DN621454

Overview
NameDN621454
Unique NameDN621454
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length745
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DN621454 vs. ExPASy Swiss-Prot
Match: VTI12_ARATH (Vesicle transport v-SNARE 12 OS=Arabidopsis thaliana GN=VTI12 PE=1 SV=3)

HSP 1 Score: 186.037 bits (471), Expect = 1.798e-46
Identity = 100/162 (61.73%), Postives = 121/162 (74.69%), Query Frame = -3
Query:  270 RSLQPNVKAMLLAKLREYKSDLNKLKREFKRVXXXXXXXXXXXS----GKADPNVVSGEQRERLAMSVERINQSGERIRESRRVMLETEELGISIVEDLNQQRETLLNSRNKLHGVDDAISKSKKVLSSMSRRMTRNKWIVGSXXXXXXXXXXXXXFYKLAH 743
            RSLQP+ KA+ L+KLREYKSDLN+LK+EFKRV                G AD + VS +QR RLAMSVER++QS +RIRESRR+MLETEE+GISIV+DL+QQR+TLL++ NKLHGVDDAI KSKKVL++MSRRMTRNKWI+ S              YKL+H
Sbjct:   61 RSLQPSAKAVCLSKLREYKSDLNQLKKEFKRVSSADAKPSSREELMESGMADLHAVSADQRGRLAMSVERLDQSSDRIRESRRLMLETEEVGISIVQDLSQQRQTLLHAHNKLHGVDDAIDKSKKVLTAMSRRMTRNKWIITSVIVALVLAIILIISYKLSH 222          
BLAST of DN621454 vs. ExPASy Swiss-Prot
Match: VTI13_ARATH (Vesicle transport v-SNARE 13 OS=Arabidopsis thaliana GN=VTI13 PE=2 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.337e-33
Identity = 72/143 (50.35%), Postives = 98/143 (68.53%), Query Frame = -3
Query:  327 RSLQPNVKAMLLAKLREYKSDLNKLKREFKRVXXXXXXXXXXXS----GKADPNVVSGEQRERLAMSVERINQSGERIRESRRVMLETEELGISIVEDLNQQRETLLNSRNKLHGVDDAISKSKKVLSSMSRRMTRNKWIVGS 743
            R+L PNVK+ LL KLREYKSDLN  K E KR+                G AD    S +QR RL MS + + ++ +RI++SRR +LETEELG+SI++DL+ QR++LL +   LHGVDD + KSKK+L++M+RRM RNKW +G+
Sbjct:   60 RNLPPNVKSSLLVKLREYKSDLNNFKTEVKRITSGNLNATARDELLEAGMADTLTASADQRSRLMMSTDHLGRTTDRIKDSRRTILETEELGVSILQDLHGQRQSLLRAHETLHGVDDNVGKSKKILTTMTRRMNRNKWTIGA 202          
BLAST of DN621454 vs. ExPASy Swiss-Prot
Match: VTI11_ARATH (Vesicle transport v-SNARE 11 OS=Arabidopsis thaliana GN=VTI11 PE=1 SV=2)

HSP 1 Score: 142.895 bits (359), Expect = 1.746e-33
Identity = 70/143 (48.95%), Postives = 98/143 (68.53%), Query Frame = -3
Query:  327 RSLQPNVKAMLLAKLREYKSDLNKLKREFKRVXXXXXXXXXXXS----GKADPNVVSGEQRERLAMSVERINQSGERIRESRRVMLETEELGISIVEDLNQQRETLLNSRNKLHGVDDAISKSKKVLSSMSRRMTRNKWIVGS 743
            R+L PN+K+ LL KLRE+KSDLN  K E KR+                G AD    S +QR RL MS ER+ ++ +R+++SRR M+ETEE+G+SI++DL+ QR++LL +   LHGVDD I KSKK+L+ M+RRM +NKW +G+
Sbjct:   60 RTLPPNLKSSLLVKLREFKSDLNNFKTEVKRITSGQLNAAARDELLEAGMADTKTASADQRARLMMSTERLGRTTDRVKDSRRTMMETEEIGVSILQDLHGQRQSLLRAHETLHGVDDNIGKSKKILTDMTRRMNKNKWTIGA 202          
BLAST of DN621454 vs. ExPASy Swiss-Prot
Match: VTI1B_HUMAN (Vesicle transport through interaction with t-SNAREs homolog 1B OS=Homo sapiens GN=VTI1B PE=1 SV=3)

HSP 1 Score: 74.3294 bits (181), Expect = 7.626e-13
Identity = 44/138 (31.88%), Postives = 74/138 (53.62%), Query Frame = -3
Query:  333 LLAKLREYKSDLNKLKREFKRVXXXXXXXXXXXSGKADPN----VVSGE-------QRERLAMSVERINQSGERIRESRRVMLETEELGISIVEDLNQQRETLLNSRNKLHGVDDAISKSKKVLSSMSRRMTRNKWIV 713
            +++KLR Y+ DL KL RE +              G+ D       V  E       QR  L    E +N++ + I  S R+  ET+++G  I+E+L +QR+ L  ++++L    + +SKS+K+L SMSR++T NK ++
Sbjct:   77 MMSKLRNYRKDLAKLHREVRSTPLTATPG-----GRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLLL 209          
BLAST of DN621454 vs. ExPASy Swiss-Prot
Match: VTI1B_BOVIN (Vesicle transport through interaction with t-SNAREs homolog 1B OS=Bos taurus GN=VTI1B PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.219e-12
Identity = 42/134 (31.34%), Postives = 72/134 (53.73%), Query Frame = -3
Query:  333 LLAKLREYKSDLNKLKREFKRVXXXXXXXXXXXSGKADPNVVSGE-------QRERLAMSVERINQSGERIRESRRVMLETEELGISIVEDLNQQRETLLNSRNKLHGVDDAISKSKKVLSSMSRRMTRNKWIV 713
            +++KLR Y+ DL KL RE +               K     V  E       QR  L    + +N++ + I  S R+  ET+++G  I+E+L +QR+ L  ++++L    + +SKS+K+L SMSR++T NK ++
Sbjct:   77 MMSKLRTYRKDLAKLHREVRSTPLTATPGARGDM-KYGTYAVENEHMNRLQSQRALLLQGTDSLNRATQSIERSHRIAAETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLLL 209          
BLAST of DN621454 vs. ExPASy Swiss-Prot
Match: VTI1_YEAST (t-SNARE VTI1 OS=Saccharomyces cerevisiae GN=VTI1 PE=1 SV=3)

HSP 1 Score: 72.4034 bits (176), Expect = 2.898e-12
Identity = 43/130 (33.08%), Postives = 72/130 (55.38%), Query Frame = -3
Query:  336 KAMLLAKLREYKSDLNK-LKREFKRVXXXXXXXXXXXSGKADPNVVSGEQRERLAMSVERINQSGERIRESRRVMLETEELGISIVEDLNQQRETLLNSRNKLHGVDDAISKSKKVLSSMSRRMTRNKWI 722
            +A   AKLRE+K  +   +KR  + +            G  + + +  +QR++L  +   + +SG+R++++ R+  ETE +G  I+ DL  QRETL N+R  L   D  + KS K L +M+RR+  NK+I
Sbjct:   69 RATYKAKLREWKKTIQSDIKRPLQSLVDSGDRDRLF--GDLNASNIDDDQRQQLLSNHAILQKSGDRLKDASRIANETEGIGSQIMMDLRSQRETLENARQTLFQADSYVDKSIKTLKTMTRRLVANKFI 196          
BLAST of DN621454 vs. ExPASy Swiss-Prot
Match: VTI1B_MOUSE (Vesicle transport through interaction with t-SNAREs homolog 1B OS=Mus musculus GN=Vti1b PE=1 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.898e-12
Identity = 44/156 (28.21%), Postives = 76/156 (48.72%), Query Frame = -3
Query:  267 LLAKLREYKSDLNKLKREFKRVXXXXXXXXXXXSGKADPNVVSGE-------QRERLAMSVERINQSGERIRESRRVMLETEELGISIVEDLNQQRETLLNSRNKLHGVDDAISKSKKVLSSMSRRMTRNKWIVGSXXXXXXXXXXXXXFYKLAHH 713
            +++KLR Y+ DL KL RE +               K     +  E       QR  L    E +N++ + I  S R+  ET+++G  I+E+L +QR+ L  ++++L   ++ +SKS+K+L SMSR++  NK ++               +YK   H
Sbjct:   77 MMSKLRNYRKDLAKLHREVRSTPLTAAPGGRGDL-KYGTYTLENEHLNRLQSQRALLLQGTESLNRATQSIERSHRIATETDQIGTEIIEELGEQRDQLERTKSRLVNTNENLSKSRKILRSMSRKVITNKLLLSVIILLELAILVGLVYYKFFRH 231          
BLAST of DN621454 vs. ExPASy Swiss-Prot
Match: VTI1B_RAT (Vesicle transport through interaction with t-SNAREs homolog 1B OS=Rattus norvegicus GN=Vti1b PE=1 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 3.785e-12
Identity = 44/156 (28.21%), Postives = 76/156 (48.72%), Query Frame = -3
Query:  267 LLAKLREYKSDLNKLKREFKRVXXXXXXXXXXXSGKADPNVVSGE-------QRERLAMSVERINQSGERIRESRRVMLETEELGISIVEDLNQQRETLLNSRNKLHGVDDAISKSKKVLSSMSRRMTRNKWIVGSXXXXXXXXXXXXXFYKLAHH 713
            +++KLR Y+ DL KL RE +               K     +  E       QR  L    E +N++ + I  S R+  ET+++G  I+E+L +QR+ L  ++++L   ++ +SKS+K+L SMSR++  NK ++               +YK   H
Sbjct:   77 MMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDL-KFGTYTLENEHLNRLQSQRALLLQGTESLNRATQSIERSHRIAAETDQIGSEIIEELGEQRDQLERTKSRLVNTNENLSKSRKILRSMSRKVITNKLLLSVIIVLELAILVGLVYYKFFRH 231          
BLAST of DN621454 vs. ExPASy Swiss-Prot
Match: VTI1A_MOUSE (Vesicle transport through interaction with t-SNAREs homolog 1A OS=Mus musculus GN=Vti1a PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.785e-12
Identity = 38/134 (28.36%), Postives = 70/134 (52.24%), Query Frame = -3
Query:  342 RSLQPNVKAMLLAKLREYKSDLNKLKREFKRVXXXXXXXXXXXSGKADPNVVSGEQRERLAMSVERINQSGERIRESRRVMLETEELGISIVEDLNQQRETLLNSRNKLHGVDDAISKSKKVLSSMSRRMTRNK 743
            R + P  + M   ++R YK ++ KL+ +FKR                D    S  QR  L  + ER+ +S  R+    ++ +ETE++G  ++E+L+  RE +  +R++L   D  + KS ++L+ M RR+ +N+
Sbjct:   60 REIPPQSRGMYSNRMRSYKQEMGKLETDFKRSRIAYSDEVRNEL-LGDAGNSSENQRAHLLDNTERLERSSRRLEAGYQIAVETEQIGQEMLENLSHDREKIQRARDRLRDADANLGKSSRILTGMLRRIIQNR 192          
BLAST of DN621454 vs. ExPASy Swiss-Prot
Match: VTI1A_RAT (Vesicle transport through interaction with t-SNAREs homolog 1A OS=Rattus norvegicus GN=Vti1a PE=1 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.878e-11
Identity = 38/140 (27.14%), Postives = 73/140 (52.14%), Query Frame = -3
Query:  342 RSLQPNVKAMLLAKLREYKSDLNKLKREFKR----VXXXXXXXXXXXSGKADPN--VVSGEQRERLAMSVERINQSGERIRESRRVMLETEELGISIVEDLNQQRETLLNSRNKLHGVDDAISKSKKVLSSMSRRMTRNK 743
            R + P  + M   ++R YK ++ KL+ +FKR                +G +  N  +   E+R  L  + ER+ +S  R+    ++ +ETE++G  ++E+L+  RE +  +R +L   D  + KS ++L+ M RR+ +N+
Sbjct:   60 REIPPQSRGMYSNRMRSYKQEMGKLETDFKRSRIAYSDEVRNELLGDAGNSSENQLIKLREERAHLLDNTERLERSSRRLEAGYQIAVETEQIGQEMLENLSHDRERIQRARERLRETDANLGKSSRILTGMLRRIIQNR 199          
The following BLAST results are available for this feature:
BLAST of DN621454 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11
Match NameE-valueIdentityDescription
VTI12_ARATH1.798e-4661.73Vesicle transport v-SNARE 12 OS=Arabidopsis thalia... [more]
VTI13_ARATH1.337e-3350.35Vesicle transport v-SNARE 13 OS=Arabidopsis thalia... [more]
VTI11_ARATH1.746e-3348.95Vesicle transport v-SNARE 11 OS=Arabidopsis thalia... [more]
VTI1B_HUMAN7.626e-1331.88Vesicle transport through interaction with t-SNARE... [more]
VTI1B_BOVIN2.219e-1231.34Vesicle transport through interaction with t-SNARE... [more]
VTI1_YEAST2.898e-1233.08t-SNARE VTI1 OS=Saccharomyces cerevisiae GN=VTI1 P... [more]
VTI1B_MOUSE2.898e-1228.21Vesicle transport through interaction with t-SNARE... [more]
VTI1B_RAT3.785e-1228.21Vesicle transport through interaction with t-SNARE... [more]
VTI1A_MOUSE3.785e-1228.36Vesicle transport through interaction with t-SNARE... [more]
VTI1A_RAT1.878e-1127.14Vesicle transport through interaction with t-SNARE... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS11_06L19_f Parent Washington Navel Orange Scale-Infested Rind cDNA Library UCRCS11 Citrus sinensis cDNA clone CS_WEd0006L19, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DN621454 ID=DN621454; Name=DN621454; organism=Citrus sinensis; type=EST; length=745bp
TTTTTTTTTTTTGATCAGGTGATTCAACTTGTATCATTTAGTTTCACGGG
CTCACAAATAAAAATTTCAAGCAAAGAAATTGAACAATGATAATTGATAA
AGGCAATACATCAGAAATGACATAAGATACAACCAATTATTGATCAAGTA
GATAACATGAAGAAAAATGGGAATATATATCCATTAAATCCCCGGTAACT
TTGTACAACATGCACATAAAGCAACAGAACTTTCCTTGAACTTGAGCATT
TGAAATGGTCAGACTAGTGATGTGCAAGCTTATAAAAGAGGATAAAGATT
ATGGCAATGACAAGAGCTACAATTATTGAGCCAACGATCCATTTGTTCCT
GGTCATCCTCCGTGACATGGAAGATAAAACCTTTTTACTCTTGCTTATGG
CATCATCCACCCCATGAAGCTTGTTACGAGAATTTAACAGAGTTTCACGC
TGTTGATTCAGATCTTCCACAATTGAGATACCAAGCTCCTCTGTCTCTAG
CATGACTCTTCTACTCTCCCTAATTCTTTCACCCGACTGATTTATTCTCT
CAACTGACATGGCCAATCTTTCTCTCTGCTCCCCAGAAACCACATTTGGA
TCGGCTTTTCCAGACTCCAGCAACTCTTCGTGAGCTTCGGATGACGAAAC
TCTTTTAAACTCTCTCTTCAACTTATTCAAATCTGACTTGTATTCTCTTA
ATTTAGCAAGCAGCATAGCCTTTACATTCGGCTGCAAACTTCTTG
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