DR908246

Overview
NameDR908246
Unique NameDR908246
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length684
Libraries
Library NameType
Citrus sinensis phloemcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DR908246 vs. ExPASy Swiss-Prot
Match: CHI1_THECC (Endochitinase 1 OS=Theobroma cacao GN=CHIA1 PE=2 SV=1)

HSP 1 Score: 312.768 bits (800), Expect = 1.097e-84
Identity = 150/233 (64.38%), Postives = 177/233 (75.97%), Query Frame = 2
Query:    2 GTAGDGGELGKIISRKMFEDLLEYRNDKRCPARCFYTYDAFIEAAKAFPAFGNSGNETMRKREIAAFFAQTGHETTGG--WPDAPGGEYAWGYCFNREVGAASSDYC--DPNYPCR-GK-YYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGYVGNDAVRNRIGFFTTFC 682
            G+ GD G L  +I+R+ F+ +L +RND  CPAR FYTYDAFI AAK+FPAF  +G++  RKRE+AAF AQT HETTGG  W  AP G Y WGYC+NRE+  A  DYC  DPNYPC  GK Y+GRGP+QLTWN NY +CG A+GVDLLNNPDLLATDP +SFKSA WFWMT Q PKPSCH+VII  W PS +D  AGR+PG+GL TNIINGG+ECG   N  V +RIGF+  +C
Sbjct:   64 GSGGDTGGLDSLITRERFDQMLLHRNDGGCPARGFYTYDAFIAAAKSFPAFATTGDDATRKREVAAFLAQTSHETTGGAGWA-APDGPYTWGYCYNRELNPA--DYCQWDPNYPCAPGKQYFGRGPMQLTWNYNYGQCGRAIGVDLLNNPDLLATDPTISFKSAFWFWMTPQSPKPSCHDVIIGAWSPSGSDQAAGRVPGFGLITNIINGGLECGQGWNAKVEDRIGFYKRYC 293          
BLAST of DR908246 vs. ExPASy Swiss-Prot
Match: CHIB_ARATH (Basic endochitinase B OS=Arabidopsis thaliana GN=CHI-B PE=1 SV=3)

HSP 1 Score: 306.99 bits (785), Expect = 6.017e-83
Identity = 141/228 (61.84%), Postives = 173/228 (75.88%), Query Frame = 2
Query:   11 GDGGELGKIISRKMFEDLLEYRNDKRCPARCFYTYDAFIEAAKAFPAFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFNREVGAASSDYCDPN--YPCRG--KYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGYVGNDAVRNRIGFFTTFC 682
            G  G+L  IIS   F+D+L++RND  CPAR FYTY+AFI AAK+FP FG +G+   RK+E+AAFF QT HETTGGW  AP G Y+WGYCF +E   AS DYC+P+  +PC    +YYGRGP+QL+WN NY  CG A+GVDLLNNPDL+A D V++FK+AIWFWMTAQPPKPSCH VI  +W+PS  D  AGRLPGYG+ TNIINGG+ECG   +  V +RIGF+  +C
Sbjct:   81 GPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPAS-DYCEPSATWPCASGKRYYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHAVIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYC 307          
BLAST of DR908246 vs. ExPASy Swiss-Prot
Match: CHI1_TOBAC (Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1 SV=2)

HSP 1 Score: 306.22 bits (783), Expect = 1.026e-82
Identity = 143/230 (62.17%), Postives = 175/230 (76.09%), Query Frame = 2
Query:    5 TAGDGGELGKIISRKMFEDLLEYRNDKRCPARCFYTYDAFIEAAKAFPAFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFNREVGAASSDYCDPN--YPCRG--KYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGYVGNDAVRNRIGFFTTFC 682
            T   GG+LG IIS  MF+ +L++RND  C  + FY+Y+AFI AA++FP FG SG+ T RKREIAAFFAQT HETTGGW  AP G YAWGYC+ RE G+   DYC P+  +PC    KY+GRGPIQ++ N NY  CG A+GVDLLNNPDL+ATDPV+SFKSA+WFWMT Q PKPSCH+VII  W+PSA D  A RLPG+G+ TNIINGG+ECG   +  V++RIGF+  +C
Sbjct:   73 TPPGGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPG-DYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGRWQPSAGDRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYC 301          
BLAST of DR908246 vs. ExPASy Swiss-Prot
Match: CHI2_BRANA (Endochitinase CH25 OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 304.679 bits (779), Expect = 2.986e-82
Identity = 139/228 (60.96%), Postives = 172/228 (75.44%), Query Frame = 2
Query:   11 GDGGELGKIISRKMFEDLLEYRNDKRCPARCFYTYDAFIEAAKAFPAFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFNREVGAASSDYCDPN--YPCRG--KYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGYVGNDAVRNRIGFFTTFC 682
            G  G+L  IISR  F+D+L++RND  CPAR FYTYDAFI AAK+FP FG +G+   RK+EIAAFF QT HETTGGW  AP G Y+WGYCF +E    SS+YC P+  +PC     YYGRGP+QL+WN NY +CG A+G DLLNNPDL++ DPV++FK+AIWFWMT Q PKPSCH VI+ +W+PS  D  AGR+PGYG+ TNIINGG+ECG   +  V +RIGF+  +C
Sbjct:   66 GPTGDLSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFPGFGTTGDTATRKKEIAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNP-SSNYCSPSAEWPCASGKSYYGRGPMQLSWNYNYGQCGRAIGSDLLNNPDLVSNDPVIAFKAAIWFWMTPQSPKPSCHAVIVGQWQPSDADRAAGRVPGYGVITNIINGGLECGRGQDARVADRIGFYQRYC 292          
BLAST of DR908246 vs. ExPASy Swiss-Prot
Match: CHI2_TOBAC (Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1 SV=1)

HSP 1 Score: 304.294 bits (778), Expect = 3.900e-82
Identity = 142/230 (61.74%), Postives = 175/230 (76.09%), Query Frame = 2
Query:    5 TAGDGGELGKIISRKMFEDLLEYRNDKRCPARCFYTYDAFIEAAKAFPAFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFNREVGAASSDYCDPN--YPCRG--KYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGYVGNDAVRNRIGFFTTFC 682
            T   GG+LG IIS  MF+ +L++RND  C  + FY+Y+AFI AA++FP FG SG+ T RKREIAAFFAQT HETTGGW  AP G YAWGYC+ RE G+   DYC P+  +PC    KY+GRGPIQ++ N NY  CG A+GVDLLNNPDL+ATDPV+SFKSA+WFWMT Q PKPSCH+VII  W+PS+ D  A RLPG+G+ TNIINGG+ECG   +  V++RIGF+  +C
Sbjct:   68 TPPGGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPG-DYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGRWQPSSADRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYC 296          
BLAST of DR908246 vs. ExPASy Swiss-Prot
Match: CHI1_GOSHI (Endochitinase 1 OS=Gossypium hirsutum PE=3 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 8.688e-82
Identity = 148/233 (63.52%), Postives = 175/233 (75.11%), Query Frame = 2
Query:    2 GTAGDGGELGKIISRKMFEDLLEYRNDKRCPARCFYTYDAFIEAAKAFPAFGNSGNETMRKREIAAFFAQTGHETTGG--WPDAPGGEYAWGYCFNREVGAASSDYC--DPNYPCR-GK-YYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGYVGNDAVRNRIGFFTTFC 682
            G A   G L  +ISR+ F  +L +RND  CPAR FYTYDAFI AA++FPAF  +G++  RKREIAAF AQT HETTGG  W  AP G YAWGYC+NRE+   SS YC  DPNYPC  GK Y+GRGP+QL+WN NY +CG A+GVDLLNNPDLL++DP +SFKSA WFWMT Q PKPSCH VII  W PS++D  AGR+PGYG+ TNIINGG+ECG   N  V +RIGF+  +C
Sbjct:   66 GPAPGPGGLTNLISRETFNQMLLHRNDGACPARGFYTYDAFIAAARSFPAFATTGDQATRKREIAAFLAQTSHETTGGAGWA-APDGPYAWGYCYNRELNPPSS-YCASDPNYPCAPGKQYFGRGPMQLSWNYNYGQCGRAIGVDLLNNPDLLSSDPTISFKSAFWFWMTPQSPKPSCHNVIIGAWSPSSSDRAAGRVPGYGVITNIINGGLECGKGWNAQVEDRIGFYKRYC 296          
BLAST of DR908246 vs. ExPASy Swiss-Prot
Match: CHI4_SOLTU (Endochitinase 4 (Fragment) OS=Solanum tuberosum GN=CHTB4 PE=2 SV=1)

HSP 1 Score: 302.753 bits (774), Expect = 1.135e-81
Identity = 142/232 (61.21%), Postives = 175/232 (75.43%), Query Frame = 2
Query:    2 GTAGDGGELGKIISRKMFEDLLEYRNDKRCPARC-FYTYDAFIEAAKAFPAFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFNREVGAASSDYCDPN--YPCRG--KYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGYVGNDAVRNRIGFFTTFC 682
            G    GG++G +IS  MF+ +L++RND  C  +  FY+Y+AFI AA++F  FG +G+ T RKREIAAFFAQT HETTGGWP AP G YAWGYCF RE G+   DYC P+  +PC    KY+GRGPIQ++ N NY  CG A+GVDLLNNPDL+ATDPV+SFKSAIWFWMT Q PKPSCH+VII  W+PSA D  A RLPG+G+ TNIINGG+ECG   +  V++RIGF+  +C
Sbjct:   70 GPGPSGGDIGSVISNSMFDQMLKHRNDNACQGKNNFYSYNAFINAARSFGGFGTTGDTTARKREIAAFFAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPG-DYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSAIWFWMTPQSPKPSCHDVIIGRWQPSAADRAANRLPGFGVITNIINGGLECGRGSDSRVQDRIGFYRRYC 300          
BLAST of DR908246 vs. ExPASy Swiss-Prot
Match: CHI9_ORYSJ (Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht9 PE=2 SV=1)

HSP 1 Score: 302.368 bits (773), Expect = 1.482e-81
Identity = 137/231 (59.31%), Postives = 175/231 (75.76%), Query Frame = 2
Query:    2 GTAGDGGELGKIISRKMFEDLLEYRNDKRCPARCFYTYDAFIEAAKAFPAFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFNREVGAASSDYCDPN--YPCRG--KYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGYVGNDAVRNRIGFFTTFC 682
            G  G GG +  ++S+++FE LL +RND  CPAR FYTYDA + AA AFP F  +G++  RKRE+AAF  QT HETTGGW  AP G Y+WGYCF  E+GA +S YC P+  +PC    KY+GRGPIQL++N NY   GEA+G DLLNNP+L+A+DPV+SFK+A+WFWMT Q PKPSCH+VI  +W PS+ D+ AGR+PGYG+ TNIINGG+ECG+  +D V NRIGF+  +C
Sbjct:   78 GGGGGGGAVEAVVSKELFEQLLLHRNDAACPARGFYTYDALVTAAAAFPDFAATGDDEARKREVAAFLGQTSHETTGGWATAPDGPYSWGYCFKEEIGATAS-YCVPSAEWPCAPDKKYFGRGPIQLSYNYNYGPAGEAIGEDLLNNPELVASDPVVSFKTALWFWMTPQSPKPSCHDVITGQWTPSSGDIAAGRVPGYGVITNIINGGLECGFGPDDRVANRIGFYQRYC 307          
BLAST of DR908246 vs. ExPASy Swiss-Prot
Match: CHIT_CARPA (Endochitinase OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 1.936e-81
Identity = 140/221 (63.35%), Postives = 171/221 (77.38%), Query Frame = 2
Query:   32 KIISRKMFEDLLEYRNDKRCPARCFYTYDAFIEAAKAFPAFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFNREVGAASSDYC--DPNYPCR-GK-YYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGYVGNDAVRNRIGFFTTFC 682
            KIISR MF+ +L++RN+  CPA+ FYTYDAF+ AAK+FP+FG +G+  +RKRE+AAF  QT HETTGGWP AP G YAWGYCF +E    SS+YC   P YPC  GK YYGRGP+QL+WN NY  CGEAL V+LL NPDL+ATD VLSFK+A+WFWMT Q PKPSCH+V+   W+PSA D  AGRLPGYG+ TN++NGG+ECG   N  V +R+GFF  +C
Sbjct:    4 KIISRSMFDQMLKHRNNPACPAKGFYTYDAFLAAAKSFPSFGTTGSTDVRKRELAAFLGQTSHETTGGWPSAPDGPYAWGYCFLKERNP-SSNYCAPSPRYPCAPGKSYYGRGPLQLSWNYNYGPCGEALRVNLLGNPDLVATDRVLSFKTALWFWMTPQAPKPSCHDVLTGRWQPSAADTAAGRLPGYGVLTNLLNGGLECGKGPNPQVADRLGFFRRYC 223          
BLAST of DR908246 vs. ExPASy Swiss-Prot
Match: CHIT_SOLTU (Endochitinase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 299.671 bits (766), Expect = 9.606e-81
Identity = 139/228 (60.96%), Postives = 172/228 (75.44%), Query Frame = 2
Query:   11 GDGGELGKIISRKMFEDLLEYRNDKRCPARCFYTYDAFIEAAKAFPAFGNSGNETMRKREIAAFFAQTGHETTGGWPDAPGGEYAWGYCFNREVGAASSDYCDPN--YPCRG--KYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIINGGIECGYVGNDAVRNRIGFFTTFC 682
            G GG+LG  IS  MF+ +L++RN+  C  + FY+Y+AFI AA++FP FG SG+   RKREIAAFFAQT HETTGGW  AP G YAWGYCF RE G    DYC P+  +PC    KY+GRGPIQ++ N NY  CG A+GVDLLNNPDL+ATDPV+SFK+A+WFWMT Q PKPSCH+VII  W PS+ D  A RLPG+G+ TNIINGG+ECG   ++ V++RIGF+  +C
Sbjct:   74 GPGGDLGSAISNSMFDQMLKHRNENSCQGKNFYSYNAFINAARSFPGFGTSGDINARKREIAAFFAQTSHETTGGWASAPDGPYAWGYCFLRERGNPG-DYCPPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHDVIIGRWNPSSADRAANRLPGFGVITNIINGGLECGRGTDNRVQDRIGFYRRYC 300          
The following BLAST results are available for this feature:
BLAST of DR908246 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 56
Match NameE-valueIdentityDescription
CHI1_THECC1.097e-8464.38Endochitinase 1 OS=Theobroma cacao GN=CHIA1 PE=2 S... [more]
CHIB_ARATH6.017e-8361.84Basic endochitinase B OS=Arabidopsis thaliana GN=C... [more]
CHI1_TOBAC1.026e-8262.17Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1... [more]
CHI2_BRANA2.986e-8260.96Endochitinase CH25 OS=Brassica napus PE=2 SV=1[more]
CHI2_TOBAC3.900e-8261.74Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1... [more]
CHI1_GOSHI8.688e-8263.52Endochitinase 1 OS=Gossypium hirsutum PE=3 SV=1[more]
CHI4_SOLTU1.135e-8161.21Endochitinase 4 (Fragment) OS=Solanum tuberosum GN... [more]
CHI9_ORYSJ1.482e-8159.31Chitinase 9 OS=Oryza sativa subsp. japonica GN=Cht... [more]
CHIT_CARPA1.936e-8163.35Endochitinase OS=Carica papaya PE=1 SV=1[more]
CHIT_SOLTU9.606e-8160.96Endochitinase OS=Solanum tuberosum PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_16374 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-33_E04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR908246 ID=DR908246; Name=DR908246; organism=Citrus sinensis; type=EST; length=684bp
CGGGACGGCTGGCGATGGCGGTGAACTGGGCAAGATCATCTCAAGGAAGA
TGTTTGAAGACTTGCTTGAGTATAGAAATGATAAACGATGCCCTGCCAGA
TGCTTCTACACTTATGATGCTTTTATAGAGGCAGCCAAAGCTTTTCCAGC
CTTTGGTAATTCTGGAAATGAAACCATGCGTAAAAGAGAAATTGCTGCCT
TTTTTGCCCAAACTGGCCATGAAACTACTGGGGGATGGCCCGATGCACCT
GGTGGCGAATATGCTTGGGGATATTGCTTTAATAGGGAAGTTGGTGCTGC
GTCGTCCGACTACTGTGATCCTAACTATCCATGTCGAGGGAAGTACTATG
GCCGAGGTCCAATTCAACTCACTTGGAATTGCAATTACTTAAGGTGCGGA
GAAGCCTTGGGGGTGGACTTGTTGAACAATCCAGACCTACTTGCCACTGA
TCCAGTACTATCATTCAAGTCAGCAATCTGGTTCTGGATGACTGCACAGC
CACCAAAGCCATCGTGCCACGAAGTCATTATCGACGAATGGAAACCATCA
GCAAATGACGTAAACGCCGGCCGGCTTCCGGGATACGGTCTAACCACAAA
TATAATCAACGGTGGAATTGAATGTGGCTACGTTGGGAATGACGCTGTAC
GTAACCGTATTGGGTTCTTTACCACTTTCTGTGG
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