EY711075

Overview
NameEY711075
Unique NameEY711075
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length899
Libraries
Library NameType
Sweet orange fruit, development stadium (3 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY711075 vs. ExPASy Swiss-Prot
Match: ODBA_DICDI (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Dictyostelium discoideum GN=bkdA PE=3 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.156e-35
Identity = 68/125 (54.40%), Postives = 89/125 (71.20%), Query Frame = 2
Query:  452 VPCFRVLDDNGELIKGSDFQQVSKEVAVKMYSHMVTLQVMDSVLYEAQRQGRFSFYLTTIGEEAINIGSAAALSADDFILPQYREPGVLLWRGYTLQQFANQVFANKADDGKGRQMPIHYGSKKL 826
            +PC+ ++D  G + K       SKE  +KMY+ M+TL VMDS+LY+ QRQGR SFY+T+ GEEAI+IGSAAAL   D I  QYRE GV +WRG+T+    NQ   N+ D GKGRQMP+H+GS+K+
Sbjct:   64 IPCYTIMDQEGVVSKPDQDPNFSKEEVIKMYTTMLTLNVMDSILYDVQRQGRISFYMTSFGEEAIHIGSAAALEMSDTIFAQYRETGVFMWRGFTINDIINQCCTNEHDLGKGRQMPMHFGSRKI 188          

HSP 2 Score: 21.1718 bits (43), Expect = 3.156e-35
Identity = 8/12 (66.67%), Postives = 9/12 (75.00%), Query Frame = 1
Query:  835 TISSPIATHFPQ 870
            TISSP+ T  PQ
Sbjct:  192 TISSPLTTQLPQ 203          
BLAST of EY711075 vs. ExPASy Swiss-Prot
Match: ODBA_RAT (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (Fragment) OS=Rattus norvegicus GN=Bckdha PE=1 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 5.355e-35
Identity = 66/146 (45.21%), Postives = 93/146 (63.70%), Query Frame = 2
Query:  386 FPGGKVGYTSEMRFIPESSEKRVPCFRVLDDNGELIKGSDFQQVSKEVAVKMYSHMVTLQVMDSVLYEAQRQGRFSFYLTTIGEEAINIGSAAALSADDFILPQYREPGVLLWRGYTLQQFANQVFANKADDGKGRQMPIHYGSKK 823
            FPG    +  ++ FI  +    +P +RV+D  G++I  S+   + +E  +K+Y  M  L  MD +LYE+QRQGR SFY+T  GEE  ++GSAAAL   D +  QYRE GVL++R Y L+ F  Q + N +D GKGRQMP+HYG K+
Sbjct:   50 FPGASAEFVDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEEVLKLYRSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYRDYPLELFMAQCYGNVSDPGKGRQMPVHYGCKE 195          

HSP 2 Score: 25.7942 bits (55), Expect = 5.355e-35
Identity = 9/15 (60.00%), Postives = 13/15 (86.67%), Query Frame = 1
Query:  826 YYITISSPIATHFPQ 870
            +++TISSP+AT  PQ
Sbjct:  197 HFVTISSPLATQIPQ 211          
BLAST of EY711075 vs. ExPASy Swiss-Prot
Match: ODBA_PANTR (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 6.973e-35
Identity = 67/146 (45.89%), Postives = 93/146 (63.70%), Query Frame = 2
Query:  386 FPGGKVGYTSEMRFIPESSEKRVPCFRVLDDNGELIKGSDFQQVSKEVAVKMYSHMVTLQVMDSVLYEAQRQGRFSFYLTTIGEEAINIGSAAALSADDFILPQYREPGVLLWRGYTLQQFANQVFANKADDGKGRQMPIHYGSKK 823
            FPG    +  ++ FI  +    +P +RV+D  G++I  S+   + KE  +K+Y  M  L  MD +LYE+QRQGR SFY+T  GEE  ++GSAAAL   D +  QYRE GVL++R Y L+ F  Q + N +D GKGRQMP+HYG K+
Sbjct:   54 FPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKE 199          

HSP 2 Score: 25.7942 bits (55), Expect = 6.973e-35
Identity = 9/15 (60.00%), Postives = 13/15 (86.67%), Query Frame = 1
Query:  826 YYITISSPIATHFPQ 870
            +++TISSP+AT  PQ
Sbjct:  201 HFVTISSPLATQIPQ 215          
BLAST of EY711075 vs. ExPASy Swiss-Prot
Match: ODBA_MACFA (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Macaca fascicularis GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 6.973e-35
Identity = 67/146 (45.89%), Postives = 93/146 (63.70%), Query Frame = 2
Query:  386 FPGGKVGYTSEMRFIPESSEKRVPCFRVLDDNGELIKGSDFQQVSKEVAVKMYSHMVTLQVMDSVLYEAQRQGRFSFYLTTIGEEAINIGSAAALSADDFILPQYREPGVLLWRGYTLQQFANQVFANKADDGKGRQMPIHYGSKK 823
            FPG    +  ++ FI  +    +P +RV+D  G++I  S+   + KE  +K+Y  M  L  MD +LYE+QRQGR SFY+T  GEE  ++GSAAAL   D +  QYRE GVL++R Y L+ F  Q + N +D GKGRQMP+HYG K+
Sbjct:   54 FPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKE 199          

HSP 2 Score: 25.7942 bits (55), Expect = 6.973e-35
Identity = 9/15 (60.00%), Postives = 13/15 (86.67%), Query Frame = 1
Query:  826 YYITISSPIATHFPQ 870
            +++TISSP+AT  PQ
Sbjct:  201 HFVTISSPLATQIPQ 215          
BLAST of EY711075 vs. ExPASy Swiss-Prot
Match: ODBA_HUMAN (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Homo sapiens GN=BCKDHA PE=1 SV=2)

HSP 1 Score: 143.28 bits (360), Expect = 6.973e-35
Identity = 67/146 (45.89%), Postives = 93/146 (63.70%), Query Frame = 2
Query:  386 FPGGKVGYTSEMRFIPESSEKRVPCFRVLDDNGELIKGSDFQQVSKEVAVKMYSHMVTLQVMDSVLYEAQRQGRFSFYLTTIGEEAINIGSAAALSADDFILPQYREPGVLLWRGYTLQQFANQVFANKADDGKGRQMPIHYGSKK 823
            FPG    +  ++ FI  +    +P +RV+D  G++I  S+   + KE  +K+Y  M  L  MD +LYE+QRQGR SFY+T  GEE  ++GSAAAL   D +  QYRE GVL++R Y L+ F  Q + N +D GKGRQMP+HYG K+
Sbjct:   54 FPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKE 199          

HSP 2 Score: 25.7942 bits (55), Expect = 6.973e-35
Identity = 9/15 (60.00%), Postives = 13/15 (86.67%), Query Frame = 1
Query:  826 YYITISSPIATHFPQ 870
            +++TISSP+AT  PQ
Sbjct:  201 HFVTISSPLATQIPQ 215          
BLAST of EY711075 vs. ExPASy Swiss-Prot
Match: ODBA_MOUSE (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus GN=Bckdha PE=1 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 2.007e-34
Identity = 65/146 (44.52%), Postives = 92/146 (63.01%), Query Frame = 2
Query:  386 FPGGKVGYTSEMRFIPESSEKRVPCFRVLDDNGELIKGSDFQQVSKEVAVKMYSHMVTLQVMDSVLYEAQRQGRFSFYLTTIGEEAINIGSAAALSADDFILPQYREPGVLLWRGYTLQQFANQVFANKADDGKGRQMPIHYGSKK 823
            FPG    +  ++ FI  +    +P +RV+D  G++I  S+   + +E  +K Y  M  L  MD +LYE+QR+GR SFY+T  GEE  ++GSAAAL   D +  QYRE GVL++R Y L+ F +Q + N  D GKGRQMP+HYG K+
Sbjct:   51 FPGASAEFVDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEEVLKFYRSMTLLNTMDRILYESQREGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYRDYPLELFMSQCYGNVNDPGKGRQMPVHYGCKE 196          

HSP 2 Score: 25.7942 bits (55), Expect = 2.007e-34
Identity = 9/15 (60.00%), Postives = 13/15 (86.67%), Query Frame = 1
Query:  826 YYITISSPIATHFPQ 870
            +++TISSP+AT  PQ
Sbjct:  198 HFVTISSPLATQIPQ 212          
BLAST of EY711075 vs. ExPASy Swiss-Prot
Match: ODBA_BOVIN (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Bos taurus GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 5.774e-34
Identity = 65/146 (44.52%), Postives = 92/146 (63.01%), Query Frame = 2
Query:  386 FPGGKVGYTSEMRFIPESSEKRVPCFRVLDDNGELIKGSDFQQVSKEVAVKMYSHMVTLQVMDSVLYEAQRQGRFSFYLTTIGEEAINIGSAAALSADDFILPQYREPGVLLWRGYTLQQFANQVFANKADDGKGRQMPIHYGSKK 823
            FPG    +  ++ FI  +    +P +RV+D  G++I  S+   + +E  +K Y  M  L  MD +LYE+QRQGR SFY+T  GEE  ++GSAAAL   D +  QYRE GVL++R Y L+ F  Q + N +D GKGRQMP+HYG ++
Sbjct:   64 FPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEKVLKFYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDDTDLVFGQYREAGVLMYRDYPLELFMAQCYGNVSDLGKGRQMPVHYGCRE 209          

HSP 2 Score: 25.7942 bits (55), Expect = 5.774e-34
Identity = 9/15 (60.00%), Postives = 13/15 (86.67%), Query Frame = 1
Query:  826 YYITISSPIATHFPQ 870
            +++TISSP+AT  PQ
Sbjct:  211 HFVTISSPLATQIPQ 225          
BLAST of EY711075 vs. ExPASy Swiss-Prot
Match: ODBA_PSEAE (2-oxoisovalerate dehydrogenase subunit alpha OS=Pseudomonas aeruginosa GN=bkdA1 PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.188e-16
Identity = 50/130 (38.46%), Postives = 73/130 (56.15%), Query Frame = 2
Query:  434 ESSEKRVPCFRVLDDNGELIKGSDFQQVSKEVAVKMYSHMVTLQVMDSVLYEAQRQGRFSFYLTTIGEEAINIGSAAALSADDFILPQYREPGVLLWRGYTLQQFANQVFANKADDGKGRQMPIHYGSKK 823
            E+S+      RVLDD+G  + G    Q+S E  ++    M+  ++ D+ +  AQRQ + SFY+  +GEEAI      AL   D   P YR+ G+L+ R Y L     Q+ +N+AD  KGRQ+PI Y SK+
Sbjct:   46 ETSDLAYSLVRVLDDDGHAV-GPWNPQLSNEQLLRGMRAMLKTRLFDARMLTAQRQKKLSFYMQCLGEEAIATAHTLALRDGDMCFPTYRQQGILITREYPLVDMICQLLSNEADPLKGRQLPIMYSSKE 174          
BLAST of EY711075 vs. ExPASy Swiss-Prot
Match: ODBA_PSEPU (2-oxoisovalerate dehydrogenase subunit alpha OS=Pseudomonas putida GN=bkdA1 PE=1 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 2.477e-14
Identity = 42/120 (35.00%), Postives = 70/120 (58.33%), Query Frame = 2
Query:  464 RVLDDNGELIKGSDFQQVSKEVAVKMYSHMVTLQVMDSVLYEAQRQGRFSFYLTTIGEEAINIGSAAALSADDFILPQYREPGVLLWRGYTLQQFANQVFANKADDGKGRQMPIHYGSKK 823
            RVLD+ G+  +G   + +  ++  +    M+  ++ DS +  AQRQ + SFY+ ++GEEAI  G A AL+  D   P YR+  +L+ R  +L +   Q+ +N+ D  KGRQ+PI Y  ++
Sbjct:   56 RVLDEQGDA-QGPWAEDIDPQILRQGMRAMLKTRIFDSRMVVAQRQKKMSFYMQSLGEEAIGSGQALALNRTDMCFPTYRQQSILMARDVSLVEMICQLLSNERDPLKGRQLPIMYSVRE 174          
BLAST of EY711075 vs. ExPASy Swiss-Prot
Match: ODPA_BACST (Pyruvate dehydrogenase E1 component subunit alpha OS=Bacillus stearothermophilus GN=pdhA PE=1 SV=2)

HSP 1 Score: 73.1738 bits (178), Expect = 2.318e-12
Identity = 36/104 (34.62%), Postives = 56/104 (53.85%), Query Frame = 2
Query:  434 ESSEKRVPCFRVLDDNGELIKGSDFQQVSKEVAVKMYSHMVTLQVMDSVLYEAQRQGRFSFYLTTIGEEAINIGSAAALSADDFILPQYREPGVLLWRGYTLQQ 745
            E   ++ P F++L++ GE++      ++S E   ++   MV  +++D       RQGR  FY  T G+EA  I S  AL  +DFILP YR+   ++W G  L Q
Sbjct:   15 EKVAEQFPTFQILNEEGEVVNEEAMPELSDEQLKELMRRMVYTRILDQRSISLNRQGRLGFYAPTAGQEASQIASHFALEKEDFILPGYRDVPQIIWHGLPLYQ 118          
The following BLAST results are available for this feature:
BLAST of EY711075 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 23
Match NameE-valueIdentityDescription
ODBA_DICDI3.156e-3554.402-oxoisovalerate dehydrogenase subunit alpha, mito... [more]
ODBA_RAT5.355e-3545.212-oxoisovalerate dehydrogenase subunit alpha, mito... [more]
ODBA_PANTR6.973e-3545.892-oxoisovalerate dehydrogenase subunit alpha, mito... [more]
ODBA_MACFA6.973e-3545.892-oxoisovalerate dehydrogenase subunit alpha, mito... [more]
ODBA_HUMAN6.973e-3545.892-oxoisovalerate dehydrogenase subunit alpha, mito... [more]
ODBA_MOUSE2.007e-3444.522-oxoisovalerate dehydrogenase subunit alpha, mito... [more]
ODBA_BOVIN5.774e-3444.522-oxoisovalerate dehydrogenase subunit alpha, mito... [more]
ODBA_PSEAE1.188e-1638.462-oxoisovalerate dehydrogenase subunit alpha OS=Ps... [more]
ODBA_PSEPU2.477e-1435.002-oxoisovalerate dehydrogenase subunit alpha OS=Ps... [more]
ODPA_BACST2.318e-1234.62Pyruvate dehydrogenase E1 component subunit alpha ... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionCS00-C3-702-003-E04-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY711075 ID=EY711075; Name=EY711075; organism=Citrus sinensis; type=EST; length=899bp
CCACGCGTCCGCCCGTCAGTGCAAGGTACTGATTCCATCAAAAGCCTCGC
TGGCTACAAATCCCACCATCATTTTCTTCCTTCTTTCTTACCTTCGTATC
TTTGCCGTTGTTCAATATCGAAAAAATTAAATGGCGATTTGGTTAACAAA
ATCAAGAGCCATGGCCCAATGCTTACGGCAAAGAGTTGGTTTGCTGAGGA
TATTAAGTGGCCAGAGTTGTTCATCCCCATCAACAATAATATTCCCACTT
CAGCAATATAATTCATCATCACTTCCAAGCAAAACCCTTTTCTCGCCGGG
GCGTTCCGAGTCCACCGTTGCTTCCAATCAGCTGGATTCTTTGTCTTCCA
GTGACAGCGATGATGATCATCATCAGGTCTTGGATTTTCCCGGAGGCAAA
GTTGGATATACTTCTGAAATGAGGTTCATTCCTGAGTCTTCAGAGAAAAG
AGTGCCTTGTTTTCGAGTTCTTGATGACAATGGAGAGCTAATCAAGGGCA
GTGATTTTCAACAGGTGAGCAAGGAAGTTGCTGTAAAGATGTATAGCCAT
ATGGTCACACTGCAGGTAATGGACTCTGTATTGTACGAAGCACAAAGGCA
AGGGAGATTTTCTTTCTATCTTACAACAATTGGTGAAGAAGCAATCAACA
TAGGATCAGCAGCTGCACTCAGCGCCGATGATTTTATCTTGCCTCAGTAC
CGAGAGCCTGGAGTACTCTTATGGCGTGGCTACACATTGCAACAATTTGC
AAACCAGGTGTTTGCAAACAAGGCTGATGATGGGAAAGGCAGACAGATGC
CAATTCATTATGGGTCTAAAAAGCTTACTACATCACCATATCGTCACCTA
TCGCTACACACTTCCCTCAGCTGNNTGTGTGGCTATTTCTCTAAATGGA
back to top