CX295642

Overview
NameCX295642
Unique NameCX295642
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length736
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295642 vs. ExPASy Swiss-Prot
Match: IFRH_SOLTU (Isoflavone reductase homolog OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 343.584 bits (880), Expect = 6.638e-94
Identity = 172/216 (79.63%), Postives = 190/216 (87.96%), Query Frame = 1
Query:   67 MASKSKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSDPSKSQLLDHFKKLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEPAKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPKAVYNKEDDIGTYTIKAVDDPRTLNKSFTLTP 714
            MA KSKILFIGGTGYIGKFIVEASAKAGH TFVLVRESTLS+P+K++L+D FK  GV  V GD+ +HESLVKAIKQVDVVISTVGH LL DQVK+IAAIKEAGN+KRFFPSEFGNDVDRVHAVEPAK+AF TKA+IRR VEAEGIP+TYVA+ FFA Y LPNL+QPGA  PP DKVVILG GN KAV+NKE+DIGTYTI AVDDP+TLNK   + P
Sbjct:    1 MAGKSKILFIGGTGYIGKFIVEASAKAGHDTFVLVRESTLSNPTKTKLIDTFKSFGVTFVHGDLYDHESLVKAIKQVDVVISTVGHALLADQVKLIAAIKEAGNVKRFFPSEFGNDVDRVHAVEPAKAAFNTKAQIRRVVEAEGIPFTYVATFFFAGYSLPNLAQPGAAGPPNDKVVILGHGNTKAVFNKEEDIGTYTINAVDDPKTLNKILYIKP 216          
BLAST of CX295642 vs. ExPASy Swiss-Prot
Match: IFRH_TOBAC (Isoflavone reductase homolog A622 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 303.523 bits (776), Expect = 7.613e-82
Identity = 151/217 (69.59%), Postives = 173/217 (79.72%), Query Frame = 1
Query:   64 VMASKSKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSDPSKSQLLDHFKKLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEPAKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPKAVYNKEDDIGTYTIKAVDDPRTLNKSFTLTP 714
            V++ KSKIL IGGTGYIGK++VE SAK+GHPTF L+RESTL +P KS+L+D FK  GV L+ GD+ N ESL+KAIKQVDVVISTVG     DQV II AIKEAGNIKRF PSEFG DVD   A+EPA S FA K +IRR +EAEGIPYTYV  N+FA +FLPNL Q  A  PPRDKVVI GDGNPKA+Y KE+DI TYTI+AVDDPRTLNK+  + P
Sbjct:    2 VVSEKSKILIIGGTGYIGKYLVETSAKSGHPTFALIRESTLKNPEKSKLIDTFKSYGVTLLFGDISNQESLLKAIKQVDVVISTVGGQQFTDQVNIIKAIKEAGNIKRFLPSEFGFDVDHARAIEPAASLFALKVRIRRMIEAEGIPYTYVICNWFADFFLPNLGQLEAKTPPRDKVVIFGDGNPKAIYVKEEDIATYTIEAVDDPRTLNKTLHMRP 218          
BLAST of CX295642 vs. ExPASy Swiss-Prot
Match: IFRH_ARATH (Isoflavone reductase homolog P3 OS=Arabidopsis thaliana GN=At1g75280 PE=1 SV=1)

HSP 1 Score: 296.59 bits (758), Expect = 9.306e-80
Identity = 153/215 (71.16%), Postives = 171/215 (79.53%), Query Frame = 1
Query:   76 KSKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSDPSKSQLLDHFKKLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEPAKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQ--PGATAPPRDKVVILGDGNPKAVYNKEDDIGTYTIKAVDDPRTLNKSFTLTP 714
            KSKIL IGGTGYIGKF+VEASAKAGH TF LVRE+TLSDP K + +  FK LGV ++ GD+ +HESLVKAIKQVDVVISTVG   + DQ KII+AIKEAGN+KRF PSEFG DVDR  AVEPAKSAFA K +IRR +EAEGIPYTY  +  F  Y+LP L Q  PG T+PPRDKV ILGDGN KAV NKE+DI  YTIKAVDDPRTLNK   + P
Sbjct:    5 KSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLNDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSAVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGDGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKP 219          
BLAST of CX295642 vs. ExPASy Swiss-Prot
Match: IFR_MEDSA (Isoflavone reductase OS=Medicago sativa GN=IFR PE=1 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 1.104e-64
Identity = 127/222 (57.21%), Postives = 163/222 (73.42%), Query Frame = 1
Query:   67 MASKSKILFIGGTGYIGKFIVEASAKAGHPTFVLVRES----------TLSDP-SKSQLLDHFKKLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEPAKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPKAVYNKEDDIGTYTIKAVDDPRTLNKS 699
            MA+++KIL +G TG IG+ IV AS KAG+PT+ LVR++          T ++P +K +L+D+++ LGV L+ GD+ +HE+LVKAIKQVD+VI   G  L+ DQVKII AIKEAGN+K+FFPSEFG DVDR  AVEP +  F  KA IRR +EAEG+PYTY+  + F  YFL NL+Q   T PPRDKVVILGDGN K  Y  E D+GT+TI+A +DP TLNK+
Sbjct:    1 MATENKILILGPTGAIGRHIVWASIKAGNPTYALVRKTPGNVNKPKLITAANPETKEELIDNYQSLGVILLEGDINDHETLVKAIKQVDIVICAAGRLLIEDQVKIIKAIKEAGNVKKFFPSEFGLDVDRHEAVEPVRQVFEEKASIRRVIEAEGVPYTYLCCHAFTGYFLRNLAQLDTTDPPRDKVVILGDGNVKGAYVTEADVGTFTIRAANDPNTLNKA 222          
BLAST of CX295642 vs. ExPASy Swiss-Prot
Match: IFR_CICAR (Isoflavone reductase OS=Cicer arietinum GN=IFR PE=1 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 1.884e-64
Identity = 129/222 (58.11%), Postives = 159/222 (71.62%), Query Frame = 1
Query:   67 MASKSKILFIGGTGYIGKFIVEASAKAGHPTFVLVRES-----------TLSDPSKSQLLDHFKKLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEPAKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPKAVYNKEDDIGTYTIKAVDDPRTLNKS 699
            MAS+++IL +G TG IG+ +V AS KAG+PT+ L+R++             +  SK +LL  FK  GV L+ GD+ +HE+LVKAIKQVD VI T G  L+ DQVKII AIKEAGN+KRFFPSEFG DVDR  AV+P +  F  KA IRR VEAEG+PYTY+  + F  YFL NL+Q  AT PPRDKV+ILGDGN K  Y  E D+GTYTI+A +DPRTLNK+
Sbjct:    1 MASQNRILVLGPTGAIGRHVVWASIKAGNPTYALIRKTPGDINKPSLVAAANPESKEELLQSFKAAGVILLEGDMNDHEALVKAIKQVDTVICTFGRLLILDQVKIIKAIKEAGNVKRFFPSEFGLDVDRHDAVDPVRPVFDEKASIRRVVEAEGVPYTYLCCHAFTGYFLRNLAQFDATEPPRDKVIILGDGNVKGAYVTEADVGTYTIRAANDPRTLNKA 222          
BLAST of CX295642 vs. ExPASy Swiss-Prot
Match: IFR_PEA (Isoflavone reductase OS=Pisum sativum GN=IFR PE=2 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 1.034e-62
Identity = 124/222 (55.86%), Postives = 158/222 (71.17%), Query Frame = 1
Query:   67 MASKSKILFIGGTGYIGKFIVEASAKAGHPTFVLVREST-----------LSDPSKSQLLDHFKKLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEPAKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPKAVYNKEDDIGTYTIKAVDDPRTLNKS 699
            MA+++KIL +G TG IG+ IV AS KAG+PT+ LVR+++            +  +K +LL +++  GV L+ GD+ +HE+LV AIKQVD VI   G  L+ DQVK+I AIKEAGN+KRFFPSEFG DVDR  AVEP +  F  KA IRR VE+EG+PYTY+  + F  YFL NL+Q  AT PPRDKVVILGDGN +  Y  E D+GTYTI+A +DP TLNK+
Sbjct:    1 MATENKILILGATGAIGRHIVWASIKAGNPTYALVRKTSDNVNKPKLTEAANPETKEELLKNYQASGVILLEGDINDHETLVNAIKQVDTVICAAGRLLIEDQVKVIKAIKEAGNVKRFFPSEFGLDVDRHDAVEPVRQVFEEKASIRRVVESEGVPYTYLCCHAFTGYFLRNLAQIDATDPPRDKVVILGDGNVRGAYVTEADVGTYTIRAANDPNTLNKA 222          
BLAST of CX295642 vs. ExPASy Swiss-Prot
Match: IFRH_MAIZE (Isoflavone reductase homolog IRL OS=Zea mays GN=IRL PE=2 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 3.928e-62
Identity = 119/215 (55.35%), Postives = 149/215 (69.30%), Query Frame = 1
Query:   70 ASKSKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSDPSKSQLLDHFKKLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEPAKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPKAVYNKEDDIGTYTIKAVDDPRTLNKSFTLTP 714
            + KSKIL +GGTGY+G+ +V ASA+ GHPT  LVR++  SDP+K+ LL  F+  GV L+ GD+ +  SLV A+K  DVVIS +G   + DQ +++ AIKEAGN+KRFFPSEFG DVDR   VEPAKS    K  IRRA EA GIPYTY  + FFA + LP + Q  A  PP DK V+LGDG+ KAV+ +E DI TYT+ A DDPR  NK   + P
Sbjct:    3 SEKSKILVVGGTGYLGRHVVAASARLGHPTSALVRDTAPSDPAKAALLKSFQDAGVTLLKGDLYDQASLVSAVKGADVVISVLGSMQIADQSRLVDAIKEAGNVKRFFPSEFGLDVDRTGIVEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFAGFGLPKVGQVLAPGPPADKAVVLGDGDTKAVFVEEGDIATYTVLAADDPRAENKVLYIKP 217          
BLAST of CX295642 vs. ExPASy Swiss-Prot
Match: IFRH_LUPAL (Isoflavone reductase homolog OS=Lupinus albus PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 2.088e-55
Identity = 116/220 (52.73%), Postives = 147/220 (66.82%), Query Frame = 1
Query:   76 KSKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSDPSKSQLLDHFKKLGVNLVIGDVLNHESLVKAIKQVDVVISTVG------HTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRV-HAVEPAKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPKAVYNKEDDIGTYTIKAVDDPRTLNKSFTLTP 714
            KSK+L +GGTGY+G+ IV+AS + GH TF+L R     D  K Q+L  FKK G  LV     +H+SLV A+K VDVVI T+       H LL  Q+K++ AIK+AGNIKRF PSEFG D   + HA+EP +  F  K  +R+A+E   IP+TY+++N FA YF  NLSQ     PPRDKV++ GDGN K VY  EDD+ TYTIK +DDPRTLNK+  L P
Sbjct:    3 KSKVLVVGGTGYVGRRIVKASLEHGHETFILQRPEIGLDIEKLQILLSFKKQGAILVEASFSDHKSLVDAVKLVDVVICTMSGVHFRSHNLL-TQLKLVEAIKDAGNIKRFLPSEFGMDPALMGHALEPGRVTFDEKMTVRKAIEEANIPFTYISANCFAGYFAGNLSQMKTLLPPRDKVLLYGDGNVKPVYMDEDDVATYTIKTIDDPRTLNKTVYLRP 221          
BLAST of CX295642 vs. ExPASy Swiss-Prot
Match: IGS1_PETHY (Isoeugenol synthase 1 OS=Petunia hybrida GN=IGS1 PE=1 SV=1)

HSP 1 Score: 200.29 bits (508), Expect = 9.076e-51
Identity = 105/214 (49.07%), Postives = 139/214 (64.95%), Query Frame = 1
Query:   76 KSKILFIGGTGYIGKFIVEASAKAGHPTFVLVRE-STLSDPSKSQLLDHFKKLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEPAKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPKAVYNKEDDIGTYTIKAVDDPRTLNKSFTLTP 714
            K KIL +G TGY+GK++V+AS   GHPT+  V      SD SK QLL  F+ LGV +  G++  H+ LV   K+VD+VIST+      +Q+K+I AIKEAGNIKRF PSEFGN+VDRV A+   ++    K KIRRA EA GIP+T+V++N   AYF+  L  P   +   ++V I G G+ KAV N E+D+  YTIKA DDPR  N+   + P
Sbjct:    5 KGKILILGATGYLGKYMVKASISLGHPTYAYVMPLKKNSDDSKLQLLKEFESLGVTIFYGELSEHDKLVAVFKEVDIVISTLAVPQYLEQLKVIEAIKEAGNIKRFVPSEFGNEVDRVRALPRFQAVLDNKKKIRRATEAAGIPFTFVSANSLTAYFVDYLLHPRQKS---EQVTIYGSGDAKAVLNYEEDVAAYTIKAADDPRAANRVLIIKP 215          
BLAST of CX295642 vs. ExPASy Swiss-Prot
Match: EGS1_OCIBA (Eugenol synthase 1 OS=Ocimum basilicum GN=EGS1 PE=1 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 3.813e-49
Identity = 103/213 (48.36%), Postives = 137/213 (64.32%), Query Frame = 1
Query:   76 KSKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSDPSKSQLLDHFKKLGVNLVIGDVLNHESLVKAIKQVDVVISTVGHTLLGDQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVEPAKSAFATKAKIRRAVEAEGIPYTYVASNFFAAYFLPNLSQPGATAPPRDKVVILGDGNPKAVYNKEDDIGTYTIKAVDDPRTLNKSFTLTP 714
            KSKIL  GGTGYIG  +V+ S K GHPT+V  R ++    SK+ LLD F+ LG  +V G++  HE LV+ +K+VDVVIS +    + DQ KI+ AIK AGNIKRF PS+FG + DR++A+ P ++    K  IRRA+E   IPYTYV++N FA+YF+  L +P     P+D++ + G G  K   N E DIG YTIK   DPR LN+     P
Sbjct:    7 KSKILIFGGTGYIGNHMVKGSLKLGHPTYVFTRPNS----SKTTLLDEFQSLGAIIVKGELDEHEKLVELMKKVDVVISALAFPQILDQFKILEAIKVAGNIKRFLPSDFGVEEDRINALPPFEALIERKRMIRRAIEEANIPYTYVSANCFASYFINYLLRP---YDPKDEITVYGTGEAKFAMNYEQDIGLYTIKVATDPRALNRVVIYRP 212          
The following BLAST results are available for this feature:
BLAST of CX295642 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11
Match NameE-valueIdentityDescription
IFRH_SOLTU6.638e-9479.63Isoflavone reductase homolog OS=Solanum tuberosum ... [more]
IFRH_TOBAC7.613e-8269.59Isoflavone reductase homolog A622 OS=Nicotiana tab... [more]
IFRH_ARATH9.306e-8071.16Isoflavone reductase homolog P3 OS=Arabidopsis tha... [more]
IFR_MEDSA1.104e-6457.21Isoflavone reductase OS=Medicago sativa GN=IFR PE=... [more]
IFR_CICAR1.884e-6458.11Isoflavone reductase OS=Cicer arietinum GN=IFR PE=... [more]
IFR_PEA1.034e-6255.86Isoflavone reductase OS=Pisum sativum GN=IFR PE=2 ... [more]
IFRH_MAIZE3.928e-6255.35Isoflavone reductase homolog IRL OS=Zea mays GN=IR... [more]
IFRH_LUPAL2.088e-5552.73Isoflavone reductase homolog OS=Lupinus albus PE=2... [more]
IGS1_PETHY9.076e-5149.07Isoeugenol synthase 1 OS=Petunia hybrida GN=IGS1 P... [more]
EGS1_OCIBA3.813e-4948.36Eugenol synthase 1 OS=Ocimum basilicum GN=EGS1 PE=... [more]

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Properties
Property NameValue
Genbank descriptionC05074D03SK DroRLeaf1 Citrus clementina cDNA clone C05074D03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295642 ID=CX295642; Name=CX295642; organism=Citrus clementina; type=EST; length=736bp
TTCGGCCGAGGACAAACAGAAAAAAAAGGAAAGAATCCCCGAAATTGAAC
TGAATAATTCTCGGTAATGGCGTCGAAAAGCAAGATTCTATTCATCGGCG
GTACAGGGTACATCGGCAAATTCATCGTCGAAGCGAGCGCGAAAGCCGGC
CACCCGACGTTCGTGCTCGTGAGAGAATCCACACTCTCCGATCCTTCCAA
ATCTCAGCTCCTCGATCACTTCAAAAAACTCGGCGTCAATCTCGTCATCG
GAGATGTGTTGAATCACGAGAGCTTGGTGAAGGCGATAAAACAAGTGGAC
GTGGTGATATCAACGGTCGGTCACACTTTATTAGGTGATCAGGTCAAGAT
TATTGCGGCCATTAAAGAAGCTGGTAACATTAAGAGATTCTTCCCTTCAG
AATTTGGGAATGATGTTGATCGAGTCCATGCCGTTGAGCCAGCAAAATCA
GCATTTGCCACAAAGGCAAAAATCCGCCGAGCCGTTGAGGCTGAAGGGAT
TCCGTATACTTATGTAGCGTCGAATTTCTTTGCTGCTTACTTCCTTCCCA
ATTTGTCGCAGCCCGGGGCCACTGCCCCACCTAGGGATAAAGTTGTCATC
TTAGGCGATGGAAATCCAAAAGCTGTTTATAACAAGGAAGATGATATTGG
AACCTATACTATCAAAGCGGTTGATGATCCCAGAACCTTGAACAAATCCT
TTACATTAACCCCCTGGTATATTACTCATTCATGAT
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