FC920847

Overview
NameFC920847
Unique NameFC920847
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length698
Libraries
Library NameType
RVDevelop1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig10260 contig Ccv1_Contig10260:1..696. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS2_CITSI (Chalcone synthase 2 OS=Citrus sinensis GN=CHS2 PE=2 SV=1)

HSP 1 Score: 365.155 bits (936), Expect = 1.071e-113
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 66.6254 bits (161), Expect = 1.071e-113
Identity = 32/33 (96.97%), Postives = 33/33 (100.00%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVLVVCSEITAVTFRGPADTHLDSLVGQA
Sbjct:  181 NKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 213
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS2_RUTGR (Chalcone synthase 2 OS=Ruta graveolens GN=CHS2 PE=2 SV=1)

HSP 1 Score: 342.428 bits (877), Expect = 1.558e-106
Identity = 167/178 (93.82%), Postives = 172/178 (96.63%), Query Frame = 1 
Query:   67 TVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            TV+EIR AQRADGPA VLAIGTATPA+ V QADYPDYYFRITKSEHMTELKEKFKRMCDKSMI+KRYMYLTE+ILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAA KAIKEWGQPKSKITHLIFCTTSGVDMPG DYQLTKL+GLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    5 TVEEIRKAQRADGPAAVLAIGTATPANYVTQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIRKRYMYLTEDILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAAVKAIKEWGQPKSKITHLIFCTTSGVDMPGCDYQLTKLLGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 182

HSP 2 Score: 65.4698 bits (158), Expect = 1.558e-106
Identity = 31/33 (93.94%), Postives = 33/33 (100.00%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            ++GARVLVVCSEITAVTFRGPADTHLDSLVGQA
Sbjct:  183 NRGARVLVVCSEITAVTFRGPADTHLDSLVGQA 215
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS3_RUTGR (Chalcone synthase 3 OS=Ruta graveolens GN=CHS3 PE=2 SV=1)

HSP 1 Score: 342.428 bits (877), Expect = 4.515e-106
Identity = 167/178 (93.82%), Postives = 173/178 (97.19%), Query Frame = 1 
Query:   67 TVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            TV+EIR AQRADGPATVLAIGTATPA+ V QADYPDYYFRITKSEHMT+LKEKFKRMCDKSMI+KRYM+LTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAA KAIKEWGQPKSKITHLIFCTTSGVDMPG DYQLTKL+GLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    5 TVEEIRKAQRADGPATVLAIGTATPANYVTQADYPDYYFRITKSEHMTDLKEKFKRMCDKSMIRKRYMHLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAAAKAIKEWGQPKSKITHLIFCTTSGVDMPGCDYQLTKLLGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 182

HSP 2 Score: 63.929 bits (154), Expect = 4.515e-106
Identity = 30/33 (90.91%), Postives = 32/33 (96.97%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            ++GARVLVVCSEITAVTFRGP DTHLDSLVGQA
Sbjct:  183 NRGARVLVVCSEITAVTFRGPVDTHLDSLVGQA 215
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS1_RUTGR (Chalcone synthase 1 OS=Ruta graveolens GN=CHS1 PE=2 SV=1)

HSP 1 Score: 338.191 bits (866), Expect = 2.906e-105
Identity = 165/178 (92.70%), Postives = 171/178 (96.07%), Query Frame = 1 
Query:   67 TVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            TV+ IR AQRADGPA VLAIGTATPA+ V QADYPDYYFRITKSEHMTELKEKFKRMCDKSMI+KRYM+LTE+ILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAA KAIKEWGQPKSKITHLIFCTTSGVDMPG DYQLTKL+GLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    5 TVEAIRKAQRADGPAAVLAIGTATPANYVTQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIRKRYMHLTEDILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAAVKAIKEWGQPKSKITHLIFCTTSGVDMPGCDYQLTKLLGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 182

HSP 2 Score: 65.4698 bits (158), Expect = 2.906e-105
Identity = 31/33 (93.94%), Postives = 33/33 (100.00%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            ++GARVLVVCSEITAVTFRGPADTHLDSLVGQA
Sbjct:  183 NRGARVLVVCSEITAVTFRGPADTHLDSLVGQA 215
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHSY_BETVE (Chalcone synthase OS=Betula verrucosa GN=CHS PE=2 SV=2)

HSP 1 Score: 337.806 bits (865), Expect = 4.947e-105
Identity = 164/180 (91.11%), Postives = 172/180 (95.56%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            MA+V+EIR AQRA GPATVLAIGTATP++ + QADYPDYYFRITKS+HMTELKEKFKRMCDKSMIKKRYMYL EEIL ENPNMCAYMAPSLDARQ IVVVEVPKLGKEAATKAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GLRPSVKR MMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MASVEEIRKAQRAHGPATVLAIGTATPSNCITQADYPDYYFRITKSDHMTELKEKFKRMCDKSMIKKRYMYLNEEILNENPNMCAYMAPSLDARQTIVVVEVPKLGKEAATKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLRPSVKRLMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 65.0846 bits (157), Expect = 4.947e-105
Identity = 31/33 (93.94%), Postives = 32/33 (96.97%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVLVVCSEITAVTFRGP DTHLDSLVGQA
Sbjct:  181 NKGARVLVVCSEITAVTFRGPTDTHLDSLVGQA 213
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS3_SOYBN (Chalcone synthase 3 OS=Glycine max GN=CHS3 PE=3 SV=1)

HSP 1 Score: 335.88 bits (860), Expect = 1.870e-104
Identity = 162/180 (90.00%), Postives = 174/180 (96.67%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            M +V+EIRNAQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MVSVEEIRNAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 65.0846 bits (157), Expect = 1.870e-104
Identity = 31/33 (93.94%), Postives = 32/33 (96.97%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVLVVCSEITAVTFRGP DTHLDSLVGQA
Sbjct:  181 NKGARVLVVCSEITAVTFRGPTDTHLDSLVGQA 213
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS1_SOYBN (Chalcone synthase 1 OS=Glycine max GN=CHS1 PE=3 SV=1)

HSP 1 Score: 333.569 bits (854), Expect = 9.225e-104
Identity = 161/180 (89.44%), Postives = 173/180 (96.11%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            M +V+EIR AQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MVSVEEIRKAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 65.0846 bits (157), Expect = 9.225e-104
Identity = 31/33 (93.94%), Postives = 32/33 (96.97%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVLVVCSEITAVTFRGP DTHLDSLVGQA
Sbjct:  181 NKGARVLVVCSEITAVTFRGPTDTHLDSLVGQA 213
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS5_SOYBN (Chalcone synthase 5 OS=Glycine max GN=CHS5 PE=3 SV=1)

HSP 1 Score: 332.413 bits (851), Expect = 2.049e-103
Identity = 160/180 (88.89%), Postives = 173/180 (96.11%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            M +V+EIR AQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VV+EVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MVSVEEIRQAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVMEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 65.0846 bits (157), Expect = 2.049e-103
Identity = 31/33 (93.94%), Postives = 32/33 (96.97%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVLVVCSEITAVTFRGP DTHLDSLVGQA
Sbjct:  181 NKGARVLVVCSEITAVTFRGPTDTHLDSLVGQA 213
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS2_SOYBN (Chalcone synthase 2 OS=Glycine max GN=CHS2 PE=3 SV=1)

HSP 1 Score: 332.413 bits (851), Expect = 2.049e-103
Identity = 160/180 (88.89%), Postives = 173/180 (96.11%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            M +V+EIR AQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VV+EVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MVSVEEIRKAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVMEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 65.0846 bits (157), Expect = 2.049e-103
Identity = 31/33 (93.94%), Postives = 32/33 (96.97%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVLVVCSEITAVTFRGP DTHLDSLVGQA
Sbjct:  181 NKGARVLVVCSEITAVTFRGPTDTHLDSLVGQA 213
[Close]
BLAST of FC920847 vs. ExPASy Swiss-Prot
Match: CHS2_CAMSI (Chalcone synthase 2 OS=Camellia sinensis GN=CHS2 PE=2 SV=1)

HSP 1 Score: 333.183 bits (853), Expect = 3.487e-103
Identity = 160/180 (88.89%), Postives = 172/180 (95.56%), Query Frame = 1 
Query:   61 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAEN 600
            M TV+E+R AQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEH TELKEKF+RMCDKSMIKKRYMYLTEEILKENPN+CAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GLRPSVKR MMYQQGCFAGGTVLRLAKDLAEN
Sbjct:    1 MVTVEEVRRAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHKTELKEKFQRMCDKSMIKKRYMYLTEEILKENPNVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLRPSVKRLMMYQQGCFAGGTVLRLAKDLAEN 180

HSP 2 Score: 63.5438 bits (153), Expect = 3.487e-103
Identity = 30/33 (90.91%), Postives = 32/33 (96.97%), Query Frame = 3 
Query:  600 HKGARVLVVCSEITAVTFRGPADTHLDSLVGQA 698
            +KGARVLVVCSEITAVTFRGP+D HLDSLVGQA
Sbjct:  181 NKGARVLVVCSEITAVTFRGPSDAHLDSLVGQA 213
The following BLAST results are available for this feature:
BLAST of FC920847 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 145
Match NameE-valueIdentityDescription
CHS2_CITSI1.071e-113100.00Chalcone synthase 2 OS=Citrus sinensis GN=CHS2 PE=... [more]
CHS2_RUTGR1.558e-10693.82Chalcone synthase 2 OS=Ruta graveolens GN=CHS2 PE=... [more]
CHS3_RUTGR4.515e-10693.82Chalcone synthase 3 OS=Ruta graveolens GN=CHS3 PE=... [more]
CHS1_RUTGR2.906e-10592.70Chalcone synthase 1 OS=Ruta graveolens GN=CHS1 PE=... [more]
CHSY_BETVE4.947e-10591.11Chalcone synthase OS=Betula verrucosa GN=CHS PE=2 ... [more]
CHS3_SOYBN1.870e-10490.00Chalcone synthase 3 OS=Glycine max GN=CHS3 PE=3 SV... [more]
CHS1_SOYBN9.225e-10489.44Chalcone synthase 1 OS=Glycine max GN=CHS1 PE=3 SV... [more]
CHS5_SOYBN2.049e-10388.89Chalcone synthase 5 OS=Glycine max GN=CHS5 PE=3 SV... [more]
CHS2_SOYBN2.049e-10388.89Chalcone synthase 2 OS=Glycine max GN=CHS2 PE=3 SV... [more]
CHS2_CAMSI3.487e-10388.89Chalcone synthase 2 OS=Camellia sinensis GN=CHS2 P... [more]

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Properties
Property NameValue
Genbank descriptionC32013G05EF RVDevelop1 Citrus clementina cDNA clone C32013G05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC920847 ID=FC920847; Name=FC920847; organism=Citrus clementina; type=EST; length=698bp
TCGCCGGGGGAACTCACCAAGCACGAGCCACCAACATATTCATTAAGGGT
TCAACTTGAAATGGCAACCGTTCAAGAGATCAGAAACGCTCAGCGTGCCG
ACGGCCCGGCCACCGTCCTCGCCATCGGTACGGCCACGCCTGCCCACAGT
GTCAACCAGGCTGATTATCCCGACTATTACTTCAGGATCACAAAGAGCGA
GCATATGACGGAGCTTAAAGAGAAGTTCAAGCGCATGTGTGACAAGTCGA
TGATTAAGAAGCGTTACATGTACTTAACGGAAGAGATTTTGAAGGAAAAC
CCCAACATGTGCGCTTACATGGCTCCATCACTCGACGCACGTCAAGACAT
TGTGGTGGTCGAAGTGCCGAAGCTCGGGAAAGAAGCTGCTACAAAGGCCA
TCAAAGAATGGGGCCAGCCCAAGTCTAAGATCACCCACCTCATCTTCTGC
ACCACCTCCGGCGTCGACATGCCCGGCGCCGACTACCAGCTCACCAAACT
GATCGGCCTGCGCCCCTCCGTCAAGCGCTTCATGATGTACCAACAAGGAT
GTTTCGCCGGTGGCACTGTTCTTCGCCTCGCTAAAGACTTGGCTGAGAAC
ACAAGGGCGCTCGCGTTCTTGTTGTCTGTTCGGAGATCACGGCGGTCACT
TTCCGCGGCCCTGCCGATACTCATCTTGATTCTCTTGTGGGNCAGGCT
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