DC887599

Overview
NameDC887599
Unique NameDC887599
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length680
Libraries
Library NameType
EICcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig6299 contig Csv1_Contig6299:521..1199. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DC887599 vs. ExPASy Swiss-Prot
Match: IDI2_ARATH (Isopentenyl-diphosphate Delta-isomerase II OS=Arabidopsis thaliana GN=IPP2 PE=2 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 1.720e-60
Identity = 94/106 (88.68%), Postives = 100/106 (94.34%), Query Frame = -3 
Query:  271 DEFTPLGRILYKAPSDGKWGEHELDYLLFIVRDVSVNPNPDEVAEYKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHLEKGTLNEVIDMKTIHKL 588
            DEFTPLGR+LYKAPSDGKWGEHELDYLLFIVRDV V PNPDEVAE KYV+RE+LKEL++KADAGEEGLKLSPWFRLVVDNFL KWWDH+EKGTL E IDMKTIHKL
Sbjct:  179 DEFTPLGRMLYKAPSDGKWGEHELDYLLFIVRDVKVQPNPDEVAEIKYVSREELKELVKKADAGEEGLKLSPWFRLVVDNFLMKWWDHVEKGTLVEAIDMKTIHKL 284

HSP 2 Score: 49.6766 bits (117), Expect = 1.720e-60
Identity = 24/27 (88.89%), Postives = 26/27 (96.30%), Query Frame = -2 
Query:  599 LIEENALGVRNAAQRKLLDELGICAED 679
            LI++NALGVRNAAQRKLLDELGI AED
Sbjct:  149 LIQDNALGVRNAAQRKLLDELGIVAED 175
[Close]
BLAST of DC887599 vs. ExPASy Swiss-Prot
Match: IDI1_CAMAC (Isopentenyl-diphosphate Delta-isomerase I OS=Camptotheca acuminata GN=IPI1 PE=2 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 2.929e-60
Identity = 93/106 (87.74%), Postives = 101/106 (95.28%), Query Frame = -3 
Query:  271 DEFTPLGRILYKAPSDGKWGEHELDYLLFIVRDVSVNPNPDEVAEYKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHLEKGTLNEVIDMKTIHKL 588
            D+F PLGRILYKAPSDGKWGEHELDYLLFIVRDV+VNPNPDEVA+ KYV R+QLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDH+EKGT++E  DMK+IHKL
Sbjct:  129 DQFIPLGRILYKAPSDGKWGEHELDYLLFIVRDVNVNPNPDEVADIKYVTRDQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHVEKGTMHEAADMKSIHKL 234

HSP 2 Score: 48.1358 bits (113), Expect = 2.929e-60
Identity = 24/27 (88.89%), Postives = 25/27 (92.59%), Query Frame = -2 
Query:  599 LIEENALGVRNAAQRKLLDELGICAED 679
            LI+ENALG RNAAQRKLLDELGI AED
Sbjct:   99 LIKENALGARNAAQRKLLDELGIPAED 125
[Close]
BLAST of DC887599 vs. ExPASy Swiss-Prot
Match: IDI2_CLABR (Isopentenyl-diphosphate Delta-isomerase II OS=Clarkia breweri GN=IPI2 PE=3 SV=2)

HSP 1 Score: 203.756 bits (517), Expect = 3.812e-60
Identity = 94/106 (88.68%), Postives = 102/106 (96.23%), Query Frame = -3 
Query:  271 DEFTPLGRILYKAPSDGKWGEHELDYLLFIVRDVSVNPNPDEVAEYKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHLEKGTLNEVIDMKTIHKL 588
            DEFTPL R+LYKAPSDGKWGEHELDYLLFIVRDV+V+PNPDEVAE KYVNREQLKELL+KADAGEEGLKLSPWFRLVVDNFL KWWDH+EKGTL+E +DMK+IHKL
Sbjct:  181 DEFTPLSRMLYKAPSDGKWGEHELDYLLFIVRDVAVHPNPDEVAEIKYVNREQLKELLKKADAGEEGLKLSPWFRLVVDNFLPKWWDHVEKGTLSEAVDMKSIHKL 286

HSP 2 Score: 47.7506 bits (112), Expect = 3.812e-60
Identity = 24/27 (88.89%), Postives = 25/27 (92.59%), Query Frame = -2 
Query:  599 LIEENALGVRNAAQRKLLDELGICAED 679
            LI+EN LGVRNAAQRKLLDELGI AED
Sbjct:  151 LIDENNLGVRNAAQRKLLDELGIPAED 177
[Close]
BLAST of DC887599 vs. ExPASy Swiss-Prot
Match: IDI2_CAMAC (Isopentenyl-diphosphate Delta-isomerase II OS=Camptotheca acuminata GN=IPI2 PE=2 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 1.871e-59
Identity = 89/107 (83.18%), Postives = 101/107 (94.39%), Query Frame = -3 
Query:  268 DEFTPLGRILYKAPSDGKWGEHELDYLLFIVRDVSVNPNPDEVAEYKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHLEKGTLNEVIDMKTIHKLT 588
            D+F PLGR+LYKAPSDGKWGEHELDYLLFI+RDV+V+PNPDEVA+ KYVN++QLK+LLRK DAGEEGLKLSPWFRLVV+NFLFKWWDH+EKGTL +  DMKTIHKLT
Sbjct:  203 DQFIPLGRMLYKAPSDGKWGEHELDYLLFIIRDVNVHPNPDEVADVKYVNQDQLKDLLRKVDAGEEGLKLSPWFRLVVENFLFKWWDHVEKGTLQDATDMKTIHKLT 309

HSP 2 Score: 49.6766 bits (117), Expect = 1.871e-59
Identity = 24/27 (88.89%), Postives = 26/27 (96.30%), Query Frame = -2 
Query:  599 LIEENALGVRNAAQRKLLDELGICAED 679
            L++ENALGVRNAAQRKLLDELGI AED
Sbjct:  173 LVDENALGVRNAAQRKLLDELGIPAED 199
[Close]
BLAST of DC887599 vs. ExPASy Swiss-Prot
Match: IDI2_CLAXA (Isopentenyl-diphosphate Delta-isomerase II OS=Clarkia xantiana GN=IPI2 PE=3 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 1.872e-59
Identity = 92/106 (86.79%), Postives = 100/106 (94.34%), Query Frame = -3 
Query:  271 DEFTPLGRILYKAPSDGKWGEHELDYLLFIVRDVSVNPNPDEVAEYKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHLEKGTLNEVIDMKTIHKL 588
            DEFTP+ R+LYKAPSDGKWGEHELDYLLFIVRDV ++PNPDEVA+ KYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFL KWWDH+EKGTL+E +DMKTIH L
Sbjct:  185 DEFTPVSRMLYKAPSDGKWGEHELDYLLFIVRDVGIHPNPDEVADIKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLPKWWDHVEKGTLSEAVDMKTIHNL 290

HSP 2 Score: 47.7506 bits (112), Expect = 1.872e-59
Identity = 24/27 (88.89%), Postives = 25/27 (92.59%), Query Frame = -2 
Query:  599 LIEENALGVRNAAQRKLLDELGICAED 679
            LI+EN LGVRNAAQRKLLDELGI AED
Sbjct:  155 LIDENNLGVRNAAQRKLLDELGIPAED 181
[Close]
BLAST of DC887599 vs. ExPASy Swiss-Prot
Match: IDI1_ARATH (Isopentenyl-diphosphate Delta-isomerase I OS=Arabidopsis thaliana GN=IPP1 PE=2 SV=2)

HSP 1 Score: 194.512 bits (493), Expect = 1.003e-57
Identity = 87/106 (82.08%), Postives = 98/106 (92.45%), Query Frame = -3 
Query:  271 DEFTPLGRILYKAPSDGKWGEHELDYLLFIVRDVSVNPNPDEVAEYKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHLEKGTLNEVIDMKTIHKL 588
            DEFTPLGR+LYKAPSDGKWGEHE+DYLLFIVRDV + PNPDEVAE KYV+RE+LKEL++KADAG+E +KLSPWFRLVVDNFL KWWDH+EKGT+ E  DMKTIHKL
Sbjct:  128 DEFTPLGRMLYKAPSDGKWGEHEVDYLLFIVRDVKLQPNPDEVAEIKYVSREELKELVKKADAGDEAVKLSPWFRLVVDNFLMKWWDHVEKGTITEAADMKTIHKL 233

HSP 2 Score: 48.9062 bits (115), Expect = 1.003e-57
Identity = 24/27 (88.89%), Postives = 24/27 (88.89%), Query Frame = -2 
Query:  599 LIEENALGVRNAAQRKLLDELGICAED 679
            LIEEN LGVRNAAQRKL DELGI AED
Sbjct:   98 LIEENVLGVRNAAQRKLFDELGIVAED 124
[Close]
BLAST of DC887599 vs. ExPASy Swiss-Prot
Match: IDI1_CLABR (Isopentenyl-diphosphate Delta-isomerase I OS=Clarkia breweri GN=IPI1 PE=2 SV=2)

HSP 1 Score: 193.356 bits (490), Expect = 2.893e-57
Identity = 87/106 (82.08%), Postives = 98/106 (92.45%), Query Frame = -3 
Query:  271 DEFTPLGRILYKAPSDGKWGEHELDYLLFIVRDVSVNPNPDEVAEYKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHLEKGTLNEVIDMKTIHKL 588
            D+F PL RILYKAPSDGKWGEHELDYLLFI+RDV+++PNPDEVAE KY+NR+ LKELLRKADA EEG+KLSPWFRLVVDNFLFKWWDH+EKG+L +  DMKTIHKL
Sbjct:  182 DQFIPLSRILYKAPSDGKWGEHELDYLLFIIRDVNLDPNPDEVAEVKYMNRDDLKELLRKADAEEEGVKLSPWFRLVVDNFLFKWWDHVEKGSLKDAADMKTIHKL 287

HSP 2 Score: 48.521 bits (114), Expect = 2.893e-57
Identity = 24/27 (88.89%), Postives = 25/27 (92.59%), Query Frame = -2 
Query:  599 LIEENALGVRNAAQRKLLDELGICAED 679
            LI+EN LGVRNAAQRKLLDELGI AED
Sbjct:  152 LIDENCLGVRNAAQRKLLDELGIPAED 178
[Close]
BLAST of DC887599 vs. ExPASy Swiss-Prot
Match: IDI1_MESAU (Isopentenyl-diphosphate Delta-isomerase 1 OS=Mesocricetus auratus GN=IDI1 PE=2 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.272e-31
Identity = 55/106 (51.89%), Postives = 84/106 (79.25%), Query Frame = -3 
Query:  271 DEFTPLGRILYKAPSDGKWGEHELDYLLFIVRDVSVNPNPDEVAEYKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHLEKGTLNEVIDMKTIHKL 588
            +E   L RI YKA SDG WGEHE+DY+LF+ ++V++NP+P+E+  Y YV++E+LKEL++KA +GE  +KL+PWF+++VD FLFKWWD+L    L++ +D + IH++
Sbjct:  126 NEMHYLTRIYYKAQSDGIWGEHEIDYILFLKKNVTLNPDPNEIKSYCYVSKEELKELVKKAASGE--VKLTPWFKIIVDTFLFKWWDNLNH--LSQFVDHEKIHRM 227

HSP 2 Score: 31.187 bits (69), Expect = 1.272e-31
Identity = 16/34 (47.06%), Postives = 22/34 (64.71%), Query Frame = -2 
Query:  578 LIEENALGVRNAAQRKLLDELGICAEDXXXXXVH 679
            L E +A+GV+ AAQR+L  ELGI  E+     +H
Sbjct:   96 LEENDAIGVKRAAQRRLKAELGIPLEEVDPNEMH 129
[Close]
BLAST of DC887599 vs. ExPASy Swiss-Prot
Match: IDI1_MACFA (Isopentenyl-diphosphate Delta-isomerase 1 OS=Macaca fascicularis GN=IDI1 PE=2 SV=2)

HSP 1 Score: 121.709 bits (304), Expect = 4.764e-31
Identity = 53/106 (50.00%), Postives = 82/106 (77.36%), Query Frame = -3 
Query:  271 DEFTPLGRILYKAPSDGKWGEHELDYLLFIVRDVSVNPNPDEVAEYKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHLEKGTLNEVIDMKTIHKL 588
            +E   L RI YKA SDG WGEHE+DY+LF+ ++V++NP+P+E+  + YV++E+LKELL+KA  GE  +K++PWF+++ + FLFKWWD+L    LN+ +D + IH++
Sbjct:  126 EEINYLTRIHYKAQSDGIWGEHEIDYILFVRKNVTLNPDPNEIKSFCYVSKEELKELLKKAANGE--IKITPWFQIIAETFLFKWWDNLNH--LNQFVDHEKIHRM 227

HSP 2 Score: 32.3426 bits (72), Expect = 4.764e-31
Identity = 17/27 (62.96%), Postives = 20/27 (74.07%), Query Frame = -2 
Query:  599 LIEENALGVRNAAQRKLLDELGICAED 679
            L E +ALGVR AAQR+L  ELGI  E+
Sbjct:   96 LEENDALGVRRAAQRRLKAELGIPLEE 122
[Close]
BLAST of DC887599 vs. ExPASy Swiss-Prot
Match: IDI1_MOUSE (Isopentenyl-diphosphate Delta-isomerase 1 OS=Mus musculus GN=Idi1 PE=2 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 1.370e-30
Identity = 54/112 (48.21%), Postives = 84/112 (75.00%), Query Frame = -3 
Query:  271 LKMCQFDEFTPLGRILYKAPSDGKWGEHELDYLLFIVRDVSVNPNPDEVAEYKYVNREQLKELLRKADAGEEGLKLSPWFRLVVDNFLFKWWDHLEKGTLNEVIDMKTIHKL 606
            L+    +E   L RI YKA SDG WGEHE+DY+LF+ ++V++NP+P+E+  Y YV++E+++E+L+KA +GE  +KL+PWF+++ D FLFKWWD+L    L+  +D + IH+L
Sbjct:  120 LEEVDLNEMDYLTRIYYKAQSDGIWGEHEVDYILFLRKNVTLNPDPNEIKSYCYVSKEEVREILKKAASGE--IKLTPWFKIIADTFLFKWWDNLNH--LSPFVDHEKIHRL 227

HSP 2 Score: 30.8018 bits (68), Expect = 1.370e-30
Identity = 15/27 (55.56%), Postives = 20/27 (74.07%), Query Frame = -2 
Query:  599 LIEENALGVRNAAQRKLLDELGICAED 679
            L E NA+GV+ AA+R+L  ELGI  E+
Sbjct:   96 LEENNAIGVKRAAKRRLKAELGIPLEE 122
The following BLAST results are available for this feature:
BLAST of DC887599 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 19
Match NameE-valueIdentityDescription
IDI2_ARATH1.720e-6088.68Isopentenyl-diphosphate Delta-isomerase II OS=Arab... [more]
IDI1_CAMAC2.929e-6087.74Isopentenyl-diphosphate Delta-isomerase I OS=Campt... [more]
IDI2_CLABR3.812e-6088.68Isopentenyl-diphosphate Delta-isomerase II OS=Clar... [more]
IDI2_CAMAC1.871e-5983.18Isopentenyl-diphosphate Delta-isomerase II OS=Camp... [more]
IDI2_CLAXA1.872e-5986.79Isopentenyl-diphosphate Delta-isomerase II OS=Clar... [more]
IDI1_ARATH1.003e-5782.08Isopentenyl-diphosphate Delta-isomerase I OS=Arabi... [more]
IDI1_CLABR2.893e-5782.08Isopentenyl-diphosphate Delta-isomerase I OS=Clark... [more]
IDI1_MESAU1.272e-3151.89Isopentenyl-diphosphate Delta-isomerase 1 OS=Mesoc... [more]
IDI1_MACFA4.764e-3150.00Isopentenyl-diphosphate Delta-isomerase 1 OS=Macac... [more]
IDI1_MOUSE1.370e-3048.21Isopentenyl-diphosphate Delta-isomerase 1 OS=Mus m... [more]

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Properties
Property NameValue
Genbank descriptionDC887599 EIC Citrus sinensis cDNA clone EIC0613 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DC887599 ID=DC887599; Name=DC887599; organism=Citrus sinensis; type=EST; length=680bp
CCCCCCCCCCCCGNTATGTAATGTCAAAATTACAAACTTAACAATAAAAT
TCCATAAAAGCAGCACATACNAAAAGATAAGGTAACGTCAACAACAAAAT
GCTAATCTGTGTAGAACTCTCGCATATCCAAATTTATTGGAATAAAGAAA
CCGGTGAGAAGATACTGTGTACCCTTAATGTCTTGAAGAACTCTCAAATG
CCCAAACTTATTGGAATACAGGTTCCGGCACACAAATACTGTGTTTCAAG
GGCCTGCGGCTTTATTAAGTCAACTTGTGAATGGTCTTCATGTCAATGAC
TTCGTTAAGGGTACCTTTTTCGAGGTGATCCCACCACTTGAACAAGAAAT
TGTCCACAACCAGTCTGAACCAAGGAGACAGCTTCAAACCTTCTTCTCCA
GCATCTGCTTTCCTCAAAAGCTCTTTTAACTGTTCCCGGTTGACATATTT
ATACTCGGCTACTTCATCAGGATTTGGGTTAACACTAACATCTCGGACAA
TGAAAAGTAAGTAGTCAAGTTCATGCTCCCCCCACTTGCCATCAGAAGGG
GCCTTGTACAGAATGCGACCCAGTGGAGTGAACTCATCAAACTGGCACAT
CTTCAGCACAAATGCCCAGCTCATCCAAAAGCTTTCTTTGTGCAGCATTC
CTCACCCCAAGAGCGTTCTCCTCAATAAGC
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