CX295634

Overview
NameCX295634
Unique NameCX295634
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length712
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295634 vs. ExPASy Swiss-Prot
Match: PHSH_VICFA (Alpha-glucan phosphorylase, H isozyme OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 8.542e-43
Identity = 85/120 (70.83%), Postives = 95/120 (79.17%), Query Frame = 1
Query:  352 EPSAIASNISYHVQYSPHFSPTKFEPEQAFFATAESVRDRLIQQWNETYHHFNKVDPKQTYYLSMEFLQGRTLTNAIGSLDIQNAYADALNNLGHVLEEIAEQEKDXXXXXXXXXXXXSC 711
            +P  IASNISYH QY+PHFSP KF+ +QA++ATAESVRDRLIQQWNETY HF+KVDPKQTYYLSMEFLQGR LTNAIG+L+IQ+AYADAL   G  LEEI EQEKD            SC
Sbjct:   29 KPDEIASNISYHAQYTPHFSPFKFQLQQAYYATAESVRDRLIQQWNETYLHFHKVDPKQTYYLSMEFLQGRALTNAIGNLNIQDAYADALRKFGLELEEITEQEKDAALGNGGLGRLASC 148          
BLAST of CX295634 vs. ExPASy Swiss-Prot
Match: PHSH_ARATH (Alpha-glucan phosphorylase, H isozyme OS=Arabidopsis thaliana GN=At3g46970 PE=2 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 5.537e-42
Identity = 87/144 (60.42%), Postives = 97/144 (67.36%), Query Frame = 1
Query:  280 AKANGKNEXXXXXXXXXXXXXXXXEPSAIASNISYHVQYSPHFSPTKFEPEQAFFATAESVRDRLIQQWNETYHHFNKVDPKQTYYLSMEFLQGRTLTNAIGSLDIQNAYADALNNLGHVLEEIAEQEKDXXXXXXXXXXXXSC 711
            A ANGK                  + + IA NI YH +YSPHFSP KF PEQA +ATAES+RDRLIQ WNETY HFNKVDPKQTYYLSME+LQGR LTNAIG+L++Q  YADAL  LG+ LEEIAEQEKD            SC
Sbjct:    2 ANANGKAATSLPEKISAKANPEADDATEIAGNIVYHAKYSPHFSPLKFGPEQALYATAESLRDRLIQLWNETYVHFNKVDPKQTYYLSMEYLQGRALTNAIGNLNLQGPYADALRTLGYELEEIAEQEKDAALGNGGLGRLASC 145          
BLAST of CX295634 vs. ExPASy Swiss-Prot
Match: PHSH_SOLTU (Alpha-glucan phosphorylase, H isozyme OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 2.104e-41
Identity = 81/121 (66.94%), Postives = 96/121 (79.34%), Query Frame = 1
Query:  352 EPSAIASNISYHVQYSPHFSPTKFEPEQAFFA-TAESVRDRLIQQWNETYHHFNKVDPKQTYYLSMEFLQGRTLTNAIGSLDIQNAYADALNNLGHVLEEIAEQEKDXXXXXXXXXXXXSC 711
            +P+ IASNI YH QY+PHFSP KFEP QA++A TA+SVRDRLI+QWN+TY H++KV+PKQTYYLSME+LQGR LTNA+G+LDI NAYADALN LG  LEE+ EQEKD            SC
Sbjct:   22 DPTDIASNIKYHAQYTPHFSPFKFEPLQAYYAATADSVRDRLIKQWNDTYLHYDKVNPKQTYYLSMEYLQGRALTNAVGNLDIHNAYADALNKLGQQLEEVVEQEKDAALGNGGLGRLASC 142          
BLAST of CX295634 vs. ExPASy Swiss-Prot
Match: PHSH_WHEAT (Alpha-glucan phosphorylase, H isozyme OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 3.968e-40
Identity = 79/120 (65.83%), Postives = 90/120 (75.00%), Query Frame = 1
Query:  352 EPSAIASNISYHVQYSPHFSPTKFEPEQAFFATAESVRDRLIQQWNETYHHFNKVDPKQTYYLSMEFLQGRTLTNAIGSLDIQNAYADALNNLGHVLEEIAEQEKDXXXXXXXXXXXXSC 711
            +PSAIA NISYH QYSPHFSP  F PEQAF+ATAESVRD L+Q+WN+TY HF+K DPKQTYYLSME+LQGR LTNA+G+L I  AYADAL   G+ LE IA QE+D            SC
Sbjct:   17 DPSAIAGNISYHAQYSPHFSPLAFGPEQAFYATAESVRDHLLQRWNDTYLHFHKTDPKQTYYLSMEYLQGRALTNAVGNLAITGAYADALKKFGYELEAIAGQERDAALGNGGLGRLASC 136          
BLAST of CX295634 vs. ExPASy Swiss-Prot
Match: PHSL_IPOBA (Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Ipomoea batatas PE=2 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 2.182e-30
Identity = 63/120 (52.50%), Postives = 83/120 (69.17%), Query Frame = 1
Query:  352 EPSAIASNISYHVQYSPHFSPTKFEPEQAFFATAESVRDRLIQQWNETYHHFNKVDPKQTYYLSMEFLQGRTLTNAIGSLDIQNAYADALNNLGHVLEEIAEQEKDXXXXXXXXXXXXSC 711
            + ++IAS+I YH ++SP FSP +FE  +A+FATA+SVRD LI  WN TY ++ K++ KQ YYLSMEFLQGR L NAIG+L++   YA+ALN LGH LE +A +E D            SC
Sbjct:   68 DAASIASSIKYHAEFSPAFSPERFELPKAYFATAQSVRDALIVNWNATYDYYEKLNMKQAYYLSMEFLQGRALLNAIGNLELTGEYAEALNKLGHNLENVASKEPDAALGNGGLGRLASC 187          
BLAST of CX295634 vs. ExPASy Swiss-Prot
Match: PHSL1_SOLTU (Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic OS=Solanum tuberosum PE=1 SV=2)

HSP 1 Score: 129.413 bits (324), Expect = 1.847e-29
Identity = 62/120 (51.67%), Postives = 82/120 (68.33%), Query Frame = 1
Query:  352 EPSAIASNISYHVQYSPHFSPTKFEPEQAFFATAESVRDRLIQQWNETYHHFNKVDPKQTYYLSMEFLQGRTLTNAIGSLDIQNAYADALNNLGHVLEEIAEQEKDXXXXXXXXXXXXSC 711
            + ++I S+I YH +++P FSP +FE  +AFFATA+SVRD L+  WN TY  + K++ KQ YYLSMEFLQGR L NAIG+L++  A+A+AL NLGH LE +A QE D            SC
Sbjct:   75 DAASITSSIKYHAEFTPVFSPERFELPKAFFATAQSVRDSLLINWNATYDIYEKLNMKQAYYLSMEFLQGRALLNAIGNLELTGAFAEALKNLGHNLENVASQEPDAALGNGGLGRLASC 194          
BLAST of CX295634 vs. ExPASy Swiss-Prot
Match: PHSL2_SOLTU (Alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic OS=Solanum tuberosum GN=STP-1 PE=1 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 5.375e-29
Identity = 61/120 (50.83%), Postives = 80/120 (66.67%), Query Frame = 1
Query:  352 EPSAIASNISYHVQYSPHFSPTKFEPEQAFFATAESVRDRLIQQWNETYHHFNKVDPKQTYYLSMEFLQGRTLTNAIGSLDIQNAYADALNNLGHVLEEIAEQEKDXXXXXXXXXXXXSC 711
            + +++ S+I YH +++P FSP KFE  +A++ATAESVRD LI  WN TY  + K++ KQ YYLSMEFLQGR L NAIG+L +   YADAL  LG+ LE++A QE D            SC
Sbjct:   78 DSTSVLSSIKYHAEFTPSFSPEKFELPKAYYATAESVRDTLIINWNATYEFYEKMNVKQAYYLSMEFLQGRALLNAIGNLGLTGPYADALTKLGYSLEDVARQEPDAALGNGGLGRLASC 197          
BLAST of CX295634 vs. ExPASy Swiss-Prot
Match: PHSL_VICFA (Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Vicia faba GN=PHO1 PE=2 SV=2)

HSP 1 Score: 126.716 bits (317), Expect = 1.197e-28
Identity = 61/120 (50.83%), Postives = 81/120 (67.50%), Query Frame = 1
Query:  352 EPSAIASNISYHVQYSPHFSPTKFEPEQAFFATAESVRDRLIQQWNETYHHFNKVDPKQTYYLSMEFLQGRTLTNAIGSLDIQNAYADALNNLGHVLEEIAEQEKDXXXXXXXXXXXXSC 711
            + ++I S+I YH +++P FSP KFE  QAF ATA+SVRD LI  WN TY ++ K++ KQ YYLSMEFLQGR L NAIG+L++   YA+AL+ L + LE++A QE D            SC
Sbjct:   93 DTTSIVSSIKYHAEFTPLFSPEKFELPQAFIATAQSVRDALIINWNATYDYYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSQLSYKLEDVAHQEPDAALGNGGLGRLASC 212          
BLAST of CX295634 vs. ExPASy Swiss-Prot
Match: PHS1_DICDI (Glycogen phosphorylase 1 OS=Dictyostelium discoideum GN=glpV PE=1 SV=3)

HSP 1 Score: 95.5153 bits (236), Expect = 2.956e-19
Identity = 46/106 (43.40%), Postives = 73/106 (68.87%), Query Frame = 1
Query:  352 EPSAIASNISYHVQYSPHFSPTKFEPEQAFFATAESVRDRLIQQWNETYHHFNKVDPKQTYYLSMEFLQGRTLTNAIGSLDIQNAYADALNNLGHVLEEIAEQEKD 669
            +  +I  +I  HV+Y+   +   F+   A+  +A SVRDRLI++WNET  ++ + DPK+ YYLSMEFL GR+L NAI ++++++ Y +AL  LG  +E++ E+EKD
Sbjct:   40 DEDSIQKDILDHVEYTLARTKYNFDSFSAYQGSAYSVRDRLIERWNETQQYYTERDPKRVYYLSMEFLMGRSLQNAIYNMNLKDEYHNALLELGFEMEDLYEEEKD 145          
BLAST of CX295634 vs. ExPASy Swiss-Prot
Match: PYGB_HUMAN (Glycogen phosphorylase, brain form OS=Homo sapiens GN=PYGB PE=1 SV=5)

HSP 1 Score: 80.1073 bits (196), Expect = 1.285e-14
Identity = 39/81 (48.15%), Postives = 53/81 (65.43%), Query Frame = 1
Query:  427 PEQAFFATAESVRDRLIQQWNETYHHFNKVDPKQTYYLSMEFLQGRTLTNAIGSLDIQNAYADALNNLGHVLEEIAEQEKD 669
            P   FFA A +VRD L+ +W  T  H+ + DPK+ YYLS+EF  GRTL N + +L +QNA  +A+  LG  LEE+ E E+D
Sbjct:   49 PRDYFFALAHTVRDHLVGRWIRTQQHYYERDPKRIYYLSLEFYMGRTLQNTMVNLGLQNACDEAIYQLGLDLEELEEIEED 129          
The following BLAST results are available for this feature:
BLAST of CX295634 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 29
Match NameE-valueIdentityDescription
PHSH_VICFA8.542e-4370.83Alpha-glucan phosphorylase, H isozyme OS=Vicia fab... [more]
PHSH_ARATH5.537e-4260.42Alpha-glucan phosphorylase, H isozyme OS=Arabidops... [more]
PHSH_SOLTU2.104e-4166.94Alpha-glucan phosphorylase, H isozyme OS=Solanum t... [more]
PHSH_WHEAT3.968e-4065.83Alpha-glucan phosphorylase, H isozyme OS=Triticum ... [more]
PHSL_IPOBA2.182e-3052.50Alpha-1,4 glucan phosphorylase L isozyme, chloropl... [more]
PHSL1_SOLTU1.847e-2951.67Alpha-1,4 glucan phosphorylase L-1 isozyme, chloro... [more]
PHSL2_SOLTU5.375e-2950.83Alpha-1,4 glucan phosphorylase L-2 isozyme, chloro... [more]
PHSL_VICFA1.197e-2850.83Alpha-1,4 glucan phosphorylase L isozyme, chloropl... [more]
PHS1_DICDI2.956e-1943.40Glycogen phosphorylase 1 OS=Dictyostelium discoide... [more]
PYGB_HUMAN1.285e-1448.15Glycogen phosphorylase, brain form OS=Homo sapiens... [more]

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Properties
Property NameValue
Genbank descriptionC05074C07SK DroRLeaf1 Citrus clementina cDNA clone C05074C07, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295634 ID=CX295634; Name=CX295634; organism=Citrus clementina; type=EST; length=712bp
GATTCGGCCGAGGCCTCGCTCTCGTAGCAATGACCTCTATTCACTCACTC
ACTCTCGTGCGATCGTGATCGTCAGTCTCTGATATTTTTTTTCCCTTTAG
AAACACAATCGCTTCAAAGCTCTTGTTCGCTTCCTCTTCTTAGCATATTC
TTTCTCACTCCAATACTTTCTTTATAACAATATCTTCTCTTACACTTCAT
TTACGTTTTAAACTGCTTGCCGTTTCGTGTAGCTTCTGAAACAAGCAAGT
AATTTAATTTGTGGAATCAAATGGCGGATGCGAAAGCAAACGGAAAGAAT
GAGGCGGCCAAACTGGCGAAAATTCCGGCGGCTGCGAATCCATTGGCTAA
TGAACCATCGGCGATTGCATCAAATATAAGTTACCACGTGCAGTACAGTC
CTCATTTCTCGCCGACTAAGTTCGAGCCGGAGCAAGCTTTCTTTGCCACG
GCGGAGAGTGTCCGCGATCGTCTTATTCAACAATGGAATGAGACATACCA
CCATTTTAATAAAGTTGATCCGAAGCAAACATACTACCTATCAATGGAAT
TTCTTCAAGGAAGGACTTTGACTAATGCAATTGGCAGTTTGGACATTCAG
AATGCATATGCTGATGCTTTAAATAATTTGGGGCATGTCCTTGAGGAGAT
AGCTGAACAGGAAAAAGATGCTGCACTAGGAAATGGTGGGCTGGGCAGGC
TAGCTTCATGCT
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