EY684612

Overview
NameEY684612
Unique NameEY684612
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length871
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY684612 vs. ExPASy Swiss-Prot
Match: 3HIDH_ARATH (Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Arabidopsis thaliana GN=At4g20930 PE=2 SV=3)

HSP 1 Score: 315.464 bits (807), Expect = 2.550e-85
Identity = 150/191 (78.53%), Postives = 172/191 (90.05%), Query Frame = 2
Query:   11 DSWENPVMLDAPVSGGVLAAEAGTLTFMVGGSEDAYQAAKPLFLSMGKNTIYCGGAGNGAAAKICNNLTMAVSMLGVSEALTLGQSLGISASTLTKILNSSSARCWSSDSYNPVPGVMEGVPASRNYGGGFASKLMAKDLNLALASAKEVGVDCPLTSQAQDIYAKLCENGHDSKDFSCVFQHYYGGKDEV 583
            D+WE PVMLDAPVSGGVLAAEAGTLTFMVGG EDAY AA+P+  SMG+ +IYCGG+GNG+AAKICNNL MAVSMLG SEAL LGQSLGISASTLT++LN+SS RCWSSD+YNPVPGVM+GVP+SR+Y GGFASKLMAKDLNLA ASA+EVG   PL S+AQ+IY K+CE GH++KDFSCVF+H+Y GKDEV
Sbjct:  157 DNWEKPVMLDAPVSGGVLAAEAGTLTFMVGGPEDAYLAARPILQSMGRTSIYCGGSGNGSAAKICNNLAMAVSMLGTSEALALGQSLGISASTLTEVLNTSSGRCWSSDAYNPVPGVMKGVPSSRDYNGGFASKLMAKDLNLAAASAEEVGHKSPLISKAQEIYKKMCEEGHETKDFSCVFRHFYNGKDEV 347          
BLAST of EY684612 vs. ExPASy Swiss-Prot
Match: 3HIDH_BOVIN (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Bos taurus GN=HIBADH PE=2 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 5.733e-53
Identity = 103/176 (58.52%), Postives = 122/176 (69.32%), Query Frame = 2
Query:   29 VMLDAPVSGGVLAAEAGTLTFMVGGSEDAYQAAKPLFLSMGKNTIYCGGAGNGAAAKICNNLTMAVSMLGVSEALTLGQSLGISASTLTKILNSSSARCWSSDSYNPVPGVMEGVPASRNYGGGFASKLMAKDLNLALASAKEVGVDCPLTSQAQDIYAKLCENGHDSKDFSCVFQ 556
            V +DAPVSGGV AA +G LTFMVGG E+ + AA+ L   MG N +YCG  G G AAKICNNL +A+SM+G +EA+ LG  LG+    L KILN SS RCWSSD+YNPVPGVM+GVP++ NY GGF + LMAKDL LA  SA        L SQA  IY  +C  G+  KDFS VFQ
Sbjct:  153 VFMDAPVSGGVGAARSGNLTFMVGGVEEEFAAAQELLGCMGSNVVYCGAVGTGQAAKICNNLLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSANNYQGGFGTTLMAKDLGLAQDSATSTKSPILLGSQAHQIYRMMCAKGYSKKDFSSVFQ 328          
BLAST of EY684612 vs. ExPASy Swiss-Prot
Match: 3HIDH_PONAB (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Pongo abelii GN=HIBADH PE=2 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 2.179e-52
Identity = 102/176 (57.95%), Postives = 121/176 (68.75%), Query Frame = 2
Query:   29 VMLDAPVSGGVLAAEAGTLTFMVGGSEDAYQAAKPLFLSMGKNTIYCGGAGNGAAAKICNNLTMAVSMLGVSEALTLGQSLGISASTLTKILNSSSARCWSSDSYNPVPGVMEGVPASRNYGGGFASKLMAKDLNLALASAKEVGVDCPLTSQAQDIYAKLCENGHDSKDFSCVFQ 556
            V +DAPVSGGV AA +G LTFMVGG ED + AA+ L   MG N +YCG  G G AAKICNN+ +A+SM+G +EA+ LG  LG+    L KILN SS RCWSSD+YNPVPGVM+GVP++ NY GGF + LMAKDL LA  SA        L S A  IY  +C  G+  KDFS VFQ
Sbjct:  153 VFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAVGTGQAAKICNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSANNYQGGFGATLMAKDLGLAQDSATSTKSPILLGSLAHQIYRMMCAKGYSKKDFSSVFQ 328          
BLAST of EY684612 vs. ExPASy Swiss-Prot
Match: 3HIDH_HUMAN (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Homo sapiens GN=HIBADH PE=1 SV=2)

HSP 1 Score: 206.068 bits (523), Expect = 2.179e-52
Identity = 102/176 (57.95%), Postives = 121/176 (68.75%), Query Frame = 2
Query:   29 VMLDAPVSGGVLAAEAGTLTFMVGGSEDAYQAAKPLFLSMGKNTIYCGGAGNGAAAKICNNLTMAVSMLGVSEALTLGQSLGISASTLTKILNSSSARCWSSDSYNPVPGVMEGVPASRNYGGGFASKLMAKDLNLALASAKEVGVDCPLTSQAQDIYAKLCENGHDSKDFSCVFQ 556
            V +DAPVSGGV AA +G LTFMVGG ED + AA+ L   MG N +YCG  G G AAKICNN+ +A+SM+G +EA+ LG  LG+    L KILN SS RCWSSD+YNPVPGVM+GVP++ NY GGF + LMAKDL LA  SA        L S A  IY  +C  G+  KDFS VFQ
Sbjct:  153 VFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAVGTGQAAKICNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSANNYQGGFGTTLMAKDLGLAQDSATSTKSPILLGSLAHQIYRMMCAKGYSKKDFSSVFQ 328          
BLAST of EY684612 vs. ExPASy Swiss-Prot
Match: 3HIDH_MOUSE (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Mus musculus GN=Hibadh PE=2 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 3.716e-52
Identity = 101/177 (57.06%), Postives = 120/177 (67.80%), Query Frame = 2
Query:   29 VMLDAPVSGGVLAAEAGTLTFMVGGSEDAYQAAKPLFLSMGKNTIYCGGAGNGAAAKICNNLTMAVSMLGVSEALTLGQSLGISASTLTKILNSSSARCWSSDSYNPVPGVMEGVPASRNYGGGFASKLMAKDLNLALASAKEVGVDCPLTSQAQDIYAKLCENGHDSKDFSCVFQH 559
            V +DAPVSGGV AA +G LTFMVGG ED + AA+ L   MG N +YCG  G G +AKICNN+ +A+SM+G +EA+ LG   G+    L KILN SS RCWSSD+YNPVPGVM GVP+S NY GGF + LMAKDL LA  SA        L S A  IY  +C  G+  KDFS VFQ+
Sbjct:  152 VFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLECMGSNVVYCGAVGTGQSAKICNNMLLAISMIGTAEAMNLGIRSGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMHGVPSSNNYQGGFGTTLMAKDLGLAQDSATSTKTPILLGSLAHQIYRMMCSKGYSKKDFSSVFQY 328          
BLAST of EY684612 vs. ExPASy Swiss-Prot
Match: 3HIDH_RAT (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Rattus norvegicus GN=Hibadh PE=1 SV=3)

HSP 1 Score: 203.756 bits (517), Expect = 1.081e-51
Identity = 100/177 (56.50%), Postives = 122/177 (68.93%), Query Frame = 2
Query:   29 VMLDAPVSGGVLAAEAGTLTFMVGGSEDAYQAAKPLFLSMGKNTIYCGGAGNGAAAKICNNLTMAVSMLGVSEALTLGQSLGISASTLTKILNSSSARCWSSDSYNPVPGVMEGVPASRNYGGGFASKLMAKDLNLALASAKEVGVDCPLTSQAQDIYAKLCENGHDSKDFSCVFQH 559
            V +DAPVSGGV AA +G LTFMVGG E+ + AA+ L   MG N +YCG  G+G +AKICNN+ +A+SM+G +EA+ LG   G+    L KILN SS RCWSSD+YNPVPGVM+GVP+S NY GGF + LMAKDL LA  SA        L S A  IY  +C  G+  KDFS VFQ+
Sbjct:  152 VFMDAPVSGGVGAARSGNLTFMVGGVENEFAAAQELLGCMGSNVLYCGAVGSGQSAKICNNMLLAISMIGTAEAMNLGIRSGLDPKLLAKILNMSSGRCWSSDTYNPVPGVMDGVPSSNNYQGGFGTTLMAKDLGLAQDSATSTKTPILLGSVAHQIYRMMCSKGYSKKDFSSVFQY 328          
BLAST of EY684612 vs. ExPASy Swiss-Prot
Match: 3HIDH_DICDI (Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Dictyostelium discoideum GN=hibA PE=3 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 4.701e-47
Identity = 94/174 (54.02%), Postives = 112/174 (64.37%), Query Frame = 2
Query:   32 MLDAPVSGGVLAAEAGTLTFMVGGSEDAYQAAKPLFLSMGKNTIYCGGAGNGAAAKICNNLTMAVSMLGVSEALTLGQSLGISASTLTKILNSSSARCWSSDSYNPVPGVMEGVPASRNYGGGFASKLMAKDLNLALASAKEVGVDCPLTSQAQDIYAKLCENGHDSKDFSCVF 553
            MLD PVSGG   AEAGTLTFMVGGSE  +  AK     MGKN ++CG  G G  AK+CNNL + +SM+ VSEA+ LG   G+    L  I N+SSARCW+S+ YNP PGV+E  PASR Y GGF S LM KDL LA+ SAK +G    L + A  +Y  L   G   KDFS V+
Sbjct:  137 MLDCPVSGGTGGAEAGTLTFMVGGSEQDFNTAKTYLECMGKNIVHCGDVGTGQVAKVCNNLVLGISMIAVSEAMNLGVKQGMDPKKLAGIFNTSSARCWTSELYNPCPGVIETSPASRGYTGGFGSALMTKDLGLAVDSAKSIGEPLLLGNSAHQLYTLLVAKGDGQKDFSVVY 310          
BLAST of EY684612 vs. ExPASy Swiss-Prot
Match: 3HIDH_DROME (Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Drosophila melanogaster GN=CG15093 PE=1 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 4.400e-45
Identity = 89/173 (51.45%), Postives = 114/173 (65.90%), Query Frame = 2
Query:   35 LDAPVSGGVLAAEAGTLTFMVGGSEDAYQAAKPLFLSMGKNTIYCGGAGNGAAAKICNNLTMAVSMLGVSEALTLGQSLGISASTLTKILNSSSARCWSSDSYNPVPGVMEGVPASRNYGGGFASKLMAKDLNLALASAKEVGVDCPLTSQAQDIYAKLCENGHDSKDFSCVF 553
            +DAPVSGGV  AE  TLTFMVGG+E  Y A K +   MGK   +CG  G G AAK+CNN+ +A+SM+GVSEA+ L    G+ A+   +I+NSS+ RCW+S+ YNPVPGV    PA+R+Y GGF+S L+ KDL LA   A       PL S A  +Y  LC+ G  +KDFS V+
Sbjct:  142 IDAPVSGGVPGAEQATLTFMVGGTEAEYNAVKAVLECMGKKITHCGVYGMGQAAKLCNNMMLAISMIGVSEAMNLAVRQGLDANVFAEIINSSTGRCWASEIYNPVPGVCPSAPANRDYAGGFSSALITKDLGLASGVANASNSPIPLGSLAHKVYQSLCDKGLGNKDFSVVY 314          
BLAST of EY684612 vs. ExPASy Swiss-Prot
Match: 3HIDH_CAEEL (Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Caenorhabditis elegans GN=B0250.5 PE=2 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 2.669e-42
Identity = 85/176 (48.30%), Postives = 116/176 (65.91%), Query Frame = 2
Query:   35 LDAPVSGGVLAAEAGTLTFMVGGSEDA-YQAAKPLFLSMGKNTIYCGGAGNGAAAKICNNLTMAVSMLGVSEALTLGQSLGISASTLTKILNSSSARCWSSDSYNPVPGVMEGVPASRNYGGGFASKLMAKDLNLALASAKEVGVDCPLTSQAQDIYAKLCEN-GHDSKDFSCVFQ 556
            +DAP+SGGV  A+  TLTFMVG   DA ++ A+ +   MGKN +  G  GNG AAKICNN+ + + M+ V+E + LG S+G+ A  L  I+N+SS RCWSSD+YNPVPGV+E +P+ R Y GGF + LMAKDL+LA  ++       P+ S A  IY  L  +  + +KDF  V+Q
Sbjct:  117 IDAPISGGVTGAQQATLTFMVGAGNDATFKRAEAVLSLMGKNIVNLGAVGNGTAAKICNNMLLGIQMVAVAETMNLGISMGLDAKALAGIVNTSSGRCWSSDTYNPVPGVIENIPSCRGYAGGFGTTLMAKDLSLAQNASTNTQAPTPMGSLAHQIYRILARDPQYQAKDFGVVYQ 292          
BLAST of EY684612 vs. ExPASy Swiss-Prot
Match: MMSB_PSEAE (3-hydroxyisobutyrate dehydrogenase OS=Pseudomonas aeruginosa GN=mmsB PE=2 SV=1)

HSP 1 Score: 155.992 bits (393), Expect = 2.585e-37
Identity = 81/184 (44.02%), Postives = 115/184 (62.50%), Query Frame = 2
Query:   32 MLDAPVSGGVLAAEAGTLTFMVGGSEDAYQAAKPLFLSMGKNTIYCGGAGNGAAAKICNNLTMAVSMLGVSEALTLGQSLGISASTLTKILNSSSARCWSSDSYNPVPGVMEGVPASRNYGGGFASKLMAKDLNLALASAKEVGVDCPLTSQAQDIYA--KLCENGHDSKDFSCVFQHYYGGKD 577
            +LDAPVSGGV  A AGTL+F+VGG  + +  A+P+  +MG+N  + G  G G  AKICNN+ + + M G +EAL LG   G+  + L++++  SS   W+ + YNP PGVM   PAS  Y GGF  +LM KDL LALA+A+ V    PL + A+++++     +  H+  DFS + Q  Y GKD
Sbjct:  116 LLDAPVSGGVGGARAGTLSFIVGGPAEGFARARPVLENMGRNIFHAGDHGAGQVAKICNNMLLGILMAGTAEALALGVKNGLDPAVLSEVMKQSSGGNWALNLYNPWPGVMPQAPASNGYAGGFQVRLMNKDLGLALANAQAVQASTPLGALARNLFSLHAQADAEHEGLDFSSI-QKLYRGKD 298          
The following BLAST results are available for this feature:
BLAST of EY684612 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 32
Match NameE-valueIdentityDescription
3HIDH_ARATH2.550e-8578.53Probable 3-hydroxyisobutyrate dehydrogenase, mitoc... [more]
3HIDH_BOVIN5.733e-5358.523-hydroxyisobutyrate dehydrogenase, mitochondrial ... [more]
3HIDH_PONAB2.179e-5257.953-hydroxyisobutyrate dehydrogenase, mitochondrial ... [more]
3HIDH_HUMAN2.179e-5257.953-hydroxyisobutyrate dehydrogenase, mitochondrial ... [more]
3HIDH_MOUSE3.716e-5257.063-hydroxyisobutyrate dehydrogenase, mitochondrial ... [more]
3HIDH_RAT1.081e-5156.503-hydroxyisobutyrate dehydrogenase, mitochondrial ... [more]
3HIDH_DICDI4.701e-4754.02Probable 3-hydroxyisobutyrate dehydrogenase, mitoc... [more]
3HIDH_DROME4.400e-4551.45Probable 3-hydroxyisobutyrate dehydrogenase, mitoc... [more]
3HIDH_CAEEL2.669e-4248.30Probable 3-hydroxyisobutyrate dehydrogenase, mitoc... [more]
MMSB_PSEAE2.585e-3744.023-hydroxyisobutyrate dehydrogenase OS=Pseudomonas ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-015-C04-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY684612 ID=EY684612; Name=EY684612; organism=Citrus sinensis; type=EST; length=871bp
CCACGCGTCCGATTCATGGGAAAACCCTGTCATGTTGGATGCTCCCGTCT
CTGGGGGTGTTCTTGCTGCAGAGGCTGGTACACTAACTTTCATGGTTGGT
GGCTCTGAGGATGCATATCAGGCTGCAAAACCCTTGTTCCTCTCAATGGG
GAAAAACACAATATACTGTGGTGGAGCAGGAAATGGGGCGGCAGCAAAAA
TTTGCAATAACTTGACAATGGCTGTGAGCATGCTTGGGGTGTCAGAAGCC
CTGACACTTGGTCAGTCGCTAGGAATATCTGCTAGTACTCTAACAAAGAT
ATTGAACTCTTCAAGTGCTCGCTGTTGGAGTAGTGACAGTTATAATCCTG
TTCCTGGGGTGATGGAAGGGGTGCCGGCTTCAAGGAATTATGGTGGTGGG
TTTGCATCTAAGCTCATGGCCAAAGACTTGAACCTTGCTTTGGCATCAGC
AAAAGAGGTTGGCGTCGATTGCCCGTTAACATCACAAGCACAAGACATAT
ACGCGAAGCTCTGTGAGAATGGACATGATTCCAAGGACTTCTCCTGCGTT
TTCCAGCATTATTATGGAGGCAAGGATGAAGTTTAGCTTCAACATTCAAG
GACACTGCTTTTACCAATCCATTCTGATTCTCTTGTAAAATCAAAGCGCA
TTCAATCGAAATTAACCTCCCTAAGGCTCGTCCCTTTTCACAGAATAAAT
TGCAGCCTATAAAATCTTTTTCAGGTATATGAATATTTACCCCTGAAGAG
ATAGAGATAGAGAATCCTGGTATATGCATGTTGGAAAACCAATTAACCAA
TGGGCTTTTTCTTGGCCAAAAAAANAAAACANCNGNGCGCGTGNGTGGAA
AAAAGAAGCGAAAAAGAAAGG
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