CN188060

Overview
NameCN188060
Unique NameCN188060
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length699
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN188060 vs. ExPASy Swiss-Prot
Match: ALF_ORYSJ (Fructose-bisphosphate aldolase cytoplasmic isozyme OS=Oryza sativa subsp. japonica GN=FBA PE=1 SV=2)

HSP 1 Score: 205.682 bits (522), Expect = 4.112e-56
Identity = 100/115 (86.96%), Postives = 109/115 (94.78%), Query Frame = -3
Query:  290 AEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 634
            AE+TVR LQRT+PAAVPA+VFLSGGQSEEEAT NLNAMN+L TKKPWSLSFSFGRALQQSTLKAW+GK EN++KA+AAFL RCKANSEATLGTYKGDA LGEGA+ESLHVKDYKY
Sbjct:  244 AEYTVRTLQRTVPAAVPAIVFLSGGQSEEEATLNLNAMNKLSTKKPWSLSFSFGRALQQSTLKAWSGKAENIEKARAAFLTRCKANSEATLGTYKGDAVLGEGASESLHVKDYKY 358          

HSP 2 Score: 33.8834 bits (76), Expect = 4.112e-56
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = -2
Query:  636 PNMVTPGSEAPKVTPDVI 689
            PNMVTPGS+A KV+P+VI
Sbjct:  226 PNMVTPGSDAKKVSPEVI 243          
BLAST of CN188060 vs. ExPASy Swiss-Prot
Match: ALF_CICAR (Fructose-bisphosphate aldolase, cytoplasmic isozyme OS=Cicer arietinum GN=ALDC PE=2 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 4.472e-55
Identity = 101/123 (82.11%), Postives = 111/123 (90.24%), Query Frame = -3
Query:  290 PKLHRT*FAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 658
            PK+     AEHTVRALQRT+PAAVPAVVFLSGGQSEEEAT NLNA+NQ+K KKPW+LSFSFGRALQQSTLKAW+GKEENV+ AQ A L R KANSEATLGTYKG++QLGEGA+ESLHVKDYKY
Sbjct:  237 PKVAPEVVAEHTVRALQRTVPAAVPAVVFLSGGQSEEEATVNLNAINQVKGKKPWTLSFSFGRALQQSTLKAWSGKEENVKNAQDALLTRAKANSEATLGTYKGNSQLGEGASESLHVKDYKY 359          

HSP 2 Score: 35.039 bits (79), Expect = 4.472e-55
Identity = 13/18 (72.22%), Postives = 17/18 (94.44%), Query Frame = -2
Query:  636 PNMVTPGSEAPKVTPDVI 689
            PNMVTPGS++PKV P+V+
Sbjct:  227 PNMVTPGSDSPKVAPEVV 244          
BLAST of CN188060 vs. ExPASy Swiss-Prot
Match: ALF2_PEA (Fructose-bisphosphate aldolase, cytoplasmic isozyme 2 OS=Pisum sativum PE=3 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 8.985e-53
Identity = 97/123 (78.86%), Postives = 106/123 (86.18%), Query Frame = -3
Query:  290 PKLHRT*FAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 658
            PK+     AEHTVRALQRT+PAAVPAVVFLSGGQSEEEA+ NLNA+NQ+K KKPW+LSFSFGRALQQSTLKAW GK ENV+ AQ A L R KANSEATLGTYKG + LG GA+ESLHVKDYKY
Sbjct:  237 PKVAPEVIAEHTVRALQRTVPAAVPAVVFLSGGQSEEEASVNLNAINQIKGKKPWTLSFSFGRALQQSTLKAWGGKTENVKAAQDALLTRAKANSEATLGTYKGASNLGAGASESLHVKDYKY 359          

HSP 2 Score: 36.5798 bits (83), Expect = 8.985e-53
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = -2
Query:  636 PNMVTPGSEAPKVTPDVI 689
            PNMVTPGS+APKV P+VI
Sbjct:  227 PNMVTPGSDAPKVAPEVI 244          
BLAST of CN188060 vs. ExPASy Swiss-Prot
Match: ALF_MAIZE (Fructose-bisphosphate aldolase, cytoplasmic isozyme OS=Zea mays PE=2 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 2.163e-51
Identity = 96/115 (83.48%), Postives = 103/115 (89.57%), Query Frame = -3
Query:  290 AEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 634
            AE+TVR LQRT+PAAVPAV+FLSGGQSEEEATRNLNAMN+L TKKPWSLSFSFGRALQ STLKAWAGK EN++KA+AAFL RCKANSEATLGTYKGDA       ESLHVKDYKY
Sbjct:  244 AEYTVRTLQRTVPAAVPAVLFLSGGQSEEEATRNLNAMNKLSTKKPWSLSFSFGRALQASTLKAWAGKVENLEKARAAFLARCKANSEATLGTYKGDA---AADTESLHVKDYKY 355          

HSP 2 Score: 34.2686 bits (77), Expect = 2.163e-51
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = -2
Query:  636 PNMVTPGSEAPKVTPDVI 689
            PNMVTPGS++ KVTP+VI
Sbjct:  226 PNMVTPGSDSKKVTPEVI 243          
BLAST of CN188060 vs. ExPASy Swiss-Prot
Match: ALF_SPIOL (Fructose-bisphosphate aldolase, cytoplasmic isozyme OS=Spinacia oleracea PE=2 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 3.918e-47
Identity = 89/115 (77.39%), Postives = 98/115 (85.22%), Query Frame = -3
Query:  290 AEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 634
            AE+TVR LQRT+P AVP V+FLSGGQSEEEAT NLNAMN+L+TKKPW+LSFS+GRALQQSTLKAW GKEENV KAQ  FL R K NSEATLG Y+G A  G  A+ESLHVKDYKY
Sbjct:  244 AEYTVRTLQRTVPQAVPGVMFLSGGQSEEEATLNLNAMNKLETKKPWTLSFSYGRALQQSTLKAWQGKEENVAKAQEVFLARAKGNSEATLGKYQGGAG-GADASESLHVKDYKY 357          

HSP 2 Score: 33.8834 bits (76), Expect = 3.918e-47
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = -2
Query:  636 PNMVTPGSEAPKVTPDVI 689
            PN+VTPGSE+ KVTP+VI
Sbjct:  226 PNIVTPGSESKKVTPEVI 243          
BLAST of CN188060 vs. ExPASy Swiss-Prot
Match: ALF1_PEA (Fructose-bisphosphate aldolase, cytoplasmic isozyme 1 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 1.226e-45
Identity = 86/123 (69.92%), Postives = 98/123 (79.67%), Query Frame = -3
Query:  290 PKLHRT*FAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 658
            PK+      E+TV AL+RT+PAAVP +VFLSGGQSEE+AT NLNAMN+    KPW+LSFSFGRALQQSTLK W+GK+ENV KAQ  FL RCKANSEATLG Y G +  G  A+ESLHVKDYKY
Sbjct:  236 PKVSPEVIGEYTVNALRRTVPAAVPGIVFLSGGQSEEQATLNLNAMNKFDVVKPWTLSFSFGRALQQSTLKTWSGKKENVGKAQDVFLARCKANSEATLGKYGGGSGTGL-ASESLHVKDYKY 357          

HSP 2 Score: 35.8094 bits (81), Expect = 1.226e-45
Identity = 14/18 (77.78%), Postives = 18/18 (100.00%), Query Frame = -2
Query:  636 PNMVTPGSEAPKVTPDVI 689
            PNMVTPGS++PKV+P+VI
Sbjct:  226 PNMVTPGSDSPKVSPEVI 243          
BLAST of CN188060 vs. ExPASy Swiss-Prot
Match: ALF_ARATH (Fructose-bisphosphate aldolase, cytoplasmic isozyme OS=Arabidopsis thaliana GN=At4g26520 PE=2 SV=2)

HSP 1 Score: 147.517 bits (371), Expect = 7.431e-39
Identity = 76/123 (61.79%), Postives = 90/123 (73.17%), Query Frame = -3
Query:  290 PKLHRT*FAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 658
            PK+     AE+TV AL+RT+P A+P +VFLSG Q EE+AT NLNAMN+L   KPW+L+FSFG ALQQS +KAWAGK ENV KAQA FL RCKAN +ATLG Y G A     A E+L V  Y+Y
Sbjct:  236 PKVAPELIAEYTVTALRRTVPPAIPGIVFLSGIQREEQATLNLNAMNKLDVLKPWTLTFSFGGALQQSAIKAWAGKPENVAKAQAKFLTRCKANKDATLGKYTGWASGDSAAFENLVVIGYRY 358          

HSP 2 Score: 34.2686 bits (77), Expect = 7.431e-39
Identity = 13/18 (72.22%), Postives = 17/18 (94.44%), Query Frame = -2
Query:  636 PNMVTPGSEAPKVTPDVI 689
            PNMVTPGS++PKV P++I
Sbjct:  226 PNMVTPGSDSPKVAPELI 243          
BLAST of CN188060 vs. ExPASy Swiss-Prot
Match: ALF1_CAEEL (Fructose-bisphosphate aldolase 1 OS=Caenorhabditis elegans GN=aldo-1 PE=1 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 9.517e-31
Identity = 66/112 (58.93%), Postives = 83/112 (74.11%), Query Frame = -3
Query:  290 TVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 625
            TV ALQR +P+AVP VVFLSGGQSEE+AT NLNA+NQ+  KKPW+L+FS+GRALQ S L  WAGK+EN+  AQ   L R + NS A++G Y GDA     A++SL V ++ Y
Sbjct:  255 TVTALQRGVPSAVPGVVFLSGGQSEEDATLNLNAINQVSGKKPWALTFSYGRALQASCLAKWAGKDENIAAAQEVLLHRAQVNSLASVGKYTGDASADAAASQSLFVANHSY 366          
BLAST of CN188060 vs. ExPASy Swiss-Prot
Match: ALF_ECHMU (Fructose-bisphosphate aldolase OS=Echinococcus multilocularis GN=FBPA PE=2 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 8.057e-30
Identity = 68/115 (59.13%), Postives = 83/115 (72.17%), Query Frame = -3
Query:  290 AEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 634
            A  TV ALQRT+P AVP VVFLSGGQSE +ATRNLNA+N+   KKPW+LSFSFGRALQ S + AW GK ENV+  QA FL   KAN  A+LG ++G+ +   G  +SL V ++ Y
Sbjct:  250 ARATVEALQRTVPVAVPGVVFLSGGQSELDATRNLNAINKYPGKKPWALSFSFGRALQASAIAAWQGKPENVKAGQAEFLQLAKANGAASLGKFEGELKTAAG-QKSLFVANHAY 363          
BLAST of CN188060 vs. ExPASy Swiss-Prot
Match: ALDOB_DANRE (Fructose-bisphosphate aldolase B OS=Danio rerio GN=aldob PE=2 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.374e-29
Identity = 64/112 (57.14%), Postives = 78/112 (69.64%), Query Frame = -3
Query:  290 TVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 625
            TV AL+RT+PAAVP + FLSGGQSEEEA+ NLNAMNQL   +PW LSFS+GRALQ S L AW G+  N + +Q AF+ R K NS A+ G YK   Q G+ + +SL    Y Y
Sbjct:  253 TVTALRRTVPAAVPGICFLSGGQSEEEASLNLNAMNQLSLHRPWKLSFSYGRALQASALSAWKGQAANKKASQDAFVTRAKINSLASKGEYKPSGQAGQASTQSLFTASYTY 364          
The following BLAST results are available for this feature:
BLAST of CN188060 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 58
Match NameE-valueIdentityDescription
ALF_ORYSJ4.112e-5686.96Fructose-bisphosphate aldolase cytoplasmic isozyme... [more]
ALF_CICAR4.472e-5582.11Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF2_PEA8.985e-5378.86Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF_MAIZE2.163e-5183.48Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF_SPIOL3.918e-4777.39Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF1_PEA1.226e-4569.92Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF_ARATH7.431e-3961.79Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF1_CAEEL9.517e-3158.93Fructose-bisphosphate aldolase 1 OS=Caenorhabditis... [more]
ALF_ECHMU8.057e-3059.13Fructose-bisphosphate aldolase OS=Echinococcus mul... [more]
ALDOB_DANRE1.374e-2957.14Fructose-bisphosphate aldolase B OS=Danio rerio GN... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0005H01_f Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005H01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188060 ID=CN188060; Name=CN188060; organism=Citrus sinensis; type=EST; length=699bp
CCCCTTTTTTTAGCACAGAAACATTATCAATTATGTGACAAAATCACAGA
AATTCAGTGCCATTAGGGGAAAGGAAAAAAAAAGAAAAACAAAAACAAAA
GCATAAACAGTTTTCAGTTTAGTTTTGATCAAAACCACTCAAACCGAAGT
TCCCAAGCTTTTTATTGCTCCACCTAAATATGAAAAAGCTCTAAAACTAT
GAATGAGAATGGGCCAACCTAATGTTCATACCGCCATTTACTCTCATAAA
GTTCATAAAGCACAACACCCAAAGCTTCAACTGTGCTTAGTATTTGTAGT
CCTTGACATGGAGAGACTCTGCGGCACCTTCACCAAGCTGGGCATCACCC
TTGTAAGTACCGAGGGTAGCCTCAGAGTTGGCCTTGCACCTGACAAGGAA
AGCAGCCTGGGCCTTCTGCACATTCTCTTCCTTTCCAGCCCATGCCTTGA
GAGTGCTCTGCTGAAGTGCACGTCCAAAGGAGAAGGAAAGTGACCATGGC
TTCTTCGTCTTGAGCTGGTTCATGGCATTAAGGTTGCGGGTAGCCTCCTC
CTCACTCTGCCCACCAGACAAGAACACAACAGCAGGAACTGCTGCAGGCA
TAGTGCGTTGCAGGGCCCGGACAGTGTGTTCAGCAAATCACGTCCGGTGT
AACTTTGGGAGCTTCAGATCCAGGGGTCACCATGTTGGGGCTTCAAAAG
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