FE659117

Overview
NameFE659117
Unique NameFE659117
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length497
Libraries
Library NameType
Hong Anliu sweet orange SSH librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FE659117 vs. ExPASy Swiss-Prot
Match: CYSP1_MAIZE (Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 7.362e-61
Identity = 106/165 (64.24%), Postives = 127/165 (76.97%), Query Frame = -2
Query:    2 ATGNLLSLSEQQLVDCDHSCDIKEKGTCDNGCSGGLMTNAYKYLIATGGLEEESSYPYIGKRGKCQFDSEKIAVRVANFTNIPVDENQIAAHLVHHGPLAIGLNAIFMQTYIGGVSCPLICGKKWLNHGVLLVGYGAKGFSILRLGNQPYWIIKNSWGKQWGDHG 496
            ATG L  LSEQQ VDCDH CD  E  +CD+GC+GGLMT A+ YL   GGLE E  YPY G  GKC+FD  KI   V NF+ + VDE QI+A+L+ HGPLAIG+NA +MQTYIGGVSCP ICG+  L+HGVLLVGYGA GF+ +RL ++PYWIIKNSWG+ WG++G
Sbjct:  177 ATGKLEVLSEQQFVDCDHECDSSEPDSCDSGCNGGLMTTAFSYLQKAGGLESEKDYPYTGSDGKCKFDKSKIVASVQNFSVVSVDEAQISANLIKHGPLAIGINAAYMQTYIGGVSCPYICGRH-LDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENG 340          
BLAST of FE659117 vs. ExPASy Swiss-Prot
Match: A494_ARATH (Probable cysteine proteinase A494 OS=Arabidopsis thaliana GN=At2g21430 PE=2 SV=2)

HSP 1 Score: 224.172 bits (570), Expect = 2.618e-58
Identity = 101/166 (60.84%), Postives = 131/166 (78.92%), Query Frame = -2
Query:    2 ATGNLLSLSEQQLVDCDHSCDIKEKGTCDNGCSGGLMTNAYKYLIATGGLEEESSYPYIG-KRGKCQFDSEKIAVRVANFTNIPVDENQIAAHLVHHGPLAIGLNAIFMQTYIGGVSCPLICGKKWLNHGVLLVGYGAKGFSILRLGNQPYWIIKNSWGKQWGDHG 496
            ATG L+SLSEQQLVDCDH CD +E+G+CD+GC+GGLM +A++Y + TGGL  E  YPY G   G C+ D  KI   V+NF+ + ++E+QIAA+L+ +GPLA+ +NA +MQTYIGGVSCP IC ++ LNHGVLLVGYG+ GFS  RL  +PYWIIKNSWG+ WG++G
Sbjct:  172 ATGKLVSLSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGSCKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRR-LNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENG 336          
BLAST of FE659117 vs. ExPASy Swiss-Prot
Match: RD19A_ARATH (Cysteine proteinase RD19a OS=Arabidopsis thaliana GN=RD19A PE=2 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 5.833e-58
Identity = 103/166 (62.05%), Postives = 130/166 (78.31%), Query Frame = -2
Query:    2 ATGNLLSLSEQQLVDCDHSCDIKEKGTCDNGCSGGLMTNAYKYLIATGGLEEESSYPYIGKRGK-CQFDSEKIAVRVANFTNIPVDENQIAAHLVHHGPLAIGLNAIFMQTYIGGVSCPLICGKKWLNHGVLLVGYGAKGFSILRLGNQPYWIIKNSWGKQWGDHG 496
            ATG L+SLSEQQLVDCDH CD +E  +CD+GC+GGLM +A++Y + TGGL +E  YPY GK GK C+ D  KI   V+NF+ I +DE QIAA+LV +GPLA+ +NA +MQTYIGGVSCP IC ++ LNHGVLLVGYGA G++  R   +PYWIIKNSWG+ WG++G
Sbjct:  175 ATGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPYTGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYICTRR-LNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENG 339          
BLAST of FE659117 vs. ExPASy Swiss-Prot
Match: CYSP_PEA (Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 2.895e-57
Identity = 94/165 (56.97%), Postives = 129/165 (78.18%), Query Frame = -2
Query:    2 ATGNLLSLSEQQLVDCDHSCDIKEKGTCDNGCSGGLMTNAYKYLIATGGLEEESSYPYIGKRGKCQFDSEKIAVRVANFTNIPVDENQIAAHLVHHGPLAIGLNAIFMQTYIGGVSCPLICGKKWLNHGVLLVGYGAKGFSILRLGNQPYWIIKNSWGKQWGDHG 496
            ATG L+SLSEQQLVDCDH CD ++ G+CD+GC+GGLM NA++YL+ +GG+ +E  Y Y G+ G C+FD  K+   V+NF+ + +DE+QIAA+LV +GPLA+ +NA +MQTY+ GVSCP +C K  L+HGVLLVG+G   ++ +RL  +PYWIIKNSWG+ WG+ G
Sbjct:  172 ATGKLVSLSEQQLVDCDHVCDPEQAGSCDSGCNGGLMNNAFEYLLESGGVVQEKDYAYTGRDGSCKFDKSKVVASVSNFSVVTLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQG 336          
BLAST of FE659117 vs. ExPASy Swiss-Prot
Match: CYSP1_DICDI (Cysteine proteinase 1 OS=Dictyostelium discoideum GN=cprA PE=1 SV=2)

HSP 1 Score: 167.933 bits (424), Expect = 2.227e-41
Identity = 83/163 (50.92%), Postives = 110/163 (67.48%), Query Frame = -2
Query:    2 LLSLSEQQLVDCDHSC-DIKEKGTCDNGCSGGLMTNAYKYLIATGGLEEESSYPYIGKRG-KCQFDSEKIAVRVANFTNIPVDENQIAAHLVHHGPLAIGLNAIFMQTYIGGVSCPLICGKKWLNHGVLLVGYGAKGFSILRLGNQPYWIIKNSWGKQWGDHG 484
            L+SLSEQ LVDCDH C + + +  CD GC+GGL  NAY Y+I  GG++ ESSYPY  + G +C F+S  I  +++NFT IP +E  +A ++V  GPLAI  +A+  Q YIGGV   + C    L+HG+L+VGY AK  +I R  N PYWI+KNSWG  WG+ G
Sbjct:  162 LVSLSEQNLVDCDHECMEYEGEQACDEGCNGGLQPNAYNYIIKNGGIQTESSYPYTAETGTQCNFNSANIGAKISNFTMIPKNETVMAGYIVSTGPLAIAADAVEWQFYIGGV-FDIPCNPNSLDHGILIVGYSAKN-TIFR-KNMPYWIVKNSWGADWGEQG 321          
BLAST of FE659117 vs. ExPASy Swiss-Prot
Match: CATL_SCHMA (Cathepsin L OS=Schistosoma mansoni GN=CL1 PE=2 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 4.805e-36
Identity = 75/166 (45.18%), Postives = 105/166 (63.25%), Query Frame = -2
Query:    2 TGNLLSLSEQQLVDCDHSCDIKEKGTCDNGCSGGLMTNAYKYLIATGGLEEESSYPYIGKRGKCQFDSEKIAVRVANFTNIPVDENQIAAHLVHHGPLAIGLNAIFMQTYIGGVSCP--LICGKKWLNHGVLLVGYGAKGFSILRLGNQPYWIIKNSWGKQWGDHG 493
            TG LLSLSEQQLVDCD           D+GC+GGL +NAY+ +I  GGL  E +YPY  K  KC   ++ +AV + +  N+  DE ++AA L H+  +++G+NA+ +Q Y  G+S P  + C K  L+H VLLVGYG          N+P+WI+KNSWG +WG++G
Sbjct:  146 TGKLLSLSEQQLVDCDG---------LDDGCNGGLPSNAYESIIKMGGLMLEDNYPYDAKNEKCHLKTDGVAVYINSSVNLTQDETELAAWLYHNSTISVGMNALLLQFYQHGISHPWWIFCSKYLLDHAVLLVGYGVSE------KNEPFWIVKNSWGVEWGENG 296          
BLAST of FE659117 vs. ExPASy Swiss-Prot
Match: CPR1_DROME (Putative cysteine proteinase CG12163 OS=Drosophila melanogaster GN=CG12163 PE=2 SV=2)

HSP 1 Score: 145.591 bits (366), Expect = 1.183e-34
Identity = 77/167 (46.11%), Postives = 103/167 (61.68%), Query Frame = -2
Query:    2 TGNLLSLSEQQLVDCDHSCDIKEKGTCDNGCSGGLMTNAYKYLIATGGLEEESSYPYIGKRGKCQFDSEKIAVRVANFTNIPV-DENQIAAHLVHHGPLAIGLNAIFMQTYIGGVSCP--LICGKKWLNHGVLLVGYGAKGFSILRLGNQPYWIIKNSWGKQWGDHG 493
            TG L   SEQ+L+DCD         T D+ C+GGLM NAYK +   GGLE E+ YPY  K+ +C F+     V+VA F ++P  +E  +   L+ +GP++IG+NA  MQ Y GGVS P   +C KK L+HGVL+VGYG   +        PYWI+KNSWG +WG+ G
Sbjct:  435 TGELKEFSEQELLDCD---------TTDSACNGGLMDNAYKAIKDIGGLEYEAEYPYKAKKNQCHFNRTLSHVQVAGFVDLPKGNETAMQEWLLANGPISIGINANAMQFYRGGVSHPWKALCSKKNLDHGVLVVGYGVSDYPNFH-KTLPYWIVKNSWGPRWGEQG 591          
BLAST of FE659117 vs. ExPASy Swiss-Prot
Match: CYSP_TRYBB (Cysteine proteinase OS=Trypanosoma brucei brucei PE=1 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 5.146e-30
Identity = 70/166 (42.17%), Postives = 98/166 (59.04%), Query Frame = -2
Query:    2 LLSLSEQQLVDCDHSCDIKEKGTCDNGCSGGLMTNAYKYLIATGG--LEEESSYPYI---GKRGKCQFDSEKIAVRVANFTNIPVDENQIAAHLVHHGPLAIGLNAIFMQTYIGGVSCPLICGKKWLNHGVLLVGYGAKGFSILRLGNQPYWIIKNSWGKQWGDHG 484
            L+SLSEQ LV CD         T D+GC+GGLM NA+ +++ + G  +  E+SYPY+   G++ +CQ +  +I   + +  ++P DE+ IAA+L  +GPLAI ++A     Y GG+     C  K L+HGVLLVGY           N PYWIIKNSW   WG+ G
Sbjct:  170 LVSLSEQMLVSCD---------TIDSGCNGGLMDNAFNWIVNSNGGNVFTEASYPYVSGNGEQPQCQMNGHEIGAAITDHVDLPQDEDAIAAYLAENGPLAIAVDAESFMDYNGGILTS--CTSKQLDHGVLLVGYNDN-------SNPPYWIIKNSWSNMWGEDG 317          
BLAST of FE659117 vs. ExPASy Swiss-Prot
Match: CATL1_RAT (Cathepsin L1 OS=Rattus norvegicus GN=Ctsl1 PE=1 SV=2)

HSP 1 Score: 128.642 bits (322), Expect = 1.497e-29
Identity = 67/166 (40.36%), Postives = 95/166 (57.23%), Query Frame = -2
Query:    2 TGNLLSLSEQQLVDCDHSCDIKEKGTCDNGCSGGLMTNAYKYLIATGGLEEESSYPYIGKRGKCQFDSEKIAVRVANFTNIPVDENQIAAHLVHHGPLAIGLNAIF--MQTYIGGVSCPLICGKKWLNHGVLLVGYGAKGFSILRLGNQPYWIIKNSWGKQWGDHG 493
            TG L+SLSEQ LVDC H     ++G  + GC+GGLM  A++Y+   GGL+ E SYPY  K G C++ +E        F +IP  E  +   +   GP+++ ++A    +Q Y  G+     C  K L+HGVL+VGYG +G          YW++KNSWGK+WG  G
Sbjct:  155 TGKLISLSEQNLVDCSH-----DQG--NQGCNGGLMDFAFQYIKENGGLDSEESYPYEAKDGSCKYRAEYAVANDTGFVDIPQQEKALMKAVATVGPISVAMDASHPSLQFYSSGIYYEPNCSSKDLDHGVLVVGYGYEGTD---SNKDKYWLVKNSWGKEWGMDG 310          
BLAST of FE659117 vs. ExPASy Swiss-Prot
Match: CATL1_MOUSE (Cathepsin L1 OS=Mus musculus GN=Ctsl1 PE=1 SV=2)

HSP 1 Score: 127.487 bits (319), Expect = 3.335e-29
Identity = 66/166 (39.76%), Postives = 92/166 (55.42%), Query Frame = -2
Query:    2 TGNLLSLSEQQLVDCDHSCDIKEKGTCDNGCSGGLMTNAYKYLIATGGLEEESSYPYIGKRGKCQFDSEKIAVRVANFTNIPVDENQIAAHLVHHGPLAIGLNAIF--MQTYIGGVSCPLICGKKWLNHGVLLVGYGAKGFSILRLGNQPYWIIKNSWGKQWGDHG 493
            TG L+SLSEQ LVDC H+         + GC+GGLM  A++Y+   GGL+ E SYPY  K G C++ +E        F +IP  E  +   +   GP+++ ++A    +Q Y  G+     C  K L+HGVLLVGYG +G          YW++KNSWG +WG  G
Sbjct:  155 TGKLISLSEQNLVDCSHAQG-------NQGCNGGLMDFAFQYIKENGGLDSEESYPYEAKDGSCKYRAEFAVANDTGFVDIPQQEKALMKAVATVGPISVAMDASHPSLQFYSSGIYYEPNCSSKNLDHGVLLVGYGYEGTD---SNKNKYWLVKNSWGSEWGMEG 310          
The following BLAST results are available for this feature:
BLAST of FE659117 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 153
Match NameE-valueIdentityDescription
CYSP1_MAIZE7.362e-6164.24Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=... [more]
A494_ARATH2.618e-5860.84Probable cysteine proteinase A494 OS=Arabidopsis t... [more]
RD19A_ARATH5.833e-5862.05Cysteine proteinase RD19a OS=Arabidopsis thaliana ... [more]
CYSP_PEA2.895e-5756.97Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1[more]
CYSP1_DICDI2.227e-4150.92Cysteine proteinase 1 OS=Dictyostelium discoideum ... [more]
CATL_SCHMA4.805e-3645.18Cathepsin L OS=Schistosoma mansoni GN=CL1 PE=2 SV=... [more]
CPR1_DROME1.183e-3446.11Putative cysteine proteinase CG12163 OS=Drosophila... [more]
CYSP_TRYBB5.146e-3042.17Cysteine proteinase OS=Trypanosoma brucei brucei P... [more]
CATL1_RAT1.497e-2940.36Cathepsin L1 OS=Rattus norvegicus GN=Ctsl1 PE=1 SV... [more]
CATL1_MOUSE3.335e-2939.76Cathepsin L1 OS=Mus musculus GN=Ctsl1 PE=1 SV=2[more]

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Properties
Property NameValue
Genbank descriptionC56 Hong Anliu sweet orange SSH library Citrus sinensis cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FE659117 ID=FE659117; Name=FE659117; organism=Citrus sinensis; type=EST; length=497bp
ACCCATGGTCTCCCCATTGCTTGCCCCACGAATTCTTGATGATCCAATAC
GGCTGGTTTCCAAGCCTAAGAATTGAGAAGCCCTTGGCACCATACCCGAC
GAGCAGAACGCCGTGGTTGAGCCATTTCTTGCCGCAGATTAGCGGGCATG
AGACTCCCCCAATGTATGTTTGCATGAATATAGCGTTCAACCCAATAGCC
AGAGGACCGTGGTGGACTAAATGTGCAGCAATTTGGTTCTCATCGACAGG
AATATTGGTAAAATTTGCAACTCGAACGGCTATTTTCTCTGAATCGAATT
GGCATTTGCCACGCTTCCCAATATAAGGATATGAACTCTCCTCTTCTAGC
CCTCCAGTCGCGATCAAATATTTGTATGCATTTGTCATGAGGCCTCCAGA
ACAACCATTGTCACAAGTACCCTTCTCCTTTATATCACACGAGTGATCAC
AATCTACAAGCTGCTGCTCGCTAAGACTGAGAAGATTCCCAGTCGCA
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