CK937496

Overview
NameCK937496
Unique NameCK937496
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length609
Libraries
Library NameType
Developing fruit albedo at 80 DAFB in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK937496 vs. ExPASy Swiss-Prot
Match: H2AX_CICAR (Histone H2AX OS=Cicer arietinum GN=HIS2A PE=2 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 1.101e-50
Identity = 104/126 (82.54%), Postives = 106/126 (84.13%), Query Frame = 1
Query:  157 PKASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPXXXXXXXXXXXXXXXXXXGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKMGKGKGDIGSASQEF 534
            PKASKSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAP                  GNAARDNK NRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKK+GKGKG+IGSASQEF
Sbjct:   14 PKASKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKNNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKVGKGKGEIGSASQEF 139          
BLAST of CK937496 vs. ExPASy Swiss-Prot
Match: H2AXB_ARATH (Probable histone H2AXb OS=Arabidopsis thaliana GN=At1g54690 PE=1 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 2.076e-49
Identity = 102/126 (80.95%), Postives = 104/126 (82.54%), Query Frame = 1
Query:  157 PKASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPXXXXXXXXXXXXXXXXXXGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKMGKGKGDIGSASQEF 534
            PKA+KSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAP                  GNAARDNKK RIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLP K+GK KGDIGSASQEF
Sbjct:   17 PKATKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPSKVGKNKGDIGSASQEF 142          
BLAST of CK937496 vs. ExPASy Swiss-Prot
Match: H2AXA_ARATH (Probable histone H2AXa OS=Arabidopsis thaliana GN=At1g08880 PE=1 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 4.626e-49
Identity = 101/126 (80.16%), Postives = 104/126 (82.54%), Query Frame = 1
Query:  157 PKASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPXXXXXXXXXXXXXXXXXXGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKMGKGKGDIGSASQEF 534
            PKA+KSVSRS KAGLQFPVGRIARFLK+GKYAERVGAGAP                  GNAARDNKK RIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLP K+GK KGDIGSASQEF
Sbjct:   17 PKATKSVSRSSKAGLQFPVGRIARFLKSGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPSKVGKNKGDIGSASQEF 142          
BLAST of CK937496 vs. ExPASy Swiss-Prot
Match: H2AX_PICAB (Histone H2AX OS=Picea abies PE=2 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 6.679e-48
Identity = 99/125 (79.20%), Postives = 103/125 (82.40%), Query Frame = 1
Query:  160 KASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPXXXXXXXXXXXXXXXXXXGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKMGKGKGDIGSASQEF 534
            K++KSVSRS KAGLQFPVGRIARFLKAGKYAERVGAGAP                  GNAARDNKKNRIVPRHIQLAVRNDEELSKLLG+VTIANGGVLPNIHQ LLPKK GK KG+IGSASQEF
Sbjct:   14 KSTKSVSRSQKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGAVTIANGGVLPNIHQVLLPKKSGKDKGEIGSASQEF 138          
BLAST of CK937496 vs. ExPASy Swiss-Prot
Match: H2AXB_ORYSJ (Probable histone H2AXb OS=Oryza sativa subsp. japonica GN=Os12g0530000 PE=2 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 1.645e-46
Identity = 98/125 (78.40%), Postives = 101/125 (80.80%), Query Frame = 1
Query:  160 KASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPXXXXXXXXXXXXXXXXXXGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKMGKGKGDIGSASQEF 534
            K SKSVSRS KAGLQFPVGRIAR+LKAGKYAERVGAGAP                  GNAARDNKKNRIVPRHIQLAVRNDEELS+LLG+VTIA GGVLPNIHQTLLPKK GK K DIGSASQEF
Sbjct:   14 KGSKSVSRSSKAGLQFPVGRIARYLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSRLLGAVTIAAGGVLPNIHQTLLPKKGGKDKADIGSASQEF 138          
BLAST of CK937496 vs. ExPASy Swiss-Prot
Match: H2AXB_ORYSI (Probable histone H2AXb OS=Oryza sativa subsp. indica GN=OsI_037312 PE=3 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 1.645e-46
Identity = 98/125 (78.40%), Postives = 101/125 (80.80%), Query Frame = 1
Query:  160 KASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPXXXXXXXXXXXXXXXXXXGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKMGKGKGDIGSASQEF 534
            K SKSVSRS KAGLQFPVGRIAR+LKAGKYAERVGAGAP                  GNAARDNKKNRIVPRHIQLAVRNDEELS+LLG+VTIA GGVLPNIHQTLLPKK GK K DIGSASQEF
Sbjct:   14 KGSKSVSRSSKAGLQFPVGRIARYLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSRLLGAVTIAAGGVLPNIHQTLLPKKGGKDKADIGSASQEF 138          
BLAST of CK937496 vs. ExPASy Swiss-Prot
Match: H2AXA_ORYSJ (Probable histone H2AXa OS=Oryza sativa subsp. japonica GN=Os03g0721900 PE=3 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 5.851e-44
Identity = 95/125 (76.00%), Postives = 100/125 (80.00%), Query Frame = 1
Query:  160 KASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPXXXXXXXXXXXXXXXXXXGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKMGKGKGDIGSASQEF 534
            K +K+VSRS KAGLQFPVGRIAR+LKAGKYAERVGAGAP                  GNAARDNKKNRIVPRHIQLAVRNDEELS+LLG+VTIA GGVLPNI Q LLPKK G GKGDIGSASQEF
Sbjct:   14 KTTKAVSRSSKAGLQFPVGRIARYLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSRLLGTVTIAAGGVLPNIQQVLLPKK-GGGKGDIGSASQEF 137          
BLAST of CK937496 vs. ExPASy Swiss-Prot
Match: H2AXA_ORYSI (Probable histone H2AXa OS=Oryza sativa subsp. indica GN=OsI_012923 PE=3 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 5.851e-44
Identity = 95/125 (76.00%), Postives = 100/125 (80.00%), Query Frame = 1
Query:  160 KASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPXXXXXXXXXXXXXXXXXXGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKMGKGKGDIGSASQEF 534
            K +K+VSRS KAGLQFPVGRIAR+LKAGKYAERVGAGAP                  GNAARDNKKNRIVPRHIQLAVRNDEELS+LLG+VTIA GGVLPNI Q LLPKK G GKGDIGSASQEF
Sbjct:   14 KTTKAVSRSSKAGLQFPVGRIARYLKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSRLLGTVTIAAGGVLPNIQQVLLPKK-GGGKGDIGSASQEF 137          
BLAST of CK937496 vs. ExPASy Swiss-Prot
Match: H2A3_ARATH (Probable histone H2A.3 OS=Arabidopsis thaliana GN=At4g27230 PE=1 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 1.948e-39
Identity = 85/114 (74.56%), Postives = 86/114 (75.44%), Query Frame = 1
Query:  163 ASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPXXXXXXXXXXXXXXXXXXGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKMGKGK 504
            A KS SRS KAGLQFPVGRIARFLKAGKYAERVGAGAP                  GNAARDNKK RIVPRHIQLAVRNDEELSKLLG VTIANGGV+PNIH  LLPKK G  K
Sbjct:   13 AKKSTSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIANGGVMPNIHNLLLPKKAGSSK 126          
BLAST of CK937496 vs. ExPASy Swiss-Prot
Match: H2A6_ARATH (Histone H2A.6 OS=Arabidopsis thaliana GN=RAT5 PE=1 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 5.668e-39
Identity = 84/114 (73.68%), Postives = 86/114 (75.44%), Query Frame = 1
Query:  163 ASKSVSRSHKAGLQFPVGRIARFLKAGKYAERVGAGAPXXXXXXXXXXXXXXXXXXGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGSVTIANGGVLPNIHQTLLPKKMGKGK 504
            A K+ SRS KAGLQFPVGRIARFLKAGKYAERVGAGAP                  GNAARDNKK RIVPRHIQLAVRNDEELSKLLG VTIANGGV+PNIH  LLPKK G  K
Sbjct:   13 AKKATSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIANGGVMPNIHNLLLPKKAGASK 126          
The following BLAST results are available for this feature:
BLAST of CK937496 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 239
Match NameE-valueIdentityDescription
H2AX_CICAR1.101e-5082.54Histone H2AX OS=Cicer arietinum GN=HIS2A PE=2 SV=1[more]
H2AXB_ARATH2.076e-4980.95Probable histone H2AXb OS=Arabidopsis thaliana GN=... [more]
H2AXA_ARATH4.626e-4980.16Probable histone H2AXa OS=Arabidopsis thaliana GN=... [more]
H2AX_PICAB6.679e-4879.20Histone H2AX OS=Picea abies PE=2 SV=1[more]
H2AXB_ORYSJ1.645e-4678.40Probable histone H2AXb OS=Oryza sativa subsp. japo... [more]
H2AXB_ORYSI1.645e-4678.40Probable histone H2AXb OS=Oryza sativa subsp. indi... [more]
H2AXA_ORYSJ5.851e-4476.00Probable histone H2AXa OS=Oryza sativa subsp. japo... [more]
H2AXA_ORYSI5.851e-4476.00Probable histone H2AXa OS=Oryza sativa subsp. indi... [more]
H2A3_ARATH1.948e-3974.56Probable histone H2A.3 OS=Arabidopsis thaliana GN=... [more]
H2A6_ARATH5.668e-3973.68Histone H2A.6 OS=Arabidopsis thaliana GN=RAT5 PE=1... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004780_A06 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IVF_A06 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937496 ID=CK937496; Name=CK937496; organism=Citrus sinensis; type=EST; length=609bp
GCGGGGATTCNNNAAAAATACTCAAAAGCAATNNNACCGATCTTTTTTGT
GGCTTCACTTTCAATCGCTTCAAACCAAACCGAAATCTAGGGTTAGGGTT
TTTCTGTAATAAGAATGAGTTCAACTGGAGGGTCAACGAAGGGCGGCAGG
GGTAAGCCGAAGGCTTCGAAATCGGTCTCGCGGTCGCATAAGGCGGGCCT
TCAGTTCCCGGTGGGCAGGATCGCTAGGTTTTTGAAGGCCGGAAAGTACG
CTGAGCGAGTCGGCGCCGGAGCTCCTGTTTACCTCTCTGCCGTGCTTGAG
TACCTCGCTGCTGAGGTTCTGGAGCTTGCTGGCAATGCAGCAAGGGACAA
CAAGAAGAACCGTATAGTGCCAAGGCATATTCAGCTTGCAGTTCGAAATG
ATGAGGAATTGAGCAAGCTGCTGGGCTCGGTTACCATTGCCAATGGAGGT
GTTTTACCCAACATTCACCAGACGCTGCTTCCCAAGAAGATGGGGAAAGG
AAAGGGTGACATTGGTTCTGCTTCTCAAGAGTTCTAGACTCTGCTCTGTC
AGAATTTTACTAGTATCTGTAAAAAAGGACAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA
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