CN189319

Overview
NameCN189319
Unique NameCN189319
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length754
Libraries
Library NameType
Washington Navel Orange Stored Fruit Rind cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN189319 vs. ExPASy Swiss-Prot
Match: MDHP_ARATH (Malate dehydrogenase, chloroplastic OS=Arabidopsis thaliana GN=At3g47520 PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 4.864e-55
Identity = 123/189 (65.08%), Postives = 147/189 (77.78%), Query Frame = 2
Query:  191 SATTFSIRSTIAFG-PKAGQLPPSKPSGVRFNSKNSLVSFSGLKAVTSVICESNTSFLNKESCSALRSTFARKAQSSEQRPQYALQPQASFKVAVLGAAGGIGQPLALLIKMSPLVSALHLYDVMNVKGVAADLSHCNTPSQVLDFTGPEELASALKGVNIVVIPAGVPRKPGMTRDDLFNINANIVKT 754
            +AT+ S+ ST++    KA  +P S+   V+FNS   + SF+GLK+ TS+I  S++S L K     LR +   KAQ+S+++P Y  +  AS+KVAVLGAAGGIGQPL+LLIKMSPLVS LHLYD+ NVKGVAADLSHCNTPSQV DFTGP ELA  LK VN+VVIPAGVPRKPGMTRDDLFNINANIVKT
Sbjct:    3 TATSASLFSTVSSSYSKASSIPHSRLQSVKFNS---VPSFTGLKS-TSLISGSDSSSLAK----TLRGSVT-KAQTSDKKP-YGFKINASYKVAVLGAAGGIGQPLSLLIKMSPLVSTLHLYDIANVKGVAADLSHCNTPSQVRDFTGPSELADCLKDVNVVVIPAGVPRKPGMTRDDLFNINANIVKT 181          
BLAST of CN189319 vs. ExPASy Swiss-Prot
Match: MDHM_BRANA (Malate dehydrogenase, mitochondrial OS=Brassica napus GN=MDH PE=2 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.627e-34
Identity = 75/125 (60.00%), Postives = 94/125 (75.20%), Query Frame = 2
Query:  386 RSTFARKAQSSEQ---RPQYALQPQASFKVAVLGAAGGIGQPLALLIKMSPLVSALHLYDVMNVKGVAADLSHCNTPSQVLDFTGPEELASALKGVNIVVIPAGVPRKPGMTRDDLFNINANIVK 751
            RS   R + S++Q   R  ++       KVA+LGAAGGIGQPLALL+K++PLVS+L LYD+ N  GVAAD+ H NT SQV+ + G + LA AL+G ++V+IPAGVPRKPGMTRDDLFNINA IVK
Sbjct:    3 RSALVRSSASAKQSLLRRSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIANTPGVAADVGHINTRSQVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVK 127          
BLAST of CN189319 vs. ExPASy Swiss-Prot
Match: MDHM_FRAAN (Malate dehydrogenase, mitochondrial OS=Fragaria ananassa GN=MMDHI PE=1 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 2.124e-34
Identity = 79/132 (59.85%), Postives = 96/132 (72.73%), Query Frame = 2
Query:  365 KESCSALRST--FARKAQSSEQRPQYALQPQASFKVAVLGAAGGIGQPLALLIKMSPLVSALHLYDVMNVKGVAADLSHCNTPSQVLDFTGPEELASALKGVNIVVIPAGVPRKPGMTRDDLFNINANIVKT 754
            + S S +RS    AR+  SSE  PQ         KVAVLGAAGGIGQPLALL+K++PLVS L LYD+    GVAAD+SH NT S+V  + G E+L  AL+G ++V+IPAGVPRKPGMTRDDLFNINA IV++
Sbjct:    2 RPSMSLIRSVSRVARRGYSSESVPQR--------KVAVLGAAGGIGQPLALLMKLNPLVSQLSLYDIAGTPGVAADVSHINTRSEVKGYAGEEQLGEALEGCDVVIIPAGVPRKPGMTRDDLFNINAGIVRS 125          
BLAST of CN189319 vs. ExPASy Swiss-Prot
Match: MDHG_ORYSJ (Malate dehydrogenase, glyoxysomal OS=Oryza sativa subsp. japonica GN=Os12g0632700 PE=1 SV=3)

HSP 1 Score: 145.206 bits (365), Expect = 3.624e-34
Identity = 70/99 (70.71%), Postives = 84/99 (84.85%), Query Frame = 2
Query:  458 FKVAVLGAAGGIGQPLALLIKMSPLVSALHLYDVMNVKGVAADLSHCNTPSQVLDFTGPEELASALKGVNIVVIPAGVPRKPGMTRDDLFNINANIVKT 754
            FKVA+LGA+GGIGQPLALL+KM+PLVS LHLYDV+N  GV AD+SH NT + V  F G  +L +AL G+++V+IPAGVPRKPGMTRDDLFNINA IV+T
Sbjct:   45 FKVAILGASGGIGQPLALLMKMNPLVSVLHLYDVVNTPGVTADISHMNTGAVVRGFLGQPQLENALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRT 143          
BLAST of CN189319 vs. ExPASy Swiss-Prot
Match: MDHM1_ARATH (Malate dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana GN=At1g53240 PE=1 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 4.733e-34
Identity = 74/125 (59.20%), Postives = 94/125 (75.20%), Query Frame = 2
Query:  386 RSTFARKAQSSEQ---RPQYALQPQASFKVAVLGAAGGIGQPLALLIKMSPLVSALHLYDVMNVKGVAADLSHCNTPSQVLDFTGPEELASALKGVNIVVIPAGVPRKPGMTRDDLFNINANIVK 751
            RS   R + S++Q   R  ++       KVA+LGAAGGIGQPLALL+K++PLVS+L LYD+ N  GVAAD+ H NT S+V+ + G + LA AL+G ++V+IPAGVPRKPGMTRDDLFNINA IVK
Sbjct:    3 RSMLVRSSASAKQAVIRRSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIANTPGVAADVGHINTRSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIVK 127          
BLAST of CN189319 vs. ExPASy Swiss-Prot
Match: MDHM_EUCGU (Malate dehydrogenase, mitochondrial OS=Eucalyptus gunnii GN=MDH PE=2 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 6.181e-34
Identity = 76/131 (58.02%), Postives = 94/131 (71.76%), Query Frame = 2
Query:  362 NKESCSALRSTFARKAQSSEQRPQYALQPQASFKVAVLGAAGGIGQPLALLIKMSPLVSALHLYDVMNVKGVAADLSHCNTPSQVLDFTGPEELASALKGVNIVVIPAGVPRKPGMTRDDLFNINANIVKT 754
            ++ S +A R    R+A  SE  P+         KVAVLGAAGGIGQPLALL+K++PLVS L LYD+    GVAAD+ H NT S+V  + G E+L  AL+G ++V+IPAGVPRKPGMTRDDLFNINA IVK+
Sbjct:   11 SRSSSAAPRPHLLRRAYGSESVPER--------KVAVLGAAGGIGQPLALLMKLNPLVSQLALYDIAGTPGVAADVGHINTRSEVAGYVGEEQLGQALEGSDVVIIPAGVPRKPGMTRDDLFNINAGIVKS 133          
BLAST of CN189319 vs. ExPASy Swiss-Prot
Match: MDHG_SOYBN (Malate dehydrogenase, glyoxysomal OS=Glycine max PE=2 SV=2)

HSP 1 Score: 143.665 bits (361), Expect = 1.054e-33
Identity = 69/101 (68.32%), Postives = 85/101 (84.16%), Query Frame = 2
Query:  452 ASFKVAVLGAAGGIGQPLALLIKMSPLVSALHLYDVMNVKGVAADLSHCNTPSQVLDFTGPEELASALKGVNIVVIPAGVPRKPGMTRDDLFNINANIVKT 754
            + FKVA+LGAAGGIGQPLA+L+KM+PLVS LHLYDV+N  GV +D+SH +T + V  F G ++L  AL G+++V+IPAGVPRKPGMTRDDLFNINA IVKT
Sbjct:   40 SGFKVAILGAAGGIGQPLAMLMKMNPLVSLLHLYDVVNTPGVTSDISHMDTGAVVRGFLGQQQLEDALIGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKT 140          
BLAST of CN189319 vs. ExPASy Swiss-Prot
Match: MDHG_CUCSA (Malate dehydrogenase, glyoxysomal OS=Cucumis sativus GN=MDHG PE=2 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.377e-33
Identity = 70/99 (70.71%), Postives = 83/99 (83.84%), Query Frame = 2
Query:  458 FKVAVLGAAGGIGQPLALLIKMSPLVSALHLYDVMNVKGVAADLSHCNTPSQVLDFTGPEELASALKGVNIVVIPAGVPRKPGMTRDDLFNINANIVKT 754
            FKVA+LGAAGGIGQPLA+L+KM+PLVS LHLYDV+N  GV AD+SH +T + V  F G ++L  AL G+++VVIPAGVPRKPGMTRDDLF INA IVKT
Sbjct:   45 FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVVNAPGVTADISHMDTGAVVRGFLGQQQLERALTGMDLVVIPAGVPRKPGMTRDDLFKINAGIVKT 143          
BLAST of CN189319 vs. ExPASy Swiss-Prot
Match: MDHG_CITLA (Malate dehydrogenase, glyoxysomal OS=Citrullus lanatus PE=1 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.798e-33
Identity = 67/99 (67.68%), Postives = 84/99 (84.85%), Query Frame = 2
Query:  458 FKVAVLGAAGGIGQPLALLIKMSPLVSALHLYDVMNVKGVAADLSHCNTPSQVLDFTGPEELASALKGVNIVVIPAGVPRKPGMTRDDLFNINANIVKT 754
            FKVA+LGAAGGIGQPLA+L+KM+PLVS LHLYDV+N  GV AD+SH +T + V  F G ++L +AL G++++++PAGVPRKPGMTRDDLF INA IVKT
Sbjct:   45 FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVVNAPGVTADISHMDTGAVVRGFLGQQQLEAALTGMDLIIVPAGVPRKPGMTRDDLFKINAGIVKT 143          
BLAST of CN189319 vs. ExPASy Swiss-Prot
Match: MDHG2_ARATH (Probable malate dehydrogenase, glyoxysomal OS=Arabidopsis thaliana GN=At2g22780 PE=1 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.798e-33
Identity = 69/99 (69.70%), Postives = 82/99 (82.83%), Query Frame = 2
Query:  458 FKVAVLGAAGGIGQPLALLIKMSPLVSALHLYDVMNVKGVAADLSHCNTPSQVLDFTGPEELASALKGVNIVVIPAGVPRKPGMTRDDLFNINANIVKT 754
            FKVA+LGAAGGIGQPLA+L+KM+PLVS LHLYDV N  GV AD+SH +T + V  F G  +L  AL G+++V+IPAGVPRKPGMTRDDLFNINA IV+T
Sbjct:   43 FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVANAPGVTADISHMDTSAVVRGFLGQPQLEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRT 141          
The following BLAST results are available for this feature:
BLAST of CN189319 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 154
Match NameE-valueIdentityDescription
MDHP_ARATH4.864e-5565.08Malate dehydrogenase, chloroplastic OS=Arabidopsis... [more]
MDHM_BRANA1.627e-3460.00Malate dehydrogenase, mitochondrial OS=Brassica na... [more]
MDHM_FRAAN2.124e-3459.85Malate dehydrogenase, mitochondrial OS=Fragaria an... [more]
MDHG_ORYSJ3.624e-3470.71Malate dehydrogenase, glyoxysomal OS=Oryza sativa ... [more]
MDHM1_ARATH4.733e-3459.20Malate dehydrogenase 1, mitochondrial OS=Arabidops... [more]
MDHM_EUCGU6.181e-3458.02Malate dehydrogenase, mitochondrial OS=Eucalyptus ... [more]
MDHG_SOYBN1.054e-3368.32Malate dehydrogenase, glyoxysomal OS=Glycine max P... [more]
MDHG_CUCSA1.377e-3370.71Malate dehydrogenase, glyoxysomal OS=Cucumis sativ... [more]
MDHG_CITLA1.798e-3367.68Malate dehydrogenase, glyoxysomal OS=Citrullus lan... [more]
MDHG2_ARATH1.798e-3369.70Probable malate dehydrogenase, glyoxysomal OS=Arab... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS06_0001K19_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0001K19, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN189319 ID=CN189319; Name=CN189319; organism=Citrus sinensis; type=EST; length=754bp
GACGGCTTTCGAAAAAGCGTAGATTCGTTCAAGGCCCATTCGGCGGAGGA
AGAGACACCCAGTTTACAATAACCCTTTCAAAAATCTTCTTCTTCTTCTT
CTCTCTTTCCCCTCAACGCCAGCAGCAACACCACCATCTCTTCCAGCTTC
ACGCAAATCTCAAGTGTCAGAGTGTGAAATGGCCGCTACATCAGCTACAA
CCTTTTCAATTAGATCAACTATTGCCTTTGGCCCCAAGGCAGGCCAACTT
CCACCGTCAAAACCCTCTGGTGTGAGGTTCAACTCCAAGAACTCACTTGT
GAGTTTCAGTGGCCTCAAGGCAGTGACATCAGTTATCTGTGAATCAAATA
CCTCTTTCTTGAACAAGGAGAGTTGTTCAGCTCTTCGAAGCACTTTTGCA
AGAAAAGCCCAAAGTTCAGAGCAGAGGCCTCAGTATGCCCTACAGCCCCA
GGCTTCTTTTAAAGTAGCAGTTCTTGGAGCTGCTGGTGGAATAGGTCAAC
CCTTAGCACTTCTAATCAAGATGTCCCCACTAGTATCAGCCCTTCACCTC
TATGATGTAATGAATGTCAAGGGAGTTGCTGCTGACCTCAGTCACTGCAA
CACTCCCTCTCAAGTTCTGGATTTCACAGGACCTGAAGAATTAGCCAGTG
CTTTGAAAGGGGTGAATATCGTCGTCATACCTGCTGGAGTTCCAAGAAAG
CCTGGGATGACCCGTGATGACCTCTTCAACATCAACGCCAACATAGTAAA
GACC
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