Developing fruit peel at 38 DAFB

Overview
Library NameDeveloping fruit peel at 38 DAFB
Unique NameDeveloping fruit peel at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The entire peel (including both flavedo and albedo) was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit peel at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
DR403942DR403942EST
DR403943DR403943EST
DR403944DR403944EST
DR403945DR403945EST
DR403946DR403946EST
DR403947DR403947EST
DR403948DR403948EST
DR403949DR403949EST
DR403950DR403950EST
DR403951DR403951EST
DR403952DR403952EST
DR403953DR403953EST
DR403954DR403954EST
DR403955DR403955EST
DR403956DR403956EST
DR403957DR403957EST
DR403958DR403958EST
DR403959DR403959EST
DR403960DR403960EST
DR403961DR403961EST
DR403962DR403962EST
DR403963DR403963EST
DR403964DR403964EST
DR403965DR403965EST
DR403966DR403966EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageYoung fruit sample-collected June 17, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The entire peel (including both flavedo and albedo) was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type