CX047077

Overview
NameCX047077
Unique NameCX047077
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length900
Libraries
Library NameType
Ruby Orange Developing Seed cDNA Library UCRCS09cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX047077 vs. ExPASy Swiss-Prot
Match: THI4_CITSI (Thiazole biosynthetic enzyme, chloroplastic OS=Citrus sinensis GN=THI1 PE=2 SV=1)

HSP 1 Score: 300.827 bits (769), Expect = 6.835e-81
Identity = 154/164 (93.90%), Postives = 154/164 (93.90%), Query Frame = -2
Query:  408 VAAEDXIXXXXXXXXXVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETAD 899
            VAAED I         VTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETAD
Sbjct:  192 VAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETAD 355          
BLAST of CX047077 vs. ExPASy Swiss-Prot
Match: THI4_ALNGL (Thiazole biosynthetic enzyme, chloroplastic OS=Alnus glutinosa GN=THI4 PE=2 SV=1)

HSP 1 Score: 283.108 bits (723), Expect = 1.475e-75
Identity = 143/164 (87.20%), Postives = 149/164 (90.85%), Query Frame = -2
Query:  408 VAAEDXIXXXXXXXXXVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETAD 899
            VAAED I         VTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVK L+SIGMI+ VPGMKALDMN AEDAIVRLTRE+VPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALK+LG PNALDG+YVGG+HPELILAAADSAE AD
Sbjct:  188 VAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKALGLPNALDGSYVGGIHPELILAAADSAEIAD 351          
BLAST of CX047077 vs. ExPASy Swiss-Prot
Match: THI4_ARATH (Thiazole biosynthetic enzyme, chloroplastic OS=Arabidopsis thaliana GN=THI1 PE=1 SV=1)

HSP 1 Score: 269.24 bits (687), Expect = 2.204e-71
Identity = 136/164 (82.93%), Postives = 144/164 (87.80%), Query Frame = -2
Query:  408 VAAEDXIXXXXXXXXXVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETAD 899
            VAAED I         VTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDGPFGATGVKRLKSIGMI+ VPGMKALDMN+AEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKA  LALK+LG PNA+DGT VG + PEL+LAAADSAET D
Sbjct:  185 VAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNLSPELVLAAADSAETVD 348          
BLAST of CX047077 vs. ExPASy Swiss-Prot
Match: THI41_MAIZE (Thiazole biosynthetic enzyme 1-1, chloroplastic OS=Zea mays GN=THI1-1 PE=2 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 2.360e-65
Identity = 127/158 (80.38%), Postives = 135/158 (85.44%), Query Frame = -2
Query:  426 VAAEDXIXXXXXXXXXVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAAD 899
            VA ED I         VTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRL+ IGMI  VPGMKALDMN+AED IVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALK+LG+PNA+DGT    +  EL++A  D
Sbjct:  191 VAVEDLIVRGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLQDIGMISAVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKALGRPNAVDGTMSPPLREELMIAYKD 348          
BLAST of CX047077 vs. ExPASy Swiss-Prot
Match: THI42_MAIZE (Thiazole biosynthetic enzyme 1-2, chloroplastic OS=Zea mays GN=THI1-2 PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 3.408e-64
Identity = 128/162 (79.01%), Postives = 137/162 (84.57%), Query Frame = -2
Query:  426 VAAEDXIXXXXXXXXXVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHP----ELILAAAD 899
            VA ED I         VTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRL+ IGMI  VPGMKALDMN+AED IVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALK+LG+PNA+DGT +  V P    E ++A+ D
Sbjct:  188 VAVEDLIVRRGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLQDIGMISAVPGMKALDMNAAEDEIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKALGRPNAVDGT-IPEVSPALREEFVIASKD 348          
BLAST of CX047077 vs. ExPASy Swiss-Prot
Match: THI4_FUSSH (Thiazole biosynthetic enzyme, mitochondrial OS=Fusarium solani subsp. phaseoli GN=STI35 PE=2 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 3.212e-38
Identity = 77/115 (66.96%), Postives = 94/115 (81.74%), Query Frame = -2
Query:  504 TNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALK 848
            TNW LVSM+HD QSCMDPN + A +V+S+ GHD P GA  VKRL S+G IE++ GM+ LDMN AEDAIV+ TRE+VPG+IV GME++E+DGA RMGPTFGAM++SG KAA  ALK
Sbjct:  199 TNWTLVSMHHDDQSCMDPNTINAPLVISTTGHDAPMGAFCVKRLVSMGRIEKLGGMRGLDMNVAEDAIVKGTREIVPGLIVGGMELSEVDGANRMGPTFGAMVLSGLKAAEEALK 313          
BLAST of CX047077 vs. ExPASy Swiss-Prot
Match: THI4_FUSOX (Thiazole biosynthetic enzyme, mitochondrial OS=Fusarium oxysporum GN=STI35 PE=2 SV=2)

HSP 1 Score: 157.147 bits (396), Expect = 1.221e-37
Identity = 76/115 (66.09%), Postives = 93/115 (80.87%), Query Frame = -2
Query:  504 TNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALK 848
            TNW LVSM+HD QSCMDPN + A +++S+ GHDGP GA  VKRL S+  IE++ GM+ LDMN AEDAIV+ TRE+VPG+IV GME++E+DGA RMGPTFGAM +SG KAA  ALK
Sbjct:  195 TNWTLVSMHHDDQSCMDPNTINAPLIISTTGHDGPMGAFCVKRLVSMQRIEKLGGMRGLDMNLAEDAIVKGTREIVPGLIVGGMELSEVDGANRMGPTFGAMALSGLKAAEEALK 309          
BLAST of CX047077 vs. ExPASy Swiss-Prot
Match: THI4_ASPOR (Thiazole biosynthetic enzyme, mitochondrial OS=Aspergillus oryzae GN=thiA PE=3 SV=2)

HSP 1 Score: 151.369 bits (381), Expect = 6.697e-36
Identity = 71/114 (62.28%), Postives = 91/114 (79.82%), Query Frame = -2
Query:  504 NWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALK 845
            NW LV+++HD  SCMDPN + A V++S+ GHDGPFGA   KRL S+G ++++ GM+ LDMNSAEDAIV+ TREV  G+I+ GME++EIDG  RMGPTFGAM++SG KAA  ALK
Sbjct:  203 NWTLVTLHHDDHSCMDPNTINAPVIISTTGHDGPFGAFCAKRLVSMGSVDKLGGMRGLDMNSAEDAIVKNTREVTKGLIIGGMELSEIDGFNRMGPTFGAMVLSGVKAAEEALK 316          
BLAST of CX047077 vs. ExPASy Swiss-Prot
Match: THI4_UROFA (Thiazole biosynthetic enzyme, mitochondrial OS=Uromyces fabae GN=THI2 PE=3 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 8.747e-36
Identity = 73/129 (56.59%), Postives = 95/129 (73.64%), Query Frame = -2
Query:  462 TNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVG 848
            TNW LVS+NHD QSCMDP+ + A +V S  GHDGPFGA  VKR+ S G+ E +  M+ LDM  AED I   TRE++PG+IV GME++E DG+ RMGPTFGAM++SG++AA +AL+SL +    +G  VG
Sbjct:  207 TNWTLVSLNHDHQSCMDPSTITAPLVCSFAGHDGPFGAFCVKRVASAGLSEGLGDMRPLDMERAEDHIANKTREILPGLIVGGMELSEFDGSARMGPTFGAMLLSGKRAAEVALQSLDRVKIEEGEVVG 335          
BLAST of CX047077 vs. ExPASy Swiss-Prot
Match: THI2_SCHPO (Thiazole biosynthetic enzyme, mitochondrial OS=Schizosaccharomyces pombe GN=thi2 PE=2 SV=2)

HSP 1 Score: 150.599 bits (379), Expect = 1.142e-35
Identity = 76/128 (59.38%), Postives = 91/128 (71.09%), Query Frame = -2
Query:  465 TNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYV 848
            TNW LVS+NH  QSCMDPN + A +VVS+ GHDGPFGA  VKRL S  ++  +  M+ LDMN AED IV+ TREV PGMIV GME++E DGA RMGPTFG MM SG KAA  AL    +  A++  Y+
Sbjct:  201 TNWTLVSLNHGLQSCMDPNTINAHLVVSATGHDGPFGAFCVKRLASAQLVSNLHDMRPLDMNRAEDLIVKGTREVFPGMIVGGMELSEFDGANRMGPTFGGMMFSGIKAAQEALAIFDERKAVNEKYL 328          
The following BLAST results are available for this feature:
BLAST of CX047077 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 47
Match NameE-valueIdentityDescription
THI4_CITSI6.835e-8193.90Thiazole biosynthetic enzyme, chloroplastic OS=Cit... [more]
THI4_ALNGL1.475e-7587.20Thiazole biosynthetic enzyme, chloroplastic OS=Aln... [more]
THI4_ARATH2.204e-7182.93Thiazole biosynthetic enzyme, chloroplastic OS=Ara... [more]
THI41_MAIZE2.360e-6580.38Thiazole biosynthetic enzyme 1-1, chloroplastic OS... [more]
THI42_MAIZE3.408e-6479.01Thiazole biosynthetic enzyme 1-2, chloroplastic OS... [more]
THI4_FUSSH3.212e-3866.96Thiazole biosynthetic enzyme, mitochondrial OS=Fus... [more]
THI4_FUSOX1.221e-3766.09Thiazole biosynthetic enzyme, mitochondrial OS=Fus... [more]
THI4_ASPOR6.697e-3662.28Thiazole biosynthetic enzyme, mitochondrial OS=Asp... [more]
THI4_UROFA8.747e-3656.59Thiazole biosynthetic enzyme, mitochondrial OS=Uro... [more]
THI2_SCHPO1.142e-3559.38Thiazole biosynthetic enzyme, mitochondrial OS=Sch... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS09_12A04_b Ruby Orange Developing Seed cDNA Library UCRCS09 Citrus sinensis cDNA clone UCRCS09-12A04-A7-1-5.b, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX047077 ID=CX047077; Name=CX047077; organism=Citrus sinensis; type=EST; length=900bp
TTTTTAGCAACAAAAACAGAGTAAGCTCAGATCTTCATCATTGATCTTAA
TCACATCAGCACTGTCCCATTGGCTGATGCCTCAAGTTATAAACATACAA
AGCAAAGAAAAGGGAACAGCCCAAGGGCAGTCTTACACATAATACAGATA
ACAAGGACTAGATACGCAACTGCAAATTCACCCCCGAAAAACTCACAACG
GTTTCTTACAAGAGGAGAAAGAAATTAAAATTAAAGGTTGCATGATGGAC
CGGAAACAAACACTTTATTCACAAAACCTCACAACATCATCTCATTATTC
GCATCAACCATCCATCACAACCACCTAAATACAACTACTTTTTTGCCAGA
GTTTATTTGCATGCACACAGCTGATGAGTGATGATGCCTGTTATTATCAA
ATTAACCATCAGCAGTCTCAGCAGAATCAGCTGCAGCTAAGATCAGCTCA
GGGTGAACACCTCCCACATATGTACCGTCTAATGCATTCGGCTGGCCCAG
TGACTTCAACGCCAAATGCGCAGCCTTCTGCCCCGATATCATCATTGCTC
CAAATGTTGGCCCCATTCTAGGAGCACCATCAATCTCGGCAACTTCCATG
CCGGTAACTATCATACCAGGCACAACCTCCCTGGTCAGCCTCACAATAGC
ATCTTCTGCAGAGTTCATGTCCAGTGCCTTCATTCCAGGCACTTCCTCAA
TCATTCCGATACTCTTCAACCTCTTTACTCCAGTGGCCCCAAAAGGCCCA
TCATGGCCACAAGAGCTCACAACCACCTTCGCTTCCATTACATTGGGATC
CATGCAAGACTGTGTATCGTGATTCATTGACACCAACGCCCAGTTGGTCA
CCACACCACCAACTCTGCCGCCTTTGACTATCANGTCCTCGGCTGCCACT
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