EY665822

Overview
NameEY665822
Unique NameEY665822
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length643
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY665822 vs. ExPASy Swiss-Prot
Match: HAL3A_ARATH (Phosphopantothenoylcysteine decarboxylase OS=Arabidopsis thaliana GN=HAL3A PE=1 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 2.069e-58
Identity = 105/124 (84.68%), Postives = 114/124 (91.94%), Query Frame = 2
Query:   26 QIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYNKPLFVAPAMNTFMWNNPFTERHLMSIDELGISLIPPVSKRLACGDYGNGAMAEPSLIYSTVRLFAESRNQSGDG 397
            +IGD VLHIELRRWAD++VIAPLSANTLGKIAGGLCDNLLTCI+RAWDY KPLFVAPAMNT MWNNPFTERHL+S+DELGI+LIPP+ KRLACGDYGNGAMAEPSLIYSTVRLF ES+     G
Sbjct:   83 KIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDNLLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLACGDYGNGAMAEPSLIYSTVRLFWESQAHQQTG 206          
BLAST of EY665822 vs. ExPASy Swiss-Prot
Match: HAL3B_ARATH (Probable phosphopantothenoylcysteine decarboxylase OS=Arabidopsis thaliana GN=HAL3B PE=2 SV=2)

HSP 1 Score: 220.32 bits (560), Expect = 6.655e-57
Identity = 102/118 (86.44%), Postives = 112/118 (94.92%), Query Frame = 2
Query:   26 QIGDSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYNKPLFVAPAMNTFMWNNPFTERHLMSIDELGISLIPPVSKRLACGDYGNGAMAEPSLIYSTVRLFAESR 379
            +IGD VLHIELRRWAD+M+IAPLSANTL KIAGGLCDNLLTCIVRAWDY+KPLFVAPAMNT MWNNPFTERHL+ +DELGI+LIPP+ K+LACGDYGNGAMAEPSLIYSTVRLF ES+
Sbjct:   75 KIGDPVLHIELRRWADVMIIAPLSANTLAKIAGGLCDNLLTCIVRAWDYSKPLFVAPAMNTLMWNNPFTERHLVLLDELGITLIPPIKKKLACGDYGNGAMAEPSLIYSTVRLFWESQ 192          
BLAST of EY665822 vs. ExPASy Swiss-Prot
Match: COAC_MOUSE (Phosphopantothenoylcysteine decarboxylase OS=Mus musculus GN=Ppcdc PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 7.916e-34
Identity = 65/109 (59.63%), Postives = 80/109 (73.39%), Query Frame = 2
Query:   35 DSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYNKPLFVAPAMNTFMWNNPFTERHLMSIDELGISLIPPVSKRLACGDYGNGAMAEPSLIYSTVR 361
            D VLHI+LRRWAD+M++APL ANTLGK+A G+CDNLLTC++RAWD NKPL   PAMNT MW +P T + +  +   G   IP VSK+L CGD G GAMAE   I + V+
Sbjct:   84 DPVLHIDLRRWADLMLVAPLDANTLGKVASGICDNLLTCVIRAWDLNKPLLFCPAMNTAMWEHPLTAQQVAQLKAFGYVEIPCVSKKLVCGDQGLGAMAEVETIVAKVQ 192          
BLAST of EY665822 vs. ExPASy Swiss-Prot
Match: COAC_HUMAN (Phosphopantothenoylcysteine decarboxylase OS=Homo sapiens GN=PPCDC PE=1 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 7.409e-32
Identity = 62/109 (56.88%), Postives = 79/109 (72.48%), Query Frame = 2
Query:   35 DSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYNKPLFVAPAMNTFMWNNPFTERHLMSIDELGISLIPPVSKRLACGDYGNGAMAEPSLIYSTVR 361
            D VLHI+LRRWAD++++APL ANTLGK+A G+CDNLLTC++RAWD +KPL   PAMNT MW +P T + +  +   G   IP V+K+L CGD G GAMAE   I   V+
Sbjct:   84 DPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVK 192          
BLAST of EY665822 vs. ExPASy Swiss-Prot
Match: COAC_DICDI (Putative phosphopantothenoylcysteine decarboxylase OS=Dictyostelium discoideum GN=ppcdc PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.308e-28
Identity = 59/111 (53.15%), Postives = 82/111 (73.87%), Query Frame = 2
Query:   38 SVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDY-NKPLFVAPAMNTFMWNNPFTERHLMSIDEL--GISLIPPVSKRLACGDYGNGAMAE-PSLIYSTV 358
            S LHI+LR WA+ ++I+P SANTLGKI+ GLCDNLLT ++RAWDY NK + +APAMNT MW NPFT +H+ ++  +   + +I P+ K+L CGD G GAM + P ++  T+
Sbjct:   83 SALHIDLRNWANSILISPCSANTLGKISNGLCDNLLTSLIRAWDYKNKSMILAPAMNTMMWENPFTFKHIETLKSISPNVFIIDPIEKKLFCGDIGMGAMEQVPKIVDFTI 193          
BLAST of EY665822 vs. ExPASy Swiss-Prot
Match: TYSY_SCHPO (Probable thymidylate synthase OS=Schizosaccharomyces pombe GN=SPAC15E1.04 PE=2 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 2.913e-28
Identity = 58/100 (58.00%), Postives = 72/100 (72.00%), Query Frame = 2
Query:   41 VLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYNKPLFVAPAMNTFMWNNPFTERHLMSIDEL--GISLIPPVSKRLACGDYGNGAMAE 334
            + HIELRRWA +++IAPLSANT+ K+A GLCDNLLT ++RAW   KP+ +APAMNT MW NP T+ HL +I  +      I P+ K LACGD G G MAE
Sbjct:  102 ITHIELRRWAHLLLIAPLSANTMAKMANGLCDNLLTSLIRAWAPLKPILLAPAMNTLMWTNPITQEHLSAISRIYKNSEFIMPIEKVLACGDIGMGGMAE 201          
BLAST of EY665822 vs. ExPASy Swiss-Prot
Match: YKI8_YEAST (Uncharacterized protein YKL088W OS=Saccharomyces cerevisiae GN=YKL088W PE=1 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 2.727e-26
Identity = 61/124 (49.19%), Postives = 81/124 (65.32%), Query Frame = 2
Query:   41 VLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYNKPLFVAPAMNTFMWNNPFTERHLMSI--DELGISLIPPVSKRLACGDYGNGAMAEPSLIYSTVRLFAESRNQSGDGKVG 406
            +LH ELR+WADI +IAPLSANTL K+A G+C+NLLT ++R W    P+ +APAMNTFM+ NP T++HL S+  D   I ++ PV K L CGD G G M E + I   VR       ++ D + G
Sbjct:  389 ILHHELRKWADIFLIAPLSANTLAKLANGICNNLLTSVMRDWSPLTPVLIAPAMNTFMYINPMTKKHLTSLVQDYPFIQVLKPVEKVLICGDIGMGGMREWTDIVEIVRRRINEIRKARDEETG 512          
BLAST of EY665822 vs. ExPASy Swiss-Prot
Match: VHS3_YEAST (Protein VHS3 OS=Saccharomyces cerevisiae GN=VHS3 PE=1 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.311e-20
Identity = 52/111 (46.85%), Postives = 73/111 (65.77%), Query Frame = 2
Query:   35 DSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYNKPLFVAPAMNTFMWNNPFTERHLMSIDE--LGISLIPPVSKRLAC-GDYGNGAMAEPSLIYSTV 358
            D VLHIELRRWADI+V+APL+ANTL KIA GLCDNLLT ++RAW+   P+F+AP+M +  +N+  T++H   I E    +++  P  K +   GD G   M + + I   +
Sbjct:  455 DPVLHIELRRWADILVVAPLTANTLAKIALGLCDNLLTSVIRAWNPTFPIFLAPSMGSGTFNSIMTKKHFRIIQEEMPWVTVFKPSEKVMGINGDIGLSGMMDANEIVGKI 565          
BLAST of EY665822 vs. ExPASy Swiss-Prot
Match: SIS2_YEAST (Protein SIS2 OS=Saccharomyces cerevisiae GN=SIS2 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.920e-20
Identity = 51/103 (49.51%), Postives = 72/103 (69.90%), Query Frame = 2
Query:   35 DSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYNKPLFVAPAMNTFMWNNPFTERHLMSIDE--LGISLIPPVSKRLAC-GDYGNGAMAE 334
            D VLHIELRRWADI+V+APL+ANTL KIA GLCDNLLT ++RAW+ + P+ +AP+M +  +N+  T++ L +I E    +++  P  K +   GD G G M +
Sbjct:  374 DPVLHIELRRWADILVVAPLTANTLSKIALGLCDNLLTSVIRAWNPSYPILLAPSMVSSTFNSMMTKKQLQTIKEEMSWVTVFKPSEKVMDINGDIGLGGMMD 476          
BLAST of EY665822 vs. ExPASy Swiss-Prot
Match: SIS2_CANTR (Protein SIS2 OS=Candida tropicalis GN=SIS2 PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.110e-19
Identity = 49/103 (47.57%), Postives = 72/103 (69.90%), Query Frame = 2
Query:   35 DSVLHIELRRWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYNKPLFVAPAMNTFMWNNPFTERHLMSI--DELGISLIPPVSKRL-ACGDYGNGAMAE 334
            D VLHIELRRWADI+++ PL+ANTL KI+ G+CDNLLT ++RAW+ + P+ +APAM++  +++  T+R L  I  D   I ++ P+ K   + GD G G M +
Sbjct:  344 DPVLHIELRRWADILLVCPLTANTLAKISLGICDNLLTNVIRAWNSSYPILLAPAMDSHSYSSSTTKRQLRLIADDMPWIEVLKPLEKVFGSYGDIGMGGMTD 446          
The following BLAST results are available for this feature:
BLAST of EY665822 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 21
Match NameE-valueIdentityDescription
HAL3A_ARATH2.069e-5884.68Phosphopantothenoylcysteine decarboxylase OS=Arabi... [more]
HAL3B_ARATH6.655e-5786.44Probable phosphopantothenoylcysteine decarboxylase... [more]
COAC_MOUSE7.916e-3459.63Phosphopantothenoylcysteine decarboxylase OS=Mus m... [more]
COAC_HUMAN7.409e-3256.88Phosphopantothenoylcysteine decarboxylase OS=Homo ... [more]
COAC_DICDI1.308e-2853.15Putative phosphopantothenoylcysteine decarboxylase... [more]
TYSY_SCHPO2.913e-2858.00Probable thymidylate synthase OS=Schizosaccharomyc... [more]
YKI8_YEAST2.727e-2649.19Uncharacterized protein YKL088W OS=Saccharomyces c... [more]
VHS3_YEAST1.311e-2046.85Protein VHS3 OS=Saccharomyces cerevisiae GN=VHS3 P... [more]
SIS2_YEAST2.920e-2049.51Protein SIS2 OS=Saccharomyces cerevisiae GN=SIS2 P... [more]
SIS2_CANTR1.110e-1947.57Protein SIS2 OS=Candida tropicalis GN=SIS2 PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-102-095-H12-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY665822 ID=EY665822; Name=EY665822; organism=Citrus sinensis; type=EST; length=643bp
ACGAGGATGAATGGGCCACCTTTAACAAATAGGTGATAGCGTTCTTCACA
TCGAGCTCCGCAGATGGGCTGATATTATGGTTATTGCTCCGCTGTCAGCA
AACACACTTGGCAAGATTGCAGGTGGATTGTGTGACAATCTGCTGACATG
CATTGTGCGGGCATGGGACTACAATAAGCCACTGTTTGTTGCACCTGCTA
TGAACACTTTCATGTGGAATAATCCATTCACAGAGCGGCATCTTATGTCA
ATTGATGAGCTTGGAATTTCTCTCATTCCACCTGTCTCCAAAAGGCTGGC
TTGCGGAGATTATGGTAATGGTGCCATGGCTGAACCTTCATTGATCTACT
CGACTGTGAGACTGTTCGCAGAGTCAAGGAACCAGTCAGGCGATGGAAAA
GTTGGTTAATCCAGTCCAGTTTGCATGGAATGCTAAATTTCTTGTTGTAG
TCCATTGTCCCCGGATTAAGTGACAAACGCTATATTGAAACTATATATGT
CATCTGAGAGTGTTCTGATCTACTTTTGTAATCATGTACGATATTGCCCG
TTAACCTTTTGGAAGTGAAATGTGGAGGCCATCCTCCAATCTGTTCCCCA
TGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG
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