EY665743

Overview
NameEY665743
Unique NameEY665743
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length816
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY665743 vs. ExPASy Swiss-Prot
Match: ASPRX_ORYSJ (Aspartic proteinase OS=Oryza sativa subsp. japonica GN=RAP PE=2 SV=2)

HSP 1 Score: 139.428 bits (350), Expect = 3.657e-40
Identity = 59/98 (60.20%), Postives = 82/98 (83.67%), Query Frame = 3
Query:  264 KLCEVLPNPMGKSFINCDDIASMPYVSFTIGNRSFPLSPEQYIFKIEEGHSTICISGFIALDVPPPQGPLWVLGDMFLRAYHTVFDFGNLQIGFAETA 557
            +LCE LP+P G+S ++C  I+ MP ++FTI N++F L+PEQYI K+E+G  T+CISGF+A D+PPP+GPLW+LGD+F+ AYHTVFDFG  +IGFA++A
Sbjct:  399 QLCERLPSPNGESTVSCHQISKMPNLAFTIANKTFILTPEQYIVKLEQGGQTVCISGFMAFDIPPPRGPLWILGDVFMGAYHTVFDFGKDRIGFAKSA 496          

HSP 2 Score: 47.3654 bits (111), Expect = 3.657e-40
Identity = 22/53 (41.51%), Postives = 33/53 (62.26%), Query Frame = 1
Query:   85 STGIETVVQHKTSNGSSINESTLCAFCEMIVFWIQMQLKQQKTKEAIFKYADK 243
            S GIE+VV  +     ++    +C+ CEM V WI+ QL++ KTKE I  YA++
Sbjct:  352 SNGIESVVDKE-----NLGSDAMCSVCEMAVVWIENQLRENKTKELILNYANQ 399          
BLAST of EY665743 vs. ExPASy Swiss-Prot
Match: ASPR1_ORYSJ (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 8.085e-40
Identity = 57/98 (58.16%), Postives = 81/98 (82.65%), Query Frame = 3
Query:  264 KLCEVLPNPMGKSFINCDDIASMPYVSFTIGNRSFPLSPEQYIFKIEEGHSTICISGFIALDVPPPQGPLWVLGDMFLRAYHTVFDFGNLQIGFAETA 557
            +LC+ LP+PMG+S ++C  +ASMP +SFTIG + F L PE+YI K+ EG +  CISGF A+D+PPP+GPLW+LGD+F+ AYHTVFD+G +++GFA++A
Sbjct:  412 QLCDKLPSPMGESSVDCGSLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGAYHTVFDYGKMRVGFAKSA 509          

HSP 2 Score: 45.4394 bits (106), Expect = 8.085e-40
Identity = 19/53 (35.85%), Postives = 30/53 (56.60%), Query Frame = 1
Query:   85 STGIETVVQHKTSNGSSINESTLCAFCEMIVFWIQMQLKQQKTKEAIFKYADK 243
            S GI++VV  +    + +    +C  CEM V W+Q QL Q KT++ I  Y ++
Sbjct:  360 SAGIKSVVDDEAGESNGLQSGPMCNACEMAVVWMQNQLAQNKTQDLILNYINQ 412          
BLAST of EY665743 vs. ExPASy Swiss-Prot
Match: ASPR_CUCPE (Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 6.711e-39
Identity = 57/98 (58.16%), Postives = 77/98 (78.57%), Query Frame = 3
Query:  264 KLCEVLPNPMGKSFINCDDIASMPYVSFTIGNRSFPLSPEQYIFKIEEGHSTICISGFIALDVPPPQGPLWVLGDMFLRAYHTVFDFGNLQIGFAETA 557
            +LC+ +P+PMG+S ++C  ++SMP VSFTIG + F L+PE+YI K+ EG    CISGF A D+PPP+GPLW+LGD+F+  YHTVFDFG L++G AE A
Sbjct:  416 ELCDRMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGDVFMGRYHTVFDFGKLRVGSAEAA 513          

HSP 2 Score: 48.521 bits (114), Expect = 6.711e-39
Identity = 22/53 (41.51%), Postives = 30/53 (56.60%), Query Frame = 1
Query:   85 STGIETVVQHKTSNGSSINESTLCAFCEMIVFWIQMQLKQQKTKEAIFKYADK 243
            S GIE+VV       S      +C+ CEM V W+Q QL+Q +TKE I  Y ++
Sbjct:  364 SMGIESVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINE 416          
BLAST of EY665743 vs. ExPASy Swiss-Prot
Match: ASPR_HORVU (Phytepsin OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 7.256e-38
Identity = 56/98 (57.14%), Postives = 76/98 (77.55%), Query Frame = 3
Query:  264 KLCEVLPNPMGKSFINCDDIASMPYVSFTIGNRSFPLSPEQYIFKIEEGHSTICISGFIALDVPPPQGPLWVLGDMFLRAYHTVFDFGNLQIGFAETA 557
            +LC  LP+PMG+S ++C  + SMP + FTIG + F L PE+YI K+ EG +  CISGF A+D+PPP+GPLW+LGD+F+  YHTVFD+G L+IGFA+ A
Sbjct:  411 QLCNRLPSPMGESAVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGPYHTVFDYGKLRIGFAKAA 508          

HSP 2 Score: 44.669 bits (104), Expect = 7.256e-38
Identity = 19/53 (35.85%), Postives = 30/53 (56.60%), Query Frame = 1
Query:   85 STGIETVVQHKTSNGSSINESTLCAFCEMIVFWIQMQLKQQKTKEAIFKYADK 243
            S GI +VV  +    + +    +C+ CEM V W+Q QL Q KT++ I  Y ++
Sbjct:  359 SAGIRSVVDDEPVKSNGLRADPMCSACEMAVVWMQNQLAQNKTQDLILDYVNQ 411          
BLAST of EY665743 vs. ExPASy Swiss-Prot
Match: CYPR1_CYNCA (Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO1 PE=1 SV=2)

HSP 1 Score: 141.739 bits (356), Expect = 1.606e-37
Identity = 59/98 (60.20%), Postives = 80/98 (81.63%), Query Frame = 3
Query:  264 KLCEVLPNPMGKSFINCDDIASMPYVSFTIGNRSFPLSPEQYIFKIEEGHSTICISGFIALDVPPPQGPLWVLGDMFLRAYHTVFDFGNLQIGFAETA 557
            KLCE LP+PMG+S ++C  ++SMP ++FT+G ++F LSPEQY+ K+ EG +  CISGF A+DV PP GPLW+LGD+F+  YHTVFD+GNL++GFAE A
Sbjct:  376 KLCERLPSPMGESAVDCSSLSSMPNIAFTVGGKTFNLSPEQYVLKVGEGATAQCISGFTAMDVAPPHGPLWILGDVFMGQYHTVFDYGNLRVGFAEAA 473          

HSP 2 Score: 36.1946 bits (82), Expect = 1.606e-37
Identity = 20/50 (40.00%), Postives = 26/50 (52.00%), Query Frame = 1
Query:   94 IETVVQHKTSNGSSINESTLCAFCEMIVFWIQMQLKQQKTKEAIFKYADK 243
            IE+VV    S G S      C  C   V W+Q Q++Q +T+E I  Y DK
Sbjct:  329 IESVVDK--SKGKSSGLPMRCVPCARWVVWMQNQIRQNETEENIINYVDK 376          
BLAST of EY665743 vs. ExPASy Swiss-Prot
Match: CATD_RAT (Cathepsin D OS=Rattus norvegicus GN=Ctsd PE=1 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 4.712e-22
Identity = 43/100 (43.00%), Postives = 69/100 (69.00%), Query Frame = 3
Query:  258 LFKLCEVLPNPMGKSFINCDDIASMPYVSFTIGNRSFPLSPEQYIFKIEEGHSTICISGFIALDVPPPQGPLWVLGDMFLRAYHTVFDFGNLQIGFAETA 557
            L K    +P   G+  I C+ ++S+P ++F +G +++ L PE+YI K+ +   TIC+SGF+ +D+PPP GPLW+LGD+F+  Y+TVFD    ++GFA+ A
Sbjct:  306 LQKAIGAVPLIQGEYMIPCEKVSSLPIITFKLGGQNYELHPEKYILKVSQAGKTICLSGFMGMDIPPPSGPLWILGDVFIGCYYTVFDREYNRVGFAKAA 405          
BLAST of EY665743 vs. ExPASy Swiss-Prot
Match: CATD_MOUSE (Cathepsin D OS=Mus musculus GN=Ctsd PE=1 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 4.712e-22
Identity = 43/97 (44.33%), Postives = 68/97 (70.10%), Query Frame = 3
Query:  258 LFKLCEVLPNPMGKSFINCDDIASMPYVSFTIGNRSFPLSPEQYIFKIEEGHSTICISGFIALDVPPPQGPLWVLGDMFLRAYHTVFDFGNLQIGFA 548
            L K    +P   G+  I C+ ++S+P V   +G +++ L P++YI K+ +G  TIC+SGF+ +D+PPP GPLW+LGD+F+ +Y+TVFD  N ++GFA
Sbjct:  309 LQKAIGAVPLIQGEYMIPCEKVSSLPTVYLKLGGKNYELHPDKYILKVSQGGKTICLSGFMGMDIPPPSGPLWILGDVFIGSYYTVFDRDNNRVGFA 405          
BLAST of EY665743 vs. ExPASy Swiss-Prot
Match: CATD_HUMAN (Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 6.154e-22
Identity = 42/100 (42.00%), Postives = 67/100 (67.00%), Query Frame = 3
Query:  258 LFKLCEVLPNPMGKSFINCDDIASMPYVSFTIGNRSFPLSPEQYIFKIEEGHSTICISGFIALDVPPPQGPLWVLGDMFLRAYHTVFDFGNLQIGFAETA 557
            L K    +P   G+  I C+ ++++P ++  +G + + LSPE Y  K+ +   T+C+SGF+ +D+PPP GPLW+LGD+F+  Y+TVFD  N ++GFAE A
Sbjct:  311 LQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAA 410          
BLAST of EY665743 vs. ExPASy Swiss-Prot
Match: CATD_CHIHA (Cathepsin D OS=Chionodraco hamatus GN=ctsd PE=1 SV=2)

HSP 1 Score: 103.99 bits (258), Expect = 1.050e-21
Identity = 44/98 (44.90%), Postives = 66/98 (67.35%), Query Frame = 3
Query:  258 LFKLCEVLPNPMGKSFINCDDIASMPYVSFTIGNRSFPLSPEQYIFKIEEGHSTICISGFIALDVPPPQGPLWVLGDMFLRAYHTVFDFGNLQIGFAE 551
            L K     P   G+  +NCD + S+P +SFT+G + + L+ EQYI K+ +   T+C+SGF+ LD+P P GPLW+LGD+F+  Y+TVFD    ++GFA+
Sbjct:  297 LQKAIGAFPLIQGEYMVNCDTVPSLPVISFTVGGQVYTLTGEQYILKVTQAGKTMCLSGFMGLDIPAPAGPLWILGDVFMGQYYTVFDRDANRVGFAK 394          
BLAST of EY665743 vs. ExPASy Swiss-Prot
Match: CATD_CHICK (Cathepsin D OS=Gallus gallus GN=CTSD PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.339e-21
Identity = 44/86 (51.16%), Postives = 62/86 (72.09%), Query Frame = 3
Query:  294 GKSFINCDDIASMPYVSFTIGNRSFPLSPEQYIFKIEEGHSTICISGFIALDVPPPQGPLWVLGDMFLRAYHTVFDFGNLQIGFAE 551
            G+  I+CD I+S+P V+  +G + + L+ EQY+FK+     TIC+SGF  LDVPPP GPLW+LGD+F+  Y+TVFD  N  +GFA+
Sbjct:  311 GQYVISCDKISSLPVVTLMLGGKPYQLTGEQYVFKVSAQGETICLSGFSGLDVPPPGGPLWILGDVFIGPYYTVFDRDNDSVGFAK 396          
The following BLAST results are available for this feature:
BLAST of EY665743 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 73
Match NameE-valueIdentityDescription
ASPRX_ORYSJ3.657e-4060.20Aspartic proteinase OS=Oryza sativa subsp. japonic... [more]
ASPR1_ORYSJ8.085e-4058.16Aspartic proteinase oryzasin-1 OS=Oryza sativa sub... [more]
ASPR_CUCPE6.711e-3958.16Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1[more]
ASPR_HORVU7.256e-3857.14Phytepsin OS=Hordeum vulgare PE=1 SV=1[more]
CYPR1_CYNCA1.606e-3760.20Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO... [more]
CATD_RAT4.712e-2243.00Cathepsin D OS=Rattus norvegicus GN=Ctsd PE=1 SV=1[more]
CATD_MOUSE4.712e-2244.33Cathepsin D OS=Mus musculus GN=Ctsd PE=1 SV=1[more]
CATD_HUMAN6.154e-2242.00Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1[more]
CATD_CHIHA1.050e-2144.90Cathepsin D OS=Chionodraco hamatus GN=ctsd PE=1 SV... [more]
CATD_CHICK2.339e-2151.16Cathepsin D OS=Gallus gallus GN=CTSD PE=1 SV=1[more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-102-095-B01-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY665743 ID=EY665743; Name=EY665743; organism=Citrus sinensis; type=EST; length=816bp
CTATATGAAGTAATTCCTCTTTTCTATCCTCTTTGGNTTTATCATTTGCA
ATCCTCTTGCTTAAACCTTATTTGAGATCCATGCAGCACTGGTATTGAGA
CTGTTGTGCAACATAAAACCAGCAACGGGTCATCTATAAATGAGAGCACG
CTATGCGCTTTCTGTGAGATGATTGTTTTCTGGATTCAAATGCAGCTTAA
ACAACAAAAAACAAAGGAGGCAATATTCAAATATGCAGATAAGTATTTCT
GTTGTGTTTGTTTAAGCTTTGCGAGGTACTTCCAAACCCCATGGGAAAAT
CATTTATCAACTGTGATGACATCGCAAGCATGCCATATGTTTCGTTCACC
ATTGGAAATAGGTCCTTCCCCCTCTCTCCAGAACAGTATATTTTCAAAAT
TGAAGAAGGCCATTCTACCATATGTATTAGTGGGTTCATCGCATTGGATG
TACCTCCGCCGCAAGGTCCCCTTTGGGTTCTAGGAGACATGTTCCTGAGG
GCATATCACACAGTTTTTGATTTTGGTAATCTACAAATAGGATTTGCTGA
AACTGCATAACTAGTATGGCTAGAATTCATCCCCCCGCTATCTGGAAATT
GGTTGATGCTACAACCAAATAATGCGATATCCCCAGGAATTAAAGCAAAA
AGGGAGGTGGTTAATTTGTTTAAATTTCCTGACTTTACCACCCACACTTT
GAATCTCCTTAAATGGAGCCTGGATCCCTTAAAAAAAAAAAAAAAAAAAA
ACAAACAAGAAAAAAGAATCCAACAACAGACAAGGGGGGGGCCCGTCCTA
AAAAAAACCAACTTTA
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