CF832741

Overview
NameCF832741
Unique NameCF832741
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length706
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_HORVU (Xylose isomerase OS=Hordeum vulgare GN=XYLA PE=1 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 3.892e-64
Identity = 121/144 (84.03%), Postives = 128/144 (88.89%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            GNIDANTGDPQVGWDTD+FLTDI EATLIM SV+KN GLAPGGFNF AKLRRESTDVEDLFIAHI GMDT+ARG R   KLIEDGSL ELVRKRYQSFD+EIG  IEAGK DFE LEKKA+EWGEP VPS KQELAEM+FQSA+
Sbjct:  336 GNIDANTGDPQVGWDTDEFLTDISEATLIMSSVVKNDGLAPGGFNFYAKLRRESTDVEDLFIAHISGMDTMARGRRNVVKLIEDGSLDELVRKRYQSFDTEIGAMIEAGKGDFETLEKKALEWGEPTVPSGKQELAEMLFQSAL 479          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_ARATH (Xylose isomerase OS=Arabidopsis thaliana GN=XYLA PE=2 SV=2)

HSP 1 Score: 242.276 bits (617), Expect = 1.932e-63
Identity = 117/144 (81.25%), Postives = 133/144 (92.36%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            GNIDANTGD Q GWDTDQFLTD+GEAT++M+SVIKNGG+APGGFNFDAKLRRESTDVEDLFIAHI GMDT+ARGLR A K++E+GSL ELVRKRY ++DSE+G++IE GKADFE LEKKA E+GEPKV SAKQELAEMIFQSA+
Sbjct:  334 GNIDANTGDAQTGWDTDQFLTDVGEATMVMMSVIKNGGIAPGGFNFDAKLRRESTDVEDLFIAHISGMDTMARGLRNAVKILEEGSLSELVRKRYATWDSELGKQIEEGKADFEYLEKKAKEFGEPKVSSAKQELAEMIFQSAM 477          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_THEP1 (Xylose isomerase OS=Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) GN=xylA PE=3 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 6.476e-35
Identity = 76/144 (52.78%), Postives = 97/144 (67.36%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            G+IDAN GD  +GWDTDQF T+I + TL M  VIK GG   GG NFDAK+RR S  VEDLFI HIVGMDT A G + A KL++D  L   + ++Y+SF   IG++I  GKADFE LE+  ++  + ++PS KQE  E +  S I
Sbjct:  293 GSIDANQGDLLLGWDTDQFPTNIYDTTLAMYEVIKAGGFTKGGLNFDAKVRRASYKVEDLFIGHIVGMDTFALGFKIAYKLVKDEVLDRFIEEKYRSFKEGIGKEIVEGKADFEKLEEYIIDKEDIELPSGKQEYLESLLNSYI 436          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_THENN (Xylose isomerase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E) GN=xylA PE=3 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.461e-34
Identity = 74/144 (51.39%), Postives = 95/144 (65.97%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            G+IDAN GD  +GWDTDQF T++ + TL M  VIK GG   GG NFDAK+RR S  VEDLFI HI GMDT A G + A KL++DG L + + ++Y+SF   IG+ I  GK DFE LE+  ++    ++PS KQE  E +  S I
Sbjct:  293 GSIDANQGDLLLGWDTDQFPTNVYDTTLAMYEVIKAGGFTKGGLNFDAKVRRASYKVEDLFIGHIAGMDTFALGFKVAYKLVKDGVLDKFIEEKYRSFREGIGRDIVEGKVDFEKLEEYIIDKETIELPSGKQEYLESLINSYI 436          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_THENE (Xylose isomerase OS=Thermotoga neapolitana GN=xylA PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.461e-34
Identity = 74/144 (51.39%), Postives = 95/144 (65.97%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            G+IDAN GD  +GWDTDQF T++ + TL M  VIK GG   GG NFDAK+RR S  VEDLFI HI GMDT A G + A KL++DG L + + ++Y+SF   IG+ I  GK DFE LE+  ++    ++PS KQE  E +  S I
Sbjct:  293 GSIDANQGDLLLGWDTDQFPTNVYDTTLAMYEVIKAGGFTKGGLNFDAKVRRASYKVEDLFIGHIAGMDTFALGFKVAYKLVKDGVLDKFIEEKYRSFREGIGRDIVEGKVDFEKLEEYIIDKETIELPSGKQEYLESLINSYI 436          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_BACA2 (Xylose isomerase OS=Bacillus amyloliquefaciens (strain FZB42) GN=xylA PE=3 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 2.461e-34
Identity = 72/144 (50.00%), Postives = 96/144 (66.67%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            G++DAN GDP +GWDTD+F TD+  ATL M  ++KNGGL  GG NFDAK+RR S + +DL  AH+ GMDT ARGL+ A KLIED    ++++ RY+SF   IG +I  G+ADF+ LE+ A+     K  S +QE  + I    I
Sbjct:  299 GSVDANQGDPLLGWDTDEFPTDLYSATLAMYEILKNGGLGSGGLNFDAKVRRSSFEPDDLLYAHVAGMDTFARGLKAARKLIEDRVFEDVIQHRYRSFTEGIGLEIAEGRADFKTLEQYALNNQPIKNESGRQERLKAILNQYI 442          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_THESA (Xylose isomerase OS=Thermoanaerobacter saccharolyticum GN=xylA PE=1 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 3.214e-34
Identity = 74/139 (53.24%), Postives = 92/139 (66.19%), Query Frame = -3
Query:  288 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMI 704
            G+IDANTGD  +GWDTDQF TDI   TL M  VIK GG   GG NFDAK+RR S + EDLF+ HI GMD  A+G + A KL++DG   + + +RY S+   IG  I +GKADF+ LEK A+E  +    S +QEL E I
Sbjct:  293 GSIDANTGDMLLGWDTDQFPTDIRMTTLAMYEVIKMGGFDKGGLNFDAKVRRASFEPEDLFLGHIAGMDAFAKGFKVAYKLVKDGVFDKFIEERYASYKEGIGADIVSGKADFKSLEKYALEHSQIVNKSGRQELLESI 431          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_THESQ (Xylose isomerase OS=Thermotoga sp. (strain RQ2) GN=xylA PE=3 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 4.198e-34
Identity = 74/144 (51.39%), Postives = 95/144 (65.97%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            G+IDAN GD  +GWDTDQF T+I + TL M  VIK GG   GG NFDAK+RR S  VEDLFI HI GMDT A G + A KL +DG   + + ++Y+SF   IG++I  GK DFE LE+  ++  + ++PS KQE  E +  S I
Sbjct:  293 GSIDANQGDLLLGWDTDQFPTNIYDTTLAMYEVIKAGGFTKGGLNFDAKVRRASYKVEDLFIGHIAGMDTFALGFKIAYKLAKDGVFDKFIEEKYRSFKEGIGKEIVEGKTDFEKLEEYIIDKEDIELPSGKQEYLESLLNSYI 436          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_THEMA (Xylose isomerase OS=Thermotoga maritima GN=xylA PE=3 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 4.198e-34
Identity = 74/144 (51.39%), Postives = 95/144 (65.97%), Query Frame = -3
Query:  273 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWGEPKVPSAKQELAEMIFQSAI 704
            G+IDAN GD  +GWDTDQF T+I + TL M  VIK GG   GG NFDAK+RR S  VEDLFI HI GMDT A G + A KL +DG   + + ++Y+SF   IG++I  GK DFE LE+  ++  + ++PS KQE  E +  S I
Sbjct:  293 GSIDANQGDLLLGWDTDQFPTNIYDTTLAMYEVIKAGGFTKGGLNFDAKVRRASYKVEDLFIGHIAGMDTFALGFKIAYKLAKDGVFDKFIEEKYRSFKEGIGKEIVEGKTDFEKLEEYIIDKEDIELPSGKQEYLESLLNSYI 436          
BLAST of CF832741 vs. ExPASy Swiss-Prot
Match: XYLA_HAES2 (Xylose isomerase OS=Haemophilus somnus (strain 2336) GN=xylA PE=3 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 4.198e-34
Identity = 76/143 (53.15%), Postives = 95/143 (66.43%), Query Frame = -3
Query:  279 GNIDANTGDPQVGWDTDQFLTDIGEATLIMLSVIKNGGLAPGGFNFDAKLRRESTDVEDLFIAHIVGMDTVARGLRTAAKLIEDGSLPELVRKRYQSFDSEIGQKIEAGKADFEMLEKKAMEWG-EPKVPSAKQELAEMIFQS 704
            G+IDAN GDPQ+GWDTDQF   + E TL M  ++K GG   GGFNFDAK+RR+STD  DLF AHI  MD +A  L+ AAK+IED +L ++V  RY  +D E+GQKI +GKA  E L K     G +PK  S +QE  E +  S
Sbjct:  293 GSIDANRGDPQLGWDTDQFPNSVEENTLAMYEILKAGGFTTGGFNFDAKIRRQSTDPYDLFHAHIGAMDVLALSLKRAAKMIEDQTLQKVVDNRYAGWDQELGQKILSGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNS 435          
The following BLAST results are available for this feature:
BLAST of CF832741 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 172
Match NameE-valueIdentityDescription
XYLA_HORVU3.892e-6484.03Xylose isomerase OS=Hordeum vulgare GN=XYLA PE=1 S... [more]
XYLA_ARATH1.932e-6381.25Xylose isomerase OS=Arabidopsis thaliana GN=XYLA P... [more]
XYLA_THEP16.476e-3552.78Xylose isomerase OS=Thermotoga petrophila (strain ... [more]
XYLA_THENN2.461e-3451.39Xylose isomerase OS=Thermotoga neapolitana (strain... [more]
XYLA_THENE2.461e-3451.39Xylose isomerase OS=Thermotoga neapolitana GN=xylA... [more]
XYLA_BACA22.461e-3450.00Xylose isomerase OS=Bacillus amyloliquefaciens (st... [more]
XYLA_THESA3.214e-3453.24Xylose isomerase OS=Thermoanaerobacter saccharolyt... [more]
XYLA_THESQ4.198e-3451.39Xylose isomerase OS=Thermotoga sp. (strain RQ2) GN... [more]
XYLA_THEMA4.198e-3451.39Xylose isomerase OS=Thermotoga maritima GN=xylA PE... [more]
XYLA_HAES24.198e-3453.15Xylose isomerase OS=Haemophilus somnus (strain 233... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02F15_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02F15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832741 ID=CF832741; Name=CF832741; organism=Citrus sinensis; type=EST; length=706bp
CTTTTTTTTTTTTCCAAATTGAACTTCGATCATTATTAAAGCAATGTTAT
TGACAAAAGATTTCAAGATCGTAGGATTCAAGTTTTATAACAGCTGTCAT
ATCGATAACTAAACAAGTTCTTTATAAAGCCTAAGCTAGACAAACATGTC
AAAATTTCCCCGCATGAAAAGACCCATTTCCCCAAGAGGGTTCACTGACT
GGAGACAAACATAACTTCAATTTGCCCCTCTCTCTTTATTTGTAAGTTCT
GCTTCGGAGCAGCCGCTATCTATATTGCAGATTGGAAGATCATTTCTGCA
AGTTCCTGCTTAGCAGAGGGAACCTTGGGTTCTCCCCATTCCATGGCCTT
CTTCTCAAGCATTTCAAAATCAGCCTTACCAGCCTCTATTTTTTGCCCAA
TCTCTGAGTCAAAGCTCTGGTATCGCTTGCGGACAAGCTCCGGTAGAGAA
CCATCCTCAATCAGCTTGGCAGCAGTTCGAAGTCCACGAGCAACGGTATC
CATTCCAACAATATGAGCAATGAACAAGTCTTCAACGTCAGTGCTCTCTC
TTCGTAATTTTGCATCAAAGTTGAATCCTCCAGGTGCCAAACCTCCATTT
TTAATCACACTGAGCATAATCAAAGTAGCCTCTCCAATATCTGTCAGGAA
TTGATCTGTATCCCAACCAACTTGAGGGTCACCAGTGTTTGCATCGATGT
TTCCTA
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