DT214534

Overview
NameDT214534
Unique NameDT214534
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length544
Libraries
Library NameType
Ruby Orange Developing Seed cDNA Library UCRCS09-2cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DT214534 vs. ExPASy Swiss-Prot
Match: SAT3_ARATH (Serine acetyltransferase 3, mitochondrial OS=Arabidopsis thaliana GN=SAT3 PE=1 SV=3)

HSP 1 Score: 172.17 bits (435), Expect = 1.459e-42
Identity = 84/114 (73.68%), Postives = 92/114 (80.70%), Query Frame = -2
Query:  202 VVVGETAVIGHNVSILHNVTLGGTGKMSGDRHPKIGNGVLVGAGTCILXXXXXXXXXXXXXGSVVLKDVPPRTTAVGNPARLIGGKENPFMLDKIPSFTMDHTSHIHEWSDYVI 543
            +V+GETAV+G+NVSILHNVTLGGTGK  GDRHPKIG+GVL+GAGTCIL             GSVVLKDVPPRTTAVGNPARL+GGK+NP   DKIP  TMD TSHI EWSDYVI
Sbjct:  278 IVIGETAVVGNNVSILHNVTLGGTGKQCGDRHPKIGDGVLIGAGTCILGNITIGEGAKIGAGSVVLKDVPPRTTAVGNPARLLGGKDNPKTHDKIPGLTMDQTSHISEWSDYVI 391          
BLAST of DT214534 vs. ExPASy Swiss-Prot
Match: SAT1_ARATH (Serine acetyltransferase 1 OS=Arabidopsis thaliana GN=SAT1 PE=1 SV=2)

HSP 1 Score: 163.696 bits (413), Expect = 5.188e-40
Identity = 82/114 (71.93%), Postives = 90/114 (78.95%), Query Frame = -2
Query:  202 VVVGETAVIGHNVSILHNVTLGGTGKMSGDRHPKIGNGVLVGAGTCILXXXXXXXXXXXXXGSVVLKDVPPRTTAVGNPARLIGGKENPFMLDKIPSFTMDHTSHIHEWSDYVI 543
            VV+GETAV+G NVSILH VTLGGTGK SGDRHPKIG+GVL+GAG+CIL             GSVV+KDVP RTTAVGNPARLIGGKENP   DKIP  TMD TS++ EWSDYVI
Sbjct:  201 VVIGETAVVGDNVSILHGVTLGGTGKQSGDRHPKIGDGVLIGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGNPARLIGGKENPRKHDKIPCLTMDQTSYLTEWSDYVI 314          
BLAST of DT214534 vs. ExPASy Swiss-Prot
Match: SAT5_ARATH (Serine acetyltransferase 5 OS=Arabidopsis thaliana GN=SAT5 PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.238e-33
Identity = 76/115 (66.09%), Postives = 85/115 (73.91%), Query Frame = -2
Query:  202 VVVGETAVIGHNVSILHNVTLGGTGKMSGDRHPKIGNGVLVGAGTCILXXXXXXXXXXXXXGSVVLKDVPPRTTAVGNPARLIGGKENPFMLD-KIPSFTMDHTSHIHEWSDYVI 543
            VVVGETAVIG+NVSILH+VTLGGTGK  GDRHPKIG+G L+GAG  IL             GSVVL DVP R TAVGNPARL+GGKE P + D + P  +MDHTS I EWSDY+I
Sbjct:  198 VVVGETAVIGNNVSILHHVTLGGTGKACGDRHPKIGDGCLIGAGATILGNVKIGAGAKVGAGSVVLIDVPCRGTAVGNPARLVGGKEKPTIHDEECPGESMDHTSFISEWSDYII 312          
BLAST of DT214534 vs. ExPASy Swiss-Prot
Match: SAT1_ORYSJ (Probable serine acetyltransferase 1 OS=Oryza sativa subsp. japonica GN=SAT1 PE=2 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 2.757e-33
Identity = 74/114 (64.91%), Postives = 84/114 (73.68%), Query Frame = -2
Query:  202 VVVGETAVIGHNVSILHNVTLGGTGKMSGDRHPKIGNGVLVGAGTCILXXXXXXXXXXXXXGSVVLKDVPPRTTAVGNPARLIGGKENPFMLDKIPSFTMDHTSHIHEWSDYVI 543
            VV+GETAVIG NVSILH+VTLGGTGK  GDRHPKIG+GVL+GAG  IL             GS+VL DVPPRTTAVGNPARL+GGK+     D +P  +MDHTS I +WSDY I
Sbjct:  194 VVIGETAVIGDNVSILHHVTLGGTGKAVGDRHPKIGDGVLIGAGATILGNVRIGAGAKIGAGSLVLIDVPPRTTAVGNPARLLGGKKG----DDMPGESMDHTSFIQQWSDYSI 303          
BLAST of DT214534 vs. ExPASy Swiss-Prot
Match: SAT5_ORYSJ (Probable serine acetyltransferase 5 OS=Oryza sativa subsp. japonica GN=SAT5 PE=2 SV=2)

HSP 1 Score: 134.806 bits (338), Expect = 2.581e-31
Identity = 72/115 (62.61%), Postives = 81/115 (70.43%), Query Frame = -2
Query:  202 VVVGETAVIGHNVSILHNVTLGGTGKMSGDRHPKIGNGVLVGAGTCILXXXXXXXXXXXXXGSVVLKDVPPRTTAVGNPARLIGGKENPFMLDK-IPSFTMDHTSHIHEWSDYVI 543
            VV+GETAV+G NVSILH+VTLGGTGK  GDRHPKIG+GVL+GAG  IL             GSVVL DVP R TAVGNPARLIG K      D+ +P  +MDHTS I +WSDY I
Sbjct:  226 VVIGETAVVGDNVSILHHVTLGGTGKAVGDRHPKIGDGVLIGAGATILGNVKIGAGAKIGAGSVVLIDVPARNTAVGNPARLIGRKNGEVEKDEDMPGESMDHTSFIRQWSDYTI 340          
BLAST of DT214534 vs. ExPASy Swiss-Prot
Match: SAT4_ORYSJ (Probable serine acetyltransferase 4 OS=Oryza sativa subsp. japonica GN=SAT4 PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.412e-23
Identity = 60/113 (53.10%), Postives = 76/113 (67.26%), Query Frame = -2
Query:  211 VVVGETAVIGHNVSILHNVTLGGTGKMSGDRHPKIGNGVLVGAGTCILXXXXXXXXXXXXXGSVVLKDVPPRTTAVGNPAR-LIGGKENPFML-DKIPSFTMDHTSHIHEWSD 543
            VV+GETAV+G++VSILH VTLGGTGK SGDRHPK+G+GVL+GAG  +L             G+VVL+DV   TTAVGNPA+ +IG K  P    +++P  TM+       WSD
Sbjct:  208 VVIGETAVVGYDVSILHGVTLGGTGKESGDRHPKVGDGVLIGAGASVLGNVHIGDGAKIGAGAVVLRDVADGTTAVGNPAKPIIGKKAAPQRRPEELPGVTME-----QRWSD 315          
BLAST of DT214534 vs. ExPASy Swiss-Prot
Match: SAT2_ORYSJ (Probable serine acetyltransferase 2 OS=Oryza sativa subsp. japonica GN=SAT2 PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.087e-21
Identity = 53/102 (51.96%), Postives = 69/102 (67.65%), Query Frame = -2
Query:  238 VVVGETAVIGHNVSILHNVTLGGTGKMSGDRHPKIGNGVLVGAGTCILXXXXXXXXXXXXXGSVVLKDVPPRTTAVGNPARLIGGKENPFMLDKIPSFTMDH 543
            +V+GETA++G+ VS++  VTLGGTGK +GDRHPKIG G L+GAG  IL             GS+VLKDVPP + AVGNPA+++G K+      + PS TM H
Sbjct:  233 LVIGETAIVGNWVSLMQGVTLGGTGKENGDRHPKIGQGALLGAGATILGNINVGEGAMIAAGSLVLKDVPPHSMAVGNPAKVVGYKDK-----EDPSLTMKH 329          
BLAST of DT214534 vs. ExPASy Swiss-Prot
Match: SAT4_ARATH (Serine acetyltransferase 4 OS=Arabidopsis thaliana GN=SAT4 PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 1.854e-21
Identity = 56/102 (54.90%), Postives = 67/102 (65.69%), Query Frame = -2
Query:  238 VVVGETAVIGHNVSILHNVTLGGTGKMSGDRHPKIGNGVLVGAGTCILXXXXXXXXXXXXXGSVVLKDVPPRTTAVGNPARLIGGKENPFMLDKIPSFTMDH 543
            VV+GETAVIG  VSILH VTLGGTGK +GDRHP IG+G L+GA   IL             GS+VLKDVP  +   GNPA+LIG     F+ ++ PS TM+H
Sbjct:  226 VVIGETAVIGDRVSILHGVTLGGTGKETGDRHPNIGDGALLGACVTILGNIKIGAGAMVAAGSLVLKDVPSHSMVAGNPAKLIG-----FVDEQDPSMTMEH 322          
BLAST of DT214534 vs. ExPASy Swiss-Prot
Match: CYSE_SALTY (Serine acetyltransferase OS=Salmonella typhimurium GN=cysE PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 7.045e-21
Identity = 61/113 (53.98%), Postives = 72/113 (63.72%), Query Frame = -2
Query:  208 VVVGETAVIGHNVSILHNVTLGGTGKMSGDRHPKIGNGVLVGAGTCILXXXXXXXXXXXXXGSVVLKDVPPRTTAVGNPARLIGGKENPFMLDKIPSFTMD-HTSHIHEWSDY 543
            +VVGETAVI  +VSIL +VTLGGTGK SGDRHPKI  GV++GAG  IL             GSVVL+ VPP TTA G PAR++G   +    DK PS  MD H + IH   +Y
Sbjct:  162 IVVGETAVIEDDVSILQSVTLGGTGKTSGDRHPKIREGVMIGAGAKILGNIEVGRGAKIGAGSVVLQPVPPHTTAAGVPARIVGKPGS----DK-PSMDMDQHFNGIHHTFEY 269          
BLAST of DT214534 vs. ExPASy Swiss-Prot
Match: CYSE_SHIFL (Serine acetyltransferase OS=Shigella flexneri GN=cysE PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.050e-20
Identity = 59/115 (51.30%), Postives = 72/115 (62.61%), Query Frame = -2
Query:  211 VVVGETAVIGHNVSILHNVTLGGTGKMSGDRHPKIGNGVLVGAGTCILXXXXXXXXXXXXXGSVVLKDVPPRTTAVGNPARLIGGKENPFMLDKIPSFTMDH----TSHIHEWSD 543
            +VVGETAVI ++VSIL +VTLGGTGK  GDRHPKI  GV++GAG  IL             GSVVL+ VPP TTA G PAR++G  ++    DK PS  MD      +H  E+ D
Sbjct:  162 IVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGAKIGAGSVVLQPVPPHTTAAGVPARIVGKPDS----DK-PSMDMDQHFNGINHTFEYGD 271          
The following BLAST results are available for this feature:
BLAST of DT214534 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 34
Match NameE-valueIdentityDescription
SAT3_ARATH1.459e-4273.68Serine acetyltransferase 3, mitochondrial OS=Arabi... [more]
SAT1_ARATH5.188e-4071.93Serine acetyltransferase 1 OS=Arabidopsis thaliana... [more]
SAT5_ARATH1.238e-3366.09Serine acetyltransferase 5 OS=Arabidopsis thaliana... [more]
SAT1_ORYSJ2.757e-3364.91Probable serine acetyltransferase 1 OS=Oryza sativ... [more]
SAT5_ORYSJ2.581e-3162.61Probable serine acetyltransferase 5 OS=Oryza sativ... [more]
SAT4_ORYSJ4.412e-2353.10Probable serine acetyltransferase 4 OS=Oryza sativ... [more]
SAT2_ORYSJ1.087e-2151.96Probable serine acetyltransferase 2 OS=Oryza sativ... [more]
SAT4_ARATH1.854e-2154.90Serine acetyltransferase 4 OS=Arabidopsis thaliana... [more]
CYSE_SALTY7.045e-2153.98Serine acetyltransferase OS=Salmonella typhimurium... [more]
CYSE_SHIFL2.050e-2051.30Serine acetyltransferase OS=Shigella flexneri GN=c... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS09_10D01_b2 Ruby Orange Developing Seed cDNA Library UCRCS09-2 Citrus sinensis cDNA clone UCRCS09-10D01-H1-1-4.d-polyt, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DT214534 ID=DT214534; Name=DT214534; organism=Citrus sinensis; type=EST; length=544bp
GTAATAGACTTGGCAACATCTTATGACCACACACCGAAAAAGCCGATCAA
AAACTACAACATAACATCAACAATGTTGCAAATCTAGCAACATACAATAA
TCTTAATAAACGACCTGTTTGCTAATGGGGAGAACTTCAGTTCTCACTCA
CAACTCAAGCAAAATCCACACTAACGAAGATGGAAACAAATTAGCCCTCT
AGATAACATAATCAGACCATTCATGTATATGTGATGTGTGGTCCATTGTG
AAGCTAGGGATTTTATCAAGCATGAATGGATTTTCCTTTCCCCCAATCAG
CCTAGCCGGGTTACCAACAGCAGTTGTTCTTGGTGGAACATCCTTTAAAA
CTACAGAACCAGCACCAATTTTAGCCCCATCACCGATCTTAATGTTCCCC
AAAATGCAAGTGCCTGCACCAACCAACACCCCGTTCCCAATTTTCGGATG
CCTATCACCAGACATTTTCCCTGTGCCCCCTAATGTAACATTGTGCAAAA
TTGACACATTATGTCCGATCACCGCCGTCTCTCCGACGACAACT
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