CX301559

Overview
NameCX301559
Unique NameCX301559
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
PhyRootSw1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX301559 vs. ExPASy Swiss-Prot
Match: CRK12_ARATH (Putative cysteine-rich receptor-like protein kinase 12 OS=Arabidopsis thaliana GN=CRK12 PE=2 SV=2)

HSP 1 Score: 215.312 bits (547), Expect = 3.202e-56
Identity = 107/175 (61.14%), Postives = 133/175 (76.00%), Query Frame = 3
Query:  147 QEKTENNRETDQVQNMDLELPLFELATIANATDNFSINNKLGEGGFGPVYKGTLVDGQEIAVKRLSKISEQGLKELKNEVILFSKLQHRNLVKVLGCCIQGEEKLLIYEFMPNKSLNSFIFDQERCKILDWSKRFHIICGTARGLLYLHQDSRLRIIHRDLKASNVLLDQDMNPK 671
            + K+    + DQ     L     +  TI  AT+NF+  NKLG+GGFG VYKGTLV+G E+AVKRLSK SEQG +E KNEV+L +KLQHRNLVK+LG C++ EEK+L+YEF+PNKSL+ F+FD  +   LDW+KR++II G  RG+LYLHQDSRL IIHRDLKASN+LLD DM PK
Sbjct:  333 RRKSYQEIDLDQSGITTLHFQQLDFKTIEVATENFAKTNKLGQGGFGEVYKGTLVNGTEVAVKRLSKTSEQGAQEFKNEVVLVAKLQHRNLVKLLGYCLEPEEKILVYEFVPNKSLDYFLFDPTKQGQLDWTKRYNIIGGITRGILYLHQDSRLTIIHRDLKASNILLDADMIPK 507          

HSP 2 Score: 25.0238 bits (53), Expect = 3.202e-56
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 2
Query:  665 PKISDFGLARAFGGDE 712
            PKI+DFG+AR  G D+
Sbjct:  506 PKIADFGMARISGIDQ 521          
BLAST of CX301559 vs. ExPASy Swiss-Prot
Match: SRK6_BRAOE (Putative serine/threonine-protein kinase receptor OS=Brassica oleracea var. acephala GN=SRK6 PE=2 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 5.434e-56
Identity = 103/159 (64.78%), Postives = 128/159 (80.50%), Query Frame = 3
Query:  195 DLELPLFELATIANATDNFSINNKLGEGGFGPVYKGTLVDGQEIAVKRLSKISEQGLKELKNEVILFSKLQHRNLVKVLGCCIQGEEKLLIYEFMPNKSLNSFIFDQERCKILDWSKRFHIICGTARGLLYLHQDSRLRIIHRDLKASNVLLDQDMNPK 671
            +LELPL E+ T+  AT+NFS  NKLG+GGFG VYKG L+DG+EIAVKRLSK S QG  E  NEV L ++LQH NLV+VLGCCI+G+EK+LIYE++ N SL+S++F + R   L+W++RF I  G ARGLLYLHQDSR RIIHRDLK SN+LLD++M PK
Sbjct:  510 ELELPLIEMETVVKATENFSSCNKLGQGGFGIVYKGRLLDGKEIAVKRLSKTSVQGTDEFMNEVTLIARLQHINLVQVLGCCIEGDEKMLIYEYLENLSLDSYLFGKTRRSKLNWNERFDITNGVARGLLYLHQDSRFRIIHRDLKVSNILLDKNMIPK 668          

HSP 2 Score: 28.1054 bits (61), Expect = 5.434e-56
Identity = 12/16 (75.00%), Postives = 13/16 (81.25%), Query Frame = 2
Query:  665 PKISDFGLARAFGGDE 712
            PKISDFG+AR F  DE
Sbjct:  667 PKISDFGMARIFERDE 682          
BLAST of CX301559 vs. ExPASy Swiss-Prot
Match: CRK18_ARATH (Cysteine-rich receptor-like protein kinase 18 OS=Arabidopsis thaliana GN=CRK18 PE=2 SV=2)

HSP 1 Score: 209.149 bits (531), Expect = 2.050e-55
Identity = 106/170 (62.35%), Postives = 130/170 (76.47%), Query Frame = 3
Query:  162 NNRETDQVQNMDLELPLFELATIANATDNFSINNKLGEGGFGPVYKGTLVDGQEIAVKRLSKISEQGLKELKNEVILFSKLQHRNLVKVLGCCIQGEEKLLIYEFMPNKSLNSFIFDQERCKILDWSKRFHIICGTARGLLYLHQDSRLRIIHRDLKASNVLLDQDMNPK 671
            N R+  Q  ++  E   F+L TI +AT NFS  NKLG+GGFG VYKG L++G EIAVKRLSK S QG  E KNEV++ +KLQH NLV++LG  +QGEEKLL+YEF+ NKSL+ F+FD  +   LDW+ R +II G  RG+LYLHQDSRL+IIHRDLKASN+LLD DMNPK
Sbjct:  310 NRRKQKQEMDLPTESVQFDLKTIESATSNFSERNKLGKGGFGEVYKGMLMNGTEIAVKRLSKTSGQGEVEFKNEVVVVAKLQHINLVRLLGFSLQGEEKLLVYEFVSNKSLDYFLFDPTKRNQLDWTMRRNIIGGITRGILYLHQDSRLKIIHRDLKASNILLDADMNPK 479          

HSP 2 Score: 28.4906 bits (62), Expect = 2.050e-55
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = 2
Query:  665 PKISDFGLARAFGGDE 712
            PKI+DFG+AR FG D+
Sbjct:  478 PKIADFGMARIFGVDQ 493          
BLAST of CX301559 vs. ExPASy Swiss-Prot
Match: CRK25_ARATH (Cysteine-rich receptor-like protein kinase 25 OS=Arabidopsis thaliana GN=CRK25 PE=2 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 3.484e-55
Identity = 100/179 (55.87%), Postives = 135/179 (75.42%), Query Frame = 3
Query:  153 KTENNRETDQVQNMD------LELPLFELATIANATDNFSINNKLGEGGFGPVYKGTLVDGQEIAVKRLSKISEQGLKELKNEVILFSKLQHRNLVKVLGCCIQGEEKLLIYEFMPNKSLNSFIFDQERCKILDWSKRFHIICGTARGLLYLHQDSRLRIIHRDLKASNVLLDQDMNPK 671
            +  NN+ + + +++D       E   F+ + I  AT+ FS +NKLG GGFG VYKG L+ G+ +A+KRLS+ S QG +E KNEV + +KLQHRNL K+LG C+ GEEK+L+YEF+PNKSL+ F+FD E+ ++LDW +R+ II G ARG+LYLH+DSRL IIHRDLKASN+LLD DM+PK
Sbjct:  309 RRRNNKLSAETEDLDEDGITSTETLQFQFSAIEAATNKFSESNKLGHGGFGEVYKGQLITGETVAIKRLSQGSTQGAEEFKNEVDVVAKLQHRNLAKLLGYCLDGEEKILVYEFVPNKSLDYFLFDNEKRRVLDWQRRYKIIEGIARGILYLHRDSRLTIIHRDLKASNILLDADMHPK 487          

HSP 2 Score: 29.6462 bits (65), Expect = 3.484e-55
Identity = 12/16 (75.00%), Postives = 14/16 (87.50%), Query Frame = 2
Query:  665 PKISDFGLARAFGGDE 712
            PKISDFG+AR FG D+
Sbjct:  486 PKISDFGMARIFGVDQ 501          
BLAST of CX301559 vs. ExPASy Swiss-Prot
Match: CRK10_ARATH (Cysteine-rich receptor-like protein kinase 10 OS=Arabidopsis thaliana GN=CRK10 PE=1 SV=2)

HSP 1 Score: 208.379 bits (529), Expect = 4.543e-55
Identity = 100/149 (67.11%), Postives = 123/149 (82.55%), Query Frame = 3
Query:  225 TIANATDNFSINNKLGEGGFGPVYKGTLVDGQEIAVKRLSKISEQGLKELKNEVILFSKLQHRNLVKVLGCCIQGEEKLLIYEFMPNKSLNSFIFDQERCKILDWSKRFHIICGTARGLLYLHQDSRLRIIHRDLKASNVLLDQDMNPK 671
            TI  ATD+F  +NK+G+GGFG VYKGTL DG E+AVKRLSK S QG  E KNEV+L +KLQHRNLV++LG C+ GEE++L+YE++PNKSL+ F+FD  +   LDW++R+ II G ARG+LYLHQDSRL IIHRDLKASN+LLD DMNPK
Sbjct:  329 TIQTATDDFVESNKIGQGGFGEVYKGTLSDGTEVAVKRLSKSSGQGEVEFKNEVVLVAKLQHRNLVRLLGFCLDGEERVLVYEYVPNKSLDYFLFDPAKKGQLDWTRRYKIIGGVARGILYLHQDSRLTIIHRDLKASNILLDADMNPK 477          

HSP 2 Score: 28.1054 bits (61), Expect = 4.543e-55
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = 2
Query:  665 PKISDFGLARAFGGDE 712
            PKI+DFG+AR FG D+
Sbjct:  476 PKIADFGMARIFGLDQ 491          
BLAST of CX301559 vs. ExPASy Swiss-Prot
Match: CRK17_ARATH (Cysteine-rich receptor-like protein kinase 17 OS=Arabidopsis thaliana GN=CRK17 PE=2 SV=2)

HSP 1 Score: 207.608 bits (527), Expect = 5.920e-55
Identity = 107/171 (62.57%), Postives = 130/171 (76.02%), Query Frame = 3
Query:  186 QNMDLELPL---------FELATIANATDNFSINNKLGEGGFGPVYKGTLVDGQEIAVKRLSKISEQGLKELKNEVILFSKLQHRNLVKVLGCCIQGEEKLLIYEFMPNKSLNSFIFDQERCKILDWSKRFHIICGTARGLLYLHQDSRLRIIHRDLKASNVLLDQDMNPK 671
            Q  ++ELP+         F+L TI  AT NFS +NKLG GGFG VYKG L++G EIAVKRLSK S QG  E KNEV++ +KLQH NLV++LG  +QGEEKLL+YEF+PNKSL+ F+FD  +   LDW+ R +II G  RG+LYLHQDSRL+IIHRDLKASN+LLD DMNPK
Sbjct:  329 QKQEIELPIVLNEAESVQFDLKTIEAATGNFSEHNKLGAGGFGEVYKGMLLNGTEIAVKRLSKTSGQGEIEFKNEVVVVAKLQHINLVRLLGFSLQGEEKLLVYEFVPNKSLDYFLFDPNKRNQLDWTVRRNIIGGITRGILYLHQDSRLKIIHRDLKASNILLDADMNPK 499          

HSP 2 Score: 28.4906 bits (62), Expect = 5.920e-55
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = 2
Query:  665 PKISDFGLARAFGGDE 712
            PKI+DFG+AR FG D+
Sbjct:  498 PKIADFGMARIFGVDQ 513          
BLAST of CX301559 vs. ExPASy Swiss-Prot
Match: CRK35_ARATH (Putative cysteine-rich receptor-like protein kinase 35 OS=Arabidopsis thaliana GN=CRK35 PE=3 SV=2)

HSP 1 Score: 207.223 bits (526), Expect = 7.719e-55
Identity = 99/153 (64.71%), Postives = 122/153 (79.74%), Query Frame = 3
Query:  213 FELATIANATDNFSINNKLGEGGFGPVYKGTLVDGQEIAVKRLSKISEQGLKELKNEVILFSKLQHRNLVKVLGCCIQGEEKLLIYEFMPNKSLNSFIFDQERCKILDWSKRFHIICGTARGLLYLHQDSRLRIIHRDLKASNVLLDQDMNPK 671
            F   TI  ATD FS +N +G GGFG VY+G L  G E+AVKRLSK S QG +E KNE +L SKLQH+NLV++LG C++GEEK+L+YEF+PNKSL+ F+FD  +   LDW++R++II G ARG+LYLHQDSRL IIHRDLKASN+LLD DMNPK
Sbjct:  333 FSFKTIEAATDKFSDSNMIGRGGFGEVYRGKLSSGPEVAVKRLSKTSGQGAEEFKNEAVLVSKLQHKNLVRLLGFCLEGEEKILVYEFVPNKSLDYFLFDPAKQGELDWTRRYNIIGGIARGILYLHQDSRLTIIHRDLKASNILLDADMNPK 485          

HSP 2 Score: 28.4906 bits (62), Expect = 7.719e-55
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = 2
Query:  665 PKISDFGLARAFGGDE 712
            PKI+DFG+AR FG D+
Sbjct:  484 PKIADFGMARIFGVDQ 499          
BLAST of CX301559 vs. ExPASy Swiss-Prot
Match: CRK29_ARATH (Cysteine-rich receptor-like protein kinase 29 OS=Arabidopsis thaliana GN=CRK29 PE=2 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 3.788e-54
Identity = 106/176 (60.23%), Postives = 130/176 (73.86%), Query Frame = 3
Query:  144 GQEKTENNRETDQVQNMDLELPLFELATIANATDNFSINNKLGEGGFGPVYKGTLVDGQEIAVKRLSKISEQGLKELKNEVILFSKLQHRNLVKVLGCCIQGEEKLLIYEFMPNKSLNSFIFDQERCKILDWSKRFHIICGTARGLLYLHQDSRLRIIHRDLKASNVLLDQDMNPK 671
            G+     +   D+  N +  L  FE  T+  ATDNFS  N+LG GGFG VYKG    GQEIAVKRLS  S QG  E KNE++L +KLQHRNLV+++G CIQGEE+LL+YEF+ N SL+ FIFD E+ ++LDW  R+ +I G ARGLLYLH+DSR RIIHRDLKASN+LLDQ+MNPK
Sbjct:  324 GKSPLSGSIAEDEFSNTESLLVHFE--TLKTATDNFSSENELGRGGFGSVYKGVFPQGQEIAVKRLSGNSGQGDNEFKNEILLLAKLQHRNLVRLIGFCIQGEERLLVYEFIKNASLDQFIFDTEKRQLLDWVVRYKMIGGIARGLLYLHEDSRFRIIHRDLKASNILLDQEMNPK 497          

HSP 2 Score: 23.8682 bits (50), Expect = 3.788e-54
Identity = 9/12 (75.00%), Postives = 11/12 (91.67%), Query Frame = 2
Query:  665 PKISDFGLARAF 700
            PKI+DFGLA+ F
Sbjct:  496 PKIADFGLAKLF 507          
BLAST of CX301559 vs. ExPASy Swiss-Prot
Match: CRK5_ARATH (Cysteine-rich receptor-like protein kinase 5 OS=Arabidopsis thaliana GN=CRK5 PE=1 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 3.789e-54
Identity = 106/185 (57.30%), Postives = 135/185 (72.97%), Query Frame = 3
Query:  120 ISAYHLDQGQEKTENNRETDQVQNM-DLELPLFELATIANATDNFSINNKLGEGGFGPVYKGTLVDGQEIAVKRLSKISEQGLKELKNEVILFSKLQHRNLVKVLGCCIQGEEKLLIYEFMPNKSLNSFIFDQERCKILDWSKRFHIICGTARGLLYLHQDSRLRIIHRDLKASNVLLDQDMNPK 671
            + ++H  +  +KT +  E D +     L+   F+   I  ATD FS+ NKLG+GGFG VYKGTL +G ++AVKRLSK S QG KE KNEV++ +KLQHRNLVK+LG C++ EEK+L+YEF+ NKSL+ F+FD      LDW+ R+ II G ARG+LYLHQDSRL IIHRDLKA N+LLD DMNPK
Sbjct:  299 VFSFHASKRAKKTYDTPEEDDITTAGSLQ---FDFKVIEAATDKFSMCNKLGQGGFGQVYKGTLPNGVQVAVKRLSKTSGQGEKEFKNEVVVVAKLQHRNLVKLLGFCLEREEKILVYEFVSNKSLDYFLFDSRMQSQLDWTTRYKIIGGIARGILYLHQDSRLTIIHRDLKAGNILLDADMNPK 480          

HSP 2 Score: 24.6386 bits (52), Expect = 3.789e-54
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 2
Query:  665 PKISDFGLARAFGGDE 712
            PK++DFG+AR F  D+
Sbjct:  479 PKVADFGMARIFEIDQ 494          
BLAST of CX301559 vs. ExPASy Swiss-Prot
Match: CRK37_ARATH (Cysteine-rich receptor-like protein kinase 37 OS=Arabidopsis thaliana GN=CRK37 PE=2 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 4.940e-54
Identity = 100/153 (65.36%), Postives = 124/153 (81.05%), Query Frame = 3
Query:  213 FELATIANATDNFSINNKLGEGGFGPVYKGTLVDGQEIAVKRLSKISEQGLKELKNEVILFSKLQHRNLVKVLGCCIQGEEKLLIYEFMPNKSLNSFIFDQERCKILDWSKRFHIICGTARGLLYLHQDSRLRIIHRDLKASNVLLDQDMNPK 671
            F+L  I  AT+NFS+ NKLG+GGFG VYKG L  GQEIAVKRL K S QG  E KNEV+L ++LQHRNLVK+LG C + +E++L+YEF+PN SL+ FIFD+E+ ++L W  R+ II G ARGLLYLH+DS+LRIIHRDLKASN+LLD +MNPK
Sbjct:  333 FDLRMIVTATNNFSLENKLGQGGFGSVYKGILPSGQEIAVKRLRKGSGQGGMEFKNEVLLLTRLQHRNLVKLLGFCNEKDEEILVYEFVPNSSLDHFIFDEEKRRVLTWDVRYTIIEGVARGLLYLHEDSQLRIIHRDLKASNILLDAEMNPK 485          

HSP 2 Score: 26.5646 bits (57), Expect = 4.940e-54
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 2
Query:  665 PKISDFGLARAFGGDE 712
            PK++DFG+AR F  DE
Sbjct:  484 PKVADFGMARLFDMDE 499          
The following BLAST results are available for this feature:
BLAST of CX301559 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 336
Match NameE-valueIdentityDescription
CRK12_ARATH3.202e-5661.14Putative cysteine-rich receptor-like protein kinas... [more]
SRK6_BRAOE5.434e-5664.78Putative serine/threonine-protein kinase receptor ... [more]
CRK18_ARATH2.050e-5562.35Cysteine-rich receptor-like protein kinase 18 OS=A... [more]
CRK25_ARATH3.484e-5555.87Cysteine-rich receptor-like protein kinase 25 OS=A... [more]
CRK10_ARATH4.543e-5567.11Cysteine-rich receptor-like protein kinase 10 OS=A... [more]
CRK17_ARATH5.920e-5562.57Cysteine-rich receptor-like protein kinase 17 OS=A... [more]
CRK35_ARATH7.719e-5564.71Putative cysteine-rich receptor-like protein kinas... [more]
CRK29_ARATH3.788e-5460.23Cysteine-rich receptor-like protein kinase 29 OS=A... [more]
CRK5_ARATH3.789e-5457.30Cysteine-rich receptor-like protein kinase 5 OS=Ar... [more]
CRK37_ARATH4.940e-5465.36Cysteine-rich receptor-like protein kinase 37 OS=A... [more]

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Properties
Property NameValue
Genbank descriptionC08011B01SK PhyRootSw1 Citrus sinensis cDNA clone C08011B01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX301559 ID=CX301559; Name=CX301559; organism=Citrus sinensis; type=EST; length=712bp
AATTCGGCACGAGGCGATTTGATGCAGGCAAATCAAACTCTTATAAATAT
TAAATTTATAAGAGTAGATTAAATTTTATATGCAGCAATAACTGTATATT
TGTTTCAAGATGAAGAGTTATCAGTGCATATCATTTAGATCAGGGGCAAG
AGAAAACAGAAAACAACAGGGAAACTGATCAGGTTCAAAACATGGACCTG
GAGCTACCACTGTTTGAGTTAGCTACAATTGCTAATGCCACCGATAACTT
TTCAATCAACAACAAGCTTGGAGAAGGTGGCTTTGGACCTGTTTACAAGG
GCACGCTAGTGGATGGACAAGAAATTGCTGTGAAGAGGCTTTCGAAGATT
TCTGAGCAAGGATTGAAGGAGTTAAAGAATGAAGTTATACTATTTTCCAA
ATTACAGCACAGAAATCTTGTAAAGGTTCTTGGCTGCTGCATTCAAGGAG
AGGAGAAATTGTTGATTTATGAATTCATGCCCAACAAAAGCCTGAACTCT
TTCATTTTTGATCAAGAAAGATGTAAGATATTGGATTGGTCCAAGCGTTT
CCACATTATTTGTGGGACTGCTAGGGGCCTTCTCTACCTTCATCAAGATT
CTAGATTGAGGATTATACATCGAGATCTCAAAGCAAGTAATGTGCTACTC
GATCAAGATATGAACCCAAAATTTCAGATTTTGGTTTGGCAAGAGCTTTT
GGTGGAGACGAA
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