CN189314

Overview
NameCN189314
Unique NameCN189314
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length665
Libraries
Library NameType
Washington Navel Orange Stored Fruit Rind cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN189314 vs. ExPASy Swiss-Prot
Match: BCAT2_ARATH (Branched-chain-amino-acid aminotransferase 2, chloroplastic OS=Arabidopsis thaliana GN=BCAT2 PE=1 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 3.474e-64
Identity = 120/159 (75.47%), Postives = 139/159 (87.42%), Query Frame = -2
Query:  176 GGAGGVKAISNYAPVLKALIRAKSRGFSDVLYLDSVNRKNVEEVSSCNIFVVKGNVISTPATNGTILEGITRRSVIEIARDYGYQVEERVIPVDELINADQVFCTGTAVVVAPVGSITYQNKRVEFKTGSRSVYQELYSTLVGIQTGVIEDKKGWTVEV 652
            GGAGGVK+I+NYAPVLKAL RAKSRGFSDVLYLDSV +K +EE SSCN+FVVKG  ISTPATNGTILEGITR+SV+EIA D GYQV E+ + VDE+++AD+VFCTGTAVVVAPVG+ITYQ KRVE+KTG  SV Q+L S LVGIQTG+IED KGW  ++
Sbjct:  229 GGAGGVKSITNYAPVLKALSRAKSRGFSDVLYLDSVKKKYLEEASSCNVFVVKGRTISTPATNGTILEGITRKSVMEIASDQGYQVVEKAVHVDEVMDADEVFCTGTAVVVAPVGTITYQEKRVEYKTGDESVCQKLRSVLVGIQTGLIEDNKGWVTDI 387          
BLAST of CN189314 vs. ExPASy Swiss-Prot
Match: BCAT1_ARATH (Branched-chain-amino-acid aminotransferase 1, mitochondrial OS=Arabidopsis thaliana GN=BCAT1 PE=1 SV=2)

HSP 1 Score: 211.075 bits (536), Expect = 4.257e-54
Identity = 105/161 (65.22%), Postives = 128/161 (79.50%), Query Frame = -2
Query:  176 FCGGAGGVKAISNYAPVLKALIRAKSRGFSDVLYLDSVNRKNVEEVSSCNIFVVKGNVISTPATNGTILEGITRRSVIEIARDYGYQVEERVIPVDELINADQVFCTGTAVVVAPVGSITYQNKRVEFKTGSRSVYQELYSTLVGIQTGVIEDKKGWTVEV 658
            + GG GGVKAISNY PVL+ + RAKSRGFSDVLYLD+   KN+EEVS+ NIF+VKGN I TPAT+GTIL GITR+S+IEIA D GY+VEER +PV+EL  A++VFCTGTA  VA VGSIT++N R E+K G   V Q+L S LVGIQTG I+D K W +++
Sbjct:  223 YLGGTGGVKAISNYGPVLEVMRRAKSRGFSDVLYLDADTGKNIEEVSAANIFLVKGNTIVTPATSGTILGGITRKSIIEIALDLGYKVEERSVPVEELKEAEEVFCTGTAAGVASVGSITFKNTRTEYKVGDGIVTQQLRSILVGIQTGSIQDTKDWVLQI 383          
BLAST of CN189314 vs. ExPASy Swiss-Prot
Match: BCAT7_ARATH (Putative branched-chain-amino-acid aminotransferase 7 OS=Arabidopsis thaliana GN=BCAT7 PE=5 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 4.707e-53
Identity = 95/162 (58.64%), Postives = 132/162 (81.48%), Query Frame = -2
Query:  176 HFCGGAGGVKAISNYAPVLKALIRAKSRGFSDVLYLDSVNRKNVEEVSSCNIFVVKGNVISTPATNGTILEGITRRSVIEIARDYGYQVEERVIPVDELINADQVFCTGTAVVVAPVGSITYQNKRVEFKTGSRSVYQELYSTLVGIQTGVIEDKKGWTVEV 661
            HF GG GGVK+ +NY+PV+K+LI AKS GFSDVL+LD+   KN+EEVS+CNIF++KGN++STP T+GTIL GITR+S+ E+ARD GY+V+ER + VDEL+ A++VFCTGTAVV+  V ++T+ +KRV+++TG  +   +L+  L  IQ GV+EDKKGW +E+
Sbjct:  194 HF-GGTGGVKSCTNYSPVVKSLIEAKSSGFSDVLFLDAATGKNIEEVSTCNIFILKGNIVSTPPTSGTILPGITRKSICELARDIGYEVQERDLSVDELLEAEEVFCTGTAVVIKAVETVTFHDKRVKYRTGEEAFSTKLHLILTNIQMGVVEDKKGWMMEI 354          
BLAST of CN189314 vs. ExPASy Swiss-Prot
Match: BCAT6_ARATH (Branched-chain-amino-acid aminotransferase 6 OS=Arabidopsis thaliana GN=BCAT6 PE=1 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 1.788e-52
Identity = 91/159 (57.23%), Postives = 131/159 (82.39%), Query Frame = -2
Query:  176 GGAGGVKAISNYAPVLKALIRAKSRGFSDVLYLDSVNRKNVEEVSSCNIFVVKGNVISTPATNGTILEGITRRSVIEIARDYGYQVEERVIPVDELINADQVFCTGTAVVVAPVGSITYQNKRVEFKTGSRSVYQELYSTLVGIQTGVIEDKKGWTVEV 652
            GG GGVK+ +NY+PV+K+L+ AKS GFSDVL+LD+   +N+EE+++CNIF+VKGN++STP T+GTIL G+TR+S+ E+A D GYQVEER + VDEL+ A++VFCTGTAVVV  V ++T+ +K+V+++TG  ++  +L+S L  IQ GV+EDKKGW V++
Sbjct:  193 GGTGGVKSCTNYSPVVKSLLEAKSAGFSDVLFLDAATGRNIEELTACNIFIVKGNIVSTPPTSGTILPGVTRKSISELAHDIGYQVEERDVSVDELLEAEEVFCTGTAVVVKAVETVTFHDKKVKYRTGEAALSTKLHSMLTNIQMGVVEDKKGWMVDI 351          
BLAST of CN189314 vs. ExPASy Swiss-Prot
Match: BCAT3_ARATH (Branched-chain-amino-acid aminotransferase 3, chloroplastic OS=Arabidopsis thaliana GN=BCAT3 PE=1 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 1.085e-49
Identity = 97/160 (60.62%), Postives = 125/160 (78.12%), Query Frame = -2
Query:  176 GGAGGVKAISNYAPVLKALIRAKSRGFSDVLYLDSVNRKNVEEVSSCNIFVVKGNVISTPATNGTILEGITRRSVIEIARDYGYQVEERVIPVDELINADQVFCTGTAVVVAPVGSITYQNKRVEFKTGS-RSVYQELYSTLVGIQTGVIEDKKGWTVEV 652
            GG GGVK I NYA VLKA   AK++G+SDVLYLD + ++ +EEVSSCNIF+VK NVISTP   GTIL GITR+S+I++AR  G+QVEER + VDEL+ AD+VFCTGTAVVV+PVGS+TY+ KRV +  G+  +V ++LY+ L  +Q G+IED   WTV +
Sbjct:  253 GGTGGVKTIGNYAAVLKAQSIAKAKGYSDVLYLDCIYKRYLEEVSSCNIFIVKDNVISTPEIKGTILPGITRKSMIDVARTQGFQVEERNVTVDELLEADEVFCTGTAVVVSPVGSVTYKGKRVSYGEGTFGTVSKQLYTVLTSLQMGLIEDNMKWTVNL 412          
BLAST of CN189314 vs. ExPASy Swiss-Prot
Match: BCAT5_ARATH (Branched-chain-amino-acid aminotransferase 5, chloroplastic OS=Arabidopsis thaliana GN=BCAT5 PE=1 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 7.033e-49
Identity = 94/160 (58.75%), Postives = 127/160 (79.38%), Query Frame = -2
Query:  176 GGAGGVKAISNYAPVLKALIRAKSRGFSDVLYLDSVNRKNVEEVSSCNIFVVKGNVISTPATNGTILEGITRRSVIEIARDYGYQVEERVIPVDELINADQVFCTGTAVVVAPVGSITYQNKRVEF-KTGSRSVYQELYSTLVGIQTGVIEDKKGWTVEV 652
            GG GGVK I NYA VLKA   AK++G+SDVLYLD ++++ +EEVSSCNIF+VK NVISTP   GTIL GITR+S+IE+AR  G++VEER + VDEL+ AD+VFCTGTAVV++PVGSITY+++R  + + G  +V ++LY++L  +Q G+ ED   WTV++
Sbjct:  255 GGTGGVKTIGNYAAVLKAQSIAKAKGYSDVLYLDCLHKRYLEEVSSCNIFIVKDNVISTPEIKGTILPGITRKSIIEVARSQGFKVEERNVTVDELVEADEVFCTGTAVVLSPVGSITYKSQRFSYGEDGFGTVSKQLYTSLTSLQMGLSEDNMNWTVQL 414          
BLAST of CN189314 vs. ExPASy Swiss-Prot
Match: BCAT4_ARATH (Probable branched-chain-amino-acid aminotransferase 4 OS=Arabidopsis thaliana GN=BCAT4 PE=2 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 5.954e-48
Identity = 86/161 (53.42%), Postives = 120/161 (74.53%), Query Frame = -2
Query:  176 FCGGAGGVKAISNYAPVLKALIRAKSRGFSDVLYLDSVNRKNVEEVSSCNIFVVKGNVISTPATNGTILEGITRRSVIEIARDYGYQVEERVIPVDELINADQVFCTGTAVVVAPVGSITYQNKRVEFKTGSRSVYQELYSTLVGIQTGVIEDKKGWTVEV 658
            F  G GGVK+I+NY PV   L  AK +GFSD+L+LD+   KN+EE+ + N+F++KGNV+STP   GTIL G+TR  V+E+ RD+GYQVEER IP+ + ++AD+ FCTGTA +V  + S+T+++K+  FKTG  ++  +LY TL  IQTG +ED KGWTVE+
Sbjct:  190 FPSGTGGVKSITNYCPVWIPLAEAKKQGFSDILFLDAATGKNIEELFAANVFMLKGNVVSTPTIAGTILPGVTRNCVMELCRDFGYQVEERTIPLVDFLDADEAFCTGTASIVTSIASVTFKDKKTGFKTGEETLAAKLYETLSDIQTGRVEDTKGWTVEI 350          
BLAST of CN189314 vs. ExPASy Swiss-Prot
Match: ILVE_STAES (Probable branched-chain-amino-acid aminotransferase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=ilvE PE=3 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 2.595e-35
Identity = 78/168 (46.43%), Postives = 106/168 (63.10%), Query Frame = -2
Query:  170 GGAGGVKAISNYAPVLKALIRAKSRGFSDVLYLDSVNRKNVEEVSSCNIFVVKGNVISTPATNGTILEGITRRSVIEIARDYGYQVEERVIPVDELINA------DQVFCTGTAVVVAPVGSITYQNKRVEFKTGS-RSVYQELYSTLVGIQTGVIEDKKGWTVEV*K 652
            GG G  K   NYA  L A   A   G+  VL+LD V +K VEEV S NIF V+   + TPA NG+IL GITR+S+I++A D GY+VEER + ++EL NA       +VF +GTA V++PVG++ Y+++ +         + Q+LY T  GIQ+G +EDK GW VEV K
Sbjct:  190 GGVGFAKVAGNYAASLLAQTNANKLGYDQVLWLDGVEQKYVEEVGSMNIFFVENGKVVTPALNGSILPGITRKSIIQLAEDLGYEVEERRVSIEELFNAYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNEPGKITQKLYDTYTGIQSGKLEDKYGWRVEVPK 357          
BLAST of CN189314 vs. ExPASy Swiss-Prot
Match: ILVE_STAEQ (Probable branched-chain-amino-acid aminotransferase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=ilvE PE=3 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 2.595e-35
Identity = 78/168 (46.43%), Postives = 106/168 (63.10%), Query Frame = -2
Query:  170 GGAGGVKAISNYAPVLKALIRAKSRGFSDVLYLDSVNRKNVEEVSSCNIFVVKGNVISTPATNGTILEGITRRSVIEIARDYGYQVEERVIPVDELINA------DQVFCTGTAVVVAPVGSITYQNKRVEFKTGS-RSVYQELYSTLVGIQTGVIEDKKGWTVEV*K 652
            GG G  K   NYA  L A   A   G+  VL+LD V +K VEEV S NIF V+   + TPA NG+IL GITR+S+I++A D GY+VEER + ++EL NA       +VF +GTA V++PVG++ Y+++ +         + Q+LY T  GIQ+G +EDK GW VEV K
Sbjct:  190 GGVGFAKVAGNYAASLLAQTNANKLGYDQVLWLDGVEQKYVEEVGSMNIFFVENGKVVTPALNGSILPGITRKSIIQLAEDLGYEVEERRVSIEELFNAYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNEPGKITQKLYDTYTGIQSGKLEDKYGWRVEVPK 357          
BLAST of CN189314 vs. ExPASy Swiss-Prot
Match: ILVE2_BACSU (Branched-chain-amino-acid aminotransferase 2 OS=Bacillus subtilis GN=ilvK PE=1 SV=5)

HSP 1 Score: 142.124 bits (357), Expect = 2.428e-33
Identity = 75/166 (45.18%), Postives = 103/166 (62.05%), Query Frame = -2
Query:  176 GGAGGVKAISNYAPVLKALIRAKSRGFSDVLYLDSVNRKNVEEVSSCNIFVVKGNVISTPATNGTILEGITRRSVIEIARDYGYQVEERVIPVDELINA------DQVFCTGTAVVVAPVGSITYQNKRVEFKTG-SRSVYQELYSTLVGIQTGVIEDKKGWTVEV 652
            GG G  K   NYA  LKA   A+ +GFS VL+LD + +K +EEV S NIF      I TP  NG+ILEGITR SVI + + +G QV ER I +DE+I A      ++ F TGTA V++PVG + +Q++ +    G +  + ++LY T+ GIQ G + D+ GWT EV
Sbjct:  191 GGTGNAKTAGNYASSLKAQQVAEEKGFSQVLWLDGIEKKYIEEVGSMNIFFKINGEIVTPMLNGSILEGITRNSVIALLKHWGLQVSERKIAIDEVIQAHKDGILEEAFGTGTAAVISPVGELIWQDETLSINNGETGEIAKKLYDTITGIQKGAVADEFGWTTEV 356          
The following BLAST results are available for this feature:
BLAST of CN189314 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 52
Match NameE-valueIdentityDescription
BCAT2_ARATH3.474e-6475.47Branched-chain-amino-acid aminotransferase 2, chlo... [more]
BCAT1_ARATH4.257e-5465.22Branched-chain-amino-acid aminotransferase 1, mito... [more]
BCAT7_ARATH4.707e-5358.64Putative branched-chain-amino-acid aminotransferas... [more]
BCAT6_ARATH1.788e-5257.23Branched-chain-amino-acid aminotransferase 6 OS=Ar... [more]
BCAT3_ARATH1.085e-4960.63Branched-chain-amino-acid aminotransferase 3, chlo... [more]
BCAT5_ARATH7.033e-4958.75Branched-chain-amino-acid aminotransferase 5, chlo... [more]
BCAT4_ARATH5.954e-4853.42Probable branched-chain-amino-acid aminotransferas... [more]
ILVE_STAES2.595e-3546.43Probable branched-chain-amino-acid aminotransferas... [more]
ILVE_STAEQ2.595e-3546.43Probable branched-chain-amino-acid aminotransferas... [more]
ILVE2_BACSU2.428e-3345.18Branched-chain-amino-acid aminotransferase 2 OS=Ba... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS06_0001K17_f Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0001K17, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN189314 ID=CN189314; Name=CN189314; organism=Citrus sinensis; type=EST; length=665bp
TTTTTTTTTTTTTTTAATGTTCATATTTCTTTCATTTATTACCATCAGCT
TTGATGGGATTTAAAAATACATGGAAACTTTTTTTTTTACCTTACATAAT
ATGGGGCCAACAGCATTTTGTAACGACATTCTGTAAGTAAGCATAGCATT
ATTGGTGTTTAAACTTTGATTTTTAAACCTCCACAGTCCATCCTTTCTTA
TCCTCAATAACACCCGTTTGAATTCCTACGAGAGTTGAGTACAATTCCTG
ATACACCGAGCGTGACCCTGTTTTAAATTCAACTCTTTTATTTTGATATG
TGATACTGCCCACTGGAGCAACAACTACAGCGGTTCCAGTGCAAAATACT
TGGTCGGCATTGATCAGTTCGTCCACAGGAATAACACGTTCCTCAACCTG
ATAGCCATAATCCCTGGCAATTTCAATTACACTTCTTCTGGTAATCCCTT
CAAGAATAGTTCCATTTGTAGCGGGAGTTGAAATTACATTGCCCTTTACA
ACAAAAATGTTACAAGAAGAGACTTCCTCCACATTTTTCCTATTGACCGA
GTCAAGGTACAAGACATCCGAAAATCCTCTGCTCTTGGCTCGGATTAATG
CTTTCAGAACAGGAGCATAATTGCTGATTGCTTTTACCCCTCCAGCGCCG
CCGCAAAAATGAAAT
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