FC875689

Overview
NameFC875689
Unique NameFC875689
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length712
Libraries
Library NameType
CTVClemen1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig14122 contig Ccv1_Contig14122:46..758. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_ORYSJ (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica GN=GLX-I PE=1 SV=2)

HSP 1 Score: 354.369 bits (908), Expect = 3.546e-97
Identity = 169/205 (82.44%), Postives = 186/205 (90.73%), Query Frame = 3 
Query:   90 WPKKDKRRLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGG-NVTREPGPVKGGTTHIAFVKDPDGYIFELIQRGPTPEPLCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTR 701
            WPKKDK+RLLHAVYRVGDLDRTIK YTECFGMKLLRKRDVPEEKY+NAFLGFGPE ++F +ELTYNYGV  YDIG GFGHFAIATEDVYKL E I++     +TREPGPVKGG+T IAF +DPDGY+FELIQRGPTPEPLCQVMLRVGDL RSIKFYEKALGMKLLR  D P+YKYT+AMLGYA+ED+TTV+ELTYNYGVTEYT+
Sbjct:   17 WPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTK 221

HSP 2 Score: 125.946 bits (315), Expect = 2.051e-28
Identity = 64/126 (50.79%), Postives = 80/126 (63.49%), Query Frame = 3 
Query:  114 LLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENI----RAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELI 479
            L   + RVGDLDR+IKFY +  GMKLLRK+DVP+ KY+ A LG+  E    V+ELTYNYGVT Y  G  +   AI TEDVYK  E +    +  GG + R+PGP+ G  T IA   DPDG+   L+
Sbjct:  156 LCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGKILRQPGPLPGLNTKIASFLDPDGWKVVLV 281
[Close]
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_BRAOG (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera PE=2 SV=1)

HSP 1 Score: 354.369 bits (908), Expect = 3.546e-97
Identity = 168/204 (82.35%), Postives = 188/204 (92.16%), Query Frame = 3 
Query:   90 WPKKDKRRLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQRGPTPEPLCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTR 701
            WPKKDKRR LH VYRVGDLDRTI+FYTECFGMK+LRKRDVPEEKYSNAFLGFGPE S+FVVELTYNYGV+SYDIGTGFGHFAI+T+DV K+VE +RAKGGNVTREPGPVKGG + IAFVKDPDGY FELIQRGPTPEPLCQVMLRVGDL R++KF EKALGM+LLR +++PEY  T+ M+GYAEE ++ VLELTYNYGVTEYT+
Sbjct:   10 WPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTK 212

HSP 2 Score: 99.7525 bits (247), Expect = 1.574e-20
Identity = 53/121 (43.80%), Postives = 70/121 (57.85%), Query Frame = 3 
Query:  114 LLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIR----AKGGNVTREPGPVKGGTTHIAFVKDPDGY 464
            L   + RVGDLDR +KF  +  GM+LLR+ + PE   +   +G+  E    V+ELTYNYGVT Y  G  +   AI T+DVYK  E ++      GG +TRE GP+ G  T I    DPDG+
Sbjct:  148 LCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGW 267
[Close]
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_VIBPA (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus GN=gloA PE=3 SV=2)

HSP 1 Score: 168.703 bits (426), Expect = 2.759e-41
Identity = 78/124 (62.90%), Postives = 96/124 (77.42%), Query Frame = 3 
Query:  111 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 482
            R+LH + RVGDLD++IKFYTE  GM+LLR  +  E +Y+ AF+G+G E    V+ELTYN+G T YD+GT FGH AI  +D+Y   + I+A GGNVTRE GPVKGGTTHIAFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEYDLGTAFGHIAIGVDDIYATCDAIKAAGGNVTREAGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 83.5741 bits (205), Expect = 1.167e-15
Identity = 38/64 (59.38%), Postives = 49/64 (76.56%), Query Frame = 3 
Query:  504 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEY 695
            +   MLRVGDL +SIKFY + +GM+LLRT +  EY+YTLA +GY +E Q  V+ELTYN+G TEY
Sbjct:    6 ILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEY 69
[Close]
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_VIBCH (Probable lactoylglutathione lyase OS=Vibrio cholerae GN=gloA PE=3 SV=2)

HSP 1 Score: 164.851 bits (416), Expect = 3.984e-40
Identity = 76/124 (61.29%), Postives = 94/124 (75.81%), Query Frame = 3 
Query:  111 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 482
            R+LH + RVGDLD++I+FYT+  GM LLRK +  E KY+ AFLG+G E    V+ELTYN+GV  Y+ G  +GH AI  +D+Y   + I+A GG VTREPGPVKGGTTHIAFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 81.6481 bits (200), Expect = 4.436e-15
Identity = 37/66 (56.06%), Postives = 48/66 (72.73%), Query Frame = 3 
Query:  504 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEYTR 701
            +   MLRVGDL +SI+FY + +GM LLR  +  EYKYTLA LGY +E Q  V+ELTYN+GV +Y +
Sbjct:    6 ILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEK 71
[Close]
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_SHIFL (Lactoylglutathione lyase OS=Shigella flexneri GN=gloA PE=3 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.186e-38
Identity = 75/124 (60.48%), Postives = 93/124 (75.00%), Query Frame = 3 
Query:  111 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 482
            RLLH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 86.2705 bits (212), Expect = 1.801e-16
Identity = 41/64 (64.06%), Postives = 48/64 (75.00%), Query Frame = 3 
Query:  504 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEY 695
            L   MLRVGDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV +Y
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY 66
[Close]
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_ECOLI (Lactoylglutathione lyase OS=Escherichia coli (strain K12) GN=gloA PE=1 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.186e-38
Identity = 75/124 (60.48%), Postives = 93/124 (75.00%), Query Frame = 3 
Query:  111 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 482
            RLLH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 86.2705 bits (212), Expect = 1.801e-16
Identity = 41/64 (64.06%), Postives = 48/64 (75.00%), Query Frame = 3 
Query:  504 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEY 695
            L   MLRVGDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV +Y
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY 66
[Close]
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_ECO57 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 GN=gloA PE=3 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.186e-38
Identity = 75/124 (60.48%), Postives = 93/124 (75.00%), Query Frame = 3 
Query:  111 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 482
            RLLH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GPVKGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 86.2705 bits (212), Expect = 1.801e-16
Identity = 41/64 (64.06%), Postives = 48/64 (75.00%), Query Frame = 3 
Query:  504 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEY 695
            L   MLRVGDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV +Y
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY 66
[Close]
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_SALTY (Lactoylglutathione lyase OS=Salmonella typhimurium GN=gloA PE=3 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 4.871e-38
Identity = 75/124 (60.48%), Postives = 92/124 (74.19%), Query Frame = 3 
Query:  111 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 482
            RLLH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH A++ ++  +  E IR  GGNVTRE GPVKGG+T IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 83.9593 bits (206), Expect = 8.938e-16
Identity = 40/64 (62.50%), Postives = 46/64 (71.88%), Query Frame = 3 
Query:  504 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEY 695
            L   MLRVGDL RSI FY   LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV  Y
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESY 66
[Close]
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_SALTI (Lactoylglutathione lyase OS=Salmonella typhi GN=gloA PE=3 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 4.871e-38
Identity = 75/124 (60.48%), Postives = 92/124 (74.19%), Query Frame = 3 
Query:  111 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 482
            RLLH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH A++ ++  +  E IR  GGNVTRE GPVKGG+T IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 83.9593 bits (206), Expect = 8.938e-16
Identity = 40/64 (62.50%), Postives = 46/64 (71.88%), Query Frame = 3 
Query:  504 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEY 695
            L   MLRVGDL RSI FY   LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV  Y
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESY 66
[Close]
BLAST of FC875689 vs. ExPASy Swiss-Prot
Match: LGUL_SYNY3 (Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803) GN=gloA PE=3 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 5.385e-37
Identity = 69/123 (56.10%), Postives = 91/123 (73.98%), Query Frame = 3 
Query:  114 LLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPVKGGTTHIAFVKDPDGYIFELIQ 482
            LLH + RVGDLD++++FY +  GM LLRK+D P  +++ AF+G+G E  + V+ELT+N+G   YD+G GFGH A+  ED+Y   + IR KGG V REPGP+K GTT IAFV+DPDGY  ELIQ
Sbjct:    3 LLHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKYDLGNGFGHIALGVEDIYSTCDKIRDKGGKVVREPGPMKHGTTVIAFVEDPDGYKIELIQ 125

HSP 2 Score: 68.1662 bits (165), Expect = 5.076e-11
Identity = 31/64 (48.44%), Postives = 44/64 (68.75%), Query Frame = 3 
Query:  504 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTEY 695
            L   M+RVGDL +S++FY   LGM LLR  D P  ++TLA +GY +E +  V+ELT+N+G  +Y
Sbjct:    3 LLHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKY 66
The following BLAST results are available for this feature:
BLAST of FC875689 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 28
Match NameE-valueIdentityDescription
LGUL_ORYSJ3.546e-9782.44Lactoylglutathione lyase OS=Oryza sativa subsp. ja... [more]
LGUL_BRAOG3.546e-9782.35Putative lactoylglutathione lyase OS=Brassica oler... [more]
LGUL_VIBPA2.759e-4162.90Probable lactoylglutathione lyase OS=Vibrio paraha... [more]
LGUL_VIBCH3.984e-4061.29Probable lactoylglutathione lyase OS=Vibrio choler... [more]
LGUL_SHIFL2.186e-3860.48Lactoylglutathione lyase OS=Shigella flexneri GN=g... [more]
LGUL_ECOLI2.186e-3860.48Lactoylglutathione lyase OS=Escherichia coli (stra... [more]
LGUL_ECO572.186e-3860.48Lactoylglutathione lyase OS=Escherichia coli O157:... [more]
LGUL_SALTY4.871e-3860.48Lactoylglutathione lyase OS=Salmonella typhimurium... [more]
LGUL_SALTI4.871e-3860.48Lactoylglutathione lyase OS=Salmonella typhi GN=gl... [more]
LGUL_SYNY35.385e-3756.10Probable lactoylglutathione lyase OS=Synechocystis... [more]

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Properties
Property NameValue
Genbank descriptionC31401G03EF CTVClemen1 Citrus clementina cDNA clone C31401G03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC875689 ID=FC875689; Name=FC875689; organism=Citrus clementina; type=EST; length=712bp
GACTTGCGACTTCACTCCGACTTTCTGAGTGATTCCATATCAAAATGGCT
GAGGCTTCTCCTGCTGCTGCTAATGCCGAGTTGCTGGAATGGCCAAAGAA
AGATAAGCGACGCCTCCTTCATGCTGTGTATCGTGTTGGTGATCTTGATC
GCACCATCAAATTTTACACCGAATGCTTTGGGATGAAGCTGTTGAGAAAG
AGAGACGTCCCGGAAGAGAAATACTCAAATGCCTTTCTTGGATTTGGCCC
CGAACAGTCTCACTTTGTTGTGGAGTTAACGTACAACTATGGTGTAACTT
CATATGATATTGGAACTGGTTTTGGCCATTTTGCAATTGCAACTGAAGAT
GTTTACAAACTGGTTGAAAACATTCGTGCCAAGGGTGGCAATGTCACTAG
AGAGCCTGGTCCAGTTAAAGGTGGAACAACTCACATTGCCTTTGTGAAGG
ATCCCGATGGTTATATTTTTGAACTCATCCAGAGAGGTCCAACTCCTGAA
CCACTCTGTCAAGTAATGCTTCGCGTTGGCGATCTTGGTCGCTCCATCAA
GTTCTATGAAAAGGCTTTGGGGATGAAACTGTTAAGGACAGTTGATAAAC
CTGAATACAAGTACACCCTGGCCATGCTTGGATATGCTGAGGAAGATCAG
ACTACTGTTCTGGAGTTAACTTATAATTATGGGGTGACTGAATATACCAG
GGNAATGCATAT
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