DY262819

Overview
NameDY262819
Unique NameDY262819
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length787
Libraries
Library NameType
CitNFLcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM_EUCGU (Malate dehydrogenase, mitochondrial OS=Eucalyptus gunnii GN=MDH PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 3.085e-50
Identity = 92/113 (81.42%), Postives = 93/113 (82.30%), Query Frame = 2
Query:    2 TYNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDV 340
            TYNEKKL GVTTLDVVRAKTFY                  HAGITILPLFSQA PKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGA+FADACLKGLNGVPDV
Sbjct:  170 TYNEKKLLGVTTLDVVRAKTFYAGKAKVPVEEVNVPVVGGHAGITILPLFSQAVPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADACLKGLNGVPDV 282          

HSP 2 Score: 44.2838 bits (103), Expect = 3.085e-50
Identity = 30/66 (45.45%), Postives = 45/66 (68.18%), Query Frame = 3
Query:  342 VEWSFVQSTV-TELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGVGKK*NPKLKAFHLKRGVSLA 536
            VE SFVQS++ TELPFFASKV+LGK G  +   + GP+ +   +G+ +   P+LKA  +++G+  A
Sbjct:  283 VECSFVQSSIITELPFFASKVKLGKNGVEEVLEL-GPMSDYEKQGL-EILIPELKA-SIEKGIKFA 345          
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM2_ARATH (Malate dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana GN=At3g15020 PE=1 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 9.672e-49
Identity = 85/113 (75.22%), Postives = 94/113 (83.19%), Query Frame = 2
Query:    2 TYNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDV 340
            TY+EKKLFGVTTLDVVRA+TFY                  HAGITILPLFSQA+P+ANL+D+ I+ALTKRTQDGGTEVVEAKAGKGSATLSMAYAGA+FADACLKGLNGVP+V
Sbjct:  165 TYDEKKLFGVTTLDVVRARTFYAGKSDVNVAEVNVPVVGGHAGITILPLFSQASPQANLSDDLIRALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADACLKGLNGVPNV 277          

HSP 2 Score: 50.447 bits (119), Expect = 9.672e-49
Identity = 27/45 (60.00%), Postives = 33/45 (73.33%), Query Frame = 3
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGV 476
            VE SFVQST+TELPFFASKVRLGK G  +   + GPL +   +G+
Sbjct:  278 VECSFVQSTITELPFFASKVRLGKNGVEEVLDL-GPLSDFEKEGL 321          
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM1_ARATH (Malate dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana GN=At1g53240 PE=1 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 2.141e-48
Identity = 83/112 (74.11%), Postives = 91/112 (81.25%), Query Frame = 2
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDV 340
            Y+EKKLFGVTTLDVVRA+TFY                  HAG+TILPLFSQATP+ANL+ + + ALTKRTQDGGTEVVEAKAGKGSATLSMAYAGA+FADACLKGLNGVPDV
Sbjct:  166 YDEKKLFGVTTLDVVRARTFYAGKANVPVAEVNVPVIGGHAGVTILPLFSQATPQANLSSDILTALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADACLKGLNGVPDV 277          

HSP 2 Score: 51.9878 bits (123), Expect = 2.141e-48
Identity = 31/65 (47.69%), Postives = 45/65 (69.23%), Query Frame = 3
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGVGKK*NPKLKAFHLKRGVSLA 536
            +E S+VQST+TELPFFASKVRLGK G  +   + GPL +   +G+ +   P+LK+  +++GV  A
Sbjct:  278 IECSYVQSTITELPFFASKVRLGKNGVEEVLDL-GPLSDFEKEGL-EALKPELKS-SIEKGVKFA 339          
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM_BRANA (Malate dehydrogenase, mitochondrial OS=Brassica napus GN=MDH PE=2 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 5.035e-44
Identity = 78/112 (69.64%), Postives = 85/112 (75.89%), Query Frame = 2
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDV 340
            Y+EKKLFGVTTLDVVR KT Y                  HAG+TILPLFSQATP+A L+ + +   TKRTQDGGTEV EAKAGKGSATLSMAYAGA+FADACLKGLNGVPDV
Sbjct:  166 YDEKKLFGVTTLDVVRVKTSYAGKANVPVAEVNVPAIVGHAGVTILPLFSQATPQAILSGDALTVTTKRTQDGGTEVEEAKAGKGSATLSMAYAGALFADACLKGLNGVPDV 277          

HSP 2 Score: 50.0618 bits (118), Expect = 5.035e-44
Identity = 31/65 (47.69%), Postives = 44/65 (67.69%), Query Frame = 3
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGVGKK*NPKLKAFHLKRGVSLA 536
            VE S+VQST+TELPFFASKVRLGK G  +   + GPL +   +G+ +   P +K+  +++GV  A
Sbjct:  278 VECSYVQSTITELPFFASKVRLGKNGVEEVLDL-GPLSDFEKEGL-EALRPGIKS-TIEKGVKFA 339          
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM_CITLA (Malate dehydrogenase, mitochondrial OS=Citrullus lanatus GN=MMDH PE=1 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 2.958e-42
Identity = 103/179 (57.54%), Postives = 116/179 (64.80%), Query Frame = 2
Query:    2 TYNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDVGGMVICTVNCH*ASLFCFKGEAXXXXXXXXVLGLGSSPRTSNKRGWKKMKPKTQSLPFKKGSQFGH 538
            TY+EKKLFGVTTLDVVRAKTFY                  HAGITILPLFSQATP+ANL+D+ I ALTKRTQDGGTEVVEAKAGKGSATLSMAYAGA+FADACLKGLNGVPDV               F  K +         VL LG       K G +K+KP+ ++   +KG QF +
Sbjct:  170 TYDEKKLFGVTTLDVVRAKTFYAGKANVPVAEVNVPVIGGHAGITILPLFSQATPRANLSDDTIVALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADACLKGLNGVPDVVECSFVQSTVTELPFFASKVK-LGKNGVESVLDLGPL-SDFEKEGLEKLKPELKA-SIEKGIQFAN 345          
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM_FRAAN (Malate dehydrogenase, mitochondrial OS=Fragaria ananassa GN=MMDHI PE=1 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 6.589e-42
Identity = 106/180 (58.89%), Postives = 119/180 (66.11%), Query Frame = 2
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDVGGMVICT-VNCH*ASLFCFKGEA-XXXXXXXXVLGLGSSPRTSNKRGWKKMKPKTQSLPFKKGSQFGH 538
            Y+EKKLFGVTTLDVVRAKTFY                  HAGITILPLFSQATPKANL+D+ IKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGA+FADACL GLNGVPDV   V C+ V      L  F  +          VL LG       K G K++KP+ +S   +KG +F +
Sbjct:  163 YDEKKLFGVTTLDVVRAKTFYAGKAGVPVAEVNVPVVGGHAGITILPLFSQATPKANLSDDYIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGALFADACLXGLNGVPDV---VECSYVQSSITELPFFASKVRLGKNGVEEVLDLGPL-SDFEKEGLKQLKPELKS-SIEKGIKFAN 337          
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHM_CHLRE (Malate dehydrogenase, mitochondrial OS=Chlamydomonas reinhardtii PE=3 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 1.529e-39
Identity = 74/112 (66.07%), Postives = 86/112 (76.79%), Query Frame = 2
Query:    5 YNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDV 340
            Y+++K+ GVTTLDVVRAKTFY                  HAG+TILPLFSQATPKA ++ E + ALTKRTQDGGTEVV+AKAGKGSATLSMAYA A+FAD+CL+GLNG P V
Sbjct:  198 YDKRKVMGVTTLDVVRAKTFYAEKNGLDVASVDVPVVGGHAGVTILPLFSQATPKATMSAEVLDALTKRTQDGGTEVVQAKAGKGSATLSMAYAAALFADSCLRGLNGAPVV 309          

HSP 2 Score: 39.2762 bits (90), Expect = 1.529e-39
Identity = 27/62 (43.55%), Postives = 39/62 (62.90%), Query Frame = 3
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGVGKK*NPKLKAFHLKRGV 527
            VE ++V+STVT+ P+FASKV+L  E G  K    GPL +    G+ K   P+L A  +++GV
Sbjct:  309 VECTYVESTVTDAPYFASKVKLSTE-GVDKIHDLGPLSDYEKAGL-KAMMPELLA-SIEKGV 367          
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHG_ORYSJ (Malate dehydrogenase, glyoxysomal OS=Oryza sativa subsp. japonica GN=Os12g0632700 PE=1 SV=3)

HSP 1 Score: 120.168 bits (300), Expect = 1.432e-33
Identity = 66/120 (55.00%), Postives = 76/120 (63.33%), Query Frame = 2
Query:    2 TYNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDVGGMVICT 361
            TY+ K+L GVTTLDVVRA TF                   HAG+TILPL SQ  P  +   E+I  LT R Q+GGTEVVEAKAG GSATLSMAYA + FADACL+GL G     G+V C+
Sbjct:  180 TYDPKRLLGVTTLDVVRANTFVAEVLGLDPRDVNVPVIGGHAGVTILPLLSQVNPPCSFTSEEISYLTTRIQNGGTEVVEAKAGAGSATLSMAYAASKFADACLRGLRG---DAGIVECS 296          

HSP 2 Score: 45.4394 bits (106), Expect = 1.432e-33
Identity = 31/63 (49.21%), Postives = 39/63 (61.90%), Query Frame = 3
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGVGKK*NPKLKAFHLKRGVS 530
            VE SFV S VTELPFFASKVRLG+  G ++    GPL E    G+ K    K  A  +++GV+
Sbjct:  293 VECSFVASQVTELPFFASKVRLGR-CGIEEILSLGPLNEFERAGLEKA--KKELAESIQKGVA 352          
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHG2_BRANA (Malate dehydrogenase 2, glyoxysomal OS=Brassica napus GN=MDH2 PE=3 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 4.120e-33
Identity = 64/113 (56.64%), Postives = 73/113 (64.60%), Query Frame = 2
Query:    2 TYNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDV 340
            TY+ KKL GVTTLDV RA TF                   HAG+TILPL SQ  P ++    +I+ LT R Q+GGTEVVEAKAG GSATLSMAYA A FADACL+GL G  +V
Sbjct:  182 TYDPKKLLGVTTLDVARANTFVAEVLGLDPREVDVPVVGGHAGVTILPLLSQVKPPSSFTPSEIEYLTNRIQNGGTEVVEAKAGAGSATLSMAYAAAKFADACLRGLRGDANV 294          

HSP 2 Score: 44.669 bits (104), Expect = 4.120e-33
Identity = 25/47 (53.19%), Postives = 31/47 (65.96%), Query Frame = 3
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGVGK 482
            +E SFV S VTEL FFA+KVRLG+ G  + F + GPL E    G+ K
Sbjct:  295 IECSFVASQVTELAFFATKVRLGRTGAEEVFQL-GPLNEYERVGLEK 340          
BLAST of DY262819 vs. ExPASy Swiss-Prot
Match: MDHG1_BRANA (Malate dehydrogenase 1, glyoxysomal OS=Brassica napus GN=MDH1 PE=2 SV=2)

HSP 1 Score: 119.398 bits (298), Expect = 4.120e-33
Identity = 64/113 (56.64%), Postives = 73/113 (64.60%), Query Frame = 2
Query:    2 TYNEKKLFGVTTLDVVRAKTFYXXXXXXXXXXXXXXXXXXHAGITILPLFSQATPKANLADEDIKALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDV 340
            TY+ KKL GVTTLDV RA TF                   HAG+TILPL SQ  P ++    +I+ LT R Q+GGTEVVEAKAG GSATLSMAYA A FADACL+GL G  +V
Sbjct:  182 TYDPKKLLGVTTLDVARANTFVAEVLGLDPREVDVPVVGGHAGVTILPLLSQVKPPSSFTPSEIEYLTNRIQNGGTEVVEAKAGAGSATLSMAYAAAKFADACLRGLRGDANV 294          

HSP 2 Score: 44.669 bits (104), Expect = 4.120e-33
Identity = 25/47 (53.19%), Postives = 31/47 (65.96%), Query Frame = 3
Query:  342 VEWSFVQSTVTELPFFASKVRLGKEGGRKKFWVWGPLPELPTKGVGK 482
            +E SFV S VTEL FFA+KVRLG+ G  + F + GPL E    G+ K
Sbjct:  295 IECSFVASQVTELAFFATKVRLGRTGAEEVFQL-GPLNEYERVGLEK 340          
The following BLAST results are available for this feature:
BLAST of DY262819 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 153
Match NameE-valueIdentityDescription
MDHM_EUCGU3.085e-5081.42Malate dehydrogenase, mitochondrial OS=Eucalyptus ... [more]
MDHM2_ARATH9.672e-4975.22Malate dehydrogenase 2, mitochondrial OS=Arabidops... [more]
MDHM1_ARATH2.141e-4874.11Malate dehydrogenase 1, mitochondrial OS=Arabidops... [more]
MDHM_BRANA5.035e-4469.64Malate dehydrogenase, mitochondrial OS=Brassica na... [more]
MDHM_CITLA2.958e-4257.54Malate dehydrogenase, mitochondrial OS=Citrullus l... [more]
MDHM_FRAAN6.589e-4258.89Malate dehydrogenase, mitochondrial OS=Fragaria an... [more]
MDHM_CHLRE1.529e-3966.07Malate dehydrogenase, mitochondrial OS=Chlamydomon... [more]
MDHG_ORYSJ1.432e-3355.00Malate dehydrogenase, glyoxysomal OS=Oryza sativa ... [more]
MDHG2_BRANA4.120e-3356.64Malate dehydrogenase 2, glyoxysomal OS=Brassica na... [more]
MDHG1_BRANA4.120e-3356.64Malate dehydrogenase 1, glyoxysomal OS=Brassica na... [more]

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Properties
Property NameValue
Genbank descriptionIC0AAA10AB09RM1 CitNFL Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY262819 ID=DY262819; Name=DY262819; organism=Citrus clementina; type=EST; length=787bp
GACATACAATGAGAAGAAGCTGTTTGGTGTGACCACTCTTGATGTGGTTA
GGGCTAAGACTTTCTATGCTGGAAAGGCCAATGTTAATGTTGCAGAGGTT
AATGTGCCTGTTGTTGGTGGTCATGCTGGTATAACAATTCTCCCGCTATT
TTCTCAGGCCACACCAAAGGCCAATTTGGCAGATGAAGATATTAAGGCTC
TTACAAAGAGAACCCAAGATGGTGGGACAGAAGTTGTGGAAGCAAAGGCT
GGAAAGGGTTCTGCAACATTGTCAATGGCTTATGCAGGAGCCATTTTTGC
TGATGCATGCCTGAAGGGTCTTAATGGTGTTCCAGATGTGGGTGGAATGG
TCATTTGTACAGTCAACTGTCACTGAGCTTCCCTTTTTTGCTTCAAAGGT
GAGGCTCGGAAAAGAAGGGGGTAGAAAAAAGTTCTGGGTTTGGGGTCCTC
TCCCCGAACTTCCAACAAAAGGGGTTGGAAAAAAATGAAACCCAAAACTC
AAAGCCTTCCATTTAAAAAGGGGAGTCAGTTTGGCCATGGCCAATTTAGC
CCACCCTTCCCAAAAAAAAAAATGGGTTCAAATTTTGGCAATTTTTTTTC
CCCAGGTGGTTAAATTTGTTGGCCCCAAGGGCCCCAGCCTTTTTTTGGGG
GGGTTTTTTCTTTTTTAAAAAGAGGCTTTTTTTAAAAAAAACCCAAAAAA
TTTTCTCCCAATAAATATAATATTCTCTTGTGGAAAAGGGGGTTTTAAGG
CGGGGGGGGGGAGGACAATTTTTTAAAAAAAGAAAAT
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