DY262819

Overview
NameDY262819
Unique NameDY262819
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length787
Libraries
Library NameType
CitNFLcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM_RAT (Malate dehydrogenase, mitochondrial OS=Rattus norvegicus GN=Mdh2 PE=1 SV=2)

HSP 1 Score: 105.145 bits (261), Expect = 4.338e-25
Identity = 55/108 (50.93%), Postives = 66/108 (61.11%), Query Frame = 2 
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNG 328
            YN  K+FGVTTLD+VRA TF                   HAG TI+PL SQ TPK +   + +  LT R Q+ GTEVV+AKAG GSATLSMAYAGA F  + +  +NG
Sbjct:  161 YNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLATLTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNG 268

HSP 2 Score: 31.9574 bits (71), Expect = 4.338e-25
Identity = 22/61 (36.07%), Postives = 35/61 (57.38%), Query Frame = 3 
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGVGKK*NPKLKAFHLKRG 524
            +E SFVQS  TE  +F++ + LGK+G  K   + G +     K + +   P+LKA  +K+G
Sbjct:  273 IECSFVQSKETECTYFSTPLLLGKKGLEKNLGI-GKITPFEEKMIAEA-IPELKA-SIKKG 330
[Close]
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM_PIG (Malate dehydrogenase, mitochondrial OS=Sus scrofa GN=MDH2 PE=1 SV=2)

HSP 1 Score: 103.99 bits (258), Expect = 5.646e-25
Identity = 57/119 (47.90%), Postives = 70/119 (58.82%), Query Frame = 2 
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDVGGMVICT 361
            YN  K+FGVTTLD+VRA  F                   HAG TI+PL SQ TPK +   + +  LT R Q+ GTEVV+AKAG GSATLSMAYAGA F  + +  +NG     G+V C+
Sbjct:  161 YNPNKIFGVTTLDIVRANAFVAELKGLDPARVSVPVIGGHAGKTIIPLISQCTPKVDFPQDQLSTLTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKE---GVVECS 276

HSP 2 Score: 32.7278 bits (73), Expect = 5.646e-25
Identity = 22/61 (36.07%), Postives = 36/61 (59.02%), Query Frame = 3 
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGVGKK*NPKLKAFHLKRG 524
            VE SFV+S  T+ P+F++ + LGK+G  K   + G +     K + +   P+LKA  +K+G
Sbjct:  273 VECSFVKSQETDCPYFSTPLLLGKKGIEKNLGI-GKISPFEEKMIAEA-IPELKA-SIKKG 330
[Close]
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM_BOVIN (Malate dehydrogenase, mitochondrial OS=Bos taurus GN=MDH2 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.244e-24
Identity = 57/119 (47.90%), Postives = 69/119 (57.98%), Query Frame = 2 
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDVGGMVICT 361
            YN  K+FGVTTLD+VRA  F                   HAG TI+PL SQ TPK     + +  LT R Q+ GTEVV+AKAG GSATLSMAYAGA F  + +  +NG     G+V C+
Sbjct:  161 YNPNKIFGVTTLDIVRANAFVAELKDLDPARVNVPVIGGHAGKTIIPLISQCTPKVEFPQDQLTTLTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKE---GVVECS 276

HSP 2 Score: 32.3426 bits (72), Expect = 1.244e-24
Identity = 22/61 (36.07%), Postives = 36/61 (59.02%), Query Frame = 3 
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGVGKK*NPKLKAFHLKRG 524
            VE SFV+S  T+ P+F++ + LGK+G  K   + G +     K + +   P+LKA  +K+G
Sbjct:  273 VECSFVKSQETDCPYFSTPLLLGKKGIEKNLGI-GKVSPFEEKMIAEA-IPELKA-SIKKG 330
[Close]
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM_CAEEL (Probable malate dehydrogenase, mitochondrial OS=Caenorhabditis elegans GN=mdh-1 PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 5.245e-23
Identity = 57/112 (50.89%), Postives = 71/112 (63.39%), Query Frame = 2 
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDV 340
            Y+ K++FGVTTLDVVR++ F                   HAGITI+PL SQ  P    ++E+I  LT R QD GTEVV AKAG GSATLSMA AGA FA+A ++G+ G  +V
Sbjct:  165 YDPKRVFGVTTLDVVRSQAFVSELKGHDASKTVVPVVGGHAGITIIPLLSQVKPSTKFSEEEISKLTPRIQDAGTEVVNAKAGAGSATLSMALAGARFANALVRGIKGEKNV 276
[Close]
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDH_VIBCH (Malate dehydrogenase OS=Vibrio cholerae GN=mdh PE=3 SV=2)

HSP 1 Score: 100.138 bits (248), Expect = 1.857e-22
Identity = 56/112 (50.00%), Postives = 69/112 (61.61%), Query Frame = 2 
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDV 340
            Y+++KLFGVTTLDV+R++TF                   H+G+TILPL SQ     +  DE++ ALTKR Q+ GTEVVEAKAG GSATLSM  A   F  A +K L G  DV
Sbjct:  138 YDKRKLFGVTTLDVIRSETFVAALKDKDPGQVRVPVIGGHSGVTILPLLSQVEG-VSFTDEEVAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVKALQGESDV 248

HSP 2 Score: 28.1054 bits (61), Expect = 1.857e-22
Identity = 11/26 (42.31%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEG 419
            VE+++V+      PFFA  ++LGK G
Sbjct:  249 VEYAYVEGEGEYAPFFAQPIKLGKNG 274
[Close]
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDH_PASMU (Malate dehydrogenase OS=Pasteurella multocida GN=mdh PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 3.143e-22
Identity = 57/108 (52.78%), Postives = 70/108 (64.81%), Query Frame = 2 
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNG 328
            Y+++KLFGVTTLD++R++TF                   H+G+TILPL SQ    A   D +I  LTKR Q+ GTEVVEAKAG GSATLSMA A A FA + +KGLNG
Sbjct:  138 YDKRKLFGVTTLDIIRSETFVSELKNLEPARTTVPVIGGHSGVTILPLLSQVQ-YAEWNDAEIAPLTKRIQNAGTEVVEAKAGGGSATLSMAQAAARFALSLVKGLNG 244

HSP 2 Score: 26.5646 bits (57), Expect = 3.143e-22
Identity = 13/26 (50.00%), Postives = 16/26 (61.54%), Query Frame = 3 
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEG 419
            VE ++V+       FFA  VRLGKEG
Sbjct:  248 VECTYVEGDGKYARFFAQPVRLGKEG 273
[Close]
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDH_HAEPS (Malate dehydrogenase OS=Haemophilus parasuis serovar 5 (strain SH0165) GN=mdh PE=3 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 5.319e-22
Identity = 58/110 (52.73%), Postives = 72/110 (65.45%), Query Frame = 2 
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQA--TPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNG 328
            Y+++KLFGVT+LDV+R++TF                   H+G+TILPL SQA    K +   E++ ALTKR Q+ GTEVVEAKAG GSATLSMA A A FA + LKGL G
Sbjct:  138 YDKRKLFGVTSLDVLRSETFVAELKGKDVNDVKVPVIGGHSGVTILPLLSQAFEEDKIDFTAEEVAALTKRIQNAGTEVVEAKAGGGSATLSMAQAAARFARSVLKGLTG 247

HSP 2 Score: 23.8682 bits (50), Expect = 5.319e-22
Identity = 11/26 (42.31%), Postives = 15/26 (57.69%), Query Frame = 3 
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEG 419
            VE+++V+       FFA  VRLG  G
Sbjct:  251 VEYAYVEGNGEYARFFAQPVRLGLNG 276
[Close]
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDH_PROMH (Malate dehydrogenase OS=Proteus mirabilis (strain HI4320) GN=mdh PE=3 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 6.920e-22
Identity = 56/112 (50.00%), Postives = 70/112 (62.50%), Query Frame = 2 
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDV 340
            Y++K+LFGVTTLD++RA TF                   H+G+TILPL SQ     +  DE++ ALTKR Q+ GTEVVEAKAG GSATLSM  A A F  + ++ LNG  DV
Sbjct:  138 YDKKRLFGVTTLDIIRANTFVAELKGKDPQTTNVPVIGGHSGVTILPLLSQVAG-VSFTDEEVAALTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLIRALNGEKDV 248

HSP 2 Score: 23.483 bits (49), Expect = 6.920e-22
Identity = 10/26 (38.46%), Postives = 14/26 (53.85%), Query Frame = 3 
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEG 419
            +E ++ +       FFA  VRLGK G
Sbjct:  249 IECTYTEGDGEHARFFAQPVRLGKNG 274
[Close]
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDH_VIBVY (Malate dehydrogenase OS=Vibrio vulnificus (strain YJ016) GN=mdh PE=3 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 9.005e-22
Identity = 55/108 (50.93%), Postives = 67/108 (62.04%), Query Frame = 2 
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNG 328
            Y+++KLFGVTTLDV+R++TF                   H+G+TILPL SQ       +DE+I ALTKR Q+ GTEVVEAKAG GSATLSM  A   F  A +K L G
Sbjct:  138 YDKRKLFGVTTLDVIRSETFVAELKGQDPGEVRVPVIGGHSGVTILPLLSQVEG-VEFSDEEIAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVKALQG 244

HSP 2 Score: 28.1054 bits (61), Expect = 9.005e-22
Identity = 11/26 (42.31%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEG 419
            +E+++V+       FFA  V+LGKEG
Sbjct:  248 IEYAYVEGNGEHASFFAQPVKLGKEG 273
[Close]
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDH_VIBVU (Malate dehydrogenase OS=Vibrio vulnificus GN=mdh PE=3 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 9.005e-22
Identity = 55/108 (50.93%), Postives = 67/108 (62.04%), Query Frame = 2 
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNG 328
            Y+++KLFGVTTLDV+R++TF                   H+G+TILPL SQ       +DE+I ALTKR Q+ GTEVVEAKAG GSATLSM  A   F  A +K L G
Sbjct:  138 YDKRKLFGVTTLDVIRSETFVAELKGQDPGEVRVPVIGGHSGVTILPLLSQVEG-VEFSDEEIAALTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVKALQG 244

HSP 2 Score: 28.1054 bits (61), Expect = 9.005e-22
Identity = 11/26 (42.31%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEG 419
            +E+++V+       FFA  V+LGKEG
Sbjct:  248 IEYAYVEGNGEHASFFAQPVKLGKEG 273
The following BLAST results are available for this feature:
BLAST of DY262819 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 153
Match NameE-valueIdentityDescription
MDHM_RAT4.338e-2550.93Malate dehydrogenase, mitochondrial OS=Rattus norv... [more]
MDHM_PIG5.646e-2547.90Malate dehydrogenase, mitochondrial OS=Sus scrofa ... [more]
MDHM_BOVIN1.244e-2447.90Malate dehydrogenase, mitochondrial OS=Bos taurus ... [more]
MDHM_CAEEL5.245e-2350.89Probable malate dehydrogenase, mitochondrial OS=Ca... [more]
MDH_VIBCH1.857e-2250.00Malate dehydrogenase OS=Vibrio cholerae GN=mdh PE=... [more]
MDH_PASMU3.143e-2252.78Malate dehydrogenase OS=Pasteurella multocida GN=m... [more]
MDH_HAEPS5.319e-2252.73Malate dehydrogenase OS=Haemophilus parasuis serov... [more]
MDH_PROMH6.920e-2250.00Malate dehydrogenase OS=Proteus mirabilis (strain ... [more]
MDH_VIBVY9.005e-2250.93Malate dehydrogenase OS=Vibrio vulnificus (strain ... [more]
MDH_VIBVU9.005e-2250.93Malate dehydrogenase OS=Vibrio vulnificus GN=mdh P... [more]

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Properties
Property NameValue
Genbank descriptionIC0AAA10AB09RM1 CitNFL Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY262819 ID=DY262819; Name=DY262819; organism=Citrus clementina; type=EST; length=787bp
GACATACAATGAGAAGAAGCTGTTTGGTGTGACCACTCTTGATGTGGTTA
GGGCTAAGACTTTCTATGCTGGAAAGGCCAATGTTAATGTTGCAGAGGTT
AATGTGCCTGTTGTTGGTGGTCATGCTGGTATAACAATTCTCCCGCTATT
TTCTCAGGCCACACCAAAGGCCAATTTGGCAGATGAAGATATTAAGGCTC
TTACAAAGAGAACCCAAGATGGTGGGACAGAAGTTGTGGAAGCAAAGGCT
GGAAAGGGTTCTGCAACATTGTCAATGGCTTATGCAGGAGCCATTTTTGC
TGATGCATGCCTGAAGGGTCTTAATGGTGTTCCAGATGTGGGTGGAATGG
TCATTTGTACAGTCAACTGTCACTGAGCTTCCCTTTTTTGCTTCAAAGGT
GAGGCTCGGAAAAGAAGGGGGTAGAAAAAAGTTCTGGGTTTGGGGTCCTC
TCCCCGAACTTCCAACAAAAGGGGTTGGAAAAAAATGAAACCCAAAACTC
AAAGCCTTCCATTTAAAAAGGGGAGTCAGTTTGGCCATGGCCAATTTAGC
CCACCCTTCCCAAAAAAAAAAATGGGTTCAAATTTTGGCAATTTTTTTTC
CCCAGGTGGTTAAATTTGTTGGCCCCAAGGGCCCCAGCCTTTTTTTGGGG
GGGTTTTTTCTTTTTTAAAAAGAGGCTTTTTTTAAAAAAAACCCAAAAAA
TTTTCTCCCAATAAATATAATATTCTCTTGTGGAAAAGGGGGTTTTAAGG
CGGGGGGGGGGAGGACAATTTTTTAAAAAAAGAAAAT
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