FC876499

Overview
NameFC876499
Unique NameFC876499
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length829
Libraries
Library NameType
EHRcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC876499 vs. ExPASy Swiss-Prot
Match: GRPE_SYNPW (Protein grpE OS=Synechococcus sp. (strain WH7803) GN=grpE PE=3 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 1.237e-25
Identity = 62/154 (40.26%), Postives = 90/154 (58.44%), Query Frame = 1
Query:  367 METLQSYKEALASNDDTKAAEIEALLKSFEDEKIDLERKVVNLSEELSAERARILRISADFDNFRKRTEKERLSLVTNAQGEVMERLLQVLDNFERAKTQIKVQTEGEEXINNSYQSIYKQLVEILXSLGVVPVETVGNPFDPLLHEAIMREDS 828
            +ET     E  +++D   AAE+     + E     LER+   L +E    R + +RI+ADFDNFRKR  +++  L        +  +L V+DNFERA+ Q+  Q E  + ++ SYQ +YKQLV++L  LGV P+  VG  FDP LHEA++RE S
Sbjct:   23 VETPNDPVETPSASDPGSAAEVSPQTGNNEARLEQLEREHTTLRDEHDVLRGQYMRIAADFDNFRKRQSRDQDDLKIQLTCSTLSEILPVVDNFERARQQLDPQGEEAQALHRSYQGLYKQLVDVLKQLGVAPMRVVGQEFDPTLHEAVLREPS 176          
BLAST of FC876499 vs. ExPASy Swiss-Prot
Match: GRPE_THEEB (Protein grpE OS=Thermosynechococcus elongatus (strain BP-1) GN=grpE PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.755e-25
Identity = 59/152 (38.82%), Postives = 93/152 (61.18%), Query Frame = 1
Query:  367 METLQSYKEALASNDDTKAAEIEALLKSFEDEKIDLERKVVNLSEELSAERARILRISADFDNFRKRTEKERLSLVTNAQGEVMERLLQVLDNFERAKTQIKVQTEGEEXINNSYQSIYKQLVEILXSLGVVPVETVGNPFDPLLHEAIMRE 822
            +E +++  EA    +D  +    A      ++   LE    +LS+ +    ++ +R++ADF+NFRKRT++E+  L    +  V+  LL V+D+FE A+T I+ +TE EE I+ SYQ +YKQLVE L  +GV  ++  G PFDP LHEA++RE
Sbjct:   48 VENVENPAEATPGEEDQASEATSANAADLLEQIAALEAAKASLSQVVEERNSQYIRLAADFENFRKRTQREKEELELQIKCSVIADLLPVVDSFELARTHIQTETEAEEKIHRSYQGVYKQLVECLKRIGVSAMQAKGKPFDPNLHEAVLRE 199          
BLAST of FC876499 vs. ExPASy Swiss-Prot
Match: GRPE_SYNY3 (Protein grpE OS=Synechocystis sp. (strain PCC 6803) GN=grpE PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.367e-24
Identity = 54/130 (41.54%), Postives = 83/130 (63.85%), Query Frame = 1
Query:  433 EALLKSFEDEKIDLERKVVNLSEELSAERARILRISADFDNFRKRTEKERLSLVTNAQGEVMERLLQVLDNFERAKTQIKVQTEGEEXINNSYQSIYKQLVEILXSLGVVPVETVGNPFDPLLHEAIMRE 822
            E ++   + +     +++   SE+L + + R + ++A+FDNFRKRT++E+       +G  +  LL V+DNFERA+TQIK  ++GE  I+ SYQ +YK LV+ L  LGV P+   G PFDP  HEA++RE
Sbjct:   69 EEIIAILQKDLASHRQELAEQSEQLDSIKKRYVALAAEFDNFRKRTQREKEEQAKLIKGRTITELLPVVDNFERARTQIKPNSDGENQIHKSYQGVYKNLVDSLKGLGVAPMRPEGKPFDPKYHEAMLRE 198          
BLAST of FC876499 vs. ExPASy Swiss-Prot
Match: GRPE_SYNE7 (Protein grpE OS=Synechococcus elongatus (strain PCC 7942) GN=grpE PE=3 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 1.367e-24
Identity = 62/146 (42.47%), Postives = 91/146 (62.33%), Query Frame = 1
Query:  391 EALASNDDTKAAEIEALLKSFEDEKIDLERKVVNLSEELSAERARILRISADFDNFRKRTEKERLSLVTNAQGEVMERLLQVLDNFERAKTQIKVQTEGEEXINNSYQSIYKQLVEILXSLGVVPVETVGNPFDPLLHEAIMREDS 828
            EA++  D T A+  EA   + +   +  +R    L  EL  + +  LR++ADF+NFR+RT KER  L   ++   +  LL V+DNF+RA+ QIK Q E  E I+ SYQ +YKQLV+ L  +GV P+   G PFDP LH+A++RE++
Sbjct:   21 EAVSEPDVTVASGQEAAELAAQLALVAADRD--RLKTELDEQNSAYLRLAADFENFRRRTLKEREELELQSKRTTITELLPVIDNFDRARAQIKPQGEEAEAIHKSYQGLYKQLVDCLKRIGVSPMRAEGQPFDPSLHDAVLREET 164          
BLAST of FC876499 vs. ExPASy Swiss-Prot
Match: GRPE_ANASP (Protein grpE OS=Anabaena sp. (strain PCC 7120) GN=grpE PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 3.978e-24
Identity = 54/118 (45.76%), Postives = 79/118 (66.95%), Query Frame = 1
Query:  469 DLERKVVNLSEELSAERARILRISADFDNFRKRTEKERLSLVTNAQGEVMERLLQVLDNFERAKTQIKVQTEGEEXINNSYQSIYKQLVEILXSLGVVPVETVGNPFDPLLHEAIMRE 822
            +L +++ +L  +L     + +RI+ADF+N+RKRT+KE+  L    +   +  LL ++DNFERA++ +K QTE E  I+ SYQ +YKQLV+ L  LGV P+   G  FDP LHEA+MRE
Sbjct:   79 ELTQQISSLKTQLDERSTQYMRIAADFENYRKRTQKEKEELDLQVKRNTILELLPIVDNFERARSHLKPQTESEMTIHKSYQGVYKQLVDSLKRLGVSPMRPEGQEFDPNLHEAVMRE 196          
BLAST of FC876499 vs. ExPASy Swiss-Prot
Match: GRPE_ANAVT (Protein grpE OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=grpE PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 5.196e-24
Identity = 54/118 (45.76%), Postives = 79/118 (66.95%), Query Frame = 1
Query:  469 DLERKVVNLSEELSAERARILRISADFDNFRKRTEKERLSLVTNAQGEVMERLLQVLDNFERAKTQIKVQTEGEEXINNSYQSIYKQLVEILXSLGVVPVETVGNPFDPLLHEAIMRE 822
            +L +++ +L  +L     + +RI+ADF+N+RKRT+KE+  L    +   +  LL ++DNFERA++ +K QTE E  I+ SYQ +YKQLV+ L  LGV P+   G  FDP LHEA+MRE
Sbjct:   79 ELTQQINSLKTQLDERSTQYMRIAADFENYRKRTQKEKEELDLQVKRNTILELLPIVDNFERARSHLKPQTESEMTIHKSYQGVYKQLVDSLKRLGVSPMRPEGQEFDPNLHEAVMRE 196          
BLAST of FC876499 vs. ExPASy Swiss-Prot
Match: GRPE_CYAP7 (Protein grpE OS=Cyanothece sp. (strain PCC 7424) GN=grpE PE=3 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 8.863e-24
Identity = 57/130 (43.85%), Postives = 85/130 (65.38%), Query Frame = 1
Query:  433 EALLKSFEDEKIDLERKVVNLSEELSAERARILRISADFDNFRKRTEKERLSLVTNAQGEVMERLLQVLDNFERAKTQIKVQTEGEEXINNSYQSIYKQLVEILXSLGVVPVETVGNPFDPLLHEAIMRE 822
            E+LL++ +  K  LE +    ++++ A + R + ++A+FDNFRKRT +E+  L   A+ + +  LL V+DNFERA+ QIK   EGE  I+ SYQ +YK LV+ L  LGV  +   G PFDP+ HEA++RE
Sbjct:  103 ESLLQTNQSLKDQLEEQ----NQQIDAAKRRYIGLAAEFDNFRKRTLREKEELEKQAKRKTLSELLTVVDNFERARLQIKPSNEGEGEIHKSYQGVYKNLVDSLKRLGVSAMRAEGEPFDPMYHEAMLRE 228          
BLAST of FC876499 vs. ExPASy Swiss-Prot
Match: GRPE_PROMS (Protein grpE OS=Prochlorococcus marinus (strain AS9601) GN=grpE PE=3 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.974e-23
Identity = 55/155 (35.48%), Postives = 94/155 (60.65%), Query Frame = 1
Query:  367 METLQSYKEALASNDDTKAAEIEAL-LKSFEDEKIDLERKVVNLSEELSAERARILRISADFDNFRKRTEKERLSLVTNAQGEVMERLLQVLDNFERAKTQIKVQTEGEEXINNSYQSIYKQLVEILXSLGVVPVETVGNPFDPLLHEAIMREDS 828
            +E + S +E    N++  + + E +  +  ++   + + ++  L +E    + + +RISADFDNFRKR  +++  L      + +  +L ++DNFERA+ Q+K ++E  + ++ SYQ +YKQLVE+L   GV P+  VG  FDP LHEA++RE S
Sbjct:   23 LENVSSAQELTTENNELSSQKTEEINTEELKNSISNNDARLEQLEKEHETLKNQYVRISADFDNFRKRQSRDQDDLKIQLVSKTLTAILPIVDNFERARQQLKPESEEAQSLHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPSLHEAVLREPS 177          
BLAST of FC876499 vs. ExPASy Swiss-Prot
Match: GRPE_PROM4 (Protein grpE OS=Prochlorococcus marinus (strain MIT 9211) GN=grpE PE=3 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 1.974e-23
Identity = 61/165 (36.97%), Postives = 92/165 (55.76%), Query Frame = 1
Query:  343 EEAPTS---FIMETLQSYKEALASNDDTKAAEIEALLKSFEDEKIDLERKVVNLSEELSAERARILRISADFDNFRKRTEKERLSLVTNAQGEVMERLLQVLDNFERAKTQIKVQTEGEEXINNSYQSIYKQLVEILXSLGVVPVETVGNPFDPLLHEAIMREDS 828
            E  PTS    +    +  +EA A  D T +       K    + +D E ++  L  E     ++ +RI+ADFDNFRKR  +++  L    Q   +  +L V+DNF+RA+ Q+  + E  + ++ SYQ +YKQLV++L  LGV P+  VG  FDP LHEA++RE S
Sbjct:   24 EAKPTSDEPSLSNVAEGSQEAGAETDVTSSDA-----KDSSSQALDNEARLEQLEREHETLNSQYMRIAADFDNFRKRQSRDQDDLRLQLQCNTLSSILPVVDNFDRARQQLNPEGEEAQALHKSYQGLYKQLVDVLKQLGVAPMRVVGQTFDPSLHEAVLREPS 183          
BLAST of FC876499 vs. ExPASy Swiss-Prot
Match: GRPE_TRIEI (Protein grpE OS=Trichodesmium erythraeum (strain IMS101) GN=grpE PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 2.579e-23
Identity = 63/143 (44.06%), Postives = 87/143 (60.84%), Query Frame = 1
Query:  397 LASNDDTKAAEIEALLK-SFEDEKIDLERKVVNLSEELSAERARILRISADFDNFRKRTEKERLSLVTNAQGEVMERLLQVLDNFERAKTQIKVQTEGEEXINNSYQSIYKQLVEILXSLGVVPVETVGNPFDPLLHEAIMRE 822
            + S + T   + + LLK ++E  +  LE     L E+ S  +    R+ ADFDNFRKRT+KE+  L T  +   +  LL V+DNFERA++ IK   +GE  I+ SYQS+YKQ+V+ L  LGV  +   G  FDP LHEA+MRE
Sbjct:   54 IQSTESTSKEKDQVLLKEAYELLQTQLETTKYQLEEKESQYK----RLGADFDNFRKRTQKEKEDLDTQVKCSTIMELLPVIDNFERARSHIKPANDGEMAIHKSYQSVYKQMVDSLKRLGVSVMRPEGQEFDPNLHEAVMRE 192          
The following BLAST results are available for this feature:
BLAST of FC876499 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 85
Match NameE-valueIdentityDescription
GRPE_SYNPW1.237e-2540.26Protein grpE OS=Synechococcus sp. (strain WH7803) ... [more]
GRPE_THEEB2.755e-2538.82Protein grpE OS=Thermosynechococcus elongatus (str... [more]
GRPE_SYNY31.367e-2441.54Protein grpE OS=Synechocystis sp. (strain PCC 6803... [more]
GRPE_SYNE71.367e-2442.47Protein grpE OS=Synechococcus elongatus (strain PC... [more]
GRPE_ANASP3.978e-2445.76Protein grpE OS=Anabaena sp. (strain PCC 7120) GN=... [more]
GRPE_ANAVT5.196e-2445.76Protein grpE OS=Anabaena variabilis (strain ATCC 2... [more]
GRPE_CYAP78.863e-2443.85Protein grpE OS=Cyanothece sp. (strain PCC 7424) G... [more]
GRPE_PROMS1.974e-2335.48Protein grpE OS=Prochlorococcus marinus (strain AS... [more]
GRPE_PROM41.974e-2336.97Protein grpE OS=Prochlorococcus marinus (strain MI... [more]
GRPE_TRIEI2.579e-2344.06Protein grpE OS=Trichodesmium erythraeum (strain I... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAN2CE04RM1 EHR Citrus clementina cDNA clone KN0AAN2CE04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC876499 ID=FC876499; Name=FC876499; organism=Citrus clementina; type=EST; length=829bp
GCACGAGGCATAAAAACGCCGCCGTTTCACGCATCTTCTCTTCCCGCCAC
ACGTGCAGCCCCCATTTCCTTAAAATCTCCGAAACCCATCTGTATTTCCT
TCAGACAACGTCTAATAAGCACCAGCAGACTTTATCATCGGTCAAATTCT
CTCCGGTTCGTAAGTGTTCAGTCTCTCCGGTTCGTAAAGTTCTCTCCTTT
AGCTTCAACTGGAGAAACTGAAACCCAAGAGGAAATTCAGGAACCCCAAA
TTGAGGAATCTTCCGATGGTGCTGTTGGCATTGAAGATGGCACTAGTGAC
GATGATAGCTCTGGAGCTACTAGTGATGACACCTCAGATGCTGAGGAAGC
ACCTACTTCATTCATTATGGAAACACTCCAATCATACAAAGAAGCTTTGG
CCAGTAATGACGATACCAAAGCTGCTGAAATAGAGGCTTTATTAAAATCC
TTTGAAGATGAGAAAATTGATCTTGAGAGGAAAGTTGTTAACTTGTCTGA
AGAATTGTCAGCTGAGAGGGCTCGTATTCTTAGGATCAGTGCTGACTTTG
ACAATTTTCGTAAGAGAACGGAGAAGGAACGCCTTTCACTGGTAACAAAT
GCTCAAGGAGAAGTCATGGAGAGATTGTTGCAAGTTTTGGATAATTTTGA
GAGAGCTAAAACTCAAATTAAGGTGCAGACCGAGGGAGAGGAGNAAATAA
ACAACAGCTATCAGAGCATATATAAGCAATTAGTGGAAATCCTANGATCA
CTTGGTGTTGTTCCTGTGGAGACGGTTGGGAATCCTTTTGATCCATTGCT
TCACGAAGCAATTATGCGCGAGGATTCCA
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