CK936881

Overview
NameCK936881
Unique NameCK936881
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length794
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK936881 vs. ExPASy Swiss-Prot
Match: C93B1_GLYEC (Licodione synthase OS=Glycyrrhiza echinata GN=CYP93B1 PE=1 SV=2)

HSP 1 Score: 224.942 bits (572), Expect = 3.897e-58
Identity = 113/235 (48.09%), Postives = 158/235 (67.23%), Query Frame = 2
Query:   92 KHSRRLPPSPW-ALPIVXXXXXXXXXXXXXFHKLSTRYGPLMSVRIGSVLGVVTSCPDVTKELLKTNDVTFTGRKSSAAIECLTYNSSFAFAPYGPYWQFMKKLSAVELLGSRTLHQFLPVRTNELRELIRFLFEKSKSGQSVNITDELLRFANNIISQMMLSIRCSGKGGQAEECRTLAREVTEIFGEFNISDIIWIFKSFDIQGFNRRFKDIHXXYDSQLENIITNREKLRKE 793
            ++ + LPPSP+  LPI+             FH LS RYGPL S+  GSVL VV S P   K+LL+TN++ F  R  S A++ LTY SS AFAPYG YW+F+KKLS  ELLGSR+++ F  +R  E  +L+R L  ++++ ++VNIT+ELL+  NN+IS MM+        G+AEE R + R+VTEIFGEFN+SD IW+FK  D+QGF +R +D+   +D+ +E II+ RE+ RK+
Sbjct:   27 RYGKNLPPSPFFRLPIIGHMHMLGPLLHQSFHNLSHRYGPLFSLNFGSVLCVVASTPHFAKQLLQTNELAFNCRIESTAVKKLTYESSLAFAPYGDYWRFIKKLSMNELLGSRSINNFQHLRAQETHQLLRLLSNRARAFEAVNITEELLKLTNNVISIMMV--------GEAEEARDVVRDVTEIFGEFNVSDFIWLFKKMDLQGFGKRIEDLFQRFDTLVERIISKREQTRKD 253          
BLAST of CK936881 vs. ExPASy Swiss-Prot
Match: C93A1_SOYBN (Cytochrome P450 93A1 OS=Glycine max GN=CYP93A1 PE=2 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 2.213e-53
Identity = 110/261 (42.15%), Postives = 159/261 (60.92%), Query Frame = 2
Query:   23 MALQPLIFYASLFILSALVLKAIKHSRRLPPSPWALPIVXXXXXXXXXXXXXFHKLSTRYGPLMSVRIGSVLGVVTSCPDVTKELLKTNDVTFTGRKS-SAAIECLTYNSS---FAFAPYGPYWQFMKKLSAVELLGSRTLHQFLPVRTNELRELIRFLFEKSKSGQSVNITDELLRFANNIISQMMLSIRCSGKGGQAEECRTLAREVTEIFGEFNISDIIWIFKSFDIQGFNRRFKDIHXXYDSQLENIITNREKLRKE 793
            MA Q L+      I+ A +L   +  + LPPSP ALPI+             F+KLSTR+GP+M + +GSV  VV S  +  KE LKT+++ F+ R   + A++ L Y+S    FAFAP+GPYW+FMKKL   ELL  R + QFLPVR  E +  I  +F K  +G++V+  DEL+  +NNI+S+M LS + S    QAEE + L   + E+ G+FN+SD IW  K FD+QGFNR+ K+    +D  ++ II  R++ R++
Sbjct:    1 MAYQVLLICLVSTIVFAYILWRKQSKKNLPPSPKALPIIGHLHLVSPIPHQDFYKLSTRHGPIMQLFLGSVPCVVASTAEAAKEFLKTHEINFSNRPGQNVAVKGLAYDSQDFLFAFAPFGPYWKFMKKLCMSELLSGRMMDQFLPVRQQETKRFISRVFRKGVAGEAVDFGDELMTLSNNIVSRMTLSQKTSENDNQAEEMKKLVSNIAELMGKFNVSDFIWYLKPFDLQGFNRKIKETRDRFDVVVDGIIKQRQEERRK 261          
BLAST of CK936881 vs. ExPASy Swiss-Prot
Match: C93A3_SOYBN (Cytochrome P450 93A3 OS=Glycine max GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 1.641e-48
Identity = 109/261 (41.76%), Postives = 155/261 (59.39%), Query Frame = 2
Query:   23 MALQPLIFYASLFILSALVL--KAIKHSRRLPPSPWALPIVXXXXXXXXXXXXXFHKLSTRYGPLMSVRIGSVLGVVTSCPDVTKELLKTNDVTFTGRKSSA-AIECLTYN-SSFAFAPYGPYWQFMKKLSAVELLGSRTLHQFLPVRTNELRELIRFLFEKSKSGQSVNITDELLRFANNIISQMMLSIRCSGKG-GQAEECRTLAREVTEIFGEFNISDIIWIFKSFDIQGFNRRFKDIHXXYDSQLENIITNREKLRK 790
            MA Q L       I+ A +L  K  K+   LPPSP  LPI+             FHKLS RYGP++ + +GSV  VV S  +  KE LKT++  F+ R ++  A+E LTY    F FAPYGPYW+FMKKL   ELLG   L QFLPVR  E ++ I+ + +K  SG++V+   E +  +NNI+S+M++S   + +   + EE R L ++  E+ G+FNISD +   K FD+QGFN+R + I   +D+ L+ II  RE+ R+
Sbjct:    1 MAFQVLFICLISTIVFASILWRKQNKNKTLLPPSPMPLPIIGHLHLLSPTPHQDFHKLSLRYGPIIHLFLGSVPCVVASTAEAAKEFLKTHEPAFSNRPANTVAVETLTYGFQDFLFAPYGPYWKFMKKLCMSELLGGHMLDQFLPVRQXETKKFIKRVLQKGISGEAVDFGGEFITLSNNIVSRMIVSQTSTTEDENEVEEMRKLVKDAAELSGKFNISDFVSFLKRFDLQGFNKRLEKIRDCFDTVLDRIIKQREEERR 261          
BLAST of CK936881 vs. ExPASy Swiss-Prot
Match: C93A2_SOYBN (Cytochrome P450 93A2 OS=Glycine max GN=CYP93A2 PE=2 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 4.775e-48
Identity = 104/257 (40.47%), Postives = 152/257 (59.14%), Query Frame = 2
Query:   23 MALQPLIFYASLFILSALVLKAIKHSRRLPPSPWALPIVXXXXXXXXXXXXXFHKLSTRYGPLMSVRIGSVLGVVTSCPDVTKELLKTNDVTFTGRKS-SAAIECLTYNSSFAFAPYGPYWQFMKKLSAVELLGSRTLHQFLPVRTNELRELIRFLFEKSKSGQSVNITDELLRFANNIISQMMLSIRCSGKGGQAEECRTLAREVTEIFGEFNISDIIWIFKSFDIQGFNRRFKDIHXXYDSQLENIITNREKLRK 790
            MA Q L+      I+ A ++   +  ++LPPSP  LPI+             F+KLS R+GP+M + +GSV  VV S  +  KE LKT+++ F+ R   + A++ LTY     F PYGP  +F+KKL   ELLG R L QFLPVR  E ++ I+ + +K  +G++V+   E +R +NNIIS+M ++   S    QAEE R L  +V E+ G FN+SD IW  K FD+QGFN+R +     +D+ L+ II  RE+ R+
Sbjct:    1 MAYQVLVICVVSSIVFAYIVWRKERKKKLPPSPKGLPIIGHLHLVSPIPHQDFYKLSLRHGPIMQLFLGSVPCVVASTAEAAKEFLKTHEINFSNRPGQNVAVQFLTY----VFGPYGPSVKFIKKLCMSELLGGRMLDQFLPVRQQETKKFIKRVLQKGIAGEAVDFGGEFMRLSNNIISRMTMNQTSSEDEKQAEEMRMLVADVAELMGTFNVSDFIWFLKPFDLQGFNKRIRKTRIRFDAVLDRIIKQREEERR 253          
BLAST of CK936881 vs. ExPASy Swiss-Prot
Match: C75A2_SOLME (Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.544e-33
Identity = 79/254 (31.10%), Postives = 126/254 (49.61%), Query Frame = 2
Query:   38 LIFYASLFILSALVLKAIKHSRRLPPSPWALPIVXXXXXXXXXXXXXFHKLSTRYGPLMSVRIGSVLGVVTSCPDVTKELLKTNDVTFTGRKSSAAIECLTYNSS-FAFAPYGPYWQFMKKLSAVELLGSRTLHQFLPVRTNELRELIRFLFEKSKSGQSVNITDELLRFANNIISQMMLSIRC-SGKGGQAEECRTLAREVTEIFGEFNISDIIWIFKSFDIQGFNRRFKDIHXXYDSQLENIITNREKLRKE 793
            +I+     I+  L+       RRLPP P   P++               K++ +YGP+M +++G+   VV S P+  K  LKT D+ F+ R  +A    + YN+    FAPYGP W+ ++KLS + +LG + L  +  VR NEL  +++ +F+ S  G+ + + D L     N+I Q+MLS R    KG +  E + +  E+  + G FNI D I      D+QG  +  K +H  +D  L  +    E    E
Sbjct:   13 IIYIIVYIIIQKLIATGSWRRRRLPPGPEGWPVIGALPLLGGMPHVALAKMAKKYGPIMYLKVGTCGMVVASTPNAAKAFLKTLDINFSNRPPNAGATHMAYNAQDMVFAPYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDASHVGERIVVADMLTFAMANMIGQVMLSKRVFVEKGKEVNEFKNMVVELMTVAGYFNIGDFIPQIAWMDLQGIEKGMKKLHKKFDDLLTKMFEEHEATSNE 266          
BLAST of CK936881 vs. ExPASy Swiss-Prot
Match: C75A1_PETHY (Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida GN=CYP75A1 PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.396e-31
Identity = 80/240 (33.33%), Postives = 123/240 (51.25%), Query Frame = 2
Query:   41 IFYASLFILSALVLKAIKHSRRLPPSPWALPIVXXXXXXXXXXXXXFHKLSTRYGPLMSVRIGSVLGVVTSCPDVTKELLKTNDVTFTGRKSSAAIECLTYNSS-FAFAPYGPYWQFMKKLSAVELLGSRTLHQFLPVRTNELRELIRFLFEKSKSGQSVNITDELLRFANNIISQMMLSIRC-SGKGGQAEECRTLAREVTEIFGEFNISDIIWIFKSFDIQGFNRRFKDIHXXYDSQL 754
            IF  +  I+S L+ K     R LPP P   P++               K++ +YG +M +++G+    V S PD  K  LKT D+ F+ R  +A    L YN+    FA YGP W+ ++KLS + +LG + L  +  VR NEL  +++ + + S+ GQ V + + L     N+I Q+MLS R    KG +  E + +  E+  I G FNI D I      D+QG  +R K +H  +D+ L
Sbjct:   13 IFLIAHIIISTLISKTT--GRHLPPGPRGWPVIGALPLLGAMPHVSLAKMAKKYGAIMYLKVGTCGMAVASTPDAAKAFLKTLDINFSNRPPNAGATHLAYNAQDMVFAHYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMSDMSREGQRVVVAEMLTFAMANMIGQVMLSKRVFVDKGVEVNEFKDMVVELMTIAGYFNIGDFIPCLAWMDLQGIEKRMKRLHKKFDALL 250          
BLAST of CK936881 vs. ExPASy Swiss-Prot
Match: C75A3_PETHY (Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida GN=CYP75A3 PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 4.061e-31
Identity = 79/246 (32.11%), Postives = 127/246 (51.63%), Query Frame = 2
Query:   23 MALQPLIFYASLFILSALVLKAIKHSRRLPPSPWALPIVXXXXXXXXXXXXXFHKLSTRYGPLMSVRIGSVLGVVTSCPDVTKELLKTNDVTFTGRKSSAAIECLTYNSS-FAFAPYGPYWQFMKKLSAVELLGSRTLHQFLPVRTNELRELIRFLFEKSKSGQSVNITDELLRFANNIISQMMLSIRC-SGKGGQAEECRTLAREVTEIFGEFNISDIIWIFKSFDIQGFNRRFKDIHXXYDSQL 754
            +A   LIF  +   +S L+  +I + RRLPP P   P++               K++ +YG +M +++G+   VV S PD  K  LKT D+ F+ R  +A    L Y +    FA YGP W+ ++KLS + +LG + L  +  VR NEL  +++ +F+ S+ G+ V + + L     N+I Q++LS R    KG +  E + +  E+    G FNI D I      D+QG  +  K +H  +D+ L
Sbjct:    7 LAAATLIFLTTHIFISTLL--SITNGRRLPPGPRGWPVIGALPLLGAMPHVSLAKMAKKYGAIMYLKVGTCGMVVASTPDAAKAFLKTLDLNFSNRPPNAGATHLAYGAQDMVFAHYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDMSREGERVVVAEMLTFAMANMIGQVILSKRVFVNKGVEVNEFKDMVVELMTTAGYFNIGDFIPCLAWMDLQGIEKGMKRLHKKFDALL 250          
BLAST of CK936881 vs. ExPASy Swiss-Prot
Match: C71AR_ARATH (Cytochrome P450 71A27 OS=Arabidopsis thaliana GN=CYP71A27 PE=2 SV=3)

HSP 1 Score: 131.724 bits (330), Expect = 4.490e-30
Identity = 80/239 (33.47%), Postives = 123/239 (51.46%), Query Frame = 2
Query:   59 FILSALVLKAIKHSR-RLPPSPWALPIVXXXXXXXXXXXXXFHKLSTRYGPLMSVRIGSVLGVVTSCPDVTKELLKTNDVTFTGRKSSAAIEC-LTYNSSFAFAPYGPYWQFMKKLSAVELLGSRTLHQFLPVRTNELRELIRFLFEKSKSGQSVNITDELLRFANNIISQMMLSIRCSGKGGQAEECRTLAREVTEIFGEFNISDIIWIFKSFD-IQGFNRRFKDIHXXYDSQLENII 766
            F+    +LK I  ++ +LPPSPW LP++              H LS RYGPLM +  G V  +V SCPDVT +++KT+D+ F  R  S AI   +       F PYG  W+ MK L  V LL ++ +  F  +R  E++ +   L E S S  SVN++  L+   N+II ++ L  + + + G   + + L    +E FG+F   D I      D I G + + KDI+   D  L++++
Sbjct:   16 FLFLKPLLKRITTTKPKLPPSPWRLPVIGNLHQLGPNPHRYLHSLSLRYGPLMLLHFGRVPVLVVSCPDVTNDIMKTHDLKFANRPKSKAINIFMEGGRDIIFGPYGEDWKSMKSLGVVHLLNNKMVRSFENLREEEIKVMTEKLEEASSSSSSVNLSKLLMTLTNDIICRITLGRKYNEEEG-GIDIKNLVMTSSEFFGKFFFGDFIPSLAWIDWISGIDDKMKDINNKLDCFLDSMV 253          
BLAST of CK936881 vs. ExPASy Swiss-Prot
Match: C75A4_GENTR (Flavonoid 3',5'-hydroxylase OS=Gentiana triflora GN=CYP75A4 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 1.306e-29
Identity = 81/262 (30.92%), Postives = 134/262 (51.15%), Query Frame = 2
Query:   17 LAMALQPLIFYASLFILSALVLKAIKHS-RRLPPSPWALPIVXXXXXXXXXXXXXFHKLSTRYGPLMSVRIGSVLGVVTSCPDVTKELLKTNDVTFTGRKSSAAIECLTYNSS-FAFAPYGPYWQFMKKLSAVELLGSRTLHQFLPVRTNELRELIRFLFEKSKSGQSVNITDELLRFANNIISQMMLSIRC-SGKGGQAEECRTLAREVTEIFGEFNISDIIWIFKSFDIQGFNRRFKDIHXXYDSQLENIITNREKLRKE 793
            L +A    +F+    ++  L  KA  H  RRLPP P   PI+             F  ++ +YG +M +++GS    + S PD  K  LKT D+ F+ R  +A    L YN+    FA YGP W+ ++KLS + +LG + L  +  VR  EL  +++ +FE S++ + V I++ L     N++SQ++LS R  + KG ++ E + +  E+    G FNI D I      D+QG     K +H  +D  L  ++ + ++  +E
Sbjct:   10 LHLATALFLFFHVQKLVHYLHGKATGHRCRRLPPGPTGWPILGALPLLGNMPHVTFANMAKKYGSVMYLKVGSHGLAIASTPDAAKAFLKTLDLNFSNRPPNAGATHLAYNAQDMVFAHYGPKWKLLRKLSNLHMLGGKALENWADVRKTELGYMLKAMFESSQNNEPVMISEMLTYAMANMLSQVILSRRVFNKKGAKSNEFKDMVVELMTSAGYFNIGDFIPSIGWMDLQGIEGGMKRLHKKFDVLLTRLLDDHKRTSQE 271          
BLAST of CK936881 vs. ExPASy Swiss-Prot
Match: C71AC_ARATH (Cytochrome P450 71A12 OS=Arabidopsis thaliana GN=CYP71A12 PE=2 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 8.468e-29
Identity = 76/243 (31.28%), Postives = 123/243 (50.62%), Query Frame = 2
Query:   53 SLFILSALVLKAIKHSRRLPPSPWALPIVXXXXXXXXXXXXXFHKLSTRYGPLMSVRIGSVLGVVTSCPDVTKELLKTNDVTFTGRKSSAAIECLTYNS-SFAFAPYGPYWQFMKKLSAVELLGSRTLHQFLPVRTNELRELIRFLFEKSKSGQSVNITDELLRFANNIISQMMLSIRCSGKGGQAEECRTLAREVTEIFGEFNISDII----WIFKSFDIQGFNRRFKDIHXXYDSQLENII 766
            +LF+L    LK   +   LPPSPW LP++              H LS RYGPLM +  G V  +V S  +  +E+LKT+D+ F  R  S A+  L        F PYG YW+ MK +  + LL ++ +  F  +R  EL E+I+ L + S S  S N+++  +   +++ S++ L  R   +   A + +   R++ E+ GEF I D +    WI +   I GFN R K++   +   ++ ++
Sbjct:   16 TLFLLKQF-LKRTANKVNLPPSPWRLPLIGNLHQLSLHPHRSLHSLSLRYGPLMLLHFGRVPILVVSSGEAAQEVLKTHDLKFANRPRSKAVHGLMNGGRDVVFGPYGEYWRQMKSVCILNLLTNKMVASFEKIREEELNEMIKKLEKASSSSSSENLSELFVTLPSDVTSRIALG-RKHSEDETARDLKKRVRQIMELLGEFPIGDYVPALAWIDR---INGFNARIKEVSQGFSDLMDKVV 253          
The following BLAST results are available for this feature:
BLAST of CK936881 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 113
Match NameE-valueIdentityDescription
C93B1_GLYEC3.897e-5848.09Licodione synthase OS=Glycyrrhiza echinata GN=CYP9... [more]
C93A1_SOYBN2.213e-5342.15Cytochrome P450 93A1 OS=Glycine max GN=CYP93A1 PE=... [more]
C93A3_SOYBN1.641e-4841.76Cytochrome P450 93A3 OS=Glycine max GN=CYP93A3 PE=... [more]
C93A2_SOYBN4.775e-4840.47Cytochrome P450 93A2 OS=Glycine max GN=CYP93A2 PE=... [more]
C75A2_SOLME2.544e-3331.10Flavonoid 3',5'-hydroxylase OS=Solanum melongena G... [more]
C75A1_PETHY1.396e-3133.33Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida G... [more]
C75A3_PETHY4.061e-3132.11Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida G... [more]
C71AR_ARATH4.490e-3033.47Cytochrome P450 71A27 OS=Arabidopsis thaliana GN=C... [more]
C75A4_GENTR1.306e-2930.92Flavonoid 3',5'-hydroxylase OS=Gentiana triflora G... [more]
C71AC_ARATH8.468e-2931.28Cytochrome P450 71A12 OS=Arabidopsis thaliana GN=C... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004516_H06 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IIIF_H06 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936881 ID=CK936881; Name=CK936881; organism=Citrus sinensis; type=EST; length=794bp
GGGCTAGAGATCTTTTCTAGCCATGGCACTTCAACCACTGATTTTTTATG
CTTCCCTCTTCATCTTGTCCGCCCTAGTGCTGAAAGCCATCAAACACTCA
CGGCGACTGCCACCGAGCCCGTGGGCCTTGCCGATCGTCGGGCACCTCCA
TCTCCTCGGCCCTTCCCTTCACCATTCTTTTCACAAGCTTTCAACTCGTT
ATGGCCCCTTGATGTCCGTACGCATAGGCTCTGTCCTAGGTGTTGTAACT
TCTTGTCCTGATGTAACCAAAGAGCTTCTCAAAACAAATGATGTTACTTT
TACCGGCCGGAAAAGCTCTGCCGCCATTGAATGCCTTACATATAACTCTT
CTTTTGCTTTCGCTCCCTATGGCCCATATTGGCAATTCATGAAAAAGCTG
TCCGCAGTTGAGCTCTTGGGCAGCAGAACCCTTCACCAGTTTCTACCTGT
TCGAACAAATGAGTTGCGCGAGCTTATTCGGTTTCTATTTGAGAAGTCTA
AATCTGGCCAAAGTGTTAATATTACAGATGAATTGTTGAGATTTGCAAAC
AATATAATATCTCAAATGATGTTGAGCATTAGATGTTCAGGAAAGGGGGG
CCAGGCTGAAGAGTGTAGAACCCTGGCTCGTGAAGTGACTGAAATTTTTG
GCGAGTTTAATATCTCTGACATTATTTGGATTTTCAAAAGCTTTGATATT
CAGGGTTTTAACAGGAGATTCAAGGATATTCATCNNNNGTACGATTCGCA
GCTGGAGAATATCATAACAAATCGTGAAAAATTAAGGAAGGAGA
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