DY257267

Overview
NameDY257267
Unique NameDY257267
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1259
Libraries
Library NameType
AbsCFruitcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DY257267 vs. ExPASy Swiss-Prot
Match: NAT2_ARATH (Nucleobase-ascorbate transporter 2 OS=Arabidopsis thaliana GN=NAT2 PE=2 SV=2)

HSP 1 Score: 328.176 bits (840), Expect = 6.421e-89
Identity = 167/236 (70.76%), Postives = 189/236 (80.08%), Query Frame = -1
Query:  204 LSQLEQQGGF----RFSKCHPPSCPGFLPGFGWQGMG---SYRVDSLDH*AVFLSR*KMWGSWKHSCGSRRVIQISAGFMIFFSMLGKFGAFFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSVPEYFREYTAKALHGPAHTRAGWFNDFLNTIFFSSPTVALIVAVFLDNTLDYKDSAKDRGMPWWVKFRTFKGDTRNEEFYTLPFNLNRFFPPS 890
            +S +E  G F    R +   PP       G GWQG+G   +    +L   +V +    + GS +   GSRRVIQISAGFMIFFSMLGKFGA FASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNS+RNLFI GV+LFLGLS+PEYFR+++ KALHGPAHT AGWFNDFLNTIF SSP VAL+VAVFLDNTLDYK++A+DRG+PWW KFRTFKGD+RNEEFYTLPFNLNRFFPPS
Sbjct:  291 VSLIESTGAFKAAARLASATPPPPHVLSRGIGWQGIGILLNGLFGTLSGSSVSVENIGLLGSTR--VGSRRVIQISAGFMIFFSMLGKFGALFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSLRNLFIVGVSLFLGLSIPEYFRDFSMKALHGPAHTNAGWFNDFLNTIFLSSPMVALMVAVFLDNTLDYKETARDRGLPWWAKFRTFKGDSRNEEFYTLPFNLNRFFPPS 524          
BLAST of DY257267 vs. ExPASy Swiss-Prot
Match: NAT1_ARATH (Nucleobase-ascorbate transporter 1 OS=Arabidopsis thaliana GN=NAT1 PE=2 SV=1)

HSP 1 Score: 270.011 bits (689), Expect = 2.075e-71
Identity = 149/276 (53.99%), Postives = 181/276 (65.58%), Query Frame = -1
Query:  204 LIFLFPGLKFPIFFSGGVPTL*WWVLLLGDGCPFWFH*L-SQLEQQGGF----RFSKCHPPSCPGFLPGFGWQGMGSYRVDSL----DH*AVFLSR*KMWGSWKHSCGSRRVIQISAGFMIFFSMLGKFGAFFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSVPEYFREYTAKALHGPAHTRAGWFNDFLNTIFFSSPTVALIVAVFLDNTLDYKDSAKDRGMPWWVKFRTFKGDTRNEEFYTLPFNLNRFFPPS 1004
            LI   P  KFP     G PT       +G         L S +E  G +    R +   PP       G GWQG+G   +D L        V +    + G  +   GSRRV+Q+SAGFMI FS LGKFGA FASIP  I+AA++C+LFGLVA+VGLSFLQFTNMNSMRNL ITG++LFLG+S+P++F +Y     +G  HT AGWFN FLNT+F S  TV LI+AVF+DNT++ + S KDRGMPWWVKFRTF+GD RNEEFYTLPFNLNRFFPP+
Sbjct:  253 LISTAPWFKFPYPLQWGPPTF-----SVGHSFAMMSAVLVSMVESTGAYIAASRLAIATPPPAYVLSRGIGWQGIGVL-LDGLFGTGTGSTVLVENVGLLGLTR--VGSRRVVQVSAGFMIVFSTLGKFGAVFASIPVPIYAALHCILFGLVAAVGLSFLQFTNMNSMRNLMITGLSLFLGISIPQFFAQYWDARHYGLVHTNAGWFNAFLNTLFMSPATVGLIIAVFMDNTMEVERSKKDRGMPWWVKFRTFRGDNRNEEFYTLPFNLNRFFPPT 520          
BLAST of DY257267 vs. ExPASy Swiss-Prot
Match: NAT6_ARATH (Nucleobase-ascorbate transporter 6 OS=Arabidopsis thaliana GN=NAT6 PE=2 SV=2)

HSP 1 Score: 227.639 bits (579), Expect = 4.645e-61
Identity = 112/169 (66.27%), Postives = 131/169 (77.51%), Query Frame = -1
Query:  210 GSRRVIQISAGFMIFFSMLGKFGAFFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSVPEYFREYTAKALHGPAHTRAGWFNDFLNTIFFSSPTVALIVAVFLDNTLDYKDSA--KDRGMPWWVKFRTFKGDTRNEEFYTLPFNLNRFFP 710
            GSRRV+QI+AGFMIFFS+LGKFGA FASIP  I AA+YC+ F  V + GLSFLQF N+NS R  FI G ++FLGLS+P+YF EYTA   +GP HT A WFND +N  F S P VA  VA FLDNTL  KDS+  KDRG  WW KFR+FKGDTR+EEFY+LPFNLN++FP
Sbjct:  362 GSRRVVQIAAGFMIFFSILGKFGAVFASIPAPIIAALYCLFFAYVGAGGLSFLQFCNLNSFRTKFILGFSVFLGLSIPQYFNEYTAIKGYGPVHTGARWFNDMVNVPFSSEPFVAGSVAFFLDNTLHKKDSSIRKDRGKHWWDKFRSFKGDTRSEEFYSLPFNLNKYFP 530          

HSP 2 Score: 26.9498 bits (58), Expect = 4.645e-61
Identity = 19/57 (33.33%), Postives = 29/57 (50.88%), Query Frame = -3
Query:  826 IKIPNFLFRGGSYPLMVGPAFG*WVPVLVPLIESTGA----TRGLPVFQMPPPLLPR 984
            I++P + F+ G+     G AF   +   V L+ESTGA    +R      +PP +L R
Sbjct:  270 IRVP-WPFQWGAPSFDAGEAFAMMMASFVALVESTGAFVAVSRYASATMLPPSILSR 325          
BLAST of DY257267 vs. ExPASy Swiss-Prot
Match: NAT7_ARATH (Nucleobase-ascorbate transporter 7 OS=Arabidopsis thaliana GN=NAT7 PE=2 SV=2)

HSP 1 Score: 218.779 bits (556), Expect = 5.486e-56
Identity = 121/246 (49.19%), Postives = 154/246 (62.60%), Query Frame = -1
Query:  210 LSQLEQQGGF----RFSKCHPPSCPGFLPGFGWQGMG-------------SYRVDSLDH*AVFLSR*KMWGSWKHSCGSRRVIQISAGFMIFFSMLGKFGAFFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSVPEYFREYTAKALHGPAHTRAGWFNDFLNTIFFSSPTVALIVAVFLDNTLDYKDSA--KDRGMPWWVKFRTFKGDTRNEEFYTLPFNLNRFFP 890
            +S +E  G +    RF+   PP       G GWQG+G             S  V++     + L+R           GSRRV+QISAGFMIFFS+LGKFGA FASIP  + AA++C+ F  V + GLS LQF N+NS R  FI G ++F+GLS+P+YF +YTA   +GP HT A WFND +N  F S   VA I+A FLD T+  KDSA  KDRGM WW +F +FK DTR+EEFY+LPFNLN++FP
Sbjct:  303 VSLIESTGTYIVVSRFASATPPPPSVLSRGVGWQGVGVLLCGLFGAGNGASVSVENAG--LLALTR----------VGSRRVVQISAGFMIFFSILGKFGAIFASIPAPVVAALHCLFFAYVGAGGLSLLQFCNLNSFRTKFILGFSVFMGLSIPQYFNQYTAVNKYGPVHTHARWFNDMINVPFSSKAFVAGILAFFLDVTMSSKDSATRKDRGMFWWDRFMSFKSDTRSEEFYSLPFNLNKYFP 536          
BLAST of DY257267 vs. ExPASy Swiss-Prot
Match: NAT3_ARATH (Nucleobase-ascorbate transporter 3 OS=Arabidopsis thaliana GN=NAT3 PE=2 SV=2)

HSP 1 Score: 211.075 bits (536), Expect = 1.144e-53
Identity = 97/168 (57.74%), Postives = 127/168 (75.60%), Query Frame = -1
Query:  210 GSRRVIQISAGFMIFFSMLGKFGAFFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSVPEYFREYTAKALHGPAHTRAGWFNDFLNTIFFSSPTVALIVAVFLDNTLDYKDSAKD-RGMPWWVKFRTFKGDTRNEEFYTLPFNLNRFFP 710
            GSRRV+Q+S  FMIFFS+ GKFGAFFASIP  IFA VYC+L G+V +VG+SF+QFT+ NSMRN+++ GV+LFL LS+ +YF   T++A +GP  T  GWFND LNTIF S+P VA I+A  LDNTL+ + ++ D RG+PWW  F+   GD RN+EFY++P  +N   P
Sbjct:  380 GSRRVVQVSTFFMIFFSIFGKFGAFFASIPLPIFAGVYCILLGIVVAVGISFIQFTDTNSMRNMYVIGVSLFLSLSIAQYFLANTSRAGYGPVRTAGGWFNDILNTIFASAPLVATILATILDNTLEARHASDDARGIPWWKPFQHRNGDGRNDEFYSMPLRINELMP 547          
BLAST of DY257267 vs. ExPASy Swiss-Prot
Match: NAT5_ARATH (Nucleobase-ascorbate transporter 5 OS=Arabidopsis thaliana GN=NAT5 PE=2 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 5.678e-53
Identity = 104/169 (61.54%), Postives = 121/169 (71.60%), Query Frame = -1
Query:  210 GSRRVIQISAGFMIFFSMLGKFGAFFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSVPEYFREYTAKALHGPAHTRAGWFNDFLNTIFFSSPTVALIVAVFLDNTLDYKDSA--KDRGMPWWVKFRTFKGDTRNEEFYTLPFNLNRFFP 710
            GSRRV+QISAGFMIFFS+LGKFGA FASIP  I AA+YC+ F  V + GLS LQF N+NS R LFI G ++FLGLS+P+YF E+TA   +GP HT A WFND +N  F S   V   VA  LD TL  KD +  KDRG  WW +F TFK D R EEFY LPFNLN++FP
Sbjct:  358 GSRRVVQISAGFMIFFSILGKFGAVFASIPSPIIAALYCLFFAYVGAGGLSLLQFCNLNSFRTLFILGFSIFLGLSIPQYFNEHTAIKGYGPVHTGARWFNDMVNVPFSSKAFVGGCVAYLLDTTLHKKDGSIRKDRGKHWWDRFWTFKNDPRTEEFYALPFNLNKYFP 526          
BLAST of DY257267 vs. ExPASy Swiss-Prot
Match: NAT8_ARATH (Nucleobase-ascorbate transporter 8 OS=Arabidopsis thaliana GN=NAT8 PE=2 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 9.122e-53
Identity = 94/169 (55.62%), Postives = 124/169 (73.37%), Query Frame = -1
Query:  210 GSRRVIQISAGFMIFFSMLGKFGAFFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSVPEYFREYTAKALHGPAHTRAGWFNDFLNTIFFSSPTVALIVAVFLDNTLDYKD--SAKDRGMPWWVKFRTFKGDTRNEEFYTLPFNLNRFFP 710
            GSRRVIQ++AGFMIFFS+LGKFGA FASIP  I AA+YC+ F  V + GLS +QF N+NS R  FI G ++F+GLS+P+YF +YT    +GP  T A WFN+ +N  F S   V+ I+A FLD TL  KD  + KDRG+ WW +F++F+ D R+EEFY+LP NL+++FP
Sbjct:  369 GSRRVIQVAAGFMIFFSILGKFGAIFASIPAPIVAALYCLFFSYVGAGGLSLIQFCNLNSFRTKFILGFSIFMGLSIPQYFYQYTTLETYGPVRTSATWFNNIINVPFSSKAFVSGILAFFLDTTLPPKDKTTKKDRGLVWWKRFKSFQSDNRSEEFYSLPLNLSKYFP 537          

HSP 2 Score: 27.335 bits (59), Expect = 9.122e-53
Identity = 26/91 (28.57%), Postives = 35/91 (38.46%), Query Frame = -3
Query:  724 IKIPNFLFRGGSYPLMVGPAFG*WVPVLVPLIESTG----ATRGLPVFQMPPPLLPRFFAGFWLAGNGIXXXXXXXXXXXXXXXVENVGFL 984
            I++P+ +  GG+     G  F       V L+ESTG     +R      +PP +L R   G    G GI               VEN G L
Sbjct:  276 IRVPHPIQWGGAPTFNAGDIFAMMAASFVSLVESTGTYIAVSRYASATPIPPSVLSR---GIGWQGFGILLCGLFGAGNATSVSVENAGLL 363          
BLAST of DY257267 vs. ExPASy Swiss-Prot
Match: NAT4_ARATH (Nucleobase-ascorbate transporter 4 OS=Arabidopsis thaliana GN=NAT4 PE=2 SV=2)

HSP 1 Score: 199.134 bits (505), Expect = 4.499e-50
Identity = 107/207 (51.69%), Postives = 130/207 (62.80%), Query Frame = -1
Query:  210 GFGWQGMG---SYRVDSLDH*AVFLSR*KMWGSWKHSCGSRRVIQISAGFMIFFSMLGKFGAFFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSVPEYFREYTAKALHGPAHTRAGWFNDFLNTIFFSSPTVALIVAVFLDNTLDYKDSA--KDRGMPWWVKFRTFKGDTRNEEFYTLPFNLNRFFP 815
            G GWQG+G   +    +       +    + G  K   GSRRV+QISAGFMIFFS+ GKFGA  ASIP  IFAA+YCVLF  VAS GL  LQF N+NS RN FI G ++F+GLSV +YF EY   +  GP HTR   FN  +  IF S+ TV ++ A  LD T  Y  ++  +D G  WW KFR +  DTR EEFY LP+NLNRFFP
Sbjct:  320 GIGWQGIGVLLNGLFGTATGSTALVENTGLLGLTK--VGSRRVVQISAGFMIFFSIFGKFGAVLASIPLPIFAALYCVLFAYVASAGLGLLQFCNLNSFRNKFILGFSIFIGLSVAQYFTEYLFISGRGPVHTRTSAFNVIMQVIFSSAATVGIMAAFLLDCTHSYGHASVRRDSGRHWWEKFRVYHTDTRTEEFYALPYNLNRFFP 524          
BLAST of DY257267 vs. ExPASy Swiss-Prot
Match: NAT10_ARATH (Putative nucleobase-ascorbate transporter 10 OS=Arabidopsis thaliana GN=NAT10 PE=3 SV=2)

HSP 1 Score: 183.341 bits (464), Expect = 2.554e-45
Identity = 92/169 (54.44%), Postives = 114/169 (67.46%), Query Frame = -1
Query:  210 GSRRVIQISAGFMIFFSMLGKFGAFFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSVPEYFREYTAKALHGPAHTRAGWFNDFLNTIFFSSPTVALIVAVFLDNTL--DYKDSAKDRGMPWWVKFRTFKGDTRNEEFYTLPFNLNRFFP 710
            GSRRVIQISA FMIFFS+ GKFGAFFASIP  I A++YC++   V+SVGLS+LQF N+NS    FI G + F+ +S+P+YFREY         H  + W  D +  IF S  TVA I+A+ LD TL  D  ++ KD GM WW KFR +  D RN+EFY LP  LN+FFP
Sbjct:  373 GSRRVIQISAAFMIFFSIFGKFGAFFASIPLPIMASLYCIVLCFVSSVGLSYLQFCNLNSFNIKFILGFSFFMAISIPQYFREYYNGGWRSDHH--SNWLEDMIRVIFMSHTTVAAIIAIVLDCTLCRDSDEAKKDCGMKWWDKFRLYNLDVRNDEFYGLPCRLNKFFP 539          
BLAST of DY257267 vs. ExPASy Swiss-Prot
Match: LPE1_MAIZE (Nucleobase-ascorbate transporter LPE1 OS=Zea mays GN=LPE1 PE=1 SV=2)

HSP 1 Score: 182.185 bits (461), Expect = 5.691e-45
Identity = 94/169 (55.62%), Postives = 117/169 (69.23%), Query Frame = -1
Query:  210 GSRRVIQISAGFMIFFSMLGKFGAFFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSMRNLFITGVALFLGLSVPEYFREYTAKALHGPAHTRAGWFNDFLNTIFFSSPTVALIVAVFLDNTLDYKDSA--KDRGMPWWVKFRTFKGDTRNEEFYTLPFNLNRFFP 710
            GSRRVI+ISA FMIFFS+  KFGA  ASIP  IFAA+YCVLF   A  G S LQ+ N+NS+R  FI  ++LFLGLS+P+YFR Y      GP HT +  FN  +N IF S  TVA I+A  LD T  Y +++  KDRG  WW KF+++K D R+EEFY LP+ L+R+FP
Sbjct:  357 GSRRVIKISALFMIFFSLFAKFGAVLASIPLPIFAALYCVLFAYSAGAGFSLLQYCNLNSLRTKFILSISLFLGLSIPQYFRVYEMFFGFGPVHTHSVAFNVMVNVIFSSPATVAAILAYLLDCTHLYWEASVKKDRGWFWWEKFKSYKYDGRSEEFYRLPYGLSRYFP 525          
The following BLAST results are available for this feature:
BLAST of DY257267 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 20
Match NameE-valueIdentityDescription
NAT2_ARATH6.421e-8970.76Nucleobase-ascorbate transporter 2 OS=Arabidopsis ... [more]
NAT1_ARATH2.075e-7153.99Nucleobase-ascorbate transporter 1 OS=Arabidopsis ... [more]
NAT6_ARATH4.645e-6166.27Nucleobase-ascorbate transporter 6 OS=Arabidopsis ... [more]
NAT7_ARATH5.486e-5649.19Nucleobase-ascorbate transporter 7 OS=Arabidopsis ... [more]
NAT3_ARATH1.144e-5357.74Nucleobase-ascorbate transporter 3 OS=Arabidopsis ... [more]
NAT5_ARATH5.678e-5361.54Nucleobase-ascorbate transporter 5 OS=Arabidopsis ... [more]
NAT8_ARATH9.122e-5355.62Nucleobase-ascorbate transporter 8 OS=Arabidopsis ... [more]
NAT4_ARATH4.499e-5051.69Nucleobase-ascorbate transporter 4 OS=Arabidopsis ... [more]
NAT10_ARATH2.554e-4554.44Putative nucleobase-ascorbate transporter 10 OS=Ar... [more]
LPE1_MAIZE5.691e-4555.62Nucleobase-ascorbate transporter LPE1 OS=Zea mays ... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionKN0AAK1DB06FM2 AbsCFruit Citrus sinensis cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY257267 ID=DY257267; Name=DY257267; organism=Citrus sinensis; type=EST; length=1259bp
GAACAAAGAATAAATTATTGCATTAAACCCAAATAATTCGAGTTAAATTT
ACATATGAGATGAAAATAGGGAGAGAAACCAGAGTCATCATTTCTCTGCC
ATGCAAAACATATCCATCTCTGTCTCAAGTCTTCTCTGCAGTAGCTCGGA
GTTATCATTTCTTTTCTAGAAATGACAGCCTCTTTTAATATGACGCTACT
TCATGATGGAGGGAAGAAACGGTTGAGGTTGAAAGGGAGGGTGTAGAATT
CCTCATTCCGGGTGTCTCCTTTAAATGTGCGGAATTTAACCCACCATGGC
ATGCCTCTGTCTTTGGCACTGTCCTTGTAATCGAGTGTGTTGTCCAAGAA
AACAGCGACAATCAACGCAACAGTTGGAGATGAGAAGAATATGGTGTTCA
GGAAGTCATTGAACCATCCAGCCCTGGTGTGAGCAGGACCATGAAGAGCC
TTGGCAGTGTATTCTCTGAAGTATTCAGGAACCGACAAACCTAAGAAGAG
GGCTACACCGGTGATGAAGAGGTTCCTCATTGAGTTCATGTTTGTGAATT
GCAAAAATGATAGCCCCACAGAAGCAACGAGACCAAACAGGACACAGTAT
ACAGCAGCAAAAATGGTGAAGGGGATTGATGCAAAAAAAGCACCAAATTT
TCCTAGCATTGAGAAAAATATCATAAATCCAGCCGAGATTTGAATGACCC
TCCGGCTCCCACACGAGTGCTTCCAAGAACCCCACATTTTCTACCGAGAC
AAAAAAACCGCTCAATGGTCCAAAGAATCCACTCGGTAGGATCCCATTCC
CTGCCAGCCAAAACCCGGCAAAAAACCGGGGCAGGAGGGGGGGTGGCATT
TGGAAAACCGGAAGCCCCCTTGTTGCTCCAGTTGACTCAATCAATGGAAC
CAGAACGGGCACCCATCACCCAAAAGCAGGACCCACCATCAAAGGGTAGG
AACCCCCCCTGAAAAGAAAATTGGGAATTTTAATCCTGGAAACAAAAAAA
TTAAAGTTGGGCCTTGTCAGTTCCGGCCAATCTTTTTGGGTAAAACTCGA
GAAAATTTTTAAAAACGCCCCATCGGCGGGTCACGAAGCGGGGGGATTTC
CCCCCCAACCCCCGGTGTAAGGGTTTTTGAGAAGGAAAGGGAAACACCCC
CCCTCTTATTTCGGGGAAAAGGTGCCTTGTGCCTTTTATAAAAAACCCTT
CCCCAACAAAAGGATGGCGGGAAAAAGAAGGGTGCCTAAACAAAAGAAAG
AAATAAGAA
back to top