EY649981

Overview
NameEY649981
Unique NameEY649981
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length865
Libraries
Library NameType
Sweet orange leaf, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY649981 vs. ExPASy Swiss-Prot
Match: C76B1_HELTU (7-ethoxycoumarin O-deethylase OS=Helianthus tuberosus GN=CYP76B1 PE=1 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 2.407e-34
Identity = 71/178 (39.89%), Postives = 113/178 (63.48%), Query Frame = 3
Query:   33 LLINCILWLAFTLVWVMALSFVPKRRHKQLAPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGHVTTVWVSSPSMAKAILQNHDLSFCDRMDPETILSLPFPHHEFSLGWLPLSTLWKNLRKISNMHIFTRQKLDAYQELRRTTIKDLLAHVENNCRAGKAINVGQAALNT 566
            L+I   L L++ L+WV+ +      + K L PGP   P+IGNL  LGA PH+SLAKLAKIHGPIMSL+LG +TT+ +SS + A+ +L+  DL+F  R  P+ + +  + H   S+ +L + T W+ LR+I + +IF+   L+A Q LR   +++L+A+      + + +++G+AA  T
Sbjct:    4 LIIVSTLLLSYILIWVLGVG-----KPKNLPPGPTRLPIIGNLHLLGALPHQSLAKLAKIHGPIMSLQLGQITTLVISSATAAEEVLKKQDLAFSTRNVPDAVRA--YNHERHSISFLHVCTEWRTLRRIVSSNIFSNSSLEAKQHLRSKKVEELIAYCRKAALSNENVHIGRAAFRT 174          

HSP 2 Score: 30.4166 bits (67), Expect = 2.407e-34
Identity = 21/72 (29.17%), Postives = 37/72 (51.39%), Query Frame = 1
Query:  589 TIGSIDVANPNE---REFNDTVFWCLGRSMIS*LVRPFYYCQKFDLHVIKRRMTVYSAKCFQVXDRFVDQRL 795
            TI S D+ +P E   +EF + +   +  S  + LV  F   +K D   IKR M  + +K   + D+ +++R+
Sbjct:  182 TIFSKDLTDPYEDSGKEFREVITNIMVDSAKTNLVDVFPVLKKIDPQGIKRGMARHFSKVLGIFDQLIEERM 253          
BLAST of EY649981 vs. ExPASy Swiss-Prot
Match: C76C3_ARATH (Cytochrome P450 76C3 OS=Arabidopsis thaliana GN=CYP76C3 PE=2 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 6.811e-27
Identity = 58/149 (38.93%), Postives = 90/149 (60.40%), Query Frame = 3
Query:  120 LAPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGHVTTVWVSSPSMAKAILQNHDLSFCDRMDPETILSLPFPHHEFSLGWLPLSTLWKNLRKISNMHIFTRQKLDAYQELRRTTIKDLLAHVENNCRAGKAINVGQAALNT 566
            L PGP   P++GN+ +LG  PH+SLA  +K +GPIMSL+LG +T V +SSP  AK  L+ HD     R   + + +  F HH+ S+ W+P S  W+ L+K    ++ + Q LDA Q LR   +++L++ V      G+AI++ +A+  T
Sbjct:   38 LPPGPPILPLVGNIFQLGFNPHRSLAAFSKTYGPIMSLKLGRLTAVVISSPEAAKEALRTHDHVMSARTFNDALRA--FDHHKHSIVWIPPSARWRFLKKTITKYLLSPQNLDAIQSLRMRKVEELVSLVNEFRERGEAIDLARASFVT 184          

HSP 2 Score: 28.8758 bits (63), Expect = 6.811e-27
Identity = 20/70 (28.57%), Postives = 34/70 (48.57%), Query Frame = 1
Query:  598 SIDVA----NPNEREFNDTVFWCLGRSMIS*LVRPFYYCQKFDLHVIKRRMTVYSAKCFQVXDRFVDQRL 795
            S+D+A    N +  EF++TV      + I  +   F Y +  DL   +++  +   K F+V   F+D RL
Sbjct:  195 SVDLATYDSNSSSYEFHNTVVHLTDIAGIPNVGDYFQYMRFLDLQGTRKKAVLCIEKLFRVFQEFIDARL 264          
BLAST of EY649981 vs. ExPASy Swiss-Prot
Match: C76C4_ARATH (Cytochrome P450 76C4 OS=Arabidopsis thaliana GN=CYP76C4 PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 1.036e-22
Identity = 62/178 (34.83%), Postives = 102/178 (57.30%), Query Frame = 3
Query:   36 LINCILWLAFTLVWVMALSFVPKRRHKQLAPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGHVTTVWVSSPSMAKAILQNHDLSFCDRMDPETILSLPFPHHEFSLGWLPLSTL-WKNLRKISNMHIFTRQKLDAYQELRRTTIKDLLAHVENNCRAGKAINVGQAALNT 566
            L+ C +   F L+   A S     R   L PGP   P+IGN+ ++G  PH S A LAKI+GPIMSL+ G + +V ++SP  A+ +L+ HD     R   ++I    F H E S+ WLP S+  W+ LRK+S   +F+ Q+ +A + LR   +++L++ +  +    +A+++ +A+  T
Sbjct:   11 LLFCFISSCF-LISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRC--FGHEEVSVIWLPPSSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTT 185          
BLAST of EY649981 vs. ExPASy Swiss-Prot
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 1.354e-22
Identity = 61/182 (33.52%), Postives = 106/182 (58.24%), Query Frame = 3
Query:   27 MGLLINCILWLAFTLVWVMALSFVPKRRHKQLAPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGHVTTVWVSSPSMAKAILQNHDLSFCDRMDPETILSLPFPHHEFSLGWLPLSTLWKNLRKISNMHIFTRQKLDAYQELRRTTIKDLLAHVENNCRAGKAINVGQAALNTCT 572
            + L++  +++ +F L +++  SF  KR    L PGP+P+P+IGNL+ LG KPH+S A +A+ +GP+M L++G V  V  +S S+A   L+ HD +F  R        + + + +  L + P    W+ LRKI ++H+F+ + LD ++ +R+  +K L   + +     K + +GQ  LN CT
Sbjct:    4 LSLILYTVIF-SFLLQFILR-SFFRKRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAMAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQD--LVFAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALAS--AGQKPVKLGQ-LLNVCT 178          
BLAST of EY649981 vs. ExPASy Swiss-Prot
Match: C76C1_ARATH (Cytochrome P450 76C1 OS=Arabidopsis thaliana GN=CYP76C1 PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.938e-22
Identity = 58/179 (32.40%), Postives = 102/179 (56.98%), Query Frame = 3
Query:   33 LLINCILWLAFTLVWVMALSFVPKRRHKQLAPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGHVTTVWVSSPSMAKAILQNHDLSFCDRMDPETILSLPFPHHEFSLGWLPLSTL-WKNLRKISNMHIFTRQKLDAYQELRRTTIKDLLAHVENNCRAGKAINVGQAALNT 566
            LL+ C +   F L++    S    R    L PGP   P+IGN+  +G  PH+S A+L+K +GP+MSL+LG + TV ++SP  A+ +L+ HD     R     + S+   H + SL WLP S+  W+ LR++S   + + Q+++A + LR   +K+L++ +  +    +++++ + A  T
Sbjct:   10 LLLFCFILSCF-LIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAVRSI--NHQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFIT 185          
BLAST of EY649981 vs. ExPASy Swiss-Prot
Match: C76C2_ARATH (Cytochrome P450 76C2 OS=Arabidopsis thaliana GN=CYP76C2 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 3.334e-21
Identity = 56/171 (32.75%), Postives = 97/171 (56.73%), Query Frame = 3
Query:   57 LAFTLVWVMALSFVPKRRHKQLAPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGHVTTVWVSSPSMAKAILQNHDLSFCDRMDPETILSLPFPHHEFSLGWL-PLSTLWKNLRKISNMHIFTRQKLDAYQELRRTTIKDLLAHVENNCRAGKAINVGQAALNT 566
            L+F +++         R+     PGP   P+IGN+  +G  PH S A L+K +GPIMSL+ G + TV V+SP  A+ +L+ +D     R    +I S+   H + S+ WL P S+ W+ LRK+S   +F+ Q+++A + LR   +K+L++ +  +    +A+++ +A   T
Sbjct:   17 LSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSRTPTNSIRSI--NHDKVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFIT 185          
BLAST of EY649981 vs. ExPASy Swiss-Prot
Match: C71BX_ARATH (Cytochrome P450 71B36 OS=Arabidopsis thaliana GN=CYP71B36 PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 5.687e-21
Identity = 54/173 (31.21%), Postives = 93/173 (53.76%), Query Frame = 3
Query:   33 LLINCILWLAFTLVWVMALSFVPKRRHKQLAPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGHVTTVWVSSPSMAKAILQNHDLSFCDRMDPETILSLPFPHHEFSLGWLPLSTLWKNLRKISNMHIFTRQKLDAYQELRRTTIKDLLAHVENNCRAGKAINVGQ 551
            L ++CIL  AFT           +++H++  P P  +P+IGNL +LG  PH+SL +L+K +G +M L+ G + TV VSS   AK +L+ HDL  C R  P         ++   + + P    WK LR+I    +F+ +++ ++Q ++   +K L+  V  +   G  +N+ +
Sbjct:   10 LFLSCILLAAFT--------HKKRQQHQRKPPSPPGFPIIGNLHQLGELPHQSLWRLSKKYGHVMLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSR--PSLAGPRALSYNYLDIAFSPFDDYWKELRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQGTPVNLSE 172          
BLAST of EY649981 vs. ExPASy Swiss-Prot
Match: C71BC_ARATH (Cytochrome P450 71B12 OS=Arabidopsis thaliana GN=CYP71B12 PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 5.687e-21
Identity = 52/170 (30.59%), Postives = 95/170 (55.88%), Query Frame = 3
Query:   51 LW---LAFTLVWVMALSFVPKRRHKQLAPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGHVTTVWVSSPSMAKAILQNHDLSFCDRMDPETILSLPFPHHEFSLGWLPLSTLWKNLRKISNMHIFTRQKLDAYQELRRTTIKDLLAHVENNCRAGKAINVGQ 551
            LW   +AF     M +  + ++  K L PGP   P+IGNL +LG+KPH+S+ KL++ +GP+MSL+ G V+TV  S+P   K +L+  D+  C R  P         ++   L + P S  W+ +RK++ + ++T +++ ++Q  R+  +  L+  ++      K +N+ +
Sbjct:    3 LWYIIVAFVFFSSMIIVRIIRKTKKNLPPGPPRLPIIGNLHQLGSKPHRSMFKLSETYGPLMSLKFGSVSTVVASTPETVKEVLKTFDVECCSR--PNMTYPARVTYNLKDLCFSPYSKYWREVRKMTVVELYTAKRVQSFQHTRKEEVAALVDFIKQAASLEKPVNLNK 170          
BLAST of EY649981 vs. ExPASy Swiss-Prot
Match: C71A1_PERAE (Cytochrome P450 71A1 OS=Persea americana GN=CYP71A1 PE=1 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.267e-20
Identity = 53/178 (29.78%), Postives = 98/178 (55.06%), Query Frame = 3
Query:   27 MGLLINCILWLAFTLVWVMALSFVPKRRHKQLAPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGHVTTVWVSSPSMAKAILQNHDLSFCDRMDPETILSLPFPHHEFSLGWLPLSTLWKNLRKISNMHIFTRQKLDAYQELRRTTIKDLLAHVENNCRAGKAINVGQAAL 560
            M +L++ +L+LA  L + +      + +   L P P   P+IGNL +LG  PH+SL  LA   GP++ L LGH+ T+ VS+  +A+ IL+ HDL F  R  P T  +    +    + + P    W+ +RKI  + + + +++++Y+ +R   +  ++  +  +C  G+A+N+ +  L
Sbjct:    1 MAILVS-LLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASR--PSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLL 175          
BLAST of EY649981 vs. ExPASy Swiss-Prot
Match: C71BN_ARATH (Cytochrome P450 71B23 OS=Arabidopsis thaliana GN=CYP71B23 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.655e-20
Identity = 53/144 (36.81%), Postives = 82/144 (56.94%), Query Frame = 3
Query:   45 CILWLAFTLVWVMALSFVPKRRHKQLAPGPRPYPVIGNLLELGAKPHKSLAKLAKIHGPIMSLRLGHVTTVWVSSPSMAKAILQNHDLSFCDRMDPETILSLPFPHHEFSLGWLPLSTLWKNLRKISNMHIFTRQKLDAYQELR 476
            C L L   L+  +  +   +    +L PGP   P+IGNL  L   PHK L  L KIHGP+M L+LG+V  V +SS   A+ +L+ HDL  C R  PETI S    ++   +G+ P    W+ LRK++ + +F+ +K ++++ +R
Sbjct:    6 CFLLLLLLLLVTIIFTRKSQSSKLKLPPGPPKLPIIGNLHYLNGLPHKCLLNLWKIHGPVMQLQLGYVPLVVISSNQAAEEVLKTHDLDCCSR--PETIASKTISYNFKDIGFAPYGEEWRALRKLAVIELFSLKKFNSFRYIR 147          
The following BLAST results are available for this feature:
BLAST of EY649981 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 94
Match NameE-valueIdentityDescription
C76B1_HELTU2.407e-3439.897-ethoxycoumarin O-deethylase OS=Helianthus tubero... [more]
C76C3_ARATH6.811e-2738.93Cytochrome P450 76C3 OS=Arabidopsis thaliana GN=CY... [more]
C76C4_ARATH1.036e-2234.83Cytochrome P450 76C4 OS=Arabidopsis thaliana GN=CY... [more]
F3PH_PETHY1.354e-2233.52Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=C... [more]
C76C1_ARATH3.938e-2232.40Cytochrome P450 76C1 OS=Arabidopsis thaliana GN=CY... [more]
C76C2_ARATH3.334e-2132.75Cytochrome P450 76C2 OS=Arabidopsis thaliana GN=CY... [more]
C71BX_ARATH5.687e-2131.21Cytochrome P450 71B36 OS=Arabidopsis thaliana GN=C... [more]
C71BC_ARATH5.687e-2130.59Cytochrome P450 71B12 OS=Arabidopsis thaliana GN=C... [more]
C71A1_PERAE1.267e-2029.78Cytochrome P450 71A1 OS=Persea americana GN=CYP71A... [more]
C71BN_ARATH1.655e-2036.81Cytochrome P450 71B23 OS=Arabidopsis thaliana GN=C... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-100-005-G10-CT.F Sweet orange leaf, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY649981 ID=EY649981; Name=EY649981; organism=Citrus sinensis; type=EST; length=865bp
CAATTAAAATTCAACTTCAATTCAAGATGGGTTTGTTAATTAACTGCATA
CTGTGGCTTGCATTCACATTGGTTTGGGTGATGGCTTTAAGTTTCGTTCC
CAAACGACGACACAAACAGCTTGCACCGGGACCGAGGCCTTATCCAGTGA
TCGGAAACCTTCTGGAGCTAGGTGCTAAACCTCACAAGTCACTGGCCAAG
CTTGCCAAGATTCATGGGCCCATAATGAGTCTAAGACTTGGCCACGTGAC
CACAGTATGGGTTTCTTCTCCAAGCATGGCCAAAGCCATCCTTCAAAATC
ATGATTTATCATTCTGCGATCGAATGGACCCTGAAACAATCTTGTCACTG
CCCTTCCCGCACCATGAATTCAGCTTGGGCTGGCTTCCACTTTCAACATT
GTGGAAAAATCTTAGAAAAATAAGCAACATGCATATTTTCACTAGGCAGA
AACTCGATGCCTATCAAGAGCTCCGGCGCACGACAATCAAAGACCTTCTT
GCTCATGTTGAAAACAATTGCCGTGCGGGTAAAGCAATAAATGTTGGCCA
AGCTGCTCTCAATACTTGCACTTAATTTATTATCCAACACAATTGGTTCT
ATTGATGTGGCTAATCCCAATGAGCGAGAGTTTAATGATACAGTGTTCTG
GTGTCTTGGAAGAAGCATGATATCCTAACTTGTCCGGCCATTTTACTATT
GTCAGAAGTTTGATCTGCACGTGATAAAGCGCCGCATGACAGTCTATAGT
GCCAAATGCTTCCAGGTGNTTGATCGCTTCGTTGATCAACGATTGCATCA
TANACACGACATGGGGTGCATTGTTAGCAACGATGTAACGAATATTAAAT
ACTCTCTGCATATGA
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