EY744414

Overview
NameEY744414
Unique NameEY744414
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length961
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY744414 vs. ExPASy Swiss-Prot
Match: TCMO_POPTM (Trans-cinnamate 4-monooxygenase OS=Populus tremuloides GN=CYP73A13 PE=2 SV=1)

HSP 1 Score: 322.398 bits (825), Expect = 2.411e-87
Identity = 157/199 (78.89%), Postives = 166/199 (83.42%), Query Frame = 3
Query:   15 INVAAIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKXXXXXXXXXXXXXSKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAKKGGQFILHILKHSTIGGK 611
            INVAAIETTLWSIEWGIAELVNHPEIQKKLR+ELDT+LGPGHQITEPDT+KLPYL AV+KETLRLRMAIPLLVPHMNLHDAKLGG+D+PAESKILVNAWWLANNPA WK              +KVEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQSKIDT++KGGQF LHILKHSTI  K
Sbjct:  303 INVAAIETTLWSIEWGIAELVNHPEIQKKLRHELDTLLGPGHQITEPDTYKLPYLNAVVKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKNPEEFRPERFLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKIDTSEKGGQFSLHILKHSTIVAK 501          
BLAST of EY744414 vs. ExPASy Swiss-Prot
Match: TCMO_POPKI (Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis GN=CYP73A16 PE=2 SV=1)

HSP 1 Score: 322.398 bits (825), Expect = 2.411e-87
Identity = 157/199 (78.89%), Postives = 166/199 (83.42%), Query Frame = 3
Query:   15 INVAAIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKXXXXXXXXXXXXXSKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAKKGGQFILHILKHSTIGGK 611
            INVAAIETTLWSIEWGIAELVNHPEIQKKLR+ELDT+LGPGHQITEPDT+KLPYL AV+KETLRLRMAIPLLVPHMNLHDAKLGG+D+PAESKILVNAWWLANNPA WK              +KVEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQSKIDT++KGGQF LHILKHSTI  K
Sbjct:  303 INVAAIETTLWSIEWGIAELVNHPEIQKKLRHELDTLLGPGHQITEPDTYKLPYLNAVVKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKNPEEFRPERFLEEGAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKIDTSEKGGQFSLHILKHSTIVAK 501          
BLAST of EY744414 vs. ExPASy Swiss-Prot
Match: TCMO_GLYEC (Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata GN=CYP73A14 PE=2 SV=1)

HSP 1 Score: 319.701 bits (818), Expect = 1.563e-86
Identity = 156/199 (78.39%), Postives = 164/199 (82.41%), Query Frame = 3
Query:   15 INVAAIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKXXXXXXXXXXXXXSKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAKKGGQFILHILKHSTIGGK 611
            INVAAIETTLWSIEWGIAELVNHPEIQKK+R+E+D VLGPGHQ+TEPD  KLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYD+PAESKILVNAWWLANNPA WK+             S VEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQSK+DTA+KGGQF LHILKHSTI  K
Sbjct:  303 INVAAIETTLWSIEWGIAELVNHPEIQKKVRDEIDRVLGPGHQVTEPDMQKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKRPEEFRPERFLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLDTAEKGGQFSLHILKHSTIVAK 501          
BLAST of EY744414 vs. ExPASy Swiss-Prot
Match: TCMO_SOYBN (Trans-cinnamate 4-monooxygenase OS=Glycine max GN=CYP73A11 PE=2 SV=1)

HSP 1 Score: 314.694 bits (805), Expect = 5.028e-85
Identity = 154/199 (77.39%), Postives = 163/199 (81.91%), Query Frame = 3
Query:   15 INVAAIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKXXXXXXXXXXXXXSKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAKKGGQFILHILKHSTIGGK 611
            INVAAIETTLWSIEWGIAELVNHPEIQ+KLR+E+D VLG GHQ+TEPD  KLPYLQAV+KETLRLRMAIPLLVPHMNLHDAKLGGYD+PAESKILVNAWWLANNPA WKK             S VEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQS+IDT++KGGQF LHILKHSTI  K
Sbjct:  304 INVAAIETTLWSIEWGIAELVNHPEIQQKLRDEIDRVLGAGHQVTEPDIQKLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPERFFEEESLVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSQIDTSEKGGQFSLHILKHSTIVAK 502          
BLAST of EY744414 vs. ExPASy Swiss-Prot
Match: TCMO_CATRO (Trans-cinnamate 4-monooxygenase OS=Catharanthus roseus GN=CYP73A4 PE=2 SV=1)

HSP 1 Score: 313.923 bits (803), Expect = 8.576e-85
Identity = 155/196 (79.08%), Postives = 164/196 (83.67%), Query Frame = 3
Query:   15 INVAAIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKXXXXXXXXXXXXXSKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAKKGGQFILHILKHSTI 602
            INVAAIETTLWSIEWGIAELVNHPEIQKKLR+EL+TVLGPG QITEPDT+KLPYLQAVIKETLRLRMAIPL +PHMNLHDAKLGGYD+PAESKILVNAW+LANNP  WKK             SKVEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPG+SKIDT++KGGQF LHILKHSTI
Sbjct:  303 INVAAIETTLWSIEWGIAELVNHPEIQKKLRDELETVLGPGVQITEPDTYKLPYLQAVIKETLRLRMAIPLFLPHMNLHDAKLGGYDIPAESKILVNAWFLANNPEHWKKPEEFRPERFLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGKSKIDTSEKGGQFSLHILKHSTI 498          
BLAST of EY744414 vs. ExPASy Swiss-Prot
Match: TCMO_PHAAU (Trans-cinnamate 4-monooxygenase OS=Phaseolus aureus GN=CYP73A2 PE=1 SV=1)

HSP 1 Score: 313.153 bits (801), Expect = 1.463e-84
Identity = 152/199 (76.38%), Postives = 163/199 (81.91%), Query Frame = 3
Query:   15 INVAAIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKXXXXXXXXXXXXXSKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAKKGGQFILHILKHSTIGGK 611
            INVAAIETTLWSIEWGIAELVNHPEIQ+K+R+E+D VLG GHQ+TEPD  KLPYLQAV+KETLRLRMAIPLLVPHMNLHDAKLGGYD+PAESKILVNAWWLANNPA WKK             S VEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQS+IDT++KGGQF LHILKHST+  K
Sbjct:  303 INVAAIETTLWSIEWGIAELVNHPEIQQKVRDEIDRVLGVGHQVTEPDIQKLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPERFFEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSQIDTSEKGGQFSLHILKHSTVVAK 501          
BLAST of EY744414 vs. ExPASy Swiss-Prot
Match: TCMO_PEA (Trans-cinnamate 4-monooxygenase OS=Pisum sativum GN=CYP73A9 PE=2 SV=2)

HSP 1 Score: 312.768 bits (800), Expect = 1.910e-84
Identity = 154/199 (77.39%), Postives = 161/199 (80.90%), Query Frame = 3
Query:   15 INVAAIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKXXXXXXXXXXXXXSKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAKKGGQFILHILKHSTIGGK 611
            INVAAIETTLWSIEWGIAELVNH EIQ KLR E+D VLGPGHQ+TEPD  KLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGG+D+PAESKILVNAWWLANNPA WKK             + VEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQSKIDT++KGGQF LHILKHSTI  K
Sbjct:  303 INVAAIETTLWSIEWGIAELVNHQEIQNKLREEMDKVLGPGHQVTEPDLEKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPALWKKPEEFRPERFLEEEAHVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKIDTSEKGGQFSLHILKHSTIVAK 501          
BLAST of EY744414 vs. ExPASy Swiss-Prot
Match: TCMO_HELTU (Trans-cinnamate 4-monooxygenase OS=Helianthus tuberosus GN=CYP73A1 PE=1 SV=1)

HSP 1 Score: 312.383 bits (799), Expect = 2.495e-84
Identity = 155/199 (77.89%), Postives = 161/199 (80.90%), Query Frame = 3
Query:   15 INVAAIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKXXXXXXXXXXXXXSKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAKKGGQFILHILKHSTIGGK 611
            INVAAIETTLWSIEWGIAELVNHPEIQ KLR+ELDT LGPG QITEPD   LPYLQAV+KETLRLRMAIPLLVPHMNLHDAKLGG+D+PAESKILVNAWWLANNP QWKK             +KVEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQSKIDT +KGGQF LHILKHSTI  K
Sbjct:  303 INVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPDQWKKPEEFRPERFLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKIDTDEKGGQFSLHILKHSTIVAK 501          
BLAST of EY744414 vs. ExPASy Swiss-Prot
Match: TCMO_CICAR (Trans-cinnamate 4-monooxygenase OS=Cicer arietinum GN=CYP73A19 PE=2 SV=2)

HSP 1 Score: 311.997 bits (798), Expect = 3.259e-84
Identity = 153/196 (78.06%), Postives = 160/196 (81.63%), Query Frame = 3
Query:   15 INVAAIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKXXXXXXXXXXXXXSKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAKKGGQFILHILKHSTI 602
            INVAAIETTLWSIEWGIAELVNH +IQ K+R E+D VLGPGHQ+TEPD  KLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKL G+D+PAESKILVNAWWLANNPAQWKK             S VEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQSKIDTA+KGGQF LHILKHSTI
Sbjct:  302 INVAAIETTLWSIEWGIAELVNHQKIQNKVREEIDRVLGPGHQVTEPDLQKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLSGFDIPAESKILVNAWWLANNPAQWKKPEEFRPERFLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKIDTAEKGGQFSLHILKHSTI 497          
BLAST of EY744414 vs. ExPASy Swiss-Prot
Match: TCMO_ZINEL (Trans-cinnamate 4-monooxygenase OS=Zinnia elegans GN=CYP73A12 PE=2 SV=1)

HSP 1 Score: 311.227 bits (796), Expect = 5.559e-84
Identity = 154/199 (77.39%), Postives = 160/199 (80.40%), Query Frame = 3
Query:   15 INVAAIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDVPAESKILVNAWWLANNPAQWKKXXXXXXXXXXXXXSKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKIDTAKKGGQFILHILKHSTIGGK 611
            INVAAIETTLWSIEW IAELVNHPEIQ KLR+EL + LGPG Q+TEPD HKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYD+PAESKILVNAWWLANNP QWKK             SKVEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQ K+DT +KGGQF LHILKHSTI  K
Sbjct:  303 INVAAIETTLWSIEWAIAELVNHPEIQAKLRHELVSQLGPGVQVTEPDLHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPDQWKKPEEFRPERFLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQDKVDTTEKGGQFSLHILKHSTIVAK 501          
The following BLAST results are available for this feature:
BLAST of EY744414 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 290
Match NameE-valueIdentityDescription
TCMO_POPTM2.411e-8778.89Trans-cinnamate 4-monooxygenase OS=Populus tremulo... [more]
TCMO_POPKI2.411e-8778.89Trans-cinnamate 4-monooxygenase OS=Populus kitakam... [more]
TCMO_GLYEC1.563e-8678.39Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza ech... [more]
TCMO_SOYBN5.028e-8577.39Trans-cinnamate 4-monooxygenase OS=Glycine max GN=... [more]
TCMO_CATRO8.576e-8579.08Trans-cinnamate 4-monooxygenase OS=Catharanthus ro... [more]
TCMO_PHAAU1.463e-8476.38Trans-cinnamate 4-monooxygenase OS=Phaseolus aureu... [more]
TCMO_PEA1.910e-8477.39Trans-cinnamate 4-monooxygenase OS=Pisum sativum G... [more]
TCMO_HELTU2.495e-8477.89Trans-cinnamate 4-monooxygenase OS=Helianthus tube... [more]
TCMO_CICAR3.259e-8478.06Trans-cinnamate 4-monooxygenase OS=Cicer arietinum... [more]
TCMO_ZINEL5.559e-8477.39Trans-cinnamate 4-monooxygenase OS=Zinnia elegans ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-008-D03-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY744414 ID=EY744414; Name=EY744414; organism=Citrus sinensis; type=EST; length=961bp
TACATCGTTCAGAAATCAATGTTGCCGCGATCGAGACAACTCTTTGGTCG
ATCGAGTGGGGAATTGCAGAGCTAGTGAATCATCCTGAAATCCAGAAGAA
GCTCCGCAATGAACTTGACACCGTGCTTGGTCCTGGTCATCAAATCACTG
AGCCTGACACCCACAAACTACCCTACCTTCAGGCCGTGATCAAGGAAACT
CTTCGCCTTAGGATGGCAATTCCTCTTCTTGTGCCGCACATGAACCTTCA
CGACGCCAAGCTTGGAGGCTACGACGTCCCTGCTGAGAGCAAAATCCTAG
TGAACGCGTGGTGGCTCGCCAACAATCCCGCCCAATGGAAGAAACCTGAA
GAATTCAGGCCTGAGAGGTTTTTGGAGGAGGAGTCCAAGGTTGAGGCTAA
TGGAAATGACTTCCGTTACCTTCCTTTCGGTGTTGGGAGAAGAAGCTGCC
CTGGAATTATCCTTGCTTTGCCAATTCTTGGTATCACTATTGGGCGTTTG
GTACAGAACTTTGAGCTCTTGCCTCCTCCAGGTCAATCTAAGATTGACAC
TGCAAAGAAAGGTGGGCAGTTCATTTTGCACATTTTGAAGCACTCGACCA
TTGGTGGCAAAGCCTAAAACATTCTAATTTCCACATTTCCATTTTGGTTG
TAATGGTAAAGTCTGGTTTCAAAGGGTTTGAATGGTTTCTTCAATGGCCG
GATCCAAATCCAGGGGCCAGTTGGGCTAATTATTAAACTACTTGGGGTAA
ATTCTTGGAAACTATAAACCTTCGCAATCTTCCCTTTGGGGAAGAGCCAA
CAGAAAAGAGCCAACCCACAGCCAGACAAGAAGCACCAGCAACAACATCA
CCCCGACAAAAGCGGGGGGGGCCGCATCTCCAAAAAAACAAAAAATCTAA
AACAATCACTAGCTGGTAGGAAACGCCCTAAACTTTCTCTCTCTAAGGGA
GTCACCCCTTC
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