CK937528

Overview
NameCK937528
Unique NameCK937528
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
Developing fruit albedo at 80 DAFB in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC10_ARATH (Ubiquitin-conjugating enzyme E2 10 OS=Arabidopsis thaliana GN=UBC10 PE=1 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 6.093e-79
Identity = 80/84 (95.24%), Postives = 81/84 (96.43%), Query Frame = 2
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG 634
            PK  FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK+DK KYESTARSWTQKYAMG
Sbjct:   65 PKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDKNKYESTARSWTQKYAMG 148          

HSP 2 Score: 145.976 bits (367), Expect = 6.093e-79
Identity = 67/68 (98.53%), Postives = 68/68 (100.00%), Query Frame = 3
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPS+SPYAGGVFLVTIHFPPDYPFKPPKVA
Sbjct:    1 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVFLVTIHFPPDYPFKPPKVA 68          
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC28_ARATH (Ubiquitin carrier protein E2 28 OS=Arabidopsis thaliana GN=UBC28 PE=2 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 6.093e-79
Identity = 78/84 (92.86%), Postives = 81/84 (96.43%), Query Frame = 2
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG 634
            PK  FRTKVFHPN+NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK+D+ KYESTARSWTQKYAMG
Sbjct:   65 PKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARSWTQKYAMG 148          

HSP 2 Score: 146.362 bits (368), Expect = 6.093e-79
Identity = 67/68 (98.53%), Postives = 68/68 (100.00%), Query Frame = 3
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPY+GGVFLVTIHFPPDYPFKPPKVA
Sbjct:    1 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYPFKPPKVA 68          
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC9_ARATH (SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana GN=UBC9 PE=1 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 1.037e-78
Identity = 79/84 (94.05%), Postives = 81/84 (96.43%), Query Frame = 2
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG 634
            PK  FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK+DK KYESTAR+WTQKYAMG
Sbjct:   65 PKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDKNKYESTARTWTQKYAMG 148          

HSP 2 Score: 146.362 bits (368), Expect = 1.037e-78
Identity = 67/68 (98.53%), Postives = 68/68 (100.00%), Query Frame = 3
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPY+GGVFLVTIHFPPDYPFKPPKVA
Sbjct:    1 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYPFKPPKVA 68          
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC4_SOLLC (Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 3.000e-78
Identity = 78/84 (92.86%), Postives = 81/84 (96.43%), Query Frame = 2
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG 634
            PK  FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK+D+ KYE+TARSWTQKYAMG
Sbjct:   65 PKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYETTARSWTQKYAMG 148          

HSP 2 Score: 144.821 bits (364), Expect = 3.000e-78
Identity = 66/68 (97.06%), Postives = 68/68 (100.00%), Query Frame = 3
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP+DSPYAGGVFLV+IHFPPDYPFKPPKVA
Sbjct:    1 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYAGGVFLVSIHFPPDYPFKPPKVA 68          
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC8_ARATH (Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana GN=UBC8 PE=1 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.477e-77
Identity = 77/84 (91.67%), Postives = 81/84 (96.43%), Query Frame = 2
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG 634
            PK  FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK+D+ KYE+TAR+WTQKYAMG
Sbjct:   65 PKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYEATARNWTQKYAMG 148          

HSP 2 Score: 143.665 bits (361), Expect = 1.477e-77
Identity = 65/68 (95.59%), Postives = 68/68 (100.00%), Query Frame = 3
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP++SPY+GGVFLVTIHFPPDYPFKPPKVA
Sbjct:    1 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYPFKPPKVA 68          
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC11_ARATH (Ubiquitin-conjugating enzyme E2 11 OS=Arabidopsis thaliana GN=UBC11 PE=2 SV=2)

HSP 1 Score: 164.851 bits (416), Expect = 1.036e-75
Identity = 77/84 (91.67%), Postives = 81/84 (96.43%), Query Frame = 2
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG 634
            PK  F+TKV+HPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYK+D+ KYESTARSWTQKYAMG
Sbjct:   65 PKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRSKYESTARSWTQKYAMG 148          

HSP 2 Score: 138.658 bits (348), Expect = 1.036e-75
Identity = 62/68 (91.18%), Postives = 67/68 (98.53%), Query Frame = 3
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MASKRILKELKDLQKDPP++CSAGPVAEDMFHWQATIMGP +SPYAGGVFLV+IHFPPDYPFKPPKV+
Sbjct:    1 MASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYPFKPPKVS 68          
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC30_ARATH (Ubiquitin carrier protein E2 30 OS=Arabidopsis thaliana GN=UBC30 PE=2 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 2.101e-73
Identity = 76/84 (90.48%), Postives = 82/84 (97.62%), Query Frame = 2
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG 634
            PK  FRTKV+HPNINSNGSICLDILKEQWSPALT+SKVLLSICSLLTDPNPDDPLVPEIAH+YK+D+VKYESTA+SWTQKYAMG
Sbjct:   65 PKVAFRTKVYHPNINSNGSICLDILKEQWSPALTVSKVLLSICSLLTDPNPDDPLVPEIAHIYKTDRVKYESTAQSWTQKYAMG 148          

HSP 2 Score: 130.568 bits (327), Expect = 2.101e-73
Identity = 58/68 (85.29%), Postives = 63/68 (92.65%), Query Frame = 3
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MASKRI KEL+DLQ+DPP SCSAGP  +DMF WQATIMGP+DSP+AGGVFLVTIHFPPDYPFKPPKVA
Sbjct:    1 MASKRINKELRDLQRDPPVSCSAGPTGDDMFQWQATIMGPADSPFAGGVFLVTIHFPPDYPFKPPKVA 68          
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC29_ARATH (Ubiquitin carrier protein E2 29 OS=Arabidopsis thaliana GN=UBC29 PE=2 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 3.264e-71
Identity = 73/83 (87.95%), Postives = 79/83 (95.18%), Query Frame = 2
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  FRTKVFHPNINSNG+ICLDILK+QWSPALTISKVLLSICSLLTDPNPDDPLVPEIAH+YK+DK KYE+ ARSWTQKYA+
Sbjct:   65 PKVVFRTKVFHPNINSNGNICLDILKDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHIYKTDKTKYEAMARSWTQKYAL 147          

HSP 2 Score: 130.183 bits (326), Expect = 3.264e-71
Identity = 56/67 (83.58%), Postives = 63/67 (94.03%), Query Frame = 3
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            MA++RILKELK+LQ+DPP SCSAGP  EDMFHWQATIMGP++SPY+GGVFLV IHFPPDYPFKPPKV
Sbjct:    1 MATRRILKELKELQRDPPVSCSAGPTGEDMFHWQATIMGPNESPYSGGVFLVNIHFPPDYPFKPPKV 67          
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC1_MAGGR (Ubiquitin-conjugating enzyme E2-16 kDa OS=Magnaporthe grisea GN=UBC1 PE=3 SV=2)

HSP 1 Score: 145.976 bits (367), Expect = 2.479e-65
Identity = 65/83 (78.31%), Postives = 77/83 (92.77%), Query Frame = 2
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F T+++HPNINSNGSICLDIL++QWSPALTISKVLLSICS+LTDPNPDDPLVPEIAH+YK+ + +YESTAR WT+KYA+
Sbjct:   65 PKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSMLTDPNPDDPLVPEIAHVYKTARAQYESTAREWTRKYAI 147          

HSP 2 Score: 122.865 bits (307), Expect = 2.479e-65
Identity = 55/67 (82.09%), Postives = 59/67 (88.06%), Query Frame = 3
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            MA KRI KEL DL +DPP+SCSAGPV ED+FHWQATIMGPSDSPYAGGVF + IHFP DYPFKPPKV
Sbjct:    1 MALKRINKELTDLGRDPPSSCSAGPVGEDLFHWQATIMGPSDSPYAGGVFFLAIHFPTDYPFKPPKV 67          
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2D4_HUMAN (Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens GN=UBE2D4 PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.479e-65
Identity = 66/83 (79.52%), Postives = 72/83 (86.75%), Query Frame = 2
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            PK  F TK++HPNINSNGSICLDIL+ QWSPALT+SKVLLSICSLL DPNPDDPLVPEIAH YK+D+ KY   AR WTQKYAM
Sbjct:   65 PKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWTQKYAM 147          

HSP 2 Score: 123.25 bits (308), Expect = 2.479e-65
Identity = 54/68 (79.41%), Postives = 59/68 (86.76%), Query Frame = 3
Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            MA KRI KEL DLQ+DPP  CSAGPV +D+FHWQATIMGP+DSPY GGVF +TIHFP DYPFKPPKVA
Sbjct:    1 MALKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVA 68          
The following BLAST results are available for this feature:
BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246
Match NameE-valueIdentityDescription
UBC10_ARATH6.093e-7995.24Ubiquitin-conjugating enzyme E2 10 OS=Arabidopsis ... [more]
UBC28_ARATH6.093e-7992.86Ubiquitin carrier protein E2 28 OS=Arabidopsis tha... [more]
UBC9_ARATH1.037e-7894.05SUMO-conjugating enzyme UBC9 OS=Arabidopsis thalia... [more]
UBC4_SOLLC3.000e-7892.86Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum ... [more]
UBC8_ARATH1.477e-7791.67Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis t... [more]
UBC11_ARATH1.036e-7591.67Ubiquitin-conjugating enzyme E2 11 OS=Arabidopsis ... [more]
UBC30_ARATH2.101e-7390.48Ubiquitin carrier protein E2 30 OS=Arabidopsis tha... [more]
UBC29_ARATH3.264e-7187.95Ubiquitin carrier protein E2 29 OS=Arabidopsis tha... [more]
UBC1_MAGGR2.479e-6578.31Ubiquitin-conjugating enzyme E2-16 kDa OS=Magnapor... [more]
UB2D4_HUMAN2.479e-6579.52Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT
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