D+++ + ETG
Sbjct:  165 ILSTSYPPSSGSFKEEFIQYL-QPLLDFHSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269          
BLAST of EY744464 vs. ExPASy Swiss-Prot
Match: E1311_ARATH (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana GN=At1g32860 PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 5.044e-20
Identity = 51/107 (47.66%), Postives = 70/107 (65.42%), Query Frame = 1
Query:   13 ILSTSEPPSTGRFRKGY-DRLIFARILEFHRQTKSPFMVNPYPYFGFKPQT----LNYALFKPNAGVFDPATGKNYTNMFDAQLDAVYSAMKKVGYEDVDIGVGETG 318
            IL +S PPS G F+    D L    ILEFHR+T SPF++N YP+F +K       L++ LF+PN G+ DPATG +Y NM  AQ+DAVYSA+   G++ + + + ETG
Sbjct:  164 ILKSSFPPSAGEFQPDLLDSL--TPILEFHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSALAAAGFKSLRVEISETG 268          
BLAST of EY744464 vs. ExPASy Swiss-Prot
Match: E1312_ARATH (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana GN=At4g29360 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 6.817e-17
Identity = 47/106 (44.34%), Postives = 69/106 (65.09%), Query Frame = 1
Query:   13 ILSTSEPPSTGRFRKGYDRLIFARILEFHRQTKSPFMVNPYPYFGFKPQT----LNYALFKPNAGVFDPATGKNYTNMFDAQLDAVYSAMKKVGYEDVDIGVGETG 318
            ILS S PPS+  F K +   +   +LEF  + +SPFM++ YPY+ ++  T    L YALF+ ++ V DPATG  Y+NMFDAQLDA+Y A+  + ++ V + V E+G
Sbjct:  162 ILSRSFPPSSASFSKKHSAFL-KPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVTESG 266          
BLAST of EY744464 vs. ExPASy Swiss-Prot
Match: E1313_ARATH (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana GN=At5g56590 PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.983e-16
Identity = 50/126 (39.68%), Postives = 73/126 (57.94%), Query Frame = 1
Query:   13 ILSTSEPPSTGRFRKGYDRLIFARILEFHRQTKSPFMVNPYPYFGFKPQ----TLNYALFKPNAGVFDPATGKNYTNMFDAQLDAVYSAMKKVGYEDVDIGVGETG--------RQKAAQGNHAES 354
            ILS S PPS G F   Y   +   +LEF  + KSPFM++ YPY+ ++      +L+Y LF+ ++ V DP TG  Y NMFDAQ+DA+Y A+  + +  + I V ETG         + AA  ++AE+
Sbjct:  162 ILSRSFPPSAGAFNSSYAYFL-RPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAET 286          
BLAST of EY744464 vs. ExPASy Swiss-Prot
Match: E1310_ARATH (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana GN=At5g42100 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 1.421e-14
Identity = 49/109 (44.95%), Postives = 65/109 (59.63%), Query Frame = 1
Query:   13 ILSTSEPPSTGRFRKGYDRL-IFARILEFHRQTKSPFMVNPYPYFGF----KPQTLNYALFKPNAGVFDPATGKNYTNMFDAQLDAVYSAMKKVG--YEDVDIGVGETG 318
            IL  S PPS   FR+  D L     IL+FH +T SP ++N YP+F +    K  +L++ LF+PN G  DP +  +Y NM  AQ+DAVY A+  VG  Y+ V I V ETG
Sbjct:  162 ILDVSYPPSATSFRR--DLLGSLTPILDFHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVYHALDAVGISYKKVPIVVSETG 268          
BLAST of EY744464 vs. ExPASy Swiss-Prot
Match: E13E_HORVU (Glucan endo-1,3-beta-glucosidase GV OS=Hordeum vulgare PE=2 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 1.572e-13
Identity = 41/110 (37.27%), Postives = 63/110 (57.27%), Query Frame = 1
Query:    4 RVRILSTSEPPSTGRFRKGYDRLIFARILEFHRQTKSPFMVNPYPYFGFKPQT-----LNYALFKPNAGVFDPATGKNYTNMFDAQLDAVYSAMKKVGYEDVDIGVGETG 318
            R  +++ S PPS+G FR     +  AR L+    T +PF+ N YPYF ++        LNYA  +P   V D   G  YT++FDA +D++Y+A++K G  +V + V E+G
Sbjct:  135 RFDVITNSFPPSSGVFRDPSGLVPIARFLD---STGAPFLANVYPYFAYRDDRGQNIRLNYATLQPGTTVRDNGNGLTYTSLFDAMVDSIYAALEKAGTPNVRVVVSESG 241          
BLAST of EY744464 vs. ExPASy Swiss-Prot
Match: E134_ARATH (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana GN=At3g13560 PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 4.573e-13
Identity = 42/120 (35.00%), Postives = 69/120 (57.50%), Query Frame = 1
Query:   31 PPSTGRFRKGYDRLIFARILEFHRQTKSPFMVNPYPYFGFKPQT----LNYALFK---PNAGVFDPATGKNYTNMFDAQLDAVYSAMKKVGYEDVDIGVGETGRQKAAQGNHAESNLAHA 369
            PPST  F   ++  ++ ++L+F + T S FM+N YPY+G+        L+YALFK   P   + DP T  +Y +MFDA +DA Y +M+ + +  + + V ETG   +   + A + +A+A
Sbjct:  167 PPSTSTFSPSWNTTVY-QLLQFLKNTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAYYSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANA 285          
BLAST of EY744464 vs. ExPASy Swiss-Prot
Match: E13B_HORVU (Glucan endo-1,3-beta-glucosidase GII OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.019e-12
Identity = 42/105 (40.00%), Postives = 61/105 (58.10%), Query Frame = 1
Query:   16 LSTSEPPSTGRFRKGYDRLIFARILEFHRQTKSPFMVNPYPYFGFKPQ----TLNYALFKPNAGVFDPATGKNYTNMFDAQLDAVYSAMKKVGYEDVDIGVGETG 318
            ++ S PPS G F+  Y   + AR+L     T +P + N YPYF ++      +LNYA F+P   V D   G  YT++FDA +DAVY+A++K G   V + V E+G
Sbjct:  161 VANSFPPSAGVFKNAYMTDV-ARLLA---STGAPLLANVYPYFAYRDNPGSISLNYATFQPGTTVRDQNNGLTYTSLFDAMVDAVYAALEKAGAPAVKVVVSESG 261          
The following BLAST results are available for this feature:
BLAST of EY744464 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
E13B_WHEAT1.047e-3356.41Glucan endo-1,3-beta-glucosidase OS=Triticum aesti... [more]
E137_ARATH4.409e-2446.34Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis ... [more]
E1314_ARATH5.958e-2145.28Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis... [more]
E1311_ARATH5.044e-2047.66Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis... [more]
E1312_ARATH6.817e-1744.34Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis... [more]
E1313_ARATH1.983e-1639.68Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis... [more]
E1310_ARATH1.421e-1444.95Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis... [more]
E13E_HORVU1.572e-1337.27Glucan endo-1,3-beta-glucosidase GV OS=Hordeum vul... [more]
E134_ARATH4.573e-1335.00Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis ... [more]
E13B_HORVU1.019e-1240.00Glucan endo-1,3-beta-glucosidase GII OS=Hordeum vu... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-008-H06-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY744464 ID=EY744464; Name=EY744464; organism=Citrus sinensis; type=EST; length=1062bp
CCACGCGTCCGGATTTTATCAACCTCTGAGCCGCCGAGTACTGGAAGGTT
CCGTAAAGGTTACGATAGGCTTATCTTTGCTAGGATTCTCGAGTTCCACA
GACAGACTAAATCCCCGTTTATGGTTAACCCCTACCCGTATTTTGGGTTC
AAGCCTCAGACGCTCAACTACGCATTGTTTAAACCAAACGCCGGCGTTTT
TGATCCTGCCACGGGGAAGAATTACACCAACATGTTTGACGCCCAGCTTG
ATGCGGTTTACTCGGCTATGAAGAAAGTTGGGTACGAAGACGTGGACATT
GGGGTTGGGGAAACTGGGAGGCAGAAGGCGGCGCAGGGCAACCACGCGGA
GTCCAACTTGGCACACGCCCTCCCCTGAAACGAACAACCCAAGCGGAGCT
CGAGCCTCGGAAGGCGGAGGCCCCTGCGACCCCGCAGACGGAGGCCGGAC
GGGATGGGCGGGCGCGCGCAACGGCGAACACCGCGCGTGGCGTCACGCCC
GACGCCGACGCGCCCCGAGCCCCGCGGGCCAGACAGGACGCCGAGACGCC
GGAGAGGAGGAGCAGCCGGGGGCCCGGACGCGGGCGGAGCGCCGACGGGG
CGCCAGCCGGCCGGCCTCGGGGCAGAGGCCGCACGTAGGGAGACAGGTCG
GCAGCCGCAGGCGCGCGCCGCTCGAGGGCCGGGTGGCCCGGACACAGCGA
CCCGGCGCGCGGCGGCCCGGAATAGCTGCGGCGCATTCCAACCGCGGCGG
CAGCGGCCACCCAGCCGAGCACGCCCCCCCGCGGCCACCCGGCCAGCGCC
CAGCAACCCCCGCGCCCAGAGATGCCGCAACCAAACAGTACACCGCGGCC
AGCACCCACGGGGTCCCTCACCCGCGGGACACGGACGCAGAACACAGCCT
CGCGGCCCCGCCGGCGCACCGGCGACGGCCGGGGCCGGGAGCGCGCCGCA
GGACGACACTGAGGGCACAACCGGCAGAGCGACCGCGCGGGGCAAGCACC
CCGCGTCGCCAAGGCGTAGCGGCTCACCACAAGGACGCCGCGGACGCGGA
GCGGCGCCCGCC
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