CN189031

Overview
NameCN189031
Unique NameCN189031
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length688
Libraries
Library NameType
Washington Navel Orange Stored Fruit Rind cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN189031 vs. ExPASy Swiss-Prot
Match: RD21A_ARATH (Cysteine proteinase RD21a OS=Arabidopsis thaliana GN=RD21A PE=1 SV=1)

HSP 1 Score: 287.73 bits (735), Expect = 3.811e-77
Identity = 142/211 (67.30%), Postives = 172/211 (81.52%), Query Frame = 3
Query:   69 FRSPTMAMFLLFLFTVSSALDMSIVSYDKTHESKSSSWRTDDEVMAMFEAWLVKHGKAY--NALGEKEKRFEIFKENLRFIDEHNSENRTYKVGLNRFADLTNEEYRSMHLGARF---GAKRTSLKSKRSNRYLPRVGEALPDSVDWRKKGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVTGSLISLSEQELVDCDTSYNEGCNGGLMDY 686
            F  PTMA+  L +  VSSA+DMSI+SYD+ H   ++  R++ EVM+++EAWLVKHGKA   N+L EK++RFEIFK+NLRF+DEHN +N +Y++GL RFADLTN+EYRS +LGA+    G +RTSL      RY  RVG+ LP+S+DWRKKGAVAEVKDQG CGSCWAFSTI AVEGIN+IVTG LI+LSEQELVDCDTSYNEGCNGGLMDY
Sbjct:    3 FLKPTMAILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGERRTSL------RYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITLSEQELVDCDTSYNEGCNGGLMDY 207          
BLAST of CN189031 vs. ExPASy Swiss-Prot
Match: ORYA_ORYSJ (Oryzain alpha chain OS=Oryza sativa subsp. japonica GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 241.506 bits (615), Expect = 3.132e-63
Identity = 123/208 (59.13%), Postives = 152/208 (73.08%), Query Frame = 3
Query:   75 SPTMAMFLLFLFTVSSALDMSIVSYDKTHESKSSSWRTDDEVMAMFEAWLVKHGKAYNALGEKEKRFEIFKENLRFIDEHNSEN----RTYKVGLNRFADLTNEEYRSMHLGARFGAKRTSLKSKRSNRYLPRVGEALPDSVDWRKKGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVTGSLISLSEQELVDCDTSYNEGCNGGLMDY 686
            S  +A   L L    +A DMSIVSY +         R+++E   ++  W  +HGK+YNA+GE+E+R+  F++NLR+IDEHN+       ++++GLNRFADLTNEEYR  +LG R   +R   + K S+RYL    EALP+SVDWR KGAVAE+KDQG CGSCWAFS IAAVEGIN+IVTG LISLSEQELVDCDTSYNEGCNGGLMDY
Sbjct:    4 SMALAAAALLLLLSLAAADMSIVSYGE---------RSEEEARRLYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEHNAAADAGVHSFRLGLNRFADLTNEEYRDTYLGLRNKPRR---ERKVSDRYLAADNEALPESVDWRTKGAVAEIKDQGGCGSCWAFSAIAAVEGINQIVTGDLISLSEQELVDCDTSYNEGCNGGLMDY 199          
BLAST of CN189031 vs. ExPASy Swiss-Prot
Match: ACTN_ACTDE (Actinidain OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 2.481e-60
Identity = 124/207 (59.90%), Postives = 155/207 (74.88%), Query Frame = 3
Query:   60 MGLFRSPTMAMFLLFLFTVSSALDMSIVSYDKTHESKSSSWRTDDEVMAMFEAWLVKHGKAYNALGEKEKRFEIFKENLRFIDEHNSE-NRTYKVGLNRFADLTNEEYRSMHLGARFGAKRTSLKSKRSNRYLPRVGEALPDSVDWRKKGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVTGSLISLSEQELVDCDTSYN-EGCNGG 674
            MGL +S  ++M LLF  T+   L +S+        +K+ + RT+DEV AM+E+WL+K+GK+YN+LGE E+RFEIFKE LRFIDEHN++ NR+YKVGLN+FADLT+EE+RS +LG   G+ +T    K SNRY PRVG+ LP  VDWR  GAV ++K QG CG CWAFS IA VEGINKIVTG LISLSEQEL+DC  + N  GCNGG
Sbjct:    1 MGLPKS-FVSMSLLFFSTL---LILSLA-----FNAKNLTQRTNDEVKAMYESWLIKYGKSYNSLGEWERRFEIFKETLRFIDEHNADTNRSYKVGLNQFADLTDEEFRSTYLGFTSGSNKT----KVSNRYEPRVGQVLPSYVDWRSAGAVVDIKSQGECGGCWAFSAIATVEGINKIVTGVLISLSEQELIDCGRTQNTRGCNGG 194          
BLAST of CN189031 vs. ExPASy Swiss-Prot
Match: ACTN_ACTCH (Actinidain OS=Actinidia chinensis PE=1 SV=4)

HSP 1 Score: 228.794 bits (582), Expect = 2.101e-59
Identity = 123/207 (59.42%), Postives = 154/207 (74.40%), Query Frame = 3
Query:   60 MGLFRSPTMAMFLLFLFTVSSALDMSIVSYDKTHESKSSSWRTDDEVMAMFEAWLVKHGKAYNALGEKEKRFEIFKENLRFIDEHNSE-NRTYKVGLNRFADLTNEEYRSMHLGARFGAKRTSLKSKRSNRYLPRVGEALPDSVDWRKKGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVTGSLISLSEQELVDCDTSYN-EGCNGG 674
            MGL +S  ++M LLF  T+   L +S+        +K+ + RT+DEV AM+E+WL+K+GK+YN+LGE E+RFEIFKE LRFIDEHN++ NR+YKVGLN+FADLT+EE+RS +L    G+ +T    K SNRY PRVG+ LP  VDWR  GAV ++K QG CG CWAFS IA VEGINKIVTG LISLSEQEL+DC  + N  GCNGG
Sbjct:    1 MGLPKS-FVSMSLLFFSTL---LILSLA-----FNAKNLTQRTNDEVKAMYESWLIKYGKSYNSLGEWERRFEIFKETLRFIDEHNADTNRSYKVGLNQFADLTDEEFRSTYLRFTSGSNKT----KVSNRYEPRVGQVLPSYVDWRSAGAVVDIKSQGECGGCWAFSAIATVEGINKIVTGVLISLSEQELIDCGRTQNTRGCNGG 194          
BLAST of CN189031 vs. ExPASy Swiss-Prot
Match: GCP1_ARATH (Germination-specific cysteine protease 1 OS=Arabidopsis thaliana GN=GCP1 PE=2 SV=2)

HSP 1 Score: 227.639 bits (579), Expect = 4.680e-59
Identity = 115/209 (55.02%), Postives = 151/209 (72.25%), Query Frame = 3
Query:   84 MAMFLLFLFTVSSALDMSIVSYDKTHESKSSSWRTDDEVMAMFEAWLVKHGKAYN----ALGEKEKRFEIFKENLRFIDEHNSENR--TYKVGLNRFADLTNEEYRSMHLGARFGAKRTSLKSKRSN-RYLPRV-GEALPDSVDWRKKGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVTGSLISLSEQELVDCDTSYNEGCNGGLMDY 686
            +++ LL++    ++ D SI++ D         WRTD+EV +++  W  +HGK  N     + +++KRF IFK+NLRFID HN +N+  TYK+GL +F DLTN+EYR ++LGAR    R   K+K  N +Y   V G+ +P++VDWR+KGAV  +KDQG+CGSCWAFST AAVEGINKIVTG LISLSEQELVDCD SYN+GCNGGLMDY
Sbjct:    8 LSLLLLYVVVSLASGDESIIN-DHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNNNNNGIINDQDKRFNIFKDNLRFIDLHNEDNKNATYKLGLTKFTDLTNDEYRKLYLGARTEPARRIAKAKNVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGINKIVTGELISLSEQELVDCDKSYNQGCNGGLMDY 215          
BLAST of CN189031 vs. ExPASy Swiss-Prot
Match: CPR1_ARATH (Probable cysteine proteinase At3g19400 OS=Arabidopsis thaliana GN=At3g19400 PE=2 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 2.403e-55
Identity = 108/202 (53.47%), Postives = 148/202 (73.27%), Query Frame = 3
Query:   87 AMFLLFLFTVSSALDMSIVSYDKTHESKSSSWRTDDEVMAMFEAWLVKHGKAYNALGEKEKRFEIFKENLRFIDEHNS-ENRTYKVGLNRFADLTNEEYRSMHLGARFGAKRTSLKSKRSNRYLPRVGEALPDSVDWRKKGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVTGSLISLSEQELVDCDTSY-NEGCNGGLMDY 686
            A+ +L +  +SS+L ++         +++   R + EV  M+E WLV++ K YN LGEKE+RF+IFK+NL+F+DEHNS  +RT++VGL RFADLTNEE+R+++L  +    + S+K++R   YL + G+ LPD VDWR  GAV  VKDQG+CGSCWAFS + AVEGIN+I TG LISLSEQELVDCD  + N GC+GG+M+Y
Sbjct:   12 ALVILSVLLLSSSLGVA---------TETEIERNETEVRLMYEQWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRTFEVGLTRFADLTNEEFRAIYLRKKMERTKDSVKTER---YLYKEGDVLPDEVDWRANGAVVSVKDQGNCGSCWAFSAVGAVEGINQITTGELISLSEQELVDCDRGFVNAGCDGGIMNY 201          
BLAST of CN189031 vs. ExPASy Swiss-Prot
Match: XCP1_ARATH (Xylem cysteine proteinase 1 OS=Arabidopsis thaliana GN=XCP1 PE=1 SV=1)

HSP 1 Score: 213.772 bits (543), Expect = 6.993e-55
Identity = 110/213 (51.64%), Postives = 146/213 (68.54%), Query Frame = 3
Query:   69 FRSPTMAMFLLFLFTVSSAL-------DMSIVSYDKTHESKSSSWRTDDEVMAMFEAWLVKHGKAYNALGEKEKRFEIFKENLRFIDEHNSENRTYKVGLNRFADLTNEEYRSMHLGARFGAKRTSLKSKRSNRYLPRVGEALPDSVDWRKKGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVTGSLISLSEQELVDCDTSYNEGCNGGLMDY 686
            F +P+++ F L +   +SAL       D SIV Y   H + +      D+++ +FE+W+ +H KAY ++ EK  RFE+F+ENL  ID+ N+E  +Y +GLN FADLT+EE++  +LG      + S K + S  +  R    LP SVDWRKKGAVA VKDQG CGSCWAFST+AAVEGIN+I TG+L SLSEQEL+DCDT++N GCNGGLMDY
Sbjct:    3 FSAPSLSKFSLLVAISASALLCCAFARDFSIVGYTPEHLTNT------DKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGRYLG--LAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCDTTFNSGCNGGLMDY 207          
BLAST of CN189031 vs. ExPASy Swiss-Prot
Match: XCP2_ARATH (Xylem cysteine proteinase 2 OS=Arabidopsis thaliana GN=XCP2 PE=1 SV=2)

HSP 1 Score: 208.764 bits (530), Expect = 2.250e-53
Identity = 104/190 (54.74%), Postives = 136/190 (71.58%), Query Frame = 3
Query:  117 SSALDMSIVSYDKTHESKSSSWRTDDEVMAMFEAWLVKHGKAYNALGEKEKRFEIFKENLRFIDEHNSENRTYKVGLNRFADLTNEEYRSMHLGARFGAKRTSLKSKRSNRYLPRVGEALPDSVDWRKKGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVTGSLISLSEQELVDCDTSYNEGCNGGLMDY 686
            +S+ D SIV Y            + D+++ +FE W+    KAY  + EK  RFE+FK+NL+ IDE N + ++Y +GLN FADL++EE++ M+LG +    R   + +    +  R  EA+P SVDWRKKGAVAEVK+QGSCGSCWAFST+AAVEGINKIVTG+L +LSEQEL+DCDT+YN GCNGGLMDY
Sbjct:   26 ASSHDYSIVGYSP------EDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFKKMYLGLKTDIVRRD-EERSYAEFAYRDVEAVPKSVDWRKKGAVAEVKNQGSCGSCWAFSTVAAVEGINKIVTGNLTTLSEQELIDCDTTYNNGCNGGLMDY 208          
BLAST of CN189031 vs. ExPASy Swiss-Prot
Match: CPR4_ARATH (Probable cysteine proteinase At4g11320 OS=Arabidopsis thaliana GN=At4g11320 PE=2 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 9.451e-52
Identity = 108/214 (50.47%), Postives = 142/214 (66.36%), Query Frame = 3
Query:   60 MGLFRSPTMAMFLLFLFTVS--SALDMSIVSYDKTHESKSSSWRT----DDEVMAMFEAWLVKHGKAYNALGEKEKRFEIFKENLRFIDEHNSENRTYKVGLNRFADLTNEEYRSMHLGARFGAKRTSLKSKRSNRYLPRVGEALPDSVDWRKKGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVTGSLISLSEQELVDCDTSYNEGCNGGLMD 683
            MG  +S  M +FLL L   S  +A+DMS+VS +  H   +   R     D E   MFE+W+VKHGK Y+++ EKE+R  IF++NLRFI   N+EN +Y++GLNRFADL+  EY  +  GA     R  +    SNRY    G+ LP SVDWR +GAV EVKDQG C SCWAFST+ AVEG+NKIVTG L++LSEQ+L++C+   N GC GG ++
Sbjct:    1 MGYAKS-AMLIFLLALVIASCATAMDMSVVSSNDNHHVTAGPGRRQGIFDAEATLMFESWMVKHGKVYDSVAEKERRLTIFEDNLRFITNRNAENLSYRLGLNRFADLSLHEYGEICHGADPRPPRNHVFMTSSNRYKTSDGDVLPKSVDWRNEGAVTEVKDQGLCRSCWAFSTVGAVEGLNKIVTGELVTLSEQDLINCNKE-NNGCGGGKVE 212          
BLAST of CN189031 vs. ExPASy Swiss-Prot
Match: CYSP4_BRANA (Cysteine proteinase COT44 (Fragment) OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 4.691e-51
Identity = 101/170 (59.41%), Postives = 130/170 (76.47%), Query Frame = 3
Query:  201 MAMFEAWLVKHGKAYN----ALGEKEKRFEIFKENLRFIDEHNSENR--TYKVGLNRFADLTNEEYRSMHLGARFGAKRTSLKSKRSN-RYLPRVG-EALPDSVDWRKKGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVTGSLISLSEQELVDCDTSYNEGCNGGLMDY 686
            M+++  W ++HGK+ +     + ++++RF IFK+NLRFID HN  N+  TYK+GL  FA+LTN+EYRS++LGAR    R   K+K  N +Y   V  + +P +VDWR+KGAV  +KDQG+CGSCWAFST AAVEGINKIVTG L+SLSEQELVDCD SYN+GCNGGLMDY
Sbjct:    1 MSIYLRWSLEHGKSNSNSNGIINQQDERFNIFKDNLRFIDLHNENNKNATYKLGLTIFANLTNDEYRSLYLGARTEPVRRITKAKNVNMKYSAAVNVDEVPVTVDWRQKGAVNAIKDQGTCGSCWAFSTAAAVEGINKIVTGELVSLSEQELVDCDKSYNQGCNGGLMDY 170          
The following BLAST results are available for this feature:
BLAST of CN189031 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 156
Match NameE-valueIdentityDescription
RD21A_ARATH3.811e-7767.30Cysteine proteinase RD21a OS=Arabidopsis thaliana ... [more]
ORYA_ORYSJ3.132e-6359.13Oryzain alpha chain OS=Oryza sativa subsp. japonic... [more]
ACTN_ACTDE2.481e-6059.90Actinidain OS=Actinidia deliciosa PE=1 SV=1[more]
ACTN_ACTCH2.101e-5959.42Actinidain OS=Actinidia chinensis PE=1 SV=4[more]
GCP1_ARATH4.680e-5955.02Germination-specific cysteine protease 1 OS=Arabid... [more]
CPR1_ARATH2.403e-5553.47Probable cysteine proteinase At3g19400 OS=Arabidop... [more]
XCP1_ARATH6.993e-5551.64Xylem cysteine proteinase 1 OS=Arabidopsis thalian... [more]
XCP2_ARATH2.250e-5354.74Xylem cysteine proteinase 2 OS=Arabidopsis thalian... [more]
CPR4_ARATH9.451e-5250.47Probable cysteine proteinase At4g11320 OS=Arabidop... [more]
CYSP4_BRANA4.691e-5159.41Cysteine proteinase COT44 (Fragment) OS=Brassica n... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS06_0001D08_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0001D08, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN189031 ID=CN189031; Name=CN189031; organism=Citrus sinensis; type=EST; length=688bp
GCGTTTTAAATCCATCTTCTTCTCCCAAAAAGAAAAAAAAACCCTTCTTT
TCTCAAAAGATGGGTTTGTTTAGATCACCAACCATGGCCATGTTTTTACT
TTTTCTCTTCACTGTTTCATCTGCCTTGGACATGTCGATTGTATCTTATG
ATAAAACCCATGAGTCAAAATCTAGCAGCTGGAGGACCGACGATGAGGTT
ATGGCTATGTTCGAGGCCTGGCTCGTGAAGCATGGCAAGGCTTATAACGC
TTTGGGTGAAAAAGAGAAGAGGTTTGAGATCTTTAAGGAAAATCTTAGGT
TTATTGATGAACATAACTCAGAGAACCGGACCTACAAGGTTGGGTTGAAC
CGGTTTGCTGATTTGACCAACGAGGAGTACCGGTCCATGCATCTGGGTGC
ACGCTTTGGTGCTAAAAGGACTTCCTTGAAATCAAAGAGGAGCAACCGTT
ATCTGCCACGTGTCGGCGAGGCGTTGCCGGACTCTGTTGATTGGAGGAAG
AAAGGAGCTGTCGCTGAAGTTAAAGATCAAGGAAGCTGTGGGAGTTGCTG
GGCATTTTCAACCATTGCAGCTGTAGAGGGTATAAACAAGATTGTTACAG
GTAGCCTGATCTCACTATCCGAGCAGGAGTTGGTGGATTGTGATACATCA
TATAATGAAGGATGTAATGGTGGTTTGATGGACTATGC
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