EY660155

Overview
NameEY660155
Unique NameEY660155
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length919
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY660155 vs. ExPASy Swiss-Prot
Match: MTAD_METS3 (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) GN=mtaD PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 5.458e-33
Identity = 80/223 (35.87%), Postives = 123/223 (55.16%), Query Frame = 2
Query:   20 SSSTMILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELIHSGVTCFAEAGGQHVSEMAKAVELLGLRACLVQSTMDCGEGLPASWAVRTTDDCIQSQKELYAKHHHAADGRIRIWFGIRQIMNATDRLLLETRDMAREFKQEI 688
            + +T+++ +A+I+       +   G + +  D+I  IG   D         D++ID + +ILLPGFVNTH H S  L +G+ADD+ L +WL+D IWP E+N+T E  YI  LL  IELI SG T F++    ++ ++AKAVE  G+RA L    +D G+           +  I+    L+ K +  ADGRI+++FG      A+  LL + R +A E+   I
Sbjct:    2 TDNTILIKDALILNPLDFKEI--KGSLLIKNDKIAEIGTDID-----ESNVDKVIDAKGKILLPGFVNTHTHLSMTLFRGLADDLSLDSWLNDNIWPMEANLTSEYCYIGALLGAIELIKSGTTTFSDM-YFYMEDVAKAVEESGIRAVLSYGMIDFGDD-------EKREHEIKENIALFEKCNGMADGRIKVFFGPHSPYTASKDLLEDVRWLANEYNTGI 209          

HSP 2 Score: 32.3426 bits (72), Expect = 5.458e-33
Identity = 17/50 (34.00%), Postives = 29/50 (58.00%), Query Frame = 1
Query:  679 TGNHMHVAEIPYEKSVVMDTXKSDHGTGTFLDKIEFLQNNLLSAHTVWVT 828
            TG H+HV+E   +K +       D     +LD I FL  ++++AH+VW++
Sbjct:  207 TGIHIHVSET--QKEINDSLEAHDLRPFEYLDSIGFLGPDVVAAHSVWLS 254          
BLAST of EY660155 vs. ExPASy Swiss-Prot
Match: MTAD_METBF (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Methanosarcina barkeri (strain Fusaro / DSM 804) GN=mtaD PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 6.904e-28
Identity = 79/237 (33.33%), Postives = 116/237 (48.95%), Query Frame = 2
Query:   32 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELIHSGVTCFAEAGGQHVSEMAKAVELLGLRACLVQSTMDCGEGLPASWAVRTTDDCIQSQKELYAKHHHAADGRIRIWFGIRQIMNATDRLLLETRDMAR-----------EFKQEITCMLQRY 709
            +I+ NA ++TMD +    +NG V +   +I  IG+        S+ AD +ID +  +++PG VNTH H +  L +G ADD+ L  WL   IWP E+ +T ED Y  +LL  +E+I SG T FA+    ++ E AKAVE  GLRA L         GL   W        ++  K        AADGRI+  +G       ++  L + R+ A            E + E+  M +RY
Sbjct:    4 IIVKNAYVMTMDPDEGDLKNGTVVIEDGKITEIGEKT------SESADTVIDAKHSVVMPGLVNTHTHAAMTLFRGYADDLQLADWLEGHIWPAEAKLTAEDVYKGSLLACLEMIRSGTTSFADM-YFYMDETAKAVEASGLRASLC-------HGLIELWNEEKGATDLKEGKRFVRAWQGAADGRIKTMYGPHAPNTCSEEFLAKVREEANRDGAGIHIHLLETEAELLAMKERY 226          
BLAST of EY660155 vs. ExPASy Swiss-Prot
Match: MTAD_METMA (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Methanosarcina mazei GN=mtaD PE=3 SV=2)

HSP 1 Score: 122.094 bits (305), Expect = 4.475e-27
Identity = 77/237 (32.49%), Postives = 115/237 (48.52%), Query Frame = 2
Query:   32 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELIHSGVTCFAEAGGQHVSEMAKAVELLGLRACLVQSTMDCGEGLPASWAVRTTDDCIQSQKELYAKHHHAADGRIRIWFGIRQIMNATDRLLLETRDMAR-----------EFKQEITCMLQRY 709
            +I+ NA ++TMD ++   + G V +   +I  IG          + AD +ID +  +++PG VNTH H +  L +G ADD+ L  WL   IWP E+ +T ED Y  +LL  +E+I SG T FA+     + E AKAVE  GLRA L         GL   W     ++ ++  K        AA GRI+  +G       +D  L + ++ AR           E + E+  M +RY
Sbjct:    4 IIIKNAYVLTMDPDAGDIKKGTVVIEDGKITEIGVKT------KESADTVIDAKGSVVMPGLVNTHTHAAMTLFRGYADDLQLAEWLEKHIWPAEAQLTAEDVYRGSLLACLEMIRSGTTSFADM-YFFMDETAKAVEASGLRASL-------SHGLIELWNEEKGENDLKEGKRFVRAWQGAAKGRIKTMYGPHAPNTCSDEFLAKVKEAARQDGAGLHIHVLETEAELLAMKERY 226          
BLAST of EY660155 vs. ExPASy Swiss-Prot
Match: MTAD_METAC (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Methanosarcina acetivorans GN=mtaD PE=3 SV=2)

HSP 1 Score: 120.939 bits (302), Expect = 9.969e-27
Identity = 75/237 (31.65%), Postives = 116/237 (48.95%), Query Frame = 2
Query:   32 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELIHSGVTCFAEAGGQHVSEMAKAVELLGLRACLVQSTMDCGEGLPASWAVRTTDDCIQSQKELYAKHHHAADGRIRIWFGIRQIMNATDRLLLETRDMAR-----------EFKQEITCMLQRY 709
            +I+ NA ++TMD ++   +NG V +   +I  IG++        + AD +ID +  +++PG  NTH H +  L +G ADD+ L  WL   IWP E+ +  ED Y  +LL  +E+I SG T FA+    ++ E AKAVE  GLRA L         GL   W     +  ++  K        AADGRI+  +G       ++  L + ++ A            E + E+  M +RY
Sbjct:    4 IIIKNAYVLTMDPDAGDLKNGTVVIEDGKITEIGENT------KENADTVIDAKGSVVMPGLANTHTHAAMTLFRGYADDLQLAEWLEKHIWPAEAQLKAEDVYKGSLLACLEMIKSGTTSFADM-YFYMDETAKAVEASGLRASL-------SHGLIELWNEEKGEADLKEGKRFVRAWQGAADGRIKTMYGPHAPNTCSEEFLTKVKEEAHRDGAGLHIHVLETEAELNAMKERY 226          
BLAST of EY660155 vs. ExPASy Swiss-Prot
Match: MTAD_DESRM (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Desulfotomaculum reducens (strain MI-1) GN=mtaD PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 4.188e-25
Identity = 62/190 (32.63%), Postives = 98/190 (51.58%), Query Frame = 2
Query:   32 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELIHSGVTCFAEAGGQHVSEMAKAVELLGLRACLVQSTMDCGEGLPASWAVRTTDDCIQSQKELYAKHHHAADGRIRI 601
            +++  A I+TM+    +   G + +    I  +G        F    D++I+   Q+ +PGF+N H H +  L +G ADD+ LMTWL ++IWP+E  MT ED Y  T+L  +E+I SG TCF +     + ++A+AVE  G+RA L +  +             T D  +   +EL    +  ADGRI +
Sbjct:    4 LLIRGATILTMEGPEAIIETGELLIEDGWITHVGLPGSASGSFDM--DEVIEADGQVAMPGFINCHTHAAMTLLRGYADDLPLMTWLSEKIWPFEGRMTNEDIYWGTMLACLEMIKSGTTCFGDM-YDCMHDVARAVEKTGMRAMLSRGMIGIAP---------TADKALIEAEELARNWNGKADGRITV 181          
BLAST of EY660155 vs. ExPASy Swiss-Prot
Match: MTAD_PELTS (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) GN=mtaD PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 3.546e-24
Identity = 67/214 (31.31%), Postives = 108/214 (50.47%), Query Frame = 2
Query:   26 STMILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELIHSGVTCFAEAGGQHVSEMAKAVELLGLRACLVQSTMDCGEGLPASWAVRTTDDCIQSQKELYAKHHHAADGRIRIWFGIRQIMNATDRLLLETRDMA 667
            + +++  A ++TM+    V+R+G + +  + I ++G    + + F     + ID    + +PGFVN H H +  L +  ADD+ LM WL ++IWP E  +  ED Y  T+LC +E+I SG T FA+     +  +A AVE  G+RACL +  +  G G     A +  D+ +   +E     +  ADGRI   FG           L +  D+A
Sbjct:    2 ANILIQGATVLTMEGPDGVYRDGEIAIAGNSILSVGPRGSVPEGFRP--GRSIDGTGMVAMPGFVNCHTHAAMTLLRSYADDMPLMKWLSEKIWPVEERLQPEDIYWGTMLCCLEMIKSGTTTFADMYFS-MERVAAAVEESGMRACLSRGMIGVGSG-----ARKAIDESLSFVRE----WNGGADGRITAMFGPHAPYTCPPEYLKKVVDLA 203          
BLAST of EY660155 vs. ExPASy Swiss-Prot
Match: MTAD_METBU (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Methanococcoides burtonii (strain DSM 6242) GN=mtaD PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 4.716e-24
Identity = 66/212 (31.13%), Postives = 105/212 (49.53%), Query Frame = 2
Query:   32 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELIHSGVTCFAEAGGQHVSEMAKAVELLGLRACLVQSTMDCGEGLPASWAVRTTDDCIQSQKELYAKHHHAADGRIRIWFGIRQIMNATDRLLLETRDMA 667
            +I+ N  ++TMD E     NG V V++      G+  ++ +  S  A+ +ID Q  +++PGFVNTH H    L +G ADD+ L  WL + IWP E+ +T  D    T L  +E+I SG   FA+     + E+ KAVE  GLRA L         G+   W      + ++  +E   + +  A+GRI + +G       +   L + ++ A
Sbjct:    4 IIIKNGYVLTMDPEVDDIPNG-VVVIEG-----GKIVEVAETTSATANTVIDAQGGVVMPGFVNTHTHAGMTLFRGYADDLPLAQWLQEHIWPAEAELTASDVLAGTRLACLEMIKSGTIAFADM-YFFMEEVGKAVEECGLRAAL-------SYGMIELWDDEKGTNELKKGREFVKEWNGKAEGRISVMYGPHAPNTCSKEFLSKVKEQA 201          

HSP 2 Score: 27.7202 bits (60), Expect = 4.716e-24
Identity = 15/49 (30.61%), Postives = 26/49 (53.06%), Query Frame = 1
Query:  688 HMHVAEIPYEKSVVMDTXKSDHG--TGTFLDKIEFLQNNLLSAHTVWVT 828
            H+HV E   E    ++  K  +G  +   LD I+F    +L+AH +W++
Sbjct:  209 HIHVLETEAE----LNQMKEQYGMCSVNMLDTIDFFGPGVLAAHCIWLS 253          
BLAST of EY660155 vs. ExPASy Swiss-Prot
Match: MTAD_SYNWW (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Syntrophomonas wolfei subsp. wolfei (strain Goettingen) GN=mtaD PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 6.048e-24
Identity = 73/237 (30.80%), Postives = 115/237 (48.52%), Query Frame = 2
Query:   32 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQSADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELIHSGVTCFAEAGGQHVSEMAKAVELLGLRACLVQSTMDCGEGLPASWAVRTTDDCIQSQKELYAKHHHAADGRIRIWFG-----------IRQIMNATDRLLLETRDMAREFKQEITCMLQRY 709
            ++L N  I+ +   +     G + +    IK +G       +F    D IID ++Q+LLPGF+N H H +  L +G ADD+ LM WL ++IWP E+ +T ED Y  T+L  +E+I SG T F +     + E+A+AVEL G+RA L +  +  G           ++  I+  +EL  K    A GRI    G           + ++M  +D L         E + E   +L++Y
Sbjct:    3 ILLDNISIIPVSGSNSFIEKGYLLIEDVFIKELGTGKAPEGEF----DHIIDGENQVLLPGFINAHTHAAMTLLRGYADDLPLMEWLENKIWPLEAKLTPEDIYWGTMLAIVEMIKSGTTTFNDM-YFCMDEVARAVELSGMRAVLARGMVGVGP---------ESEQAIEDSRELIGKWQGQAGGRISFRLGPHAPYTCPPAYLERVMQLSDELQAGIHIHVAETRVEYEDILKQY 225          
BLAST of EY660155 vs. ExPASy Swiss-Prot
Match: MTAD_THEPX (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Thermoanaerobacter sp. (strain X514) GN=mtaD PE=3 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 7.899e-24
Identity = 66/220 (30.00%), Postives = 110/220 (50.00%), Query Frame = 2
Query:   32 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQ-SADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELIHSGVTCFAEAGGQHVSEMAKAVELLGLRACLVQSTMDCGEGLPASWAVRTTDDCIQSQKELYAKHHHAADGRIRIWFGIRQIMNATDRLLLETRDMAREFKQEI 688
            +++ N  +++M +E  +  N  +++  D I  IG+ + DI        D++ID   +I  PG +N H H    L +  ADDV L  WL   IWP ES ++ ED Y  +LL  IE+I+SG T F +     + E+AKA E +G+R  L +  ++  +            + ++  ++LY   H+ ADGRI++  G       +   L E  ++A+E    I
Sbjct:    3 LLIKNVALLSMKEEQPLMENTNIYIEGDTITYIGEINPDI------KVDRVIDGTKKIATPGLINAHTHLGMSLLRNYADDVPLFDWLSKHIWPVESKLSAEDVYWGSLLSMIEMIYSGTTTFCDM-YFFMDEVAKATEEVGIRGVLTRGIIEESD-------AEINKEKLRDTRKLYNTWHNKADGRIKVMVGPHAPYTCSSSYLKEVVELAKELNTGI 208          
BLAST of EY660155 vs. ExPASy Swiss-Prot
Match: MTAD_THEP3 (5-methylthioadenosine/S-adenosylhomocysteine deaminase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) GN=mtaD PE=3 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 7.899e-24
Identity = 66/220 (30.00%), Postives = 110/220 (50.00%), Query Frame = 2
Query:   32 MILHNAVIVTMDKESRVFRNGGVFVVQDRIKAIGQ-SADILQQFSQMADQIIDLQSQILLPGFVNTHVHTSQQLAKGIADDVDLMTWLHDRIWPYESNMTEEDSYISTLLCGIELIHSGVTCFAEAGGQHVSEMAKAVELLGLRACLVQSTMDCGEGLPASWAVRTTDDCIQSQKELYAKHHHAADGRIRIWFGIRQIMNATDRLLLETRDMAREFKQEI 688
            +++ N  +++M +E  +  N  +++  D I  IG+ + DI        D++ID   +I  PG +N H H    L +  ADDV L  WL   IWP ES ++ ED Y  +LL  IE+I+SG T F +     + E+AKA E +G+R  L +  ++  +            + ++  ++LY   H+ ADGRI++  G       +   L E  ++A+E    I
Sbjct:    3 LLIKNVALLSMKEEQPLMENTNIYIEGDTITYIGEINPDI------KVDRVIDGTKKIATPGLINAHTHLGMSLLRNYADDVPLFDWLSKHIWPVESKLSAEDVYWGSLLSMIEMIYSGTTTFCDM-YFFMDEVAKATEEVGIRGVLTRGIIEESD-------AEINKEKLRDTRKLYNTWHNKADGRIKVMVGPHAPYTCSSSYLKEVVELAKELNTGI 208          
The following BLAST results are available for this feature:
BLAST of EY660155 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 79
Match NameE-valueIdentityDescription
MTAD_METS35.458e-3335.875-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_METBF6.904e-2833.335-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_METMA4.475e-2732.495-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_METAC9.969e-2731.655-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_DESRM4.188e-2532.635-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_PELTS3.546e-2431.315-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_METBU4.716e-2431.135-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_SYNWW6.048e-2430.805-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_THEPX7.899e-2430.005-methylthioadenosine/S-adenosylhomocysteine deami... [more]
MTAD_THEP37.899e-2430.005-methylthioadenosine/S-adenosylhomocysteine deami... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-019-B06-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY660155 ID=EY660155; Name=EY660155; organism=Citrus sinensis; type=EST; length=919bp
CCACGCGTCCGGCTTAGGCTCAAGCTCAACGATGATACTTCACAACGCGG
TGATTGTTACAATGGACAAAGAGAGCCGAGTATTTCGAAACGGCGGCGTT
TTCGTCGTACAAGACCGAATCAAAGCCATCGGTCAATCTGCCGATATTCT
CCAACAATTCTCTCAAATGGCCGATCAAATCATCGATCTCCAAAGCCAAA
TCTTACTTCCAGGATTTGTTAACACGCATGTGCACACATCTCAACAACTG
GCGAAGGGGATAGCTGATGACGTTGATTTGATGACATGGTTGCATGATCG
AATTTGGCCTTACGAATCTAACATGACTGAGGAGGATTCTTACATCTCCA
CTTTACTGTGCGGAATTGAACTTATTCACTCTGGTGTAACGTGTTTTGCT
GAAGCTGGAGGGCAGCATGTAAGTGAAATGGCTAAAGCGGTTGAGTTACT
GGGTCTACGTGCATGTTTAGTCCAGTCAACCATGGACTGTGGGGAGGGTT
TACCTGCATCTTGGGCTGTTCGCACTACAGATGATTGTATTCAGTCTCAA
AAGGAGCTTTATGCGAAGCACCATCATGCAGCCGATGGGCGCATCAGAAT
ATGGTTTGGAATTAGACAAATCATGAATGCAACTGATCGCTTGCTACTTG
AAACAAGAGACATGGCTAGAGAATTTAAACAGGAAATCACATGCATGTTG
CAGAGATACCCTACGAGAAATCAGTTGTGATGGATACTNCGAAAAGTGAT
CACGGGACTGGTACATTCTTGGACAAGATAGAATTCCTGCAGAACAACTT
GCTTTCGGCTCATACAGTTTGGGTCACCACACTGAGATGGTCTCCTCTCA
AGGCTGGGGGCAAAGATCTCACTGGCCTGCTTCTGCATGGGAATGCTTGG
TTTGCTCCATAAGGAAATG
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